• Title/Summary/Keyword: shoot cluster

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In vitro propagation of Spathiphyllum floribundum cv. Cupid (Spathiphyllum floribundum cv. Cupid의 기내번식)

  • 한봉희;예병우;유희주;신지수
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.4
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    • pp.209-213
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    • 2001
  • Spathiphyllum floribundum cv. Cupid was micropropagated on LS medium containing various growth regulators. Shoot tips were cultured on media with different concentrations of thidiazuron (TDZ) or banzyladenine (BA). The medium containing 2.0 mg/L BA was most effective on the shoot multiplication and the formation of adventitious multi-shoot cluster from shoot tips among the different concentrations of TDZ and BA. The combined treatments of BA and paclobutrazol (PBZ) were stimulated the shoot multiplication and the formation of adventitious mu]ti-shoot clusters from shoot tips compared to the ones of BA alone. The adventitious multi-shoot clusters were culled with 5∼7 mm in size and cultured on media with BA or thidiazuron (TDZ). Shoot multiplication and shoot cluster proliferation from the shoot clusters were very favorable on media supplemented with 2.0∼3.0 mg/L BA. The shoots grew and rooted out vigorously on media with 2.0∼3.0 mg/L IBA. The rooted plantlets were acclimatized in the soil mixed with a rate of vermiculite 1 and perlite 1, and survived more than 95% in greenhouse.

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In Vitro Propagation of Lilium Asiatic Hybrid 'Hae Hwa' via the Formation of Shoot Clusters (신초 Cluster 형성에 의한 Lilium Asiatic Hybrid 'Hae Hwa'의 기내번식)

  • Han, Bong-Hee;Yu, Hee-Ju;Yae, Byeoung-Woo;Goo, Dae-Hoe
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.19-23
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    • 2002
  • This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid 'Hae Hwa'. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.

Promotion of in vitro growth and rooting of micropropagated shoots in Spathiphyllum floribundum by the addition of liquid medium (액체배지 첨가에 의한 Spathiphyllum floribundum 신초의 기내생육 및 발근 촉진)

  • 한봉희;예봉우;구대회;신지수
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.4
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    • pp.185-188
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    • 2001
  • This experiments were carrid out to examine the effects of liquid medium addition in same vessels on shoot elongation and rooting, and soil survival of plantlets after the shoot cluster sections of Spathiphyllum floribundum 'Cupid' were pre-cultured. The shoot clusters with 3 to 4 small shoots were proliferated on LS medium supplemented with 2.0 mg/L BA for 8 weeks, and then 15 mL of various kinds of liquid medium was added in the same vessels. The addition of 15 mL liquid medium containing l/2 MS macro elements, 50 g/L sucrose and 5.0∼10.0 g/L activated charcoal was significantly stimulated the elongation and rooting of proliferated shoots. The medium addition was resulted in the enhanced soil survival of plantlets.

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The Effect of Shoot Twist at Bloom on Fruitfulness and Fruit Quality of 'Campbell Early' Grapevine (개화기 신초비틀기에 의한 '캠벨얼리' 포도의 착립 및 과실품질)

  • Lee, Jae-Wung;Song, Myung-Kyu;Park, Jae-Sung;Lee, Yun-Sang;Hong, Eui-Yon;Han, eom-Hwa
    • Korean Journal of Plant Resources
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    • v.31 no.1
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    • pp.71-76
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    • 2018
  • This study was conducted to investigate the effect of shoot twist on fruitfulness and fruit quality of 'Campbell Early' grapevine. Proper pruning and training are essential to produce a good yield of high-quality fruit and to maintain the balance between vegetative growth and fruiting. The most common problem in spur-pruned 'Campbell Early' cultivar is that vigorous buds has low fruitfulness and thereby the shoot become more vigorous the following spring because of lower crop load. Therefore, shoot twists in very vigorous 'Campbell Early' canes (above 10.0 mm) were performed on the third nodes and the $7^{th}$ nodes of each shoot at 7 days before bloom and full bloom, respectively. Sprouting date, blooming date were not significantly different among the treatments while, harvesting date was delayed approximately 3 days. However, number of berries per cluster, cluster weight and fruitfulness were significantly higher in the shoot twist treatment on the third nodes than the control that was topping alone. Combination treatments of shoot twist and topping had an additive effect on increasing cluster weight resulting in higher increase of yield by 12.1 ㎏ per vine. These results indicated that the shoot twist on very vigorous canes of 'Campbell Early' grapevine for well fruitfulness seemed to be very effective.

