• 벤처창업연구
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• 제9권6호
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• pp.213-230
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• 2014

전 세계적으로 실업과 고용창출이 중요한 이슈로 부각됨에 따라, 고용률을 높이고 경제를 활성화하기 위하여 우리나라를 비롯한 많은 선진국에서는 다양한 창업활성화 정책을 수립 시행하고 있다. 창업활성화에 있어 중요한 요인은 기업가정신이므로, 예비창업자와 기창업자를 대상으로 창업동기, 기업가정신, 창업의지 및 기업성과에 관계를 규명한다면, 기업가정신을 증진시킬 수 있는 대상별 맞춤형 창업정책프로그램을 마련하는데 중요한 시사점을 제공할 수 있을 것이다. 이에 본 연구에서는 창업정책수혜를 받고 있는 예비창업자와 기창업자를 대상으로 창업동기요인을 규명하고, 창업동기요인이 기업가정신과 창업의지(예비창업자) 및 기업성과(기창업자)에 미치는 영향을 분석하였다. 기술통계와 신뢰도분석을 실시하였으며, 타당도 검증을 위해 요인분석을 실시하였고, 상호상관성을 보기위해 상호관계를 분석하였으며, 최종 회귀분석을 통한 검증으로 요인들 간의 영향력을 분석하였다. 예비창업자의 경우, 총 202명을 대상으로 분석하였으며, 그 결과 자기효능감, 인적네트워크, 경제적요인, 정부정책은 기업가정신에 영향을 미치는 것으로 나타났다. 또한, 자기효능감, 창업교육, 경제적요인, 정부정책은 창업의지에 정의 영향을 미치는 것으로 나타났다. 기창업자는 총 212명을 대상으로 분석하였으며, 자기효능감, 인적네트워크, 경제적요인이 기업가정신에 영향을 미치는 것으로 나타났다. 그리고 자기효능감은 재무적성과와 비재무적성과에, 창업교육은 재무적성과와 기술적성과에 인적네트워크는 비재무적성과에, 경제적요인은 재무적성과와 비재무적성과에, 정부정책은 재무적성과와 기술적성과에 각각 정의영향을 미치는 것으로 나타났다.

• 융합정보논문지
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• 제10권8호
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• pp.77-86
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• 2020

이 연구는 코로나 19 확산에 따른 WHO의 마스크 사용 지침에 대한 한국 보건당국의 이행 및 불이행 내용을 분석하고 그것의 원인과 대안 선택의 결과를 도출하여 전염병 예방에서 지침의 한계와 연구의 함의를 제시하는데 목표가 있다. 이를 위해 국내·외 마스크 관련 문헌들을 고찰하고 WHO의 마스크 지침과 한국 보건당국의 마스크 권고사항을 분석대상으로 한다. 분석 결과는 다음과 같다. 첫째, 한국의 보건당국은 두 차례의 권고 사항에서 WHO의 "제한된 마스크 사용자 관점"을 기본적으로 수용하고 적용해왔다. 둘째, 한국의 보건당국은 마스크 수급이 부족해지면서 WHO 마스크 지침과는 상이한 이행을 하였다. 셋째, 한국의 보건당국은 보편적 마스크의 필요성에 따른 마스크 공급 안정화를 기반으로 코로나 19 초기 방역에 성공할 수 있었다. 넷째, WHO 마스크 지침에서 마스크 사용자 제한은 결과론적으로 지침으로서의 부분적 오류를 드러냈다. 분석에서 도출된 함의는 의학적 근거에 따른 국제 지침일지라도 일단 감염병 팬데믹 상황에선 국제 규칙의 엄격한 준수보다 각국의 방역상황 및 역량에 따라 자율적인 지침을 따르도록 허용할 필요가 있다는 점이다.

