Randomly Amplified Polymorphic DNA (RAPD) Analysis on Compatibility of Korean Native Cymbidium goeringii with Other Cymbidium Species

한국춘란(韓國春蘭)과 다른 Cymbidium간의 교배친화성(交配親和性)에 대한 RAPD 분석

  • Published : 1998.10.31

Abstract

Cymbidium goeringii native to Korea and other orchid plants were pot-grown from spring to autumn under the greenhouse conditions, and were subjected to artificial pollination to elucidate the compatibility by revealing viable seed formation. A notable compatibility was found when Cym. goeringii was selfed and was crossed with either Cym. ensifolium, Cym. kanran, Cym. sinense, Cym. sinense for. albo-jucundissimum, Cym. 'Crystal Cherry Angel', or Cym. 'Anmitsu Hime'. Cym. goeringii, however, did not show such compatibility when crossed with either Cym. faberi, Cym. aloifolium, Dendrobium chrysotoxum, or Phalaenopsis spp. RAPD analysis indicated that taxa relationship between Cym. goeringii and either Cym. faberi or Cym. aloifolium (respective chromosome number, 2n=40) was distant, showing no compatibility, and even more distant in the case of cross-pollination between Cym. goeringii and either Dendrobium chrysotoxum or Phalaenopsis spp. having different chromosome number from all Cymbidium species.

온실에서 춘란(春蘭)과 다른 공시 난과식물을 분식(盆植)하고, 인위적인 타가수분을 실시하여 교배친화성을 검정한 결과, 춘란은 자가수분은 물론 Cym. ensifolium, Cym. kanran, Cym. sinense, Cym. sinense for. albo-jucundissimum, Cym. 'Crystal Cherry Angel', Cym. 'Anmitsu Hime'와 교배친화성을 보였으나 Cym. faberi, Cym. aloifolium, Cym. 'Husky Honey', Dendrobium chrysotoxum, Phalaenopsis spp.는 교배친화성을 나타내지 않았다. RAPD 분석결과, 춘란과 동일한 염색체수를 가진 Cym. faberi, Cym. aloifolium, Cym. 'Husky Honey'는 교배친화성을 보인 다른 공시 Cymbidium들보다 유전적인 근연관계가 멀었으며, 염색체수가 다른 Dendrobium chrysotoxum와 Phalaenopsis spp.는 상대적인 근연관계가 훨씬 먼 것으로 나타났다.

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Acknowledgement

Supported by : Korean Science and Engineering Foundation (KOSEF)