• 한국작물학회지
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• 제61권1호
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• pp.64-69
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• 2016

This study was conducted to develop a fast and efficient screening method to determine the quantity of fatty acid in peanut oil for high oleate breeding program. A total of 329 peanut samples were used in this study, 227 of which were considered in the calibration equation development and 102 were utilized for validation, using near infrared reflectance spectroscopy (NIRS). The NIRS equations for all the seven fatty acids had low standard error of calibration (SEC) values, while high R2 values of 0.983 and 0.991 were obtained for oleic and linoleic acids, respectively in the calibration equation. Furthermore, the predicted means of the two main fatty acids in the calibration equation were very similar to the means based on gas chromatography (GC) analysis, ranging from 36.7 to 77.1% for oleic acid and 7.1 to 42.7% for linoleic acid. Based on the standard error of prediction (SEP), bias values, and $R^2$ statistics, the NIRS fatty acid equations were accurately predicted the concentrations of oleic and linoleic acids of the validation sample set. These results suggest that NIRS equations of oleic and linoleic acid can be used as a rapid mass screening method for fatty acid content analysis in peanut breeding program.

• 미생물학회지
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• 제46권3호
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• pp.255-261
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• 2010

남태평양에 서식하는 두 종의 해면 Hyrtios sp. 604와 Callyspongia sp. 612로부터 16S rDNA DGGE 방법을 이용하여 공생세균 군집의 다양성을 분석하였다. DGGE band로부터 염기서열 분석 결과, Hytrios sp. 604의 공생세균은 Alphaproteobacteria, Gammaproteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Cyanobacteria, Firmicutes로 나타났으며, Callyspongia sp. 612의 공생세균 그룹은 Alphaproteobacteria, Gammaproteobacteria, Chloroflexi, Cyanobacteria로 나타났다. 풍부한 이차대사산물의 생산자로 보고된 Hyrtios 해면 속의 Hyrtios sp. 604는 Callyspongia sp. 612에 비해 더 다양한 공생세균 군집을 나타내었으며 주요 공생세균 군집으로 Actionobacteria가 포함되었다. 동일 지역에 서식하나 화학적 특성이 다른 두 해면 종의 세균 군집은 서로 다른 것으로 나타났다. 발견된 공생세균의 염기서열은 90% 이상이 uncultured clone들과 높은 상동성을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.453-464
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• 2014

The current research was focused on the extracellular biosynthesis of bactericidal silver nanoparticles (AgNPs) using cell-free supernatant of a local isolate previously identified as a novel Streptomyces aegyptia NEAE 102. The biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102 was quite fast and required far less time than previously published strains. The produced particles showed a single surface plasmon resonance peak at 400 nm by UV-Vis spectroscopy, which confirmed the presence of AgNPs. Response surface methodology was chosen to evaluate the effects of four process variables ($AgNO_3$ concentration, incubation period, pH levels, and inoculum size) on the biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102. Statistical analysis of the results showed that the linear and quadratic effects of incubation period, initial pH, and inoculum size had a significant effect (p < 0.05) on the biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102. The maximum silver nanoparticles biosynthesis (2.5 OD, at 400 nm ) was achieved in runs number 5 and 14 under the conditions of 1 mM $AgNO_3$ (1-1.5% (v/v)), incubation period (72-96 h), initial pH (9-10), and inoculum size (2-4% (v/v)). An overall 4-fold increase in AgNPs biosynthesis was obtained as compared with that of unoptimized conditions. The biosynthesized silver nanoparticles were characterized using UV-VIS spectrophotometer and Fourier transform infrared spectroscopy analysis, in addition to antimicrobial properties. The biosynthesized AgNPs significantly inhibited the growth of medically important pathogenic gram-positive (Staphylococcus aureus) and gram-negative bacteria (Pseudomonas aeruginosa) and yeast (Candida albicans).

