Fig. 1. Photographs of the strains isolated in Nakdong River. Akinetes and heterocytes are indicated with white and black arrows respectively. (a) Dolichospermum planctonicum, (b) D. crassum, (c) Aphanizomenon flos-aquae, (d) Planktothrix agardhii, (e) Geitlerinema amphibium, (f) Pseudanabaena westianam, (g) P. mucicola.
Fig. 2. Gene cluster responsible for the synthesis of geosmin in two strains of D. ucrainicum. Upper: strain CHAB 1432; Lower: strain CHAB 2155 (after Wang et al., 2015).
Fig. 3. Maximum likelihood tree based on the sequences of putative geosmin synthase from cyanobacteria, proteobacteria and actinobacteria. The strains of study are marked by shading. Numbers at the nodes are levels of bootstrap support. Scale bar indicates 5% sequence divergence. Cluster I and Cluster II denote Order Oscillatoriales and Order Nostocales, respectively.
Fig. 4. Changes of cell growth and geosmin production in Dolichospermum plactonicum (strain no.: DGUC006) strain culture. The curve graph represents a mean value of cell densities and vertical bars represent standard deviation. The bar graphs represent a mean value of geosmin concentraion.
Table 1. Information on strains isolated in the Nakdong River
Table 2. List of primer pairs used for PCR and sequencing of geoA synthase gene.
Table 3. Blast analysis using the geoA gene. The library with the highest similarity was presented by the GenBank (National Center for Biotechnology Information, USA).
Table 4. Strains used in this study for PCR and sequencing geoA, detecting geosmin. PCR was performed with primer pair geo78F/971R and geo78F/982R.
References
- Acinas, S. G., Haverkamp, T. H., Huisman, J., and Stal, L. J. (2009). Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria), The ISME Journal, 3, 31-46. https://doi.org/10.1038/ismej.2008.78
- Byun J. H., Hwang, S. J., Kim, B. H., Park, J. R., Lee, J. K., and Lim, B. J. (2015). Relationship between a dense population of cyanobacteria and odorous compounds in the Han river system in 2014 and 2015, Journal of Korean Society on Water Environment, 48, 263-271. [Korean Literature]
- Chae, A. N., Shin, J. W., Jo, K. W., Lee B. C., and Song, K. G. (2017). Removal of geosmin and 2-methylisoborneol in dringking water by powdered activated carbon, Journal of the Korean Society of Civil Engineers, 37, 475-483. [Korean Literature]
- Dickschat, J. S., Bode, H. B., Mahmud, T., Müller R., and Schulz, S. (2005) A novel type of geosmin biosynthesis in myxobacteria, The Journal of Organic Chemistry, 70, 5174-5182.
- Dickschat, J. S., Thorben, N., Thiel, V., Kunze, B., Müller, R., and Schulz, S. (2007). Biosynthesis of the off-flavor 2-methylisoborneol by the myxobacterium Nannocystis exedens, Angewandte Chemie International Edition, 46, 8287-8290.
- Giglio, S., Jiang, J., Saint, C. P., Cane D. E., and Monis, P. T. (2008). Isolation and characterization of the gene associated with geosmin production in cyanobacteria, Environmental Science and Technology, 42, 8027-8032.
- Izaguirre, G. and Taylor, W. D. (2004). A guide to geosminand MIB-producing cyanobacteria in the United States, Water Science and Technology, 49, 19-24.
- Juttner, F. and Watson, S. B. (2007). Biochemical and ecological control of geosmin and 2-methylisoborneol in source waters, Applied and Environmental Microbiology, 73, 4395-4406.
- Kakimoto, M., Ishikawa, T., Miyagi, A., Saito, K., Miyazaki, M., Asaeda, T., Tamaguchi, M., Uchimiya, H., and Kawai-Yamada, M. (2014). Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata, Jouurnal of Plant Physiology, 171, 292-300.
- Kim, K. H., Lim, B. J., You, K. A., Park, M. H., Park, J. H., Kim, B. H., and Hwang, S. J. (2014). Identification and analysis of geosmin production potential of Anabaena strain isolated from North Han river using genetic methods, Korean Journal of Ecology and Environment 47, 342-349. [Korean Literature]
- Kim, Y. J., Youn, S. J., Kim, H. N., Hwang, M. Y., Park, J. R., Lee, B. C., and Lee, J. K. (2015). Formation of phytoplankton community and occurences of odorous compounds for sediment incubation by water temperature, Journal of Korean Society on Water Environment, 31, 460-467. [Korean Literature]
- Komarek, J. (2013). Cyanoprokaryota 3. Teil / 3rd part: Heterocytous Genera, In: B. Bubel, G. Gartner, L. Krienitz and M. Schager l (eds.), SuBwasser flora von Mutteleuropa, 19/3, Springer Spektrum, 1131.
