Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Effect of Light and Scale Explant Conditions on Propagation Efficiency in Lilium callosum Scale Culture

땅나리 기내 인편 배양시 광환경과 배양 절편체의 조건이 증식 효율성에 미치는 영향

  • Park, Ji Young (Dept. of Horticulture, Seoul National University) ;
  • Yoo, Yong Kweon (Dept. of Horticultural Breeding, Mokpo National University) ;
  • Jeong, Jeong Hag (Dept. of Horticulture and Breeding, Andong National University) ;
  • Kim, Ki Sun (Dept. of Horticulture, Seoul National University)
  • Published : 1998.10.31
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Abstract

Series of in vitro experiments in Lilium callosum were conducted to investigate efficient multiplication through finding the optimal cultural environment, and organogenic capability of cultural explants, and then to determine the progressive method for enhancing bulblet growth in Lilium callosum scale culture. Twenty-four hr photoperiod was most effective for the growth of bulblet and the formation of other organs. Optimum light intensity for bulblet growth was 2,500~5,000 Lux. When bulbets were subcultured, growth of bulblets were enhanced by removing excessive leaf blade. Number of bulblets per scale increased as mother scale size increased, whereas diameter of bulblet from the small size mother scale increased. Bulblet formation and development was induced when explants were placed above the medium to be exposed to more light.

본 실험은 자생나리인 땅나리의 기내 대량생산 체계를 확립하기 위한 기초 연구로서 배양환경중 광환경 및 배양 절편체의 분화 능력 조건을 유도하여 자구의 생장을 촉진시킬 수 있는 방법을 모색하기 위하여 실시하였다. 광조건은 24시간 지속적인 일장처리와 2,500~5,000 Lux 정도의 광도가 자구의 발달에 효과적이었다. 계대배양시 번무한 지상부를 자구의 연접부위까지 완전히 절단해 줌으로써 전반적인 식물체 생육을 촉진시킬 수 있었고, 절편체의 크기가 클수록 자구수가 많았으나, 자구의 비대는 모인편의 크기가 작은 처리구에서 약간 우수하였다. 광을 많이 필요로 하는 땅나리는 기내 인편 배양시 치상방법을 배지면 위에 노출시켜 치상하는 것이 자구생산면에 있어서 효율적이었다.

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Acknowledgement

Supported by : Hansol Institute of Science & Technology