• Microbiology and Biotechnology Letters
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• v.46 no.3
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• pp.244-252
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• 2018

Protein disulfide isomerase A3 (PDIA3) is a major member of the protein disulfide isomerase (PDI) family. PDI proteins commonly reside in the endoplasmic reticulum and mediate important thiol-disulfide interchanges during post-translational protein folding. Unlike other PDI family members, PDIA3 is ubiquitous in various organ systems. However, its physiological activity varies in other tissues. PDIA3 has been associated with cancer, airway inflammation, neurodegenerative diseases, and metabolic diseases. However, the mechanisms of the association of PDIA3 with these pathological conditions remain unclear. Recombinant PDIA3 (rPDIA3) is needed to clarify the interactions between PDIA3 and certain physiological phenomena. In the present study, we aimed to produce highly purified rPDIA3 for use in pathological experiments. We expressed rPDIA3 with a histidine-enriched elongated peptide tag in Escherichia coli and obtained rPDIA3 at 97.8% purity using consecutive His-tag and reverse-phase chromatography. Elongated peptide tags screened from artificially designated library had dual functions for protein expression and simple purification.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.2
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• pp.668-675
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• 2018

The continuing urbanization and industrialization around the world has required a large amount of power. Therefore, construction of major infrastructure, including nuclear power plants in coastal areas, has accelerated. In addition, the intensity of natural disasters is increasing due to global warming and abnormal climate phenomena. Natural disasters are difficult to predict in terms of occurrence, location, and scale, resulting in human casualties and property damage. For these reasons, the disaster scale and damage estimation in coastal areas have become important issues. The present study examined the predictable weather data and regional ratings and developed estimating functions for wind wave damage based on the disaster statistics in the southern areas. The results of the present study are expected to help disaster management in advance of the wind wave damage. The NRMSE was used for verification. The accuracy of the NRMSE results ranged from 1.61% to 21.73%.

• Journal of Korea Multimedia Society
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• v.12 no.2
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• pp.323-328
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• 2009

Natural Phenomena are simulated to make computer users feel verisimilitude and be immersed in games or virtual reality. The important factor in simulating fluid such as water or sea using 3D rendering technology in games or virtual reality is real-time interaction and reality. There are many difficulties in simulating fluid models because it is controlled by many equations of each specific situation and many parameter values. In addition, it needs a lot of time in processing physically-based simulation. In this paper, I suggest simplified fluid-surface model in order to represent interaction between rigid body and fluid, and it can make faster simulation by improved processing. Also, I show movement of fluid surface which is come from collision of rigid body caused by reaction of fluid in representing interaction between rigid body and fluid surface. This natural fluid-surface model suggested in this paper is represented realistically in real-time using fluid dynamics veri similarly. And the fluid-surface model will be applicable in games or animation by realizing it for PC environment to interact with this.

• Journal of the Korean Institute of Landscape Architecture
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• v.35 no.3
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• pp.71-81
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• 2007

This study focused on the one and only temple of on the coastline of the West Sea, Kimje's Manghae Temple and Mt. Jinbong. The purpose of this study was to research the unique sense of place of the area by researching the location and view that clearly shapes the identity of the landscape. The following are the results. 1. The cultural landscape of Manghae Temple and the natural landscape of Mt. Jinbong have characteristics which take in the coastline and skyline, respectively. Specifically, from the existential vertical-horizontal images of "sky - land - Mt. Jinbong" and "moon - cloud - sea", an ascending reflection of the landscape surroundings can be found. 2. The "Sea - Manghae temple - Mt. Jinbong - sky" is the representation of the moderate 'inside-space' which belongs to the particular landscape area and which also shows the topophilia to Manghae Temple. Through this kind of interpretation, the sense of place of Manghae Temple and Mt. Jinbong reveals an intra-structure of an Imaging Landscape implying harmony and moderation, which is a unified organization of the surrounding phenomena(Temple and Mountain) and the essence (the doctrine of Buddhism) that matches Jin-muk's asceticism and lifestyle. 3. While the cultural landscape of Manghae Temple has a strong religious reference, the natural landscape Mt. Jinbong emphasizes the geography of the landscape. In other words, the motivating factor of Manghae Temple is a metaphorical sense of place such as through the "prospect of the sea" or "the Western Sea Paradise" and Mt. Jinbong, the landmark of the Kimje-Mankyung Plains and the focal point of the West Sea sunset, is highlighted as a simile for this sense of place. 4. Keeping this sense of place and territory respectively and showing the. bond with the sense of place which develops rhythmically and continuously, Manghae Temple and Mt. Jinbong are sublimated into a unified intra-structure, which reflects the Imaging landscape characteristics of "Mt. Jinbong, a focal point of the Mankyung Plains facing the West Sea" and "Manghae Temple, looking out to sea".

