• Title/Summary/Keyword: Natural Standard

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Antioxidant activities of Erythrina stricta Roxb.using various in vitro and ex vivo models

  • AsokKumar, K;UmaMaheswari, M;Sivashanmugam, AT;SubhadraDevi, V;Subhashini, N;Ravi, TK
    • Advances in Traditional Medicine
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    • v.8 no.3
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    • pp.266-278
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    • 2008
  • Erythrina stricta, a deciduous tree widely used traditionally in indigenous system of medicine for various ailments such as rheumatism, fever, leprosy, epilepsy etc. The leaves of Erythrina stricta was extracted with ethanol (70%) and used for the evaluation of various in vitro antioxidant assays which includes H - donor activity, nitric oxide scavenging, superoxide anion scavenging, reducing ability, hydroxyl radical, hydrogen peroxide scavenging, total phenolic content, total flavonoid content, total antioxidant activity by thiocyanate and phosphomolybdenum method, metal chelating, $\beta$-carotene bleaching, total peroxy radical assays. The pro-oxidant activity was measured using bleomycin-dependent DNA damage. Ex vivo models like lipid peroxidation and erythrocyte haemolysis were also used to study the antioxidant property of the extract. The various antioxidant activities were compared with suitable standard antioxidants such as ascorbic acid, butylated hydroxyl toluene, $\alpha$-tocopherol, curcumin, quercetin and Trolox. The generation of free radicals viz. $O_2^{{\cdot}-}$, $OH^{\cdot}$, $H_2O_2$, $NO^{\cdot}$ and peroxyl radicals were effectively scavenged by the ethanolic extract of Erythrina stricta. In all the methods, the extract offered strong antioxidant activity in a concentration dependent manner. The total phenolic content, flavonoid content and total antioxidant activity in Erythrina stricta were determined as microgram (g) pyrocatechol, quercetin and $\alpha$-tocopherol equivalent/mg respectively. The extract did not exhibit any prooxidant activity when compared with ascorbic acid. The results obtained in the present study clearly indicates that Erythrina stricta scavenges free radicals and reduces lipid peroxidation, ameliorating the damage imposed by oxidative stress in different disease conditions and serve as a potential source of natural antioxidant.

Effect of plate size on meal energy intake in normal weight women

  • Ayaz, Aylin;Akyol, Asli;Cetin, Cansu;Besler, H. Tanju
    • Nutrition Research and Practice
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    • v.10 no.5
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    • pp.524-529
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    • 2016
  • BACKGROUND/OBJECTIVES: Use of smaller plates to control food intake is a commonly recommended strategy for restricting energy intake, despite conflicting results. Therefore, the aim of this study was to examine whether or not three different sizes of plates influence energy intake during a multi-itemed buffet meal in normal weight women. SUBJECTS/METHODS: This was a cross-over study conducted on 37 female participants aged 19-25 years with normal BMI levels. Participants were recruited from Hacettepe University and the surrounding community. On experimental days, participants ate a standard breakfast and were then randomly assigned to eat lunch using a small (19 cm), medium (23 cm), or large (28 cm) diameter plate. Visual analogue scale (VAS) scores on sensory and satiety outcomes were measured for all meals. Energy and macronutrient intakes during lunch were recorded. RESULTS: There was no evidence that use of a smaller plate size reduced energy or specific macronutrient intake during the free choice lunch meal. Multiple visits to the serving table were not associated with energy or macronutrient intake. Plate size did not affect VAS scores during the test days. CONCLUSIONS: Plate size did not influence energy intake, meal composition, or palatability in normal weight women during a multi-itemed open buffet lunch. Studies in natural settings at the population level are needed to clarify current outcomes.