Optimum management of tomato side shoot removal in winter (저온기 토마토 재배시 적정 측지관리방법 구명)

  • Kim, Sung Eun;Lee, Jae Eun;Sim, Sang Youn;Lee, Moon Haeng;Kim, Young Shik
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.309-313
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    • 2014
  • This research was conducted to establish recommendable side shoot management methods in the growth and yield of tomato in winter. A cherry tomato, $^{\circ}{\times}Unicorn^{\circ}{\pm}$ (Monsanto Korea, Korea) was cultivated in coconut coir in the form of bag as substrate. There were four treatments related to side shoot removal methods; 1) To remove all side shoots and also each one of three leaves which gives shade to each cluster attached below (UP-FL), 2) To remove all side shoots and also each one of three leaves which gives shade to each cluster only with fruit attached below (UP-FR), 3) To remove all side shoots (AS-All), and 4) To remain two leaves of each side shoot coming from right below each cluster (AS-Part). The number of malformed leaves were more in UP-FL, UP-FR, AS-All, and AS-Part in descending order, which showed the severe the removal of leaves the more the malformed leaves. The malformed leaves were diminished after the development stage the second or third cluster bloomed. The yields until fifth cluster were not different among the treatments. Therefore removal of side shoots and leaves recommends not to be done severely in early growth stage when tomato plants are cultivated in winter.

Micropropagation of Lilium longiflorum 'Geogia' by Using Bioreactor. (생물반응기를 이용한 Lilium longiflorum ′Geogil′의 대량번식)

  • Han, Bong-Hee;Suh, Eun-Jung;Yae, Byeoung-Woo;Yu, Hee-Ju
    • Journal of Plant Biotechnology
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    • v.31 no.3
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    • pp.197-201
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    • 2004
  • Shoot clusters were induced from bulb scales of Lilium longiflorum 'Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL$.$min$^{-1}$ injection of air was suitable in growth of bulblets.

Effects of Cluster and Flower Thinning on Yield and Fruit Quality in Highbush 'Jersey' Blueberry (적방.적화 처리가 하이부쉬 'Jersey' 블루베리 과실의 수량과 품질에 미치는 영향)

  • Kim, Jin-Gook;Ryou, Myung-Sang;Jung, Sung-Min;Hwang, Yong-Soo
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.392-396
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    • 2010
  • This research focused on determining the effectiveness of cluster and/or flower thinning in highbush 'Jersey' blueberry on the yield and fruit quality. The total yield and quality of fruit were compared between thinning methods of control (no thinning), 1/3 cluster thinning, 1/3 cluster thinning + 1/2 flower thinning, 2/3 cluster thinning, and 2/3 cluster thinning + 1/2 flower thinning per each bearing shoot, respectively. Thinning strength significantly affected the yield of bearing shoot and, thus, total yield of control and 1/3 cluster thinning was higher than others. Both cluster and flower thinning, however, significantly affected the fruit growth resulting in the increase of fruit weight, length and width. Even total yield was similar between control and 1/3 cluster thinning, more larger fruit (> 1.6 g) were produced by 1/3 cluster thinning treatment, indicating that flower and/or cluster thinning contributed to the increase of individual fruit growth. Unlike fruit growth, fruit quality was less affected except total soluble solid contents. Total soluble solid level significantly increased in treatments (2/3 cluster thinning, 2/3 cluster thinning + 1/2 flower thinning) with yield decrease. No significant difference in levels of acidity and firmness of fruit was found. Results indicated that fruit growth and total yield of highbush blueberry was more influenced by the thinning strength regardless of cluster or flower than internal quality of fruit such as solid and acid levels.

