• Journal of Electrodiagnosis and Neuromuscular Diseases
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• v.20 no.2
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• pp.77-83
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• 2018

Small fiber neuropathy (SFN) mainly affects thinly myelinated $A{\delta}$-fibers and unmyelinated C-fibers presented with neuropathic pain like burning feet or numbness. Many conditions are known as a causes of SFN, metabolic derangement, especially glucose intolerance, is the most frequent cause of SFN. It has been hard to diagnose SFN because there has been lack of specialized test for small nerve fiber. Quantification of intraepidermal nerve fiber density using skin biopsy is promising method to diagnose SFN. A skin biopsy also could give helps to research pathophysiology of SFN by specialized stain method.

• Journal of Life Science
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• v.21 no.6
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• pp.851-857
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• 2011

Sulforaphane (SFN) is an isothiocyanate, isolated from glucoraphanin in broccoli and other cruciferaous vegetables. Recent studies have revealed that SFN induces anti-proliferation and apoptosis by cell cycle arrest in various cancer cells. In this study, we investigated the effect of SFN induced apoptosis in chondrosarcoma HTB-94 cells. SFN caused suppression of proliferation and apoptosis in a dose-dependent manner as determined by cell phenotype, MTT assay and FACS analysis in HTB-94 cells. Treatment of SFN led to caspase-3 activation and p53 accumulation as determined by Western blot analysis. Also, SFN significantly induced DNA fragmentation and nuclear degradation though activation of caspase-3, as detected by DNA electrophoresis and immunostaining, respectively. Our results indicate that SFN-induced apoptosis was regulated by p53 and caspase-3 dependent pathways. Furthermore, SFN may act as a potent anti-proliferation agent, and as a promising candidate for molecular-targeting chemotherapy against human chondrosarcoma cells.

• Journal of Veterinary Science
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• v.21 no.6
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• pp.91.1-91.15
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• 2020

Background: Sulforaphane (SFN) is an isothiocyanate compound present in cruciferous vegetables. Although the anti-inflammatory effects of SFN have been reported, the precise mechanism related to the inflammatory genes is poorly understood. Objectives: This study examined the relationship between the anti-inflammatory effects of SFN and the differential gene expression pattern in SFN treated ob/ob mice. Methods: Nitric oxide (NO) level was measured using a Griess assay. The inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression levels were analyzed by Western blot analysis. Pro-inflammatory cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, and IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). RNA sequencing analysis was performed to evaluate the differential gene expression in the liver of ob/ob mice. Results: The SFN treatment significantly attenuated the iNOS and COX-2 expression levels and inhibited NO, TNF-α, IL-1β, and IL-6 production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. RNA sequencing analysis showed that the expression levels of 28 genes related to inflammation were up-regulated (> 2-fold), and six genes were down-regulated (< 0.6-fold) in the control ob/ob mice compared to normal mice. In contrast, the gene expression levels were restored to the normal level by SFN. The protein-protein interaction (PPI) network showed that chemokine ligand (Cxcl14, Ccl1, Ccl3, Ccl4, Ccl17) and chemokine receptor (Ccr3, Cxcr1, Ccr10) were located in close proximity and formed a "functional cluster" in the middle of the network. Conclusions: The overall results suggest that SFN has a potent anti-inflammatory effect by normalizing the expression levels of the genes related to inflammation that were perturbed in ob/ob mice.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.4
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• pp.21-27
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• 2014

The broadcasting of the analog TV was terminated in 2012 and was converted into the broadcasting of the digital TV, so methods for arrival of DAB(Digital Audio Broadcasting) are planned recently. Thus, with digitization of analog broadcasting and appearance of new service, it takes effort to improve efficiency of broadcasting frequency. T-DMB which came in 2005 uses existing analog TV band and its channel is allocated inefficiently in order to avoid interference between mutual services. However, the foundation which can afresh reorganize T-DMB channel is building up because the broadcasting of the analog TV was terminated. So, this paper proposes the method for national SFN composition of T-DMB considering existing local broadcasting in order to provide better service to users as well as improve efficiency of broadcasting frequency.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.3
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• pp.241-247
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• 2015

Ultraviolet (UV) radiation-induced loss of dermal extracellular matrix is associated with skin photoaging. Recent studies demonstrated that keratinocyte-releasable stratifin (SFN) plays a critical role in skin collagen metabolism by inducing matrix metalloproteinase 1 (MMP1) expression in target fibroblasts. In the present study, we examined whether SFN released from UVB-irradiated epidermal keratinocytes increases MMP1 release from dermal fibroblasts, and whether these events are affected by p-coumaric acid (p-CA), a natural phenolic compound with UVB-shielding and antioxidant properties. HaCaT cells were exposed to UVB in the absence and presence of p-CA, and the conditioned medium was used to stimulate fibroblasts in medium transfer experiments. The cells and media were analyzed to determine the expressions/releases of SFN and MMP1. UVB exposure increased SFN release from keratinocytes into the medium. The conditioned medium of UVB-irradiated keratinocytes increased MMP1 release from fibroblasts. The depletion of SFN using a siRNA rendered the conditioned medium of UVB-irradiated keratinocytes ineffective at stimulating fibroblasts to release MMP1. p-CA mitigated UVB-induced SFN expression in keratinocytes, and attenuated the MMP1 release by fibroblasts in medium transfer experiments. In conclusion, the present study demonstrated that the use of UV absorbers such as p-CA would reduce UV-induced SFN-centered signaling events involved in skin photoaging.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.1
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• pp.95-103
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• 2015