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1282-1292
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• 2018

The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, and DEAE-cellulose and Sepharose CL-6B chromatographies. The molecular mass of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be ${\alpha}-glucan$ that consisted of a trisaccharide repeating unit with possible presence of two ${\alpha}-(1{\rightarrow}3)$ and one ${\alpha}-(1{\rightarrow}6)$ glucosidic linkages. Microstructural analysis showed that the EPS appeared as ellipsoid or globose with a smooth surface. The EPS had a degradation temperature at $240^{\circ}C$. Furthermore, the EPS had strong DPPH and hydroxyl radical scavenging activities, and moderate superoxidant anion scavenging and metal ion-chelating activities. This is the first characterization of a glucan produced by B. amyloliquefaciens with strong antioxidant activity. The results of this study suggest the potential of the EPS from B. amyloliquefaciens GSBa-1 to serve as a natural antioxidant for application in functional products.

• ALGAE
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• 제32권1호
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• pp.75-86
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• 2017

Polysaccharides of marine algae exhibit different structural characteristics and interesting biological functions. In this study, crude polysaccharides (CP) of eleven Sri Lankan marine algae obtained through hot water extraction and ethanol precipitation were investigated for DPPH, alkyl, and hydroxyl radical scavenging activities using electron spin resonance spectrometry and for intracellular reactive oxygen species scavenging activity in the Chang liver cell line. Characterization of CPs was done by Fourier transform infrared (FTIR) spectroscopy and by analysis of the monosaccharide composition. Time-dependent density functional theory quantum-chemical calculations at the RB3LYP/6-31G(d,p) level for constructed dimeric units of the corresponding polysaccharides were used to resolve the FTIR spectra. CPs from Chnoospora minima showed the highest DPPH and alkyl radical scavenging activities and higher intracellular reactive oxygen species scavenging effects for both AAPH and $H_2O_2$ induced ROS production in "Chang" cells. The major polysaccharide constituent in C. minima CP was identified as fucoidan and it displayed a higher sulfate content. The degree of sulfation of these polysaccharides suggests a positive correlation with the observed antioxidant properties.

• 한국포장학회지
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• 제27권1호
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• pp.9-16
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• 2021

The present study was conducted to accomplish management efficiency by preparing preemptive measures for consumer dissatisfaction and overcome risks caused by Global Warming through studies of model cases in packaging. Through this study, we made it possible to find a way for companies to prepare for Global warming and consumer dissatisfaction. By contributing to eco-friendly packaging, we are trying to preemptively respond to recent major issues through packaging. Through this experiment, we tried to measure the degree of penetration of Plodia interpunctella H. larva into the insect repellent film produced by printing Ink containing a natural repellent. The control experiment was conducted with an untreated LDPE film to which no insect repellent was applied. The numbers decreased from 17 to 7 when the film was processed with repellent to show experimental results verifying effects of repellent by decrease in 10 (58.8% Decrease). Such results show that it is safe when the film is unfolded but in the case where the film is folded, the Plodia interpunctella H. punches through the film to lead to consumer dissatisfaction and it suggests that this can bring on risks to corporate management. Considering that most of the film is folded in the case of PE bag packaging, the direction which the corporations should take in terms of preparing for climate change countermeasures and consumer dissatisfaction has been clarified. Due to it receiving satisfactory results in safety rest results for printing film applied with pest repellent as well as the Quality analysis to test repellent contents of repellent film, it is certain that the importance of repellent method in packaging will increase in preparing for consumer dissatisfaction and actions against climate change henceforth.

• 인터넷정보학회논문지
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• 제20권1호
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• pp.57-65
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• 2019

200 만명 이상의 회원을 보유하고 있는 "도매꾹" B2B 온라인 쇼핑몰인 경우70% 이상의 시장 점유율로 하루에 80만개 이상의 아이템이 판매되고 있다. 하지만, 동일하거나 유사한 물품이 서로 다른 카탈로그 엔트리에 저장 및 등록되어 있기 때문에 구매자가 아이템을 검색하는 과정에서 어려움을 느끼며 B2B 대형 쇼핑몰 관리에도 문제점이 발생하고 있다. 따라서 이에 대한 해결 방안으로 본 연구에서는 대단위 쇼핑몰 구매 정보를 기반으로 지도-학습 머신러닝 기법을 적용하여 상품에 대한 카탈로그 목록 자동 분류 및 추천 시스템을 개발하였다. 구체적으로 판매자가 자연어 형태로 물품 등록 정보를 입력하면 KoNLPy 형태소 분석 과정을 수행하였으며, Naïve Bayes 분류 방식을 응용하여 물품에 가장 적합한 카탈로그 정보를 자동으로 추천해주는 시스템을 구현하였다. 정확도가 향상된 카테고리 목록을 구축하여 결과적으로 검색 속도와 쇼핑몰 매출을 향상시키는 효과가 있었다.

