• Journal of Ginseng Research
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• v.40 no.2
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• pp.127-134
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• 2016

Background: Rhizospheric fungi play an essential role in the plantesoil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China. Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng. Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus. Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.

• Journal of the Korean Society for Nondestructive Testing
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• v.25 no.2
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• pp.95-102
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• 2005

There are many tube and pipeline in nuclear power plant under high temperature and high pressure. Erosion and corrosion defects were expected on these tube and pipe-line by environmental and mechanical factors. These erosion and corrosion defects ran be evaluated by ultrasonic technique. In these study, Scanning Laser Source(SLS) technique was applied to detect defect and construct image. This technique also makes detection possible on rough and curved surfaces such as tube and pipe-line by scanning. Conventional ultrasonic scanning technique requires immersion of specimen or water jet for transferring ultrasonic wave between transducer and specimen. However, this SLS technique does not need contacting and couplant to generate surface wave and to get flaw images. Therefore, this SLS technique has several advantages, for complicated production inspection, non-contact, remote from specimen, and high resolution. In this study, SLS images were obtained with various conditions of generation laser ultrasound and receiving in order to enhance detectability of flaws on the tube. Stress corrosion cracks were produced on tube and images of stress corrosion cracks were constructed by using SLS technique.

• Tribology and Lubricants
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• v.30 no.5
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• pp.256-264
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• 2014

Enhancing the value of fine chemicals based on biomass resources is an important objective for addressing environmental and other concerns such as demand for renewable or green products, as well as from the political perspective to reduce dependence on fossil feedstock associated with the use of petroleum-based products. Based on these considerations, we studied the synthesis of estolide using waste plant-based oil materials and their application as lubricants and pour point depressants. Five estolides were prepared by varying molar ratio of palmitic acid (PA) to oleic acid (OA) using a reaction time of 48 h. The estolides were characterized by size exclusion chromatography (SEC) and nuclear magnetic resonance (NMR). The isolated yields were in the range of 57-78 % and purity was 93-97%, showing iodine values of 18.2-37.8, total acid numbers (TANs) of 75.6-94.2 mg KOH/g and estolide numbers (ENs) of 1.2-1.8. Increasing the ratio of OA to PA in the synthesis decreased the kinematic viscosity and clouding point of the estolides. Four ball wear test of the estolides as a base oil demonstrated that the wear scar diameter (WSD) of the estolides was significantly lower (0.320-0.495 mm) than the WSD of general base oils such as 150N and Yubase (0.735 and 0.810 mm, respectively), indicating better wear resistance of the estolides. However, the lubricant property was found to be independent of the amount of OA in the estolides. These new materials are prospective candidates for application as a lubricant base oil.

• Journal of Radiation Protection and Research
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• v.24 no.4
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• pp.215-223
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• 1999

Characteristics of radiation field in the steam generator(S/G) water chamber of a PWR were investigated and the anticipated effective dose rates to the worker in the S/G chamber were evaluated by Monte Carlo simulation. The results of crud analysis in the S/G of the Kori nuclear power plant unit 1 were adopted for the source term. The MCNP4A code was used with the MIRD type anthropomorphic sex-specific mathematical phantoms for the calculation of effective doses. The radiation field intensity is dominated by downward rays, from the U-tube region, having approximate cosine distribution with respect to the polar angle. The effective dose rates to adults of nominal body size and of small body size(The phantom for a 15 year-old person was applied for this purpose) appeared to be 36.22 and 37.06 $mSvh^{-1}$) respectively, which implies that the body size effect is negligible. Meanwhile, the equivalent dose rates at three representative positions corresponding to head, chest and lower abdomen of the phantom, calculated using the estimated exposure rates, the energy spectrum and the conversion coefficients given in ICRU47, were 118, 71 and 57 $mSvh^{-1}$, respectively. This implies that the deep dose equivalent or the effective dose obtained from the personal dosimeter reading would be the over-estimate the effective dose by about two times. This justifies, with possible under- or over- response of the dosimeters to radiation of slant incidence, necessity of very careful planning and interpretation for the dosimetry of workers exposed to a non-regular radiation field of high intensity.