- Komarek, J. and Anagnostidis, K. (2005). Cyanoprokaryota 2. Teil/2nd Part: Oscillatoriales, In: Budel, B., Krienitz, L., Gartner, G. and M. Schagerl, (eds.) SuBwasserflora von Mutteleuropa, 19/2, Elsevier/Spektrum, Heidelberg, 759.
- Kutovaya, O. A. and Watson, S. B. (2014). Development and application of a molecular assay to detect and monitor geosmin-producing cyanobacteria and actinomycetes in the great lakes, Journal of Great Lakes Research, 40, 404-414.
- Ministry of Environment (ME). (2011). Standard method for water pollutants, Ministry of Environment. [Korean Literature]
- Shirai, M., Matumaru, K., Ohotake, A., Takamura, Y., Aida, T., and Nakano, M. (1989). Development of a solid medium for growth and isolation of axenic Microcystis strains (Cyanobacteria), Applied and Environmental Microbiology, 55, 2569-2571.
- Li, X., Dreher, T. W., and Li, R. (2016). An overview of diversity, occurence, genetics and toxin production of bloom-forming Dolichospermum(Anabaena) species, Harmful algae, 54, 54-68. https://doi.org/10.1016/j.hal.2015.10.015
- Park, H. J., Park, M. H., Shim, Y. B., Im, J. K., and Hwang, S. J. (2017). Geosmin production potential of cyanobacterium, Anabaena circinalis isolated from lake Paldang, Korea, Korean Journal of Ecology and Environment 50, 363-373. [Korean Literature]
- Ryu, H. S. (2017). Taxonomy and toxigenecity of bloom-forming Aphanizomenon spp. and related species within the nostocales (Cyanophyceae), Doctor's Thesis, Daegu University, Gyungsan, South Korea, 121. [Korean Literature]
- Ryu, H. S., Shin, R. Y., and Lee, J. H. (2017). Morphology and Taxonomy of the Aphanizomenon spp. (Cyanophyceae) and related species in the Nakdong river, South Korea, Journal of Ecology and Environment, 41(6). https://doi.org/10.1186/s41610-016-0021-0
- Ryu, H. S., Shin, R. Y., Seo, K. A., Lee, J. H., and Kim, K. H. (2018). Succession of cyanobacterial species and taxonmical characteristics of Dolichospermum spp. (Nostocales, Cyanophyceae) in weir region of the Nakdong river, Journal of Korean Society on Water Environment, 34, 503-513. [Korean Literature]
- Su, M., Gaget, V., Giglio, S., Burch, M., An, W., and Yang, M. (2013). Establishment of quantitative PCR methods for the quantification of geosmin producing potential and Anabaena sp. in freshwater systems, Water Research, 47, 3444-3454.
- Suurnakki S., Gomez-Saez, G. V., Rantala-Ylinen, A., Jokela, J., Fewer, D. P., and Sivonen, K. (2015). Identification of geosmin and 2-methylidoborneol in cyanobacteria and molecular detection methods for the producers of these compounds, Water Research, 68, 56-66.
- Tamura, K. Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA 5: Meolecular Evolutionary Genetics Analysis, Arizona: Center for Evolutionary Functional Genomics The Biodesign Institute.
- Wang Z., Shao, J., Xu, Y., Yan, B., and Li, R. (2015). Genetic basis for geosmin production by the water bloom-forming cyanobacterium, Anabaena ucrainica, Water, 7, 175-187.
- Watson, S. B., Ridal, J., and Boyer, G. L. (2008). Taste and odour and cyanobacterial toxins: impairment, prediction, and management in the great lakes, Canadian Journal of Fisheries and Aquatic Sciences, 65, 1779-1796.
- You, K. A., Byeon, M. S., Youn, S. J., Hwang, S. J., and Rhew, D. H. (2013). Growth characteristics of blue-green algae (Anabaena spiroides) causing tastes and odors in the North-Han river, Korea, Korean Journal of Ecology and Environment 46, 135-144. [Korean Literature]
- Zhang T, Li, L., Song, L., and Chen, W. (2009) Effects of temperature and light on the growth and geosmin production of Lyngbya kuetzingii (Cyanophyta), Journal of Applied Phycology, 21, 279-85.