• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.8
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• pp.417-428
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• 2020

Housekeeping genes are expressed in cells of all organisms and perform basic cellular functions such as energy generation, substance synthesis, cell death, and cell defense. Accordingly, the expression levels of housekeeping genes are relatively constant, and thus they are used as reference genes in gene expression studies, such as protein expression and mRNA expression analysis of target genes. However, the levels of expression of these genes may be different among various tissues or cells and may change under certain circumstances. Therefore, it is important to select the best reference gene for specific gene expression research by exploring the stability of housekeeping gene expression. This review summarizes housekeeping genes found in humans, chickens, pigs, and rats in the literature and estimates expression stability using geNorm, NormFinder, and BestKeeper software. The most suitable reference housekeeping gene can selected based on expression stability according to the experimental conditions of the gene expression study and can thus be applied to data normalization.

• Molecular & Cellular Toxicology
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• v.5 no.1
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• pp.32-43
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• 2009

Acetaminophen (APAP) overdose is known to cause severe hepatotoxicity mainly through the depletion of glutathione. In this study, we compared the cytotoxic effects of APAP on both a normal murine hepatic cell line, BNL CL.2, and its SV40-transformed cell line, BNL SV A.8. Gene expression profiles for APAP-treated cells were also obtained using microarray and analyzed to identify differences in genes or profiles that may explain the differences of susceptibility to APAP in these cell lines. These two cell lines exhibited different susceptibilities to APAP (0-$5,000{\mu}M$); BNL SV A.8 cells were more susceptible to APAP treatment compared to BNL CL.2 cells. A dose of $625{\mu}M$ APAP, which produced significant differences in cytotoxicity in these cell lines, was tested. Microarray analysis was performed to identify significant differentially expressed genes (DEGs) irrespective of APAP treatment. Genes up-regulated in BNL SV A.8 cells were associated with immune response, defense response, and apoptosis, while down-regulated genes were associated with catalytic activity, cell adhesion and the cytochrome P450 family. Consistent with the cytotoxicity data, no significant DEGs were found in BNL CL.2 cells after treatment with $625{\mu}M$ APAP, while cell cycle arrest and apoptosis-related genes were up-regulated in BNL SV A.8 cells. Based on the significant fold-changes in their expression, a genes were selected and their expressions were confirmed by quantitative real-time RT-PCR; there was a high correlation between them. These results suggest that gene expression profiles may provide a useful method for evaluating drug sensitivity of cell lines and eliciting the underlying molecular mechanism. We further compared the genes identified from our current in vitro studies to the genes previously identified in our lab as regulated by APAP in both C57BL/6 and ICR mice in vivo. We found that a few genes are regulated in a similar pattern both in vivo and in vitro. These genes might be useful to develop as in vitro biomarkers for predicting in vivo hepatotoxicity. Based on our results, we suggest that gene expression profiles may provide useful information for elucidating the underlying molecular mechanisms of drug susceptibility and for evaluating drug sensitivity in vitro for extrapolation to in vivo.

• The Journal of Industrial Distribution & Business
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• v.9 no.7
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• pp.61-70
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• 2018

Purpose - The purpose of this research is reflected on the rapid development of online tourism industries. The study was to establish the strategy for Korean tourism enterprises to develop tourist commodities suitable for Chinese tourists and attract them to visit Korea by the empirical analysis of the relation between repurchase intention of tourists and its premise variables (e-service quality, perceived value and satisfaction). Research design, data, and methodology - This research carried out a questionnaire survey on Chinese tourists who visited Korea with experience of using the online travel agency web sites. A total 398 answers were recovered, 41 of them were excluded due to the dishonest answers and 357 of them were finally analyzed. The data was analyzed with IBM SPSS AMOS 22.0. Results - The research results show that in the online travel agency web site e-service quality, convenience, interactivity, information validity, credibility had a positive impacts on perceived value and satisfaction. The perceived value of online travel agency website users has positive impart on satisfaction and repurchase intention. Satisfaction of online travel agency web site users have positive impacts on repurchase intention. But safety has no impact on perceived value while positive impacts on satisfaction was affected. Conclusions - First, in the online travel agency web site e-service quality, safety has no impact on perceived value while it was shown to have positive impacts on satisfaction because the users of online travel agency web sites believe that the protection of personal information, the defense of cracker and the safeguard of payment security are the basic premises of website operation. Although safety does not have impacts on perceived value, users benefits will suffer damage when hacker intrusion and other accidents occur so that online travel agency web sites should not ignore the security concerns. Second, credibility is a major concern for online travel agency web site users. At this time, it is necessary for the web site to establish a system to display both the commodity information and the using experience published on the user's SNS, thus improving the credibility of the website information.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.10a
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• pp.864-867
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• 2003