Induction of Shoot Primordium in Culture of Garlic (Allium sativum L.) (마늘 배양에 있어서 신초원기 유도)

  • Choi Joo-Soo;Lee Bok-Kyu;Huh Man-Kyu
    • Journal of Life Science
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    • v.16 no.3 s.76
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    • pp.459-463
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    • 2006
  • Cultivated garlic, Allium sativum L. is economically important for leaves and bulbs, which historically were used in Korea for spices and condiments of Korean food as well as medicine crops. This experiment was carried out to investigate the effect of development and differentiation on culture of A. sativum (cv: white 6) by explant position, hormone composition and sucrose concentration in culture media. Culture method was investigated to induce shoot primordium. Culture efficiency was better with lower tissue of foliage leaf in explant position and on the medium with NAA 0.02 + BAP 1.0 mg/l in hormone composition than any other. Precocious shoot and callus were induced from shoot apex. Shoot was efficiently differentiated on 4,000 mg/l sucrose with increasing concentration of BAP. Shoot primordium was also induced with liquid rotary culture by histological observation. Rhizoid was induced from callus tissue cluster on medium with NAA 0.02 + BAP 2.0 mg/l.

Ecological Study of Pachynematus itoi Okutani (Hymenoptera: Tenthredinidae) (낙엽송잎벌 (Pachynematus itoi Okutani) (Hymenoptera: Tenthredinidae)의 생태학적 연구)

  • Park, Ji-Doo;Park, Il-Kwon
    • Korean journal of applied entomology
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    • v.49 no.1
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    • pp.17-22
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    • 2010
  • Pachynematus itoi Okutani emerges three times a year, and overwinters as a pre-pupa in cocoon. First emergence of P. itoi was from 7th May to third June. Second and third emergence was from 20th June to 13th July and fourth to 25th August, respectively. Mean oviposited egg number was 59, and egg period was about 9 days. Hatching rate was >90%. Oviposited egg number was the highest in the first needle cluster of short shoot followed by second and third. P. itoi did not oviposit on single needle (long shoot) of Larix leptolepis (Sieb. et Zucc.). Larva had five instars, and larval period was about 20 days. Larva prefer needle cluster of short shoot to single needle of long shoot. Mature larva descended from host tree and pre-pupa in cocoon overwinter at topsoil.

Effects of pruning intensity and diameter of bearing mother branches on the growth and berry quality in 'Kyoho' grapevines ('거봉' 포도의 전정강도와 결과모지 굵기가 생육 및 과실품질에 미치는 영향)

  • Song, Gi-Cheol
    • Journal of Agricultural Extension & Community Development
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    • v.7 no.1
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    • pp.167-173
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    • 2000
  • This study was carried out to investigate suitable pruning methods to avoid poor berry setting of 'Kyoho' grapevine in Suwon and Anseong areas. Light pruning increased percentage of bud bursts and number of shoots per bearing mother branches(BMB), cluster weight and percentage of berry sating but it decreased shoot length, number of leaves and, leaf area and leaf weight. There was little difference in soluble solids, titratable acidity, and anthocyanin contents of berries by the treatment of either light or heavy pruning. Shoot length became longer as diameter of BMB thickened, leaf area and chlorophyll content also became larger and diameter of BMB. Total carbohydrate and N, P, K, Ca, and Mg contents showed significantly little difference, but total carbohydrate and Ca contents showed a tendency of continuous increase as diameter of BMB thinned. Number of seeded berries per cluster became fewer as BMB thinned but percentage of berry setting showed an increasing tendency. Cluster weight, berry weight, berry number per cluster, soluble solids, anthocyanin content were higher in case of around 1cm of diameters of BMB.

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