• Journal of the Korean earth science society
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• v.31 no.3
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• pp.246-258
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• 2010

A convective circulation box was redesigned after analyzing reasons why adolescent elementary school students could not derive a convective circulation concept from the convection circulation box experiments. Even though students were in the formal operational period of Piaget, the adolescents felt difficult to understand a concept of the natural phenomena they have never seen before. Thus, we designed a method to help students increase their scientific understandings about the concept through developing a miniature convective circulation box. Findings indicated that an application of redesigned convective circulation box in the classroom experiment significantly increased the students' understanding about the convective circulations of land and sea breezes, and as well as their participation in the activities. In addition, the redesigned convective circulation box motivated students to develop their scientific thinking skills by allowing them to decide where to put visible incenses inside the box and to directly observe the smoke currents circulation formed accordingly. Redesigning and using a convective circulationbox as a miniature of natural phenomenon helps students avoid having misconceptions. The biggest merits of the box are that it is observable in all directions, it provides much clearer convective circulations comparing to the extant box, and it requires low production costs.

• Journal of the Korean Society of Costume
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• v.61 no.3
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• pp.139-152
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• 2011

This research explores the idea of digilog for fashion; it would help to conclude the uncertain mixed-phenomena and words as a neologism and find a fundamental concept that would be a reference for a lot of uncertain new words that have been occurring even this year. Furthermore, this research is aimed at analyzing how digilog has been reflected on the contemporary fashion and directing the ways towards the future of fashion. The digilog fashion design is the expression of a combination of opposite extremes, that is to say the combination of a paradoxical mix and directly-opposed elements. The outward expression of fashion design with digilog can be classified into several ways. First of all, it tends to use the elements of paradoxical expression; for example, applying high-technology to express a primitive and natural material. The analog expresses unprocessed. Secondly, using high-tech machine to express the texture of handicraft or rough embroidery. Lastly, digital printing(DTP) is applied to enlarge the several or the whole parts of a natural object or sensitivity. As a result of the analysis, the inside view of the contemporary fashion with digilog delineate followings; it is the attitude that claims to go back to the past and accepts the combination of nature and high-technology to get calmness in the rapidly changing modern world. And it is the will to fly from the utterly exhausted reality through the recurrence of the past when there used to be a lot of energies and used to live in affluence.

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1100-1103
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• 2007

In proton therapy, the Bragg peak is spread out by modulating or degrading the energy of the particles to cover a well-defined target volume at a given depth. Proton transfer plays a key role in a variety of biological, the origin of the elements, tests of the standard model along with applications in medicine, industry and chemical phenomena such as water autoionization, fast proton diffusion, acid-base neutralization. We have studied the radiolysis of various natural resources and have evaluated the antioxidant activity of radiolysis products by proton beam. The most of antioxidant activities of natural resources were decreased with increasing proton fluence. Proton beam induced antioxidant activities both in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and 2,2'-azinobis(3-ethylbenzot hialozinesulfonic acid) cation radical $(ABTS^{.+})$ assay by a dose dependent fashion.