• 한국물환경학회지
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• 제34권6호
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• pp.661-668
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• 2018

Geosmin is volatile metabolites produced by a range of filamentous cyanobacteria which causes taste and odor problems in drinking water. Molecular ecological methods which target biosynthetic genes (geoA) are widely adopted to detect geosmin-producing cyanobacteria. The aim of this study was to investigate the potential production capability of 8 strains isolated from the Nakdong River. Ultimately, a suggestion for a genetical monitoring tool for the identification of geosmin producers in domestic waters was to be made. Geosmin was detected using solid phase microextraction gas chromatography mass spectrometry (SPME GC-MS) in two strains of Dolichospermum plactonicum (DGUC006, DGUC012) that were cultured for 28 day. The highest concentrations during the experiment period was $17,535ngL^{-1}$ and $14,311ngL^{-1}$ respectively. Additionally, geoA genes were amplified using two primers (geo78F/971R and geo78F/982R) from strains shown to produce geosmin, while amplification products were not detected in any of non-producing strains. PCR product (766 bp) was slightly shorter than the expected size for geosmin producers. According to the BLAST analysis, amplified genes were at nucleotide level with Anabaena ucrainica (HQ404996, HQ404997), Dolichospermum planctonicum (KM13400) and Dolichospermum ucrainicum (MF996872) between 99 ~ 100 %. Both strains were thus confirmed as potential geosmin-producing species. We concluded that the molecular method of analysis was a useful tool for monitoring potential cyanobacterial producers of geosmin.

• 농업과학연구
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• 제49권3호
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• pp.547-560
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• 2022

The present study investigated the effects of dietary supplementation of peanut shell extract on the growth performance and physiological properties of broiler chicks. Two diet energy levels (Positive and Negative) and four additives (0.0, 0.05, and 0.1% peanut shell extract and commercial antioxidant) were factorially arranged for eight treatments. The overall weight gain of the broilers was slightly improved at 0.05% for the antioxidant treatments regardless of the diet energy levels, but there was no statistical difference among the treatments (p > 0.05). The carcass characteristics of the broilers, such as cooking loss, crude protein content, antioxidant activity, and thiobarbituric acid reactive substances (TBARS) values, were improved by the feeding diets containing the 0.05% peanut shell extract. Furthermore, it was confirmed that the dietary supplementation of peanut shell extract did not have a negative effect on the immune responses of the broilers show by the lack of statistical differences in the liver and bursa Fabricious weight and cytokine level among the treatments. From the economic analysis, dietary supplementation of peanut shell extract significantly influenced the compensatory growth and food efficiency and, in turn, led to a decrease in the duration needed to reach 1.5 kg compared to the control. These results suggest the possibility that the peanut shell extract could be used as a functional feed additive by improving the growth performance and carcass characteristics with no detrimental effects on broilers.

• 융합정보논문지
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• 제12권3호
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• pp.1-10
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• 2022

배추와 같은 채소류는 자연재해의 영향을 많이 받기 때문에 폭우나 병해와 같은 재해로 인해 가격 변동이 심해져 농가 경제에 영향을 미치게 된다. 이러한 문제를 해결하기 위해서 농산물 가격 예측을 위한 다양한 노력이 행해졌지만 극심한 가격 예측 변동을 예측하기는 어렵다. 본 연구에서는 단일 분류기를 결합하여 다양한 여러 개의 분류기를 통해 최종 예측 결과를 결정하는 방식인 앙상블 Voting 기법으로 배추 가격을 분석하였다. 또한 시계 열 분석 방법인 LSTM과 부스팅 기법인 XGBoost와 RandomForest로 결과 비교를 하였다. 가격 데이터는 일별 데이터를 사용하였고 배추 가격에 영향을 주는 기상정보와 물가지수 등을 사용하였다. 연구 결과로는 실제값과 예측값의 차이를 보여주는 RMSE 값이 약 236 수준이다. 이 연구를 활용하여 농산물 가격 예측과 같은 다른 시계 열 분석 연구 모델 선정에 활용할 수 있을 것으로 기대된다.