• Journal of Radiation Protection and Research
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• v.28 no.4
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• pp.271-279
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• 2003

Soil blocks were taken into culture boxes from 12 paddy fields within 5 km radii of Yonggwang and Ulchin NPPs and $^{90}Sr$ was applied to the surface water at a pre-transplanting stage and $1{\sim}2$ days before the start of heading. Following the pre-transplanting application, transfer factors were investigated for $2{\sim}4$ years. In the year of application, transfer factors $(m^2\;kg^{-1}-dry)\;of\;^{90}Sr$ applied before transplanting, showing no regionally distinguishable trend, varied with soils by a factor of about 2 with averages of $2.6{\times}10^{-4}$ for hulled seeds and $1.3{\times}10^{-2}$ for straw Transfer factors of $^{90}Sr$ applied shortly before heading were about 2 times greater than those applied before transplanting. Transfer factors tended to decrease with increasing soil pH and exchangeable Ca. Generic values of $^{90}Sr$ transfer factors in the year of deposition were proposed for the Korean paddy fields. In the second year compared with the first year, the transfer factor decreased more in Ulchin soils, which were on the whole higher in sand content, than in Yonggwang soils. For Yonggwang soils as a whole, the annual decrease in transfer factor was well described by an exponential equation with a half-life of about 2.2 years.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3519-3528
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• 2012

Inhibitory effects of Maclura amboinenesis Bl, one plant used traditionally for the treatment of cancers, on metastatic potential of highly metastatic B16F10 melanoma cells were investigated in vitro. Cell proliferation was assessed using the MTT colorimetric assay. Details of metastatic capabilities including invasion, migration and adhesion of B16F10 melanoma cells were examined by Boyden Chamber invasion and migration, scratch motility and cell attachment assays, respectively. The results demonstrated that n-hexane and chloroform extracts exhibited potent anti-proliferative effects (p<0.01), whereas the methanol and aqueous extracts had less pronounced effects after 24 h exposure. Bioactivity-guided chromatographic fractionation of both active n-hexane and chloroform extracts led to the isolation of two main prenylated xanthones and characterization as macluraxanthone and gerontoxanthone-I, respectively, their structures being identified by comparison with the spectral data. Interestingly, both exhibited potent effective effects. At non-toxic effective doses, n-hexane and chloroform extracts (10 and $30{\mu}g/ml$) as well as macluraxanthone and gerontoxanthone-I (3 and $10{\mu}M$) significantly inhibited B16F10 cell invasion, to a greater extent than $10{\mu}m$ doxorubicin, while reducing migration of cancer cells without cellular cytotoxicity. Moreover, exposure of B16F10 melanoma cells to high concentrations of chloroform ($30{\mu}g/ml$) and geratoxanthone-I ($20{\mu}M$) for 24 h resulted in delayed adhesion and retarded colonization. As insights into mechanisms of action, typical morphological changes of apoptotic cells e.g. membrane blebbing, chromatin condensation, nuclear fragmentation, apoptotic bodies and loss of adhesion as well as cell cycle arrest in the G1 phase with increase of sub-G1 cell proportions, detected by Hoechst 33342 staining and flow cytometry were observed, suggesting DNA damage and subsequent apoptotic cell death. Taken together, our findings indicate for the first time that active n-hexane and chloroform extracts as well as macluraxanthone and gerontoxanthone-I isolated from Maclura amboinensis Bl. roots affect multistep of cancer metastasis processes including proliferation, adhesion, invasion and migration, possibly through induction of apoptosis of highly metastatic B16F10 melanoma cells. Based on these data, M. amboinensis Bl. represents a potential candidate novel chemopreventive and/or chemotherapeutic agent. Additionally, they also support its ethno-medicinal usage for cancer prevention and/or chemotherapy.

• Proceedings of the Korea Contents Association Conference
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• 2004.05a
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• pp.553-561
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• 2004

It works as intermediation of communication of publics these days. Since 20 century, We, Koreans, have established new chaotic multi culture with traditional Korean culture and other different culture from everywhere. Meanwhile, we occupied the most powerful semi-conduct and IT indusry. Within those circumstance, people feel very confused in political, cultural and social aspect. The society armed with economy and popularization promotes material satisfaction with this potential possibility of anonymous masses. However, it results to cause loneliness, isolation, alienation, anonymity, non individuality and commodity of culture. In my work, such phenomenon reveals through human character in a city. People are exposed culture of consumption and surrounded and tempted by all those artificial and superficial atmosphere. Human are possessed and exposed to attractive products and visual images. Finally they make themselves stuck in their case of this world. People lose their own identify and shape of bodies. That is our portrait, who are living this moment. Also, this is a symbol that destroys this modern society. As a result, 1 consider such aspects through those elements above to think how to keep and rethink our identity and what to do for this world.