GPS (Global Positioning System) made by the Department of Defense in U.S.A is positioning system to use satellite and initially it has been used only for the military forces but open to civilian in about 1987. This system has widely been used for not only surveying land, but also car navigation on the street and means to build up the data of the GIS. With GPS, recently our country is accelerating to make imbeded system and also the study on imbeded system is well under way. For example, Car navigation and the construction of the Seokang bridge between Willson Arch at Han river by using DGPS were evaluated as successful model to lead accurate location with the precision of the cm. The examples of the project performance with GPS has gradually been extended to the each department organization of the local and central government. for the example, It is true that BIS(Bus Information System) is widely spreading out. In addition, the study on the Distribution Maintenance System is expected to be well in progress to take advantage of GPS based on the data base of the NGIS(National Geography Institute System) of the NGI(National Geography Institute). This paper shows that we embodied not only the large imbeded system for car and finding the location in Korean Land Corporation but also the protype of the kinematics Wrist Held which is easily portable to pedestrian, climber and marathon runner.

• Korean Journal of Remote Sensing
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• v.24 no.4
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• pp.309-324
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• 2008

In this study the retrieval algorithms have been developed to retrieve total precipitable water (TPW) from Terra/Aqua Moderate Resolution Imaging Spectroradiometer (MODIS) infrared measurements using a physical iterative retrieval method and a split-window technique over East Asia. Retrieved results from these algorithms were validated against Defense Meteorological Satellite Program (DMSP) Special Sensor Microwave/Imager (SSM/I) over ocean and radiosonde observation over land and were analyzed for investigating the key factors affecting the accuracy of results and physical processes of retrieval methods. Atmospheric profiles from Regional Data Assimilation and Prediction System (RDAPS), which produces analysis and prediction field of atmospheric variables over East Asia, were used as first-guess profiles for the physical retrieval algorithm. We used RTTOV-7 radiative transfer model to calculate the upwelling radiance at the top of the atmosphere. For the split-window technique, regression coefficients were obtained by relating the calculated brightness temperature to the paired radiosonde-estimated TPW. Physically retrieved TPWs were validated against SSM/I and radiosonde observations for 14 cases in August and December 2004 and results showed that the physical method improves the accuracy of TPW with smaller bias in comparison to TPWs of RDAPS data, MODIS products, and TPWs from split-window technique. Although physical iterative retrieval can reduce the bias of first-guess profiles and bring in more accurate TPWs, the retrieved results show the dependency upon initial guess fields. It is thought that the dependency is due to the fact that the water vapor absorption channels used in this study may not reflect moisture features in particular near surface.

• Korean Journal of Remote Sensing
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• v.35 no.5_2
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• pp.831-840
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• 2019

Recently, the span length of long-span bridges is getting longer. As a result, it has been suggested that a new concept called 'super long-span bridge'. In case of super long span bridges, the structure is being complicated and the importance of structural stability is being emphasized. However, until recently, the most commonly used sensors (dual axis clinometer, anemometer, strain gauge, etc.) have got limit about the bridge monitoring. Consequently, we researched the application of a Global Navigation Satellite System (GNSS) to improve the limit of the existing sensors. In this study, the dual axis clinometer, the anemometer and the strain gauge together with the GNSS were used to analyze the behavior of a super-long suspension bridge. Also, we propose the detailed method of bridge monitoring using the GNSS. This study consisted of three steps. First step calculated the absolute coordinates of the towers and the longitudinal axis direction of the study bridge using the GNSS. In second step, through the analysis of the long-term behavior in shortly after construction, we calculated the permanent displacement and evaluated the stability of main towers. Third step analyzed the behavior of bridge by the wind direction and was numerically indicated. Consequently, the bridge measurement using the GNSS appeared that the acquired data is able to easy processing according to the analysis purpose. If we will use together the existing measurement sensors with the GNSS on the maintenance of the super long-span bridge, we figure each error of measurement data and improve the monitoring system through calibration. As a result, we acquire the accurate displacement of bridge and figure the behavior of bridge. Consequently, we identified that it is able to construct the effective monitoring system.

