• Title/Summary/Keyword: Nitrite reduction

Search Result 141, Processing Time 0.027 seconds

Organic Matter and Hydraulic Loading Effects on Nitrification Performance in Fixed Film Biofilters with Different Filter Media

  • Peng, Lei;Oh, Sung-Yong;Jo, Jae-Yoon
    • Ocean and Polar Research
    • /
    • v.25 no.3
    • /
    • pp.277-286
    • /
    • 2003
  • Nitrification performance of fixed film biofilters using coarse sand, loess bead, or styrofoam beads in biofilter columns 1 meter high and 30cm in diameter were studied at different hydraulic and organic matter loading rates. Synthetic wastewater was supplied to the culture tank in order to maintain desired TAN concentrations in inlet water to biofilters. All the biofilters were conditioned 5 months before start of sampling. TAN and $NO_2-N$ conversion rates increased with an increase in the hydraulic loading rate (HLR). However, the improvement in biofilter performance was not linearly correlated to HLR in styrofoam bead filters. This is mainly due to the characteristics of the styrofoam beads used. TAN conversion rates of sand filters increased with the increase of HLR up to $200m^3/m^2$. per day. No increase in the TAN conversion rate was observed at the highest HLR since flooding on the media surface took place. HLR had a significant impact on the TAN conversion rates in loess bead filter up to the highest HLR tested (P<0.05). TAN conversion rates were much less at organic matter loading rates of 9 and 18kg $O_2/m^3$ per day than those without the addition of organic matter in styrofoam bead filters. The addition of glucose resulted in a reduction of the TAN conversion rate from 540 to 284g $TAN/m^3$ per day. No significant difference of TAN conversion rates between the two organic matter loading rates was found (p<0.05). This indicates that the impact of organic matter on nitrification becomes less and less sensitive with an increase in the COD/TAN ratio. At an organic matter loading rate of 9kg $O_2/m^3$. per day, a great reduction of TAN conversion rates was observed in sand filters and loess bead filters. Clearly, organic matter can be one of the most Important Impacting factors on nitrification. $NO_2-N$ conversion rates showed a similar trend for TAN. Based on the TAN and nitrite conversion rates, styrofoam beads showed the best performance among the three filter media tested. Also, the low gravity and price of styrofoam beads make the handling easier and more cost-effective for commercial application. The results obtained at the highest organic matter loading rates can be used in the biofilter design in recirculating aquaculture system.

The Effect of the Reaction Time Increases of Microbubbles with Catalyst on the Nitrogen Reduction of Livestock Wastewater (가축분뇨의 마이크로버블과 촉매와의 반응 시간 증가에 따라 질소 제거에 미치는 영향)

  • Jang, Jae Kyung;Sung, Je Hoon;Kang, Youn Koo;Kim, Young Hwa
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.37 no.10
    • /
    • pp.578-582
    • /
    • 2015
  • It was investigated whether the removal of nitrogen ions included livestock wastewater were increased by increasing the reaction time of livestock wastewater and microbubbles with catalyst. For this study, the nitrogen reduction system using microbubbles with catalyst was used. The two reactors were consecutively arranged, and the second reactor (Step 2) was located to next the first reactor (Step 1). Each reactor was reacted for 2 hours and air or oxygen as oxidant was fed into the reactor during operation before microbubble device. When oxygen was used, ammonia nitrogen was removed each 18.3% and 52.8% during 2 (only step 1) and 4 (step 1 and step 2) hours reactions. This value was higher than that of when air was fed. When oxygen was used, the longer the reaction time, the ammonia nitrogen removal was higher. The longer the reaction time, the higher the nitrite and nitrate was also removed such as ammonia nitrogen. Also this system was examined whether organic matter removal is effective. The total chemical oxygen demand (TCOD) removal was higher than the soluble chemical oxygen demand (SCOD). Some materials among causing substances COD were difficult to decompose biologically. Therefore, it means that it will be easy to operate the biological processes following step and reduce the concentration of organic contaminants in effluent.