Rice Growth Response and Soil Quality by Domestic Wastewater Irrigation on Rice Paddy Field - Lysimeter experiment - (하수종말처리장 방류수를 논 관개용수로 처리시 벼 생육 및 토양환경 영향 조사 - 라이시미터 재배실험 -)

  • Cho, Jae-Young;Park, Seung-Woo;Son, Jae-Gwon;Park, Bong-Ju;Li, Long-Gen
    • Journal of Korean Society of Rural Planning
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    • v.12 no.2 s.31
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    • pp.49-56
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    • 2006
  • The application of domestic wastewater on rice paddies results in the accumulation of sodium(Na$^+$) to the soil. Excessive concentration of sodium may cause the deterioration of the physical characteristics of the soil, change in the osmosis of the soil, destruction of soil aggregates as well as ion toxicity due to sodium accumulation. Using domestic wastewater as irrigation water should be preceded by measures to prevent or control the soil salinization caused by sodium. Agricultural reuse of domestic wastewater were found not to cause serious problems with food safety due to heavy metals. However, pre-treatment using ultraviolet or ozone is recommended to reduce the number of bacteria and gem and for public health reasons. Using domestic wastewater has shown that reducing the standard application of chemical fertilizers by as much as 50% reduced the harvesting index by only 10%. This study has shown that it is feasible to reuse domestic wastewater on rice paddies. In order to facilitate the application, it is deemed necessary to establish wastewater treatment technologies in the future, to review criteria for recycling domestic wastewater for agricultural purposes such as conditions of soil and cropping system and to resolve conflicts with farmers and public health issues.

A Study on User Satisfaction of Information Facilities for the Visually Impaired (시각장애인의 실내안내시설 설치 이용만족도에 관한 연구)

  • Kim, In-Bae;Kim, Won-Pil
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.26 no.2
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    • pp.19-30
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    • 2020
  • Purpose: The ratio of the visually impaired is gradually increasing due to various causes such as natural aging, accidents, and environmental changes. Therefore, this study has two folds. Firstly, it aims to analyze the current satisfaction level with information facilities for the visually impaired. Secondly, it aims to examine the factors affecting the satisfaction with indoor information facilities. Through this study, it helps to identify problems of information facilities for the visually impaired and it works as a practical data for further improvements. Methods: For 50 visually impaired people, a telephone survey was conducted on the satisfaction with the braille blocks, braille signboards, braille information boards with tactile map, audio guide devices, and safety egress devices. Microsoft Excel and SPSS 26.0 were used for the analysis. Results: It was confirmed that the overall satisfaction with the indoor information facilities was very low. The more the visually impaired people recognized the installation standards, and the better the maintenance was, the higher the satisfaction. In the case of braille blocks and braille signboards, the effect of information transfer on satisfaction was relatively high. In the case of audio guide device and safety egress device, the standard suitability influenced satisfaction. Implications: The low level of satisfaction with the overall information facilities can impede visually impaired people from receiving practical help through the information facilities. Factors influencing the satisfaction level of each facility were identified, which makes it possible to prepare effective improvement measures.

The Analysis of the Educational Conditions of Interior Design in Korea - Focused on the Curriculum of 4 year Universities (한국 실내디자인 분야의 교육현황 분석 - 4년제 대학교의 교과과정을 중심으로 -)

  • 신경주
    • Korean Institute of Interior Design Journal
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    • no.38
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    • pp.3-13
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    • 2003
  • Now that 15 years have passed since its introduction to 4 year college in Korea and we have 51 majors in the field of Intoner design established at 47 universities as of 2003, we are in the position to seek the improvement of its educational qualify. With a view to provide data for the setup of proper educational purpose, it has classified their divisions(departments) according to their major names and belonged colleges of the universities and considered their geographical distributions, groupings of curriculum, their framing and features of a standard curriculum. 1)Divisions of the universities where the major of interior design belongs are divided into three categories of design division (design 8t arts, arts), home economics division (home economics, natural science) and architectural division. For the development of the major, universities to which circumstances permit are recommended to establish an independent college of interior design following the . cases in the US. 2) Geographical preponderance of interior design major on Seoul and Gyeongsang Province is desired to be improved. 3) Curriculum of interior design, which is classified into introduction, history of interior design, theory of interior design, design of interior space, digital technology, design elements of inner space, technology of interior design, behavior of environmental psychology, design management and other subjects, was made into a table by divisions. To sum up, curriculum of interior design concentrates on basic design theory throughout all divisions. In connection with the selection of division by high school-graduates or their parents, design division is recommended for those who desire to major in design elements and design management, home economics division for those who desire to major in space design and behavior of environmental psychology and architectural division for those who desire to major in Interior space design, interior design technology and regulations. 4) This research can contribute to the settlement of desirable curriculum for the interior design education, thus upgrading the education level of the major in Korea.

