• Biomolecules & Therapeutics
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• 제22권4호
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• pp.295-300
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• 2014

The actin cytoskeleton plays an important role in macrophage-mediated inflammatory responses by modulating the activation of Src and subsequently inducing nuclear factor (NF)-${\kappa}B$ translocation. In spite of its critical functions, few papers have examined how the actin cytoskeleton can be regulated by the activation of toll-like receptor (TLR). Therefore, in this study, we further characterized the biological value of the actin cytoskeleton in the functional activation of macrophages using an actin cytoskeleton disruptor, cytochalasin B (Cyto B), and explored the actin cytoskeleton's involvement in morphological changes, cellular attachment, and signaling events. Cyto B strongly suppressed the TLR4-mediated mRNA expression of inflammatory genes such as cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-${\alpha}$, and inducible nitric oxide (iNOS), without altering cell viability. This compound also strongly suppressed the morphological changes induced by lipopolysaccharide (LPS), a TLR4 ligand. Cyto B also remarkably suppressed NO production under non-adherent conditions but not in an adherent environment. Cyto B did not block the co-localization between surface glycoprotein myeloid differentiation protein-2 (MD2), a LPS signaling glycoprotein, and the actin cytoskeleton under LPS conditions. Interestingly, Cyto B and PP2, a Src inhibitor, enhanced the phagocytic uptake of fluorescein isothiocyanate (FITC)-dextran. Finally, it was found that Cyto B blocked the phosphorylation of vasodilator-stimulated phosphoprotein (VASP) at 1 min and the phosphorylation of heat shock protein 27 (HSP27) at 5 min. Therefore, our data suggest that the actin cytoskeleton may be one of the key components involved in the control of TLR4-mediated inflammatory responses in macrophages.

• Journal of Microbiology
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• 제34권1호
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• pp.25-29
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• 1996

Since sequencing of randomly selected cDNA clones has been known to be a powerful approach to obtain information on gene expression pattern in specific cells or tissues, we have analyzed a 3'-directed cDNA library of vegetative mycelia of A. nidulans by single-pass sequencing of hundreds of randomly selected clones. Sequencing of 292 cDNA clones yielded 209 gene signatures (GSs) probably representing highly or lesser expressed genes in the vegetative mycelia. Among the 209 GSs, 25 (79 cDNA clones) appeared more than once and 184 only once. One GS appeared at a highest frequency of 6 times, 2 GSs5 times, 4 GSs 4 times, a GSs 3 times and 16 GSs twice. About 6.6% GSs comprizing of 13 GSs showed alternative polyadenylation. Among 23 redundant GSs, three were common in both mycelia and sexual organs, and 22 were probably mycelia-specific. Out of 209 GSs, 36 were identified in GenBank showing of 70% or greater similaritis. Only six GSs were for A. nidulans genes, and 13 GSs were of DNA or genes encoding cytoplasmic or organellar proteins. This pattern is similar to those in the human HepG2 cell line and in human colonic mucosa, although very few genes for nuclear proteins and for protein synthesis were in A. nidulans.

• Journal of Radiation Protection and Research
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• 제34권2호
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• pp.43-48
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• 2009

When the PDD (percentage depth dose) in the megavoltage beams is measured in the water phantom, the polarity and ion recombination effects of ionization chambers with depth in water are not usually taken into consideration. We try to investigate if those variations with depth should be taken into consideration or could be ignored for the thimble type semiflex ionization chamber (PTW $31010^{TM}$, SN 1551). According to the recommendation of IAEA TRS-398, the 4 representative depths of $d_s$, $d_{max}$, $d_{90}$ and $d_{50}$ were used for the electron beams. For the photon beams, the 4 depths were arbitrarily chosen for the photon beams, which were $d_s$, $d_{max}$, $d_{10}$ and $d_{20}$. For the high energy photon beam both polarity and ion recombination factors of the chamber with depth in water gives the good agreements within the maximum $\pm$0.2%, while the $C_{polS}$ with depth came within the maximum $\pm$ 0.4% and the $C_{IRS}$ within the maximum $\pm$0.6% in every electron beam used. This study shows that PDI (percentage depth ionization) could be a good approximation to PDD for the chamber used.

• Journal of the Korea Society of Computer and Information
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• 제26권7호
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• pp.1-7
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• 2021

In this paper, we propose a feature selection technique for multi-label classification. Many existing feature selection techniques have selected features by calculating the relation between features and labels such as a mutual information scale. However, since the mutual information measure requires a joint probability, it is difficult to calculate the joint probability from an actual premise feature set. Therefore, it has the disadvantage that only a few features can be calculated and only local optimization is possible. Away from this regional optimization problem, we propose a feature selection technique that constructs a low-rank space in the entire given feature space and selects features with sparsity. To this end, we designed a regression-based objective function using Nuclear norm, and proposed an algorithm of gradient descent method to solve the optimization problem of this objective function. Based on the results of multi-label classification experiments on four data and three multi-label classification performance, the proposed methodology showed better performance than the existing feature selection technique. In addition, it was showed by experimental results that the performance change is insensitive even to the parameter value change of the proposed objective function.

• Transactions of the Korean Society of Mechanical Engineers A
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• 제34권12호
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• pp.1837-1842
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• 2010

Studies on alternative energy have been carried out for many decades because of the accelerated exhaustion of fuel. While the efficacy of solar cells is still low in comparison with that of nuclear power, solar cells have been highlighted as potential sources of alternative energy because they are environmentally friendly and have a source of unlimited energy, namely, the sun. In this study, the optimum efficiency of solar cells was simulated as a function of the incident angle of sunlight and the geometric shapes of patterns using MATLAB and MathCAD software. The foremost efficiency of the solar cell was found to be 1.10 when the thickness and width of the patterns were in the range 25-$50{\mu}m$ and 50-$100{\mu}m$, respectively. To achieve the 25 um thick layer, 100,000 cps silver paste and 500 um orifice tip has been successfully implemented with Micro-Dispensing Deposition Writing.