Method for Rapid Determination and Removal of Nitrogen Oxides in Flue Gases (Ⅰ). Rapid Determination on Nitrogen Oxides (배기가스중 질소산화물의 신속측정법과 그 제거에 관한 연구 (제1보). NO$_x$의 신속 정량법)

  • Yong Keun Lee;Tong Oh Seo;Kee Jung Paeng;Man Koo Kim;Kyu Ja Whang
    • Journal of the Korean Chemical Society
    • /
    • v.29 no.1
    • /
    • pp.52-60
    • /
    • 1985
  • Oxides of nitrogen (NO$_x$) in exhaust gases was determined by absorbing the gas in alkaline peroxide solution containing 0.03${\%}$ H2O2 and 0.1N NaOH. About 100 ppm of NO$_x$ was rapidly oxidized to NO$_2$ or N$_2$O$_5$ by H$_2$O$_2$ and required a contact time of 2 minutes with the absorbing solution for complete absorption. With vigorous shaking including air or oxygen gas, high concentration of NO$_x$ (>200 ppm) can be absorbed within 30 minutes. The remaining H$_2$O$_2$ affect the absorbance of color solution strongly. However, the excess H$_2$O$_2$ was completely decomposed by zinc powder 0.5g and the sample solution should be adjusted to the pH range 6.1∼6.6 before the reduction so that conversion of nitrate to nitrite ion is possible. The absorbed NO$_x$ is determined colorimetrically by the diazotization-coupling method with sulfonilamide and NEDA as the coupling agent. The sensitivity of the new method was 4.48 ${\times}$ 10$^4$ as molar absorptivity which was high sensitive compared with that obtained for the usual zinc reduction NEDA method with O$_3$. This method was far more rapid, brief and accurate than previously published O$_3$-NEDA method in Korean industrial standard.

  • PDF

Isolation and Physiological Characteristics of Rhizobia with Good Nitrogen Fixation Ability (우량질소고정균(優良窒素固定菌)의 탐색분리(探索分離) 및 생리적(生理的) 특성(特性))

  • Park, Woo-Churl;Lee, Kwang-Hee;Kim, Jin-Ho;Rhee, In-Koo;Jo, Jin-Ki
    • Current Research on Agriculture and Life Sciences
    • /
    • v.5
    • /
    • pp.40-51
    • /
    • 1987
  • This experiment was conducted to search for Rhizobia with good nitrogen fixation abilities and to investigate their physiological characteristics isolated from 12 soybean cultivars and the affinities of root nodule bacteria with soybeans. The results obtained were as follows; Based on colors, Rhizobia grown on YMA medium were divided into 3 groups, i. e., white, translucent and transparent, amounting to 60, 30 and 10%, respectively. In litmus milk reaction, the strains which produced alkali, acid serum, alkaline serum and acid reached to 51, 29, 9 and 11%, respectively. Strains, S022, and S096 were slow-growers and produced alkaili, while strains, S080, S-090, and S118 were fastgrowers and produced acid. The growth of root nodule bacteria on YMA medium was favorable between the initial pHs of 6.0~7.0. Glutamine, asparagine and al1antoin as nitrogen sources enhanced the growth of root nodule bacteria. All the strains tested formed nodules on the soybean roots, and the strains with good symbiotic nitrogen fixation abilities that had white color, small colony, nitrate reduction abilities and no nitrite reduction abilities showed comparatively high nitrogen fixing activities. Some strains varied in nitrogen fixing activities according to soybean cultivars, and a few strains fanned ineffective nodules which showed no nitrogen fixing activity.

  • PDF

Ecosysteme de I′Etang de Berre (Mediterranee nord-occidentale) : Caracteres Generales Physiques, Chimiques et Biologiques