Validation of High-Performance Liquid Chromatography Analysis on Phenolic Substances of Cirsium setidens and Sedative Effect of Pectolinarin as the Active Principle

  • Nugroho, Agung;Kim, Myung-Hoe;Lim, Sang-Cheol;Choi, Jong-Won;Choi, Jae-Sue;Park, Hee-Juhn
    • Natural Product Sciences
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    • v.17 no.4
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    • pp.342-349
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    • 2011
  • This study was performed to determine the composition of phenolic substances contained in the leaves of Cirsium setidens (Compositae), validate the high-performance liquid chromatography (HPLC) method, and determine the in vivo sedative effect of the main component pectolinarin. Six phenolic compounds isolated from C. setidens were spectroscopically identified as chlorogenic acid (1), hyperoside (2), 3,4-di-O-caffeoylquinic acid (3), caffeic acid methyl ester (4), linarin (5), and pectolinarin (6) and then used as standard compounds for HPLC analysis. HPLC proved to be precise, accurate, and sensitive for the simultaneous analysis of the phenolic substances. In particular, six compounds showed good regression ($R^2$ > 0.999) within test ranges and recovery was in the range of 95.4 - 104.8%. The content of pectolinarin was considerably higher (156.48 mg/g) than those of other phenolic substances including the other flavone glycoside, linarin (18.99 mg/g). The contents of other phenolic substances, in order, were chlorogenic acid (8.41 mg/g), 3,4-di-O-caffeoylquinic acid (5.74 mg/g), hyperoside (4.33 mg/g), and caffeic acid methyl ester (0.51 mg/g). Oral administration with compound 6 (10 and 20 mg/kg) enhanced the sleeping time induced by pentobarbital in mice, indicating that it has a sedative effect.

Quantitative Analysis of Ellagic Acid in Nymphaea tetragona Georgi Root by HPLC-UVD (HPLC-UVD를 이용한 수련(Nymphaea tetragona Georgi)의 Ellagic Acid 정량 분석)

  • Lee, Dong Young;Jeong, Eun Ju;Jeon, Byung Ju;Sung, Sang Hyun
    • Korean Journal of Pharmacognosy
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    • v.45 no.1
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    • pp.84-87
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    • 2014
  • A high performance liquid chromatography (HPLC) method for the quantitation of ellagic acid in Nymphaea tetragona was developed for the quality control of functional cosmetic ingredient, the extract of N. tetragona. Separation and quantitation were successfully achieved with a Kromasil C18 column ($5{\mu}m$, $250mm{\times}4.6mm$, i.d.) by isocratic elution of a mixture of acetonitrile containing 0.1% trifluoroacetic acid and water containing 0.03% phosphoric acid at a flow rate of 1.0 ml/min. The UV detector was used for the detection and the wavelength for quantitation was set at 254 nm. The presence of ellagic acid in the extract was determined by comparison of retention time and spiking with authentic standard. Analytical results showed good linearity ($R^2=0.99996$) in relatively wide concentration ranges. The R.S.D. for precision test was less than 3.0%. Recovery of the compound was 98.55~101.72% with R.S.D values less than 4.0%. In conclusion, this method has been successfully applied to the determination of ellagic acid in N. tetragona.

Simultaneous Determination of 11 Marker Compounds in Gumiganghwal-tang by HPLC-DAD and LC-MS