• 한국산학기술학회논문지
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• 제15권4호
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• pp.2189-2198
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• 2014

본 연구는 제초제 음독 중에서 가장 치명률이 높은 파라쿼트 중독의 독성 감소를 위하여 파라쿼트 독성 기전으로 알려진 파라쿼트 유도성 활성산소종을 감소시키기 위해 기존의 항산화제들 치료제와는 달리, 사전에 산소 농도를 줄여서 파라쿼트에 의한 활성 산소종 발생 자체를 줄이는 저농도 산소요법의 유용성을 연구하였다. 이를 위해 백서를 활용하여 생존률 및 파라쿼트에 의한 활성 산소종의 영향을 가장 빠르고 많이 받는 폐조직의 육안적 조직학적 변화를 비교 분석함으로써 세포 독성 상황에서 저농도 산소요법의 효과를 분석하였다. 그 결과 저농도 산소요법을 처리한 군에서 유의한 생존률 증가와 함께 H&E Stain결과에서는 염증세포 증가 및 Aveolar space에 부종 소견이 저농도 산소요법을 함께 시행한 군에서 완화된 소견을 확인할 수 있었다. 또한 파라쿼트의 독성기전의 핵심인 산화스트레스발생 분석을 시행한 결과, MDA assay, Glutathione assay 및 SOD Assay모두에서 파라쿼트는 산화스트레스를 증가하지만 파라쿼트와 함께 저농도 산소요법을 처리한 경우 산화 스트레스가 감소함을 증명하였다. 이를 통해 본 연구는 임상에서 파라쿼트 중독에 대한 새로운 치료 대안으로서의 기초 연구로서 활용이 기대된다.

• 지적과 국토정보
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• 제51권2호
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• pp.151-168
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• 2021

본 연구는 수도권과 비수도권의 가계지출 부담이 기혼여성의 출산 의사에 미치는 영향을 패널로짓 모형을 이용해 비교분석하였다. 실증분석의 핵심 대상인 기혼여성의 범위는 가임 가능성이 높은 25세부터 39세까지로 한정하였다. 주요 분석 결과는 다음과 같다. 첫째, 거주 지역과는 관계없이 대체로 배우자의 경제력이 기혼여성의 출산 의사에 있어 중요한 요인일 수 있다는 점을 확인하였다. 둘째, 결혼생활 만족도는 높을수록 출산 의사에 긍정적인 영향을 미치고 있었으며, 자녀가 반드시 있어야 한다는 가치관이 있을수록 출산 의사가 높아지는 것으로 나타났다. 셋째, 가계지출 부담은 기존 자녀 수와 같은 내부요인을 제외하고 기혼여성의 출산 의사에 있어 가장 중요한 영향을 미치는 요인임을 확인하였다. 특히 수도권과 비수도권 모두 교육비 지출에 대한 부담은 출산 의사를 큰 폭으로 감소시키는 핵심 원인으로 나타났다. 넷째, 가계지출 부담은 거주 지역에 따라 출산 의사에 상이한 영향을 보이는 것으로 나타났는데, 수도권은 의료비 및 원리금 대출이, 비수도권은 교통통신비가 출산 의사에 더 큰 영향을 미치고 있었다. 이를 통해 본 연구는 출산 제고를 위해서는 가계지출에 대한 부담완화가 지속적으로 필요하며, 거주 지역에 따라 차별적인 정책접근이 요구된다는 시사점을 확인하였다.

• Mass Spectrometry Letters
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• 제8권4호
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• pp.109-113
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• 2017