  • Kim, Ki-Tai
    • Korean Journal of Environmental Biology
    • /
    • v.22 no.2
    • /
    • pp.247-258
    • /
    • 2004
  • Climatological, hydrological and planktonical research studies, measurements of primary production and photosynthetic efficiency from December 1976 to December 1978 have been carried out in two brackish lakes: Lake Etang de Berre and Lake Etang de Vaine located in the French Mediterranean coast, in the region of Carry-le-Rouet located on the north-west Mediterranean near Marseilles, and in fresh water inflows from 4 Rivers (Touloubre, Durance, Arc, Durancole) to Lake Etang de Berre. Physico-chemical parameters were measured for this study: water temperature, salinity, density, pH, alcalinity, dissolved oxygen (% saturation), phosphate, nitrate, nitrite, silicate etc. Diverse biological parameters were also studied: photosynthetic pigments, phaeopigments, specific composition and biomass of phytoplankton, primary pelagic production etc. Climatical factors were studied: air-temperature, solar-radiation, evaporation, direction (including strength) of winds, precipitation and freshwater volume of the four rivers. The changes in Lake ‘Etang de Berre’ ecosystem depend on the quality of the water in the Durance River, and on the effects of seawater near the entrance of the Caronte Canal. The water quality of the lake varies horizontally and vertically as a result of atmospheric phenomena, maritime currents and tides. The distribution of water temperatures is generally heterogeneous. Southeasterly winds and the Northeasterly Mistral wind are important in the origins of circulated and mixed water masses. These winds are both frequent and strong. They have, as a result, a great effect on the water environment of Lake Etang de Berre. In theory, the annual precipitation in this region is well over eight times the water mass of the lake. The water of the Durance River flows into Lake Etang de Berre through the EDF Canal, amounting to 90% of the precipitation. However, reduction of rainfall in dry seasons has a serious effect on the hydrological characteristics of the lake. The temperature in the winter is partially caused by the low temperature of fresh water, particularly that of the Durance River. The hydrological season of fresh and brackish water is about one month ahead of the hydrological season of sea water in its vicinity. The salinity of Lake Etang de Berre runs approximately 3$\textperthousand$, except at lower levels and near the entrance to the Caronte Canal. However, when the volume of the Durance River water is reduced in the summer and fall, the salinity rises to 15$\textperthousand$. In the lake, the ratio of fresh water to sea water is six to one (6:1). The large quantities of seston conveyed by rivers, particularly the Durance diversion, strongly reduce the transparency in the brackish waters. Although the amount of sunshine is also notable, transparency is slight because of the large amount of seston, carried chiefly by Tripton in the fresh water of the Durance River. Therefore, photosynthesis generally occurs only in the surface layer. The transparency progressively increases from freshwater to open seawater, as mineral particles sink to the bottom (about 1.7kg $m^{-2}a^{-1}$ on the average in brackish lakes). The concentration of dissolved oxygen and the rate of oxygen saturation in seawater (Carry-le-Rouet) ranged from 5.0 to 6.0 $m\ell$ㆍ.$1^{-1}$, and from 95 to 105%, respectively. The amount of dissolved oxygen in Etang de Berre oscillated between 2.9 and 268.3%. The monographs of phosphate, nitrate, nitrite and silicate were published as a part of a study on the ecology of phytoplankton in these environments. Horizontal and vertical distributions of these nutriments were studied in detail. The recent diversion of the Durance River into Lake Etang de Berre has effected a fundamental change in this formerly marine environment, which has had a great impact in its plankton populations. A total of 182 taxa were identified, including 111 Bacillariophyceae, 44 Chlorophyceae, and 15 Cyanophyceae. The most abundant species are small freshwater algae, mainly Chlorophyceae. The average density is about $10^{8}$ cells $1^{-1}$ in Lake Etang de Berre, and about double that amount in Lake Etang de Vaine. Differences in phytoplankton abundance and composition at the various stations or at various depths are slight. Cell biovolume V (equivalent to true biomass), plasma volume VP (‘useful’ biomass) and, simultaneously. the cell surface area S and S/V ratio through the measurement of cell dimensions were computed as the parameters of phytoplankton productivity and metabolism. Pigment concentrations are generally very high on account of phytoplankton blooms by Cyanophyceae, Chlorophyceae and Cryptophyceae. On the other hand, in freshwaters and marine waters, pigment concentrations are comparatively low and stable, showing slight annual variation. The variations of ATP concentration were closely related to those of chlorophyll a and phytoplankton blooms only in marine waters. The carbon uptake rates ranged between 38 and 1091 mg$Cm^{-2}d^{-1}$, with an average surface value of 256 mg; water-column carbon-uptake rates ranged between 240 and 2310 mg$Cm^{-2}d^{-1}$, with an average of 810, representing 290 mg$Cm^{-2}$, per year 45 000 tons per year of photosynthetized carbon for the whole lake. Gross photosynthetic production measured by the method of Ryther was studied over a 2-year period. The values obtained from marine water(Carry-le-Rouet) ranged from 23 to 2 337 mg$Cm^{-2}d^{-1}$, with a weighted average of 319, representing about 110 gCm$^{-2}$ per year. The values in brakish water (Etang de Berre) ranged from 14 to 1778 mg$Cm^{-2}d^{-1}$, with a weighted average of 682, representing 250 mg$Cm^{-2}$ per year and 38 400 tons per year of photosynthesized carbon for the whole lake.

