• Journal of environmental and Sanitary engineering
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• v.5 no.2
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• pp.63-80
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• 1990

A sensitive and simple analytical system for the simultaneous determination of residual oxytetracycline, tetracycline, and doxycycline in honey was described, and that the analytical method for determination of residual propionic acid in honey was established. Experimental subjects were purchased four kinds of honey, native kind honey, acaccia honey, mixed floral honey, chestnut honey in Kyung Sang Nam Do. Several microbiological methods are available to determine tetracycline antibiotecs(TCs) in foods but their precision apears to be variable and the specificity is questionable. These methods are considered to be not suitable for analysis of tetracycline antibiotics in honey because honey itself has bacteriostatic action. For determination of tetracycline antibiotics in honey, therefore the High Performance Liquid Chromatography(HPLC) method was applied, and the propionic acid were determined by Gas Chromatography(5.C). Ethylacetate, as an extract solvent, was found to be suitable for seperation of TCs in honey, but methanol and acetone were not. The recoverly rate of Oxytetracycline(OTC), Tetracycline(TC), Doxycycline(DC) from honey spiked at a level of 10 $\mu $g/g were 97%, 89%, and 91%, respectively. The cailbration curve in TCs was linear expression from 2$\mu $g/ml to 10$\mu $g/ml. As the results of analysis, the residual tetracycline antibiotics were not detected in the 100 samples of honey. The recovery rate of propionic acid from honey spiked at level of 10$\mu $g/g was 98.3% , and the calibra lion curves were linear expression from 21$\mu $g/ml to 101$\mu $g/ml. As the results of analysis, the residual propionic acid was not detected in the 100 samples of honey. Retention time(min) of OTC, DC, and TC were 3.35, 4.61, and 5.30 minutes at the conditions of table 2, respectively, and retention time(min) of propionic acid was 3.50 minutes at the conditions of table 3. The residual TCs and propionic acid were not detected in the 100 samples of honey, but there is a possibility that antibiotics or propionic acid will be to remain in honey if they are used during product period in order to prevent putrefaction of honey-bee.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.4
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• pp.680-685
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• 1994

Total nitrogen content, proline, free amino acids, sugars, invertase and diastase activities in native bee-honey (NBH) and foregin bee-honey (FBH) harvested from our different areas of Kangwon , Korea were determined. The total nitrogen contents of NBH and FBH were 0.077$\pm$0.033mg% and 0.055$\pm$0.022mg%, respectively. Proline content in NBH was 42$\pm$10mg% and waqs found to be much lower than that in FHB. Phenylalanine and proline were major free amino acids both in NBH and FBH . The content of total free amino acid in FBH were twice as much as that in NBH , however isomaltose content in NBH were almost two times more than that in FBH. Other sugars including fructose, glucos , sucrose and maltose were also analyzed. No differences were found between NBH and FBH in invertase activities, but comparing to these of FBH ,lower values of diastase activity in NBH were observed.

• Korean Journal of Food Science and Technology
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• v.29 no.6
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• pp.1082-1088
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• 1997

To compare the characteristics between native bee honey (NBH) and foreign bee honey (FBH), NBH harvested from eight different areas of Kangwon, Korea and FBH harvested from Chuncheon, Kangwon, Korea and from other nations were investigated by measuring the contents of moisture, sugar, 5-hydroxymethyl furfural (HMF), and pollen. Our results showed that contents of moisture in NBH and FBH were $16.4{\sim}23.0%$ and $15.8{\sim}21.0%$, respectively. Sugar contained in bee honey such as fructose, glucose, sucrose, maltose, and isomaltose were also analyzed. No difference were found between NBH and FBH in pH. The contents of HMF in NBH and FBH were $0.0{\sim}19.2\;mg/kg$ and $0.0{\sim}85.8\;mg/kg$, and was found that NBH is much lower than that in FBH. And production of HMF were accelerated by conditions of storage. Distributions of pollen in NBH were $0.4{\sim}88.3{\times}10^4/g$, which were significantly different from $0.0{\sim}0.4{\times}10^4/g$ obtained from FBH. According to those results, the methods used in this study are not suitable for identification of difference between NBH and FBH.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.1-5
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• 1998