Single analytical procedure including extraction, liquid chromatography, and mass spectrometric analysis was evaluated for the simultaneous measurement of lysophospholipids (LPLs). LPLs, particularly, lysophosphatidic acids (LPA) and sphingosine 1-phosphate (S1P) are lipid messengers ubiquitously found in various biological matrix. The molecular species mediate important physiological roles in association with many diseases (e.g. cancer, inflammation, and neurodegenerative disease), which emphasize the significance of the simple and reliable analytical method for biomarker discovery and molecular mechanistic understanding. Thus, we developed analytical method mainly focusing on, but not limited by those lipid species S1P and LPA using reverse phase liquid chromatography-tandem mass spectrometry (RPLC-ESI-MS-MS). Extraction method was modified based on Folch method with optimally minimal level of ionization additive (ammonium formate 10 mM and formic acid). Reverse-phase liquid-chromatography was applied for chromatographical separation in combination with negative ionization mode electrospray-coupled Orbitrap mass spectrometry. The method validation was performed on human blood plasma in a non-targeted lipid profiling manner with full-scan MS mode and data-dependent MS/MS. The proposed method presented good inter-assay precision for primary targets, S1P and LPA. Subsequent analysis of other types of LPLs identified a broad range of lysophosphatidylcholines (LPCs) and lysophosphatidyl-ethanolamines (LPEs).

• Molecules and Cells
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• 제43권4호
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• pp.384-396
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• 2020

Breast cancer is one of the most common life-threatening malignancies and the top cause of cancer deaths in women. Although many conventional therapies exist for its treatment, breast cancer still has many handicaps to overcome. Cancer stem cells (CSCs) are a well-known cause of tumor recurrences due to the ability of CSCs for self-renewal and differentiation into cell subpopulations, similar to stem cells. To fully treat breast cancer, a strategy for the treatment of both cancer cells and CSCs is required. However, current strategies for the eradication of CSCs are non-specific and have low efficacy. Therefore, surface biomarkers to selectively treat CSCs need to be developed. Here, 34 out of 641 surface biomarkers on CSCs were identified by proteomic analysis between the human breast adenocarcinoma cell line MCF-7 and MCF-7-derived CSCs. Among them, carcinoembryonic antigen-related cell adhesion molecules 6 (CEACAM6 or CD66c), a member of the CEA family, was selected as a novel biomarker on the CSC surface. This biomarker was then experimentally validated and evaluated for use as a CSC-specific marker. Its biological effects were assessed by treating breast cancer stem cells (BCSCs) with short hairpin (sh)-RNA under oxidative cellular conditions. This study is the first to evaluate the biological function of CD66c as a novel biomarker on the surface of CSCs. This marker is available as a moiety for use in the development of targeted therapeutic agents against CSCs.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3849-3856
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• 2015

Background: Cholangiocarcinoma (CCA), or bile duct cancer, is incurable with a high mortality rate due to a lack of effective early diagnosis and treatment. Identifying cytoplasmic membrane proteins of invasive CCA that facilitate cancer progression would contribute toward the development of novel tumor markers and effective chemotherapy. Materials and Methods: An invasive CCA cell line (KKU-100) was stimulated using TNF-${\alpha}$ and then biotinylated and purified for mass spectrometry analysis. Novel proteins expressed were selected and their mRNAs expression levels were determined by real-time RT-PCR. In addition, the expression of ALCAM was selected for further observation by Western blot analysis, immunofluorescent imaging, and antibody neutralization assay. Results: After comparing the proteomics profile of TNF-${\alpha}$ induced invasive with non-treated control cells, over-expression of seven novel proteins was observed in the cytoplasmic membrane of TNF-${\alpha}$ stimulated CCA cells. Among these, ALCAM is a novel candidate which showed significant higher mRNA- and protein levels. Immunofluorescent assay also supported that ALCAM was expressed on the cell membrane of the cancer, with increasing intensity associated with TNF-${\alpha}$. Conclusions: This study indicated that ALCAM may be a novel protein candidate expressed on cytoplasmic membranes of invasive CCA cells that could be used as a biomarker for development of diagnosis, prognosis, and drug or antibody-based targeted therapies in the future.