• Proceedings of the Korea Contents Association Conference
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• 2004.05a
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• pp.193-206
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• 2004

예로부터 빛고을로 인식되었던 광주가 근세에 이르러 의병활동, 광주학생운동, 5\ulcorner18민주화운동, 아직도 끝나지 않은 이념전쟁으로만 비추어질 수도 있는 지역의 이미지를 재정립하고, 도시 경쟁력의 증대를 위한 방안으로 CI사업 추진계획을 수립하고 3개년에 걸쳐(1999～2001) CI전략 개발, CI디자인 개발, 문화상품 개발, 도시환경디자인 개발 등을 추진하였다. 최근 커뮤니케이션에 있어서 캐릭터는 정보의 시각화라는 측면에서 다른 그래픽 심볼에 비해 설득적이라 할 수 있고, 기존의 심볼의 이미지 보완적 보조심볼로서의 개념이 아닌 독자적인 영역이 구축되어야 하므로 광주광역시 캐릭터 개발은 Character Identity 차원에서 메인캐릭터와 이를 각각의 용도에 맞춰 응용전개시키는 이벤트 캐릭터, 브랜드캐릭터로 구분하여 유형에 따라 다르게 적용되어야 할 적용성 및 활용성에 중점을 두고 개발되었다. 광주광역시의 메인캐릭터 개발컨셉은 "빛"과 "생명"으로 결정된 TCI이념을 활용하는 것과 광주의 상징 요소로서 "무등산"을 활용하는 두 가지 방향에서 개발이 진행되었으며, 1999년 10월의 TCI 추진회의 1차선정을 시작으로 광주시민을 포함한 전국민 설문조사와 2000년 4월의 의회설명회에 이르기까지 다양한 방법을 통해 캐릭터 개발안에 대한 평가와 검토/수정과정을 거쳐 기본캐릭터를 완성하게 되었다. 이를 통해 본 광주광역시의 캐릭터는 중장기적으로 치밀하게 계획되고 실천되어야 하며 변화하는 환경에 효율적으로 대응하는 구체적이고 실천적인 연구평가와 접근방법에 대한 연구 등으로 그 지역의 특징적인 문화를 상품화시키려는 노력이 캐릭터개발을 토대로 뒤따라야 한다. 촉진, 중요 연구 주제의 도출 및 연구 진행 계획을 수립하고, 국제적 산학 협력 강화를 통해 공동 연구를 도출하는 자리를 마련함. $\textbullet$본 학술회의에서 발표된 논문들을 취합하여, 논문집 및 CD-ROM을 제작하여 세계적 수준의 연구 결과를 알리고 이 분야에 관련된 학문, 기술 발전에 큰 도움이 됨.emove effectively radioactivity with in Adsorbent. As cleaning heavy water adsorbent and drying on each condition (temperature for drying and hours for cleaning). Because there is something to return heavy water adsorbent by removing impurities within adsorbent when it is dried o high temperature. After operating, we have been applying this research to the way to dispose heavy water adsorbent. Through this we could reduce solid waste products and the expense of permanent disposal of radioactive waste products and also we could contribute nuclear power plant run safely. According to the result we could keep the best condition of radiation s

• Journal of the Institute of Convergence Signal Processing
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• v.4 no.1
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• pp.15-26
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• 2003

Ultrasonic inspection methods are widely used for detecting flaws in materials. The signal analysis step plays a crucial part in the data interpretation process. A number of signal processing methods have been proposed to classify ultrasonic flaw signals. One of the more popular methods involves the extraction of an appropriate set of features followed by the use of a neural network for the classification of the signals in the feature space. This paper describes an alternative approach which uses the least mean square (LMS) method and expectation maximization (EM) algorithm with the model based deconvolution which is employed for classifying nondestructive evaluation (NDE) signals from steam generator tubes in a nuclear power plant. The signals due to cracks and deposits are not significantly different. These signals must be discriminated to prevent from happening a huge disaster such as contamination of water or explosion. A model based deconvolution has been described to facilitate comparison of classification results. The method uses the space alternating generalized expectation maximization (SAGE) algorithm In conjunction with the Newton-Raphson method which uses the Hessian parameter resulting in fast convergence to estimate the time of flight and the distance between the tube wall and the ultrasonic sensor Results using these schemes for the classification of ultrasonic signals from cracks and deposits within steam generator tubes are presented and showed a reasonable performances.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.267-272
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• 2012

Vitex trifolia L. is a full grown fruit of Vitex rotundifolia L. (Verbenaceae). It has been used for treating headache, dizziness, toothache and removal of fever as a traditional medicine in Korea. V. trifolia (500 g) were extracted three times with 80% aqueous MeOH at room temperature. The MeOH extract (38 g) was successively partitioned with n-hexane, EtOAc, n-BuOH and $H_2O$. Both n-hexane and EtOAc fractions showed more than 80% antifungal activity in vivo against several plant pathogens at 2000 ppm. Successive repeated silica gel, Sephadex LH-20, preparative TLC, and preparative HPLC of these fractions led to isolation of three compounds. Using mainly mass spectroscopy and nuclear magnatic resonance (NMR) spectroscopy, their chemical structures were determined as agnuside(1), chrysosplenol B(2), and artemetin(3). Compound 2 was isolated for the first time from V. trifolia. Study on in vitro and in vivo antifungal activities of the isolated compounds is in progress.