To find out the difference between native bee-honey (NBH) and foreign bee-honey (FBH), quantification of honey protein and investigation of specific protein in NBH were carried out by SDS-PAGE. Contents of honey protein in NBH and FBH were measured by Bradford and Lowry method. The contents of protein determined by Bradford method were $0.1{\sim}3.3\;mg/g$ in NBH and $0.2{\sim}1.6\;mg/g$ in FBH, and by Lowry method were $12.9{\sim}45.7\;mg/g$ in NBH and $15.8{\sim}27.1\;mg/g$ in FBH. In order to investigate the distribution of bee honey proteins, the SDS-PAGE was performed. The results showed that molecular weight of the major proteins in NBH and in FBH were 56 kDa and 59 kDa. respectively. Therefore, it was confirmed that the difference between NBH and FBH can be identified visually by SDS-PAGE analysis. The major proteins in NBH and FBH were purified through two step chromatography, and the obtained proteins were used as marker protein in SDS-PAGE to discriminate NBH and FBH.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.4
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• pp.675-679
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• 1994

Minerals, hydroxymethyfufural (HMF) and vitamins in native bee-honey (NBH) harvested from four different areas of Kangwon, korea were analyzed . Ash content of NBH varied from 0.26 to 0.50 % with the mean and standard deviation values of 0.32$\pm$ 0.17% and those of FBH varied from 0.15 to 0.58%(0.32$\pm$0.37) . Among analyzed minerals, the concentration of K,Ca, Mg, Fe and P except Na in NBH were found to be higher than those in FBH. The amounts of K and NA in NBH were 1200-3200ppm(2000 $\pm$770ppm) and 35-50ppm(38$\pm$6ppm), and those in FBH were 1100-3300ppm(1900$\pm$ 1700ppm) and 32-72ppm(49$\pm$17ppm), respectively. The mean value of Na to K ratio expressed as Na/Kx10$^3$ was 20$\pm$4 for the NBH and 41$\pm$25 for the FBH. HMF levels in NBH were 0.50$\pm$0.22mg% which was twice as much as those in FBH. Ascorbic acid and riboflavin in NBH were present at the range of 2.2-4.0mg% and 0.17-0.24mg% , respectively, of which were not significantly different from those obtained from the FBH.

• Journal of Apiculture
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• v.33 no.4
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• pp.269-275
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• 2018

Beekeeping status of Apis cerana with emphasis of experiences overcoming sacbrood virus disease are presented. Social bee fauna are rich in Vietnam with 6 honeybee species (Apis laboriosa, Apis dorsata, Apis mellifera, Apis cerana, Apis andrenifomis, Apis florea); 8 stingless bee species (Trigona laeviceps, Trigona ventralis, Trigona pagdeni, Trigona gressitti, Trigona fuscobalteata, Trigona capenteri, Trigona scintillans Trigona iridipenis) and 2 bumble bee species (Bumbus haemorrhoidalis, B. breviceps). All of them are native except A. mellifera which was introduced in1887. These bees are slated for conservation by the Ministry of Agriculture & Rural Development. Honey and other bee products are mainly harvested from 3 species including A. cerana, A. mellifera and A. dorsata. The manageable species (A. cerana and A. mellifera) are increasing in number, reaching about 1,500,000 beehives. Vietnam is the second largest honey exporter in Asia, with a total of about 48,000 tons of honey exported to the international market in 2014. A. cerana plays an important role in poverty alleviation in mountainous and remote areas of Vietnam. Honeybee suffers from various diseases of Sacbrood virus disease (SBV), European foulbrood (EFB), Nosema, and parasitic mites of Tropilaelaps mercedes and Varroa destructor. Most of these diseases can be resolved with biocontrol methods. For the parasitic mites, Vietnamese beekeepers usually apply formic acid.

• Korean Journal of Food Science and Technology
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• v.42 no.6
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• pp.643-647
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• 2010

This research was carried out to investigate residues of neomycin, streptomycin, dihydrostreptomycin, amitraz, 2,4-dimethylaniline (one of amitraz's metabolites), and coumaphos in honey in order to intensively control their use following the establishment of Korean maximum residue limits (MRLs) for veterinary drugs in honey in 2007. To monitor for residues, 110 honeys and food products with honey were collected and analyzed. The collected honeys included acasia, mixed flower, chestnut, rape flower, jujube, and native types. Neomycin, streptomycin, dihydrostreptomycin, oxytetracycline, and amitraz were not detected among samples. Coumaphos was found in the Korean acasia honey at 0.02 mg/kg, but its concentration was under the MRL (0.1 mg/kg) for coumaphos. According to the results, there were no violations of the Korean MRLs of veterinary drugs in honey.