• The Journal of the Korea institute of electronic communication sciences
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• 제13권1호
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• pp.35-44
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• 2018

Korea government aims to generate 20 percent of its electricity with clean, renewable energy by 2030, while reducing its reliance on fossil fuel and nuclear power plants. Technically, solar energy has resource potential that far exceeds the entire global energy demand. Solar energy industry has experienced phenomenal growth in recent years due to both technological improvements resulting in cost reductions and government policies for renewable energy development and utilization. Even though solar power generation has several advantages over other forms of electricity generation, the major problem is the requirement of land which is scarcely available in the local site and its cost. This study analyzes the available capacity of landing and floating solar plants for the case of chonnam province in korea. The results of design capacity show about 7.5GW for landing and 1.5GW for floating solar power plant. Also, with a purpose to comprehend intention-behaviour gap about acceptance of solar community, the solutions are suggested.

• Journal of the Institute of Electronics and Information Engineers
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• 제53권4호
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• pp.117-128
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• 2016

Installation of earth grounding system is essential to ensure personnel safety and correct operation of electrical equipment. Earth parameters, especially, soil resistivity has to be determined in designing an efficient earth grounding system. The most common applied technique to measure soil resistance is Wenner four-point method. Implementation of this method is expensive, time consuming and cumbersome as large set of measurements with variable electrode spacing are required to obtain a one dimensional resistivity plot. It is advantageous to have a method which is of low cost and provides fast measurements. In this perspective, electrical resistance tomography (ERT) is applied to estimate subsurface resistivity profile. Electrical resistance tomograms characterize the soil resistivity distribution based on the measurements from electrodes placed in the region of interest. The nonlinear ill-posed inverse problem is solved using iterated Gauss-Newton method with Tikhonov regularization. Through extensive numerical simulations, it is found that ERT offers promising performance in estimating the three-dimensional soil resistivity distribution.

• Journal of the Korean Society of Radiology
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• 제14권5호
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• pp.643-650
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• 2020

The materials with a high processiblity for radiation shielding, in particular for 3D printable materials, are highly demanding for producing robots working in nuclear plants and designing customized personal protection equipment. In this study, we suspend tungsten particles in a polymeric matrix of either PLA or ABS to compose tungsten-polymer composite filaments; PLA and ABS are widely used for conventional FDM-based 3D printing. The weight fraction of tungsten particles can be increased up to 50% without forming macroscopic aggregates. The composite filaments can be used to print 3D architectures with any shape and geometry. To demonstrate one of potential applications, we print parts for robot actuator and assemble them to protect PCB against gamma ray.

• Journal of the Korean Society of Safety
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• 제24권6호
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• pp.157-162
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• 2009

Radioactive medicines are used a lot owing to the increase of a PET-CT examination using glucose metabolism useful for the early diagnosis of diseases. Therefore, the spatial dose that is generated from patients and their surroundings causes the patients' guardians and health professional to be exposed to radiation. However, they get unnecessarily exposed to radiation because medical institutions lack in space for isolation and recognition of the examination. This research intended to examine the spatial dose rates by measuring the dose emitted from the patient for 48 hours to whom F-18 FDG was administered. The spatial dose rates that were measured 100cm away from the patient's body after F-18 FDG was injected were $65.88{\mu}$Sv/hr at 60-minute point, $45.13{\mu}$Sv/hr at 90-minute point, $9.88{\mu}$Sv/hr at 6-hour point, and $1.24{\mu}$Sv/hr at 12-hour point. When the dose that the guardian and health professional got was converted into the annual(240-day working) accumulative dose, it was examined that the guardian received 81.56 mSv/yr and health professional received 49.36mSv/yr. In addition, the result has revealed that the dose that the patient received from one time of PET-CT examination was 3.75mSv/yr, which is 1.5 times more when compared with the annual natural radiation exposure dose.

• Journal of Radiation Protection and Research
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• 제45권4호
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• pp.154-162
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• 2020

Background: Several studies have reported that wild mushrooms contain high amounts of radioactive cesium (137Cs). After the Fukushima Daiichi Nuclear Power Plant Accident, a significantly high concentration of 137Cs has been detected in wild mushrooms, and their consumption may be the cause of the chronic internal exposure of local consumers to radioactivity. Therefore, an accurate evaluation of the internal radioactivity resulting from mushroom ingestion is needed. Materials and Methods: The 137Cs elution rate through the cooking and digestion stages was evaluated using in vitro experiments. The edible mushroom Pleurotus djamor was taken as a sample for the experiments. The mushrooms were cultivated onto solid media containing 137Cs. We evaluated the internal dose based on the actual conditions using the elution rate data. For various cooking methods, the results were compared with those of other wild edible mushrooms. Results and Discussion: From the elution experiment through cooking, we proved that 25%-55% of the 137Cs in the mushrooms was released during soaking, boiling, or frying. The results of a simulated digestion experiment after cooking revealed that almost all the 137Cs in the ingested mushrooms eluted in the digestive juice, regardless of the cooking method. The committed effective dose was reduced by 20%-75% when considering the dissolution through the cooking process. Conclusion: We found that cooking lowers 137Cs concentration in mushrooms, therefore reducing the amount of radioactivity intake. Besides, since there were differences between mushroom types, we demonstrated that the internal exposure dose should be evaluated in detail considering the release of 137Cs during the cooking stages.