Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Validation of High-Performance Liquid Chromatography Analysis on Phenolic Substances of Cirsium setidens and Sedative Effect of Pectolinarin as the Active Principle

  • Nugroho, Agung (Department of Applied Plant Sciences, Graduate School, Sangji University) ;
  • Kim, Myung-Hoe (Department of Applied Plant Sciences, Graduate School, Sangji University) ;
  • Lim, Sang-Cheol (Department of Applied Plant Sciences, Graduate School, Sangji University) ;
  • Choi, Jong-Won (College of Pharmacy, Kyungsung University) ;
  • Choi, Jae-Sue (Department of Food Science and Nutrition, Pukyong National University) ;
  • Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University)
  • Received : 2011.11.29
  • Accepted : 2011.12.20
  • Published : 2011.12.30
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Abstract

This study was performed to determine the composition of phenolic substances contained in the leaves of Cirsium setidens (Compositae), validate the high-performance liquid chromatography (HPLC) method, and determine the in vivo sedative effect of the main component pectolinarin. Six phenolic compounds isolated from C. setidens were spectroscopically identified as chlorogenic acid (1), hyperoside (2), 3,4-di-O-caffeoylquinic acid (3), caffeic acid methyl ester (4), linarin (5), and pectolinarin (6) and then used as standard compounds for HPLC analysis. HPLC proved to be precise, accurate, and sensitive for the simultaneous analysis of the phenolic substances. In particular, six compounds showed good regression ($R^2$ > 0.999) within test ranges and recovery was in the range of 95.4 - 104.8%. The content of pectolinarin was considerably higher (156.48 mg/g) than those of other phenolic substances including the other flavone glycoside, linarin (18.99 mg/g). The contents of other phenolic substances, in order, were chlorogenic acid (8.41 mg/g), 3,4-di-O-caffeoylquinic acid (5.74 mg/g), hyperoside (4.33 mg/g), and caffeic acid methyl ester (0.51 mg/g). Oral administration with compound 6 (10 and 20 mg/kg) enhanced the sleeping time induced by pentobarbital in mice, indicating that it has a sedative effect.

Keywords

References

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