• Horticultural Science & Technology
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• v.16 no.3
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• pp.361-363
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• 1998

Cymbidium goeringii native to Korea and other orchid plants were pot-grown from spring to autumn under the greenhouse conditions, and were subjected to artificial pollination to elucidate the compatibility by revealing viable seed formation. A notable compatibility was found when Cym. goeringii was selfed and was crossed with either Cym. ensifolium, Cym. kanran, Cym. sinense, Cym. sinense for. albo-jucundissimum, Cym. 'Crystal Cherry Angel', or Cym. 'Anmitsu Hime'. Cym. goeringii, however, did not show such compatibility when crossed with either Cym. faberi, Cym. aloifolium, Dendrobium chrysotoxum, or Phalaenopsis spp. RAPD analysis indicated that taxa relationship between Cym. goeringii and either Cym. faberi or Cym. aloifolium (respective chromosome number, 2n=40) was distant, showing no compatibility, and even more distant in the case of cross-pollination between Cym. goeringii and either Dendrobium chrysotoxum or Phalaenopsis spp. having different chromosome number from all Cymbidium species.

• BMB Reports
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• v.36 no.6
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• pp.572-579
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• 2003

An expressed sequence tag (EST) library was established from the hypopharyngeal glands of Apis cerana. Sixty-six recombinant clones, possessing inserts >500 bp, were randomly selected and unidirectional sequenced. Forty-two of these (63.6%) were identified as homologues of Major Royal Jelly Proteins families 1, 2, 3, and 4 of A. mellifera (AmMRJP) for which MRJP1 was the most abundant family. The open-reading frame of the MRJP1 homologue (AcMRJP1) was 1299 nucleotides that encoded 433 deduced amino acids with three predicted N-linked glycosylation sites. The AcMRJP1 sequence showed 93% and 90% homologies with nucleotide and deduced amino acid sequences of AmMRJP1, respectively. Two complete transcripts of apisimin, and one and two partial transcripts of $\alpha$-glucosidase and glucose oxidase, were also isolated. In addition, the royal jelly proteins of A. cerana were purified and characterized using Q-Sepharose and Sephadex G-200 column chromatography. The native forms of protein peaks A1, A2, B1, and C1 were 115, 55, 50, and 300 kDa, respectively. SDS-PAGE analysis indicated that A1 and C1 were dimeric and oligomeric forms of the 80 kDa and 50 kDa subunits, respectively. The ratio of the total protein quantities of A1 : A2 : B1 : C1 were 2.52 : 4.72 : 1 : 12.21. Further characterization of each protein, using N-terminal and internal peptide sequencing, revealed that the respective proteins were homologues of MRJP3, MRJP2, MRJP1, and MRJP1 of A. mellifera.

• Journal of Apiculture
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• v.33 no.4
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• pp.227-237
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• 2018

The majority parasitic bee mites of Thailand in genus Tropilaelaps are infesting colonies of native bees (Apis dorsata) and introduced bees (A. mellifera). The investigation aims to study morphological and genetic variation of Tropilaelaps mites infected different hosts. Adult mites were collected from honey bee brood throughout Thailand. Traditional and geometrical morphometrics were measured on photograph by using TPS program. Additional, COI gene variations were examined by PCR-RFLP and nucleotides sequencing. Tree of mites relationships were constructed by NJ and MP assumptions. Morphometric results indicated T. mercedesae were major species infesting on A. dorsata and A. mellifera. Mophological variation represented at anal and epigynial plate, which the shape of the anal plate apex margin has been key character to identify between T. mercedesae (bell to blunt shape) and T. koenigerum (pear shape). However, the discriminant analysis suggested that geometric results were potential to classify Thai Tropilaelaps populations from different hosts better than traditional morphometric. Otherwise, PCR-RFLP clearly detected the site of Dra I and Xba I digestion of Thai Tropilaelaps morphotypes. The COI sequences of T. koenigerum were founded infesting only A. dorsata in Thailand and four sequences that related to the Thai T. mercedesae morphotypes. The NJ and MP tree were clearly classified Thai Tropilaelaps species which were suggested both from morphological and molecular analysis. This information might be basically of taxonomic status, but this should have implication for controlling these mites in Thailand and other countries.