• Journal of Korean Academy of Nursing
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• v.22 no.3
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• pp.337-351
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• 1992

Nursing as it is practiced and taught, historically has been viewed as natural science. There are new movements to create a paradigm of nursing in the human sciences. A natural science methodologies elicit quantitative data from observable phenomena and reveal causal relationships. Human science methodology is the study of Unitary Man's participative experience in a situation, the simultaneity paradigm. In a theory of nursing rooted in human science assumptions about man and health are synthesized, and the practice of nursing is continuously expanded through research. To find independent nursing interventions especially cognitive nursing intervention models it has been necessary to consult a multitude of journals and text sources for references, and no one nursing textbook can be used in help patients achieve nursing goals. The goals of nursing in the simultaneity paradigm focus on the quality of life from the person's perspective. Cognitive interventions based on the person's perspective were selected from those that colleagues deemed appropriate to nursing and those that were identified in the nursing literature. They were supportive nursing care, reminiscence, meditation, relaxation and imagery. Nurses have been reluctant to make diagnoses, implement actions, and assume responsibility for this actions and this is of concern but can be understood because nurses have had little exposure to action and lack an intervention armamentarium from with which to choose actions to achieve nursing goals. Efforts in nursing education and nursing service are required to remedy this problem. Nurses must be convinced of the challenge and excitement associated with autonomous functioning. It is a characteristic of the true professional. Traditionally, nursing has prescribed one method for handling a situation. Fundamental nursing texts usually only present one way to handle a situation, because alternative interventions to achieve a client goal may be available. Considerably more research is necessary before these can be prescribed. However, unless a first step is taken, progress will not be made. The quality of health care or nursing care is enhanced when nurses transform dilemas into commited action. This is apparent from widespread experiences of nurses.

• Journal of Korean Academy of Nursing
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• v.29 no.6
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• pp.1263-1272
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• 1999

This study was alone to identify the meaning and the essence of the menopause experience by means of the Colaizzi’s phenomenological method. The participants were eight women, 48-60 years old, who were experiencing perimenopause or postmenopause. They were selected using a theoretical sampling technique. Data were collected through in-depth interviews from April 6 to May 20, 1999. The interviews took from 40 to 90 minutes. Interviews were tape- recorded and analyzed using the constant comparative method. Significant statements from data were extracted. From these formulated meanings, 18 themes, 8 clusters of themes and 3 catagories were constructed. Final descriptions were found to be valid through the interviewee validation process. Essential themes of the menopausal experience which emerged were “coming of change”, “inclined to deny the in mind”, and “adapted life”. Menopause, as coming of change was the turning point in the women’s life cycle acompaning changes, in body and mind and marital life changes. Especially, the unpredictable menopausal changes were an opportunity to hide the fact of menopause and not to talk with anybody. Such a phenomenon reflected on the psychological attributes of menopausal woman such as an inclination to deny in the mind. Their experiences of menopause as adapted life were generally regarded as signalling the end of fertility and were interpreted as a natural developmental process that indicates a life transition. In conclusion, participants depicted the menopause as a natural stage in the life cycle. In order to help women have a positive life change and self accomplishment, nurses need to understand the essential themes of the menopausal experience. Thus, the importance of an open approach in eliciting the phenomena of menopause cannot be overemphasized. In conclusion, the results of this study suggest that exercise program is one of the effective interventions to improve the self-efficacy and also to lower the fatigue in the sample of female college students.

• Journal of the Korean Magnetics Society
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• v.26 no.6
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• pp.196-200
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• 2016

In this study the mechanisms of magnetization reversal and magnetic interaction effects on activation volumes for Sr-ferrite with different particle sizes are investigated. The activation volumes of C2 sample are larger than those of C3 sample in the range of low magnetic fields. But the fields above the coercivity of sample C2, the activation volumes of both samples are decreased linearly with increasing the applied magnetic field. These phenomena can be explained by the strengths of two critical fields representing the reverse domain nucleation field and the domain wall pinning field as well as the strength of dipolar interaction.