Field Assessment of in Situ Remediation of NO3--contaminated Ground Water Using Zero-valent Iron/Bio Composite Media (영가철/바이오 복합처리제를 이용한 질산성 질소 오염 지하수의 현장 지중정화 적용성 평가)

  • Joo, Wan-Ho;Chang, Yoon-Young
    • Journal of Environmental Impact Assessment
    • /
    • v.30 no.1
    • /
    • pp.35-48
    • /
    • 2021
  • In this study, the assessment of field applicability of in-situ remediation of nitrate-contaminated groundwater located in Yesan-gun was performed. Zero-valent iron/bio composite media injected PRB (Permeable Reactive Barrier) and monitoring well were installed in the contaminated groundwater site and monitored main remediation indicators during the PRB operation. Nitrate, nitrite, ammonia, Fe ion, TOC, and turbidity were analyzed and the diversity and population of microorganism in the PRB installed site were investigated for the verification of effect of injected PRB. In the study site where is an agricultural area, a river flows from west to east that forms a river boundary and the southern area has an impermeable sector. It was found that nitrate flows into the river, which is similar as groundwater flow. Simulation result for the fate of nitrate in groundwater showed steady state of nitrate arrived after 3~5 years passed. However, it is just to consider current conditions with no additional input of contaminant source, if additional input of contaminant source occurs contamination dispersion and time for steady state are expected to be increased. The monitoring results showed that Fe ion, TOC and turbidity in groundwater were not clearly changed in concentration after PRB installation, which indicates adaptability of the injected PRB for remediation of groundwater with no additional harmful effect to water quality. The concentration of nitrate maintained less than 5mg/L until 42 days after PRB installation and recovered its initial concentration after 84 days passed and showed termination of reactivity of injected zero-valent iron/bio composite media for removal nitrate. Nitrite and ammonia ions found after installation of PRB indicates reductive removal of nitrate. And the outstanding increase of microorganism diversity and population of Betaproteobacteria Class which includes denitrification microorganism explains biologically reductive removal of nitrate in injected PRB.

Effect of Phytogenic Feed Additives in Soybean Meal on In vitro Swine Fermentation for Odor Reduction and Bacterial Community Comparison

  • Alam, M.J.;Mamuad, L.L.;Kim, S.H.;Jeong, C.D.;Sung, H.G.;Cho, S.B.;Jeon, C.O.;Lee, K.;Lee, Sang Suk
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.26 no.2
    • /
    • pp.266-274
    • /
    • 2013
  • The effect of different phytogenic feed additives on reducing odorous compounds in swine was investigated using in vitro fermentation and analyzed their microbial communities. Soybean meal (1%) added with 0.1% different phytogenic feed additives (FA) were in vitro fermented using swine fecal slurries and anaerobically incubated for 12 and 24 h. The phytogenic FAs used were red ginseng barn powder (Panax ginseng C. A. Meyer, FA1), persimmon leaf powder (Diospyros virginiana L., FA2), ginkgo leaf powder (Ginkgo biloba L., FA3), and oregano lippia seed oil extract (Lippia graveolens Kunth, OL, FA4). Total gas production, pH, ammonianitrogen ($NH_3$-N), hydrogen sulfide ($H_2S$), nitrite-nitrogen ($NO_2{^-}$-N), nitrate-nitrogen ($NO_3{^-}$-N), sulfate (${SO_4}^{--}$), volatile fatty acids (VFA) and other metabolites concentration were determined. Microbial communities were also analyzed using 16S rRNA DGGE. Results showed that the pH values on all treatments increased as incubation time became longer except for FA4 where it decreased. Moreover, FA4 incubated for 12 and 24 h was not detected in $NH_3$-N and $H_2S$. Addition of FAs decreased (p<0.05) propionate production but increased (p<0.05) the total VFA production. Ten 16S rRNA DGGE bands were identified which ranged from 96 to 100% identity which were mostly isolated from the intestine. Similarity index showed three clearly different clusters: I (FA2 and FA3), II (Con and FA1), and III (FA4). Dominant bands which were identified closest to Eubacterium limosum (ATCC 8486T), Uncultured bacterium clone PF6641 and Streptococcus lutetiensis (CIP 106849T) were present only in the FA4 treatment group and were not found in other groups. FA4 had a different bacterial diversity compared to control and other treatments and thus explains having lowest odorous compounds. Addition of FA4 to an enriched protein feed source for growing swine may effectively reduce odorous compounds which are typically associated with swine production.