The Korea terrestrial broadcasting has TV and Radio, DMB. The TV broadcasting was converted from the analogue method to the digital method at the end of 2012. However the radio broadcasting is still in analogue method. The introduction of digital radio technology will bring many benefits such as high-quality of sound, various additional services and increase of efficiency in frequency utilization. In many countries, the radio broadcasting is been servicing in digital method. The discussion was begun on this year for the introduction of digital radio technology in Korea. So, to prepare against introduction of DAB system among digital radio technology, this paper proposes channel allocation method for each local radio broadcasting and the composition of national SFN, and proves the proposed method through the interference analysis.

• Journal of Broadcast Engineering
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• v.18 no.4
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• pp.572-588
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• 2013

In this paper, 4K-UHDTV or 8K-UHDTV and UHD-3DTV that the next generation broadcasting implementation and the possibility of direct receiving environment construction is analyzed on the terrestrial broadcasting. Particularly, we investigated the possibility by analyzing the previous and related works with regard to UHDTV transmission by DVB-T2 that is one of the best commercialized transmission mode. In order that the UHDTV broadcasting succeeds once again after completion of digital terrestrial switch over at the end of 2012, the ultra high resolution image transfer is important. However, the direct, the indoor and ubiquitous receiving environment is important in not only TV but also the personal type multimedia terminal in the sense of UHDTV service penetration. Therefore, in this paper, by using SFN and high error-correcting mode in DVB-T2 standard, the efficient frequency utilization and effective reception environment construction is illustrated. Particularly, SFN network constitution by 2 mutually different frequencies including the VHF bandwidth and UHF band, and etc. is shown. And the method that builds the free wireless receive environment by using SFN low power radio repeater and for home use gap filler is proposed. And the effect and frequency amount required are presented, when UHDTV broadcasting use 10MHz bandwidth.

• Journal of Broadcast Engineering
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• v.14 no.3
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• pp.311-321
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• 2009

In a single frequency network (SFN) for Advanced Television Systems Committee (ATSC) terrestrial digital television (DTV) system, the interference due to the use of same frequency among multiple transmitters or repeaters is inevitable for receivers. This problem can be solved by adjusting transmit power and time of each transmitter and repeater. To adjust SFNs, the ATSC recommended practice (RP) introduces a transmitter identification (TxID) signal which is embedded in a signal from each transmitter or repeater. This paper proposes an efficient method to estimate the individual reception power from each transmitter or repeater based on the channel profile of SFN and the total reception power. Moreover, field test results are provided to evaluate the performance of the proposed method.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2011.07a
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• pp.130-132
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• 2011

본 논문은 지상파 방송망 내 전계강도가 낮은 지역에 최소한의 데이터 전송률을 보장하기 위한 계층변조와 다중경로 채널 극복 및 수신 성능 향상을 위한 다중 안테나 수신기법을 SFN에 적용하여 제안하였다. 계층변조는 기존의 데이터 스트림에 부가적인 데이터 스트림을 삽입함에 따라 지상파 3D HDTV 방송 서비스는 더 많은 유효 전송률을 확보하고 고품질 비디오 서비스를 제공할 수 있었다. 그러나 기본 서비스의 품질을 유지하면서 부가적인 서비스의 품질을 보장하기 위한 대책이 필요하다. 그래서 송, 수신 안테나의 이득을 올리기 위해 다중 안테나 사용 방식을 채용하고, 그 수신 성능을 다중 안테나 위치에 따라 비교해 보았다. 그 결과, 수신 안테나가 한 쪽의 송신 안테나에 가까워질수록 실질적인 SNR이 증가하여 BER 성능이 향상되는 것을 확인하였다.

• Journal of Broadcast Engineering
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• v.16 no.1
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• pp.64-72
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• 2011

In a Single Frequency Network (SFN) for Advanced Television Systems Committee (ATSC) terrestrial digital television (DTV) system, the interferences induced by the multiple transmitters and/or repeaters using same frequency are unavoidable. To ease the interference handing in the SFN for ATSC DTV system, transmitter identification (TxID) signal is recommended in the ATSC terrestrial DTV system. The TxID signal is embedded in the ATSC DTV signal and transmitted from each transmitter and repeater within the SFN and in ATSC recommended practice (RP) A/111, Kasami sequence is recommended as the TxID signal. In this paper, we propose an additional data transmission scheme using the TxID signal. In the proposed scheme, the Kasami sequence not only acts as the conventional TxID of the SFN for ATSC terrestrial DTV system but also carriers the additional data bits on its polarity and the code phase.