  • Weon, Jin Bae;Jung, Youn Sik;Ryu, Gahee;Yang, Woo Seung;Ma, Choong Je
    • Natural Product Sciences
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    • v.22 no.4
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    • pp.238-245
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    • 2016
  • Gumiganghwal-tang has been used for the treatment of common cold for a long-time. We developed an accurate and sensitive high performance liquid chromatography-diode array detection (HPLC-DAD) and electrospray ionization mass spectrometry method for the simultaneous determination of ferulic acid, baicalin, bergapten, methyl eugenol, glycyrrhizin, oxypeucedanin, wogonin, nodakenin, atractylenolide III, imperatorin, and atractylenolide I in Gumiganghwal-tang samples. The analytes were separated on a Shiseido C18 column ($5{\mu}m$, $4.6mm\;I.D.{\times}250mm$) with gradient elution with acetonitrile and 0.1% trifluoroacetic acid. Eleven compounds were quantitatively determined by HPLC-DAD and identified by LC-MS data. We also validated this method. The calibration curves of all the compounds showed good linear regression. The limits of detection and the limits of quantification ranged from 0.04 to 0.63 and from 0.12 to $1.92{\mu}g/mL$, respectively. The relative standard deviation values of intra- and inter-days of this method represented less than 2.9%. The recoveries were found to be in the range of 90.06 - 107.66%. The developed method has been successfully applied to the analysis of Gumiganghwaltang samples. The established HPLC method could be used to quality control of Gumiganghwal-tang.

Fatty Acid Composition of Adipose Tissues in Obese Mice and SD Rats Fed with Isaria sinclairii Powder

  • Ahn, Mi-Young;Seo, Yun-Jung;Ji, Sang-Deok;Han, Jea-Woong;Hwang, Jae-Sam;Yun, Eun-Young
    • Toxicological Research
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    • v.26 no.3
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    • pp.185-192
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    • 2010
  • Isaria sinclairii (Cicada Dongchunghacho) was studied as a potential crude natural food in powdered form. The role of tissue fatty acids in relation to the anti-obesity effects of I. sinclairii (IS) was examined by feeding the powder to SD rats ad libitum at 0, 1.25, 2.5, 5 and 10% (calculated about 8 g/kg) of the feed for a period of 3 months and 6 months. The fatty acid composition profile as indicated GC-MS, showed significantly slight dose-dependent increases in the levels of unsaturated fatty acids, particularly, arachidonic acid (C20: 4n6), oleic acid, linoleic acid, eicosadienoic acid, eicosapentaenoic acid (EPA) (C20: 5) concentration in the the ad libitum IS-fed groups compared to the control group in SD abdominal fat over 6 month period. Over viewing of the SD and Ob mice treated Isaria sinclairii powder; there were increases in the single (mono) unsaturated fatty acids ratio but decreases in polyunsaturated fatty acid. In IS-fed groups in proportion to the treatment period, this Dongchunghacho also induced an increase in the level of same result of unsaturated fatty acid in C57BL/6 obese (ob/ob) mice over a 6-month period treatment compared to those given 10% dry mulberry leaf powder (ML) or silkworm powder mixed with the standard diet.

New Measuring Method of Breast Volume Using Polyethylene Bag and Plaster Molding (투명 폴리백과 반창고를 이용한 간편한 유방 부피 측정법의 소개)

  • Lee, Seung Jong;Lee, Hye Kyung;Tark, Kwan Chul;Shin, Keuk Shun
    • Archives of Plastic Surgery
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    • v.33 no.5
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    • pp.676-679
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    • 2006
  • Purpose: Breast volume is one of the crucial measurements in preoperative planning and postoperative evaluation of the results in mammoplasty. There are several methods suggested by different authors, but there is still no commonly accepted standard methods for measuring breast volume. To help the surgeons to base their estimation on an objective evaluation, we developed a simple method using Polyethylene bag and plaster molding. Methods: After Polyethylene bags were put in suitable size on both breasts of the patient in upright position, silk plaster was molded on the surface evenly. Then molds can be obtained after marking boundaries of breasts with a pen. Breast volume measurement can be done by filling water in the molds and measuring it. Moreover, postoperative design for natural skin brassier was possible using the molds. Results: This method was applied to 2 patients for reduction mammoplasty and the breast volume measurement was simple, hygienic and accurate, done within 10 minutes. Conclusion: This method using Polyethylene bag and plaster molding has several advantages. 1. It is comparatively accurate regardless of the size and shape of patient's breasts in upright position. 2. Measurement time is short and inconvenience and shame of patients can be reduced by making molds after putting on Polyethylene bags. 3. It is relatively economical and uses easily available hygienic materials. 4. The postoperative shape and volume of breasts can be predicted by using molds preoperatively.