Effects of Heat Treatments on Physicochemical Properties and In Vitro Biological Activities of Quinoa (Chenopodium quinoa Willd.) (퀴노아의 열처리 가공에 따른 이화학적 특성 및 In Vitro 생리활성)

  • Goh, Hye-Kyung;Lee, Young-Tack
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.46 no.6
    • /
    • pp.688-694
    • /
    • 2017
  • The effects of heat treatments on the physicochemical properties and in vitro biological activities of quinoa (Chenopodium quinoa Willd.) were investigated. Quinoa grains were subjected to two different heat treatment methods: boiling and steaming plus roasting (steaming/roasting). Compared with raw quinoa, boiled quinoa samples had slightly lower crude protein, crude fat, crude ash, and starch contents. However, steaming/roasting treatment did not cause significant differences in proximate composition. Heat treatments reduced total phenolic and flavonoid contents in quinoa extracts, and higher reduction was detected upon boiling treatment. Heat treatments also reduced lightness and increased yellowness of quinoa samples. Heat treatments increased water absorption index but decreased water solubility index. In vitro starch hydrolysis increased substantially after both heat treatments, and slightly higher values were observed in the boiled quinoa samples. 1,1-Diphenyl-2-picrylhydrazyl free radical scavenging activity and nitrite scavenging activity were reduced by heat treatments, and the boiled quinoa sample showed the lowest activity likely due to loss of activities in cooking water.

Estimation of verticle fluxes of nitrogen compounds in tidal flats of the Keum river estuary (금강하구 갯벌내 질소화합물질의 연직적인 플럭스 평가)

  • Kim Do Hee;Yang Jae Sam
    • Journal of the Korean Society for Marine Environment & Energy
    • /
    • v.3 no.2
    • /
    • pp.3-10
    • /
    • 2000
  • The main purpose of this study were to estimate the benthic fluxes of dissolved inorganic nitrogen (DIN) from the sediment and denitrification rates in tidal flats of the Keum river estuary. Sediment specimens were collected by a core sampler from three stations along the Keum river estuary in April, August and December, 1999. The sediments were composed of 1.18 %, 29.34 % and 69.49 % of gravel and sand, sand and silt, respectively. The mean ignition loss of the sediment was found 6.7 % and its Oxidation Reduction Potential (ORP) was measured -12 mV. The total hydrogen sulfides was determined about 0.26 mg/gㆍdry. The estimated outflux of ammonium was found 11.2 m mole N/m²ㆍday from the sediment, whereas -1.09 m mole N/m²ㆍday of influx was obtained for nitrate and nitrite through the incubation experiment of sediment cores. Total DIN flux was 10.2 m mole N/m²ㆍday outflux from the sediment. From the incubation experiments executed with the flux studies, mean denitrification rate was found 30.6 m mole N₂/m²ㆍday measured by the direct assay of N₂ production technique. On the basis that DIN flux and denitrification rate in sediment of tidal flat of the Keum river estuary are may be effects to control the algal biomass in the coastal environment, it seems inevitable to pay more attention to investigate the flux of DIN and denitrification rate in tidal flat of the Keum river estuary.

  • PDF

Effect of Nitrogen Compounds and Organic Carbon Concentrations on $N_2O$ Emission during Denitrification (탈질에서 질소성분 및 유기탄소 농도가 $N_2O$ 배출에 미치는 영향)

  • Kim, Dong-Jin;Kim, Heon-Ki;Kim, Yu-Ri
    • Clean Technology
    • /
    • v.17 no.2
    • /
    • pp.134-141
    • /
    • 2011
  • The effects of the compounds and concentrations of nitrogenous electron acceptor, the ratio of electron donor/electron acceptor (C/N), and the complexity of electron donor on the emission of $N_2O$ during wastewater denitrification were quantitatively investigated in this study. The higher ${NO_3}^-$ and ${NO_2}^-$ concentrations, the more $N_2O$ emission was observed. ${NO_2}^-$ has strong effect on $N_2O$ emission as it emitted morc $N_2O$ than ${NO_3}^-$, 50 mg/L of ${NO_2}^-$-N gave the highest conversion (9.3%) and yield (9.8%) of $N_2O$ while ${NO_3}^-$-N (50 mg/L) gave 5.6% conversion and 11.0% yield. Lower C/N ratio decreases nitrogen removal efficiency, but it increases the conversion of $N_2O$ because of the incomplete denitrification by the limited organic carbon. When real domestic wastewater is used as the electron donor of the denitrification, $N_2O$ emission is reduced to 1/10 of the emission when single carbon (acetate) is used. It is thought that multiple carbon source utilizes many denitrification pathways and it seems to be helpful for the reduction of $N_2O$ emission.