• 보건행정학회지
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• 제22권2호
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• pp.263-274
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• 2012

This study describes the hypertensive patients characteristics associated with the health incentive point program, and develops and analyzes a simple predictive model of participation in the program. Based on the Incheon Chronic Disease Management System(iCDMS), a model program of community partnership for hypertensive or diabetic patients detection and follow-up since 2005 in Incheon metropolitan city, a cross-sectional design was used in this study. An effective 10.844 adults sample was divided into groups according to participation in the health incentive point program and continuing treatment, and individual and health characteristics among groups were compared. Furthermore, the predictors associated with participation in the program were identified by the logistic regression analysis. After the health incentive point program in iCDMS was introduced, the number of hypertensive patients participation in the program increased 23.9 times which is vastly high given the various programs were provided. There were statistically significant differences among the groups: age(p=0.000), treatment compliance(p=0.000), and blood pressure control at the last measurement(p=0.000), in particular, between participation group(GroupI, n=246) and non-participation group(GroupIII, n=10,408). Furthermore, age over 60 years(OR: 0.33), treatment compliance(OR: 3.49~3.78) and blood pressure controls(OR: 2.13~2.30) were statistically significant predictors associated with participation in the program, based on the logistic regression analysis with GroupI and GroupIII. To increase participation in the health incentive point program, variables such as age, treatment compliance and blood pressure controls are more concerned. And, high-risk patients and family members need targeted health incentive programs.

• Environmental Analysis Health and Toxicology
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• 제26권
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• pp.10.1-10.7
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• 2011

Objects: Chironomus riparius, a non-biting midge (Chironomidae, Diptera), is extensively used as a model organism in aquatic ecotoxicological studies, and considering the potential of C. riparius larvae as a bio-monitoring species, little is known about its genome sequences. This study reports the results of an Expressed Sequence Tags (ESTs) sequencing project conducted on C. riparius larvae using 454 pyrosequencing. Method: To gain a better understanding of C. riparius transcriptome, we generated ESTs database of C.ripairus using pyrosequencing method. Results: Sequencing runs, using normalized cDNA collections from fourth instar larvae, yielded 20,020 expressed sequence tags, which were assembled into 8,565 contigs and 11,455 singletons. Sequence analysis was performed by BlastX search against the National Center for Biotechnology Information (NCBI) nucleotide (nr) and uniprot protein database. Based on the gene ontology classifications, 24% (E-value${\leq}1^{-5}$) of the sequences had known gene functions, 24% had unknown functions and 52% of sequences did not match any known sequences in the existing database. Sequence comparison revealed 81% of the genes have homologous genes among other insects belonging to the order Diptera providing tools for comparative genome analyses. Targeted searches using these annotations identified genes associated with essential metabolic pathways, signaling pathways, detoxification of toxic metabolites and stress response genes of ecotoxicological interest. Conclusions: The results obtained from this study would eventually make ecotoxicogenomics possible in a truly environmentally relevant species, such as, C. riparius.

• Safety and Health at Work
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• 제15권2호
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• pp.208-212
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• 2024

Background: Traffic accidents involving professional drivers have serious societal repercussions. Unique occupational stressors and health risks exacerbate the likelihood of traffic accidents among professional drivers. This study explores the association between presenteeism-impaired work performance due to working while unwell-and traffic accident risk among professional taxi drivers in Japan. Methods: A prospective cohort study was conducted from June 2022 to February 2023, involving taxi drivers from a single company in Fukuoka Prefecture, Japan. Presenteeism was assessed using the Work Functioning Impairment Scale (WFun). Primary outcome involved the number of self-reported minor traffic accidents. The incidence rate ratio (IRR) of minor traffic accident occurrences was estimated using a Poisson regression analysis, adjusted for confounders including sex, age, and driving experience. Results: Of 838 targeted drivers, 435 were included in the analysis. Higher baseline work functioning impairment was associated with a significant trend of increasing IRR of minor traffic accidents (p for trend = 0.045). A dose-response relationship was seen between the degree of presenteeism and incidence rate of minor traffic accidents. Conclusion: Higher levels of presenteeism were associated with an increased risk of traffic accidents among taxi drivers. The findings underscore the need for socio-economic support and prioritized health management to mitigate traffic accident risk among professional drivers. This study highlights the importance of managing non-critical health issues alongside serious health conditions for safer driving practices among professional drivers in Japan.