• Journal of Digital Convergence
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• v.13 no.11
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• pp.141-148
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• 2015

In this paper, for developing and implementing the HLS(Http Live Streaming) transmitter based embedded linux which is operated easily and cheap and lower power, portable in all networks and client environments compared to exist video live streaming transmitters. We design the developed HLS transmitter hardware using the Arm11 core and then porting the Embedded Linux OS(Operating System) and implementing the HLS protocol using the open source FFmpeg and Segmenter. For proving the performance of developed HLS transmitter, we make the testing environment for testing the performance of HLS transmitter including the notebook, iPhone, android Phone, Notebook and then analysis the received video in the client displayer. In this paper, we suggest the developed HLS transmitter performance data values higher than the Apple company's HLS recommended specification values and the picture of developed HLS transmitter operation in the testing environment. The application field of this paper result is that the man who works in the emergency situation take HLS and transmit the live emergency situation to the head quarter using this portable HLS.

• Journal of the Korean Society of Safety
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• v.30 no.4
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• pp.159-167
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• 2015

The main purpose of this study is to evaluate the awareness survey on the experience of fire fighting safety education and living safety consciousness of college students in the department of noncommissioned officer. Additionally, making provision of basic references and the effective fire fighting safety education is another important purpose of this study. In order to evaluate the awareness, the survey consist of 43 queries based on 6 queries for general characters, 9 queries for experiences of fire fighting safety education and 30 queries for living safety consciousness. The collected data from survey was analyzed using SPSS 20.0 win program. The interrelationship for general characters was confirmed using analysis of frequency, percentage, independent T-tset, ANONVA analysis, and regression analysis. The results show that the experience of fire fighting safety education and living safety consciousness of students from the combatant branch and ordnance corps are higher than those students from quartermaster corps. This may be attributed to the presence or absence of safety relevant courses on a curriculum. Therefore, the presence of safety courses on a curriculum should be one of important points for improving the experience of fire fighting safety education and living safety consciousness of these college students.

• Journal of the Korean Society of Propulsion Engineers
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• v.20 no.3
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• pp.32-36
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• 2016

Test equipment was designed and manufactured to evaluate thermal reaction characteristic of internal insulators of solid rocket motor. Test is allowed up to chamber pressure 2,500 psi, burn-time 100 s. A cross section of test sample part is quadrature, and various test samples can be comparable at the same time. Inner temperature of test sample can be measured by thermocouples during burning. Test was executed in condition of efficient average chamber pressure 1,000 psi, efficient burn-time 10 s and safety of equipment was confirmed. Basic data for understanding thermal characteristics of internal insulator, that is, pressure-time curve, temperature-time curve in the test sample, and thermal destruction thickness of test sample was gained successfully.

• Journal of the Korean earth science society
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• v.39 no.2
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• pp.154-163
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• 2018

Geoacoustic model comprises physical and acoustic properties of submarine bottom layers influencing sound transmission through sea water and underwater. This study suggested for the first time that we made a geoacoustic model of long-coring bottom layers at the SSDP-105 drilling site of the Ulsan coastal area, which is located in the southwestern inner shelf of the East Sea. The geoacoustic model of 52 m depth below seafloor with three-layer geoacoustic units was reconstructed in the coastal sedimentary strata at 79 m in water depth. The geoacoustic model was based on the data of a deep-drilled sediment core of SSDP-105 and sparker seismic profiles in the study area. For actual modeling, the geoacoustic property values of the models were compensated to in situ depth values below the sea floor using the Hamilton modeling method. We suggest that the geoacoustic model be used for geoacoustic and underwater acoustic experiments of mid- and low-frequency reflecting on the deep bottom layers in the Ulsan coastal area of the East Sea.