• Biomedical Science Letters
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• v.27 no.2
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• pp.45-50
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• 2021

Coagulase-negative staphylococci (CoNS) are a typical group of microorganisms, and the recent advances in laboratory technology and medicine has dramatically modified their significance in medical practice. CoNS, which were previously classified as normal bacterial flora, have recently been reported to be associated with serious infectious diseases, such as surgical wound infection or periprosthetic joint infection. Representative CoNS include Staphylococcus epidermidis, S. haemolyticus, and S. saprophyticus, which are known to cause serious problems in biomaterial-based and prosthetic device infections, as well as to cause simple urinary tract infections in sexually active women. Over the last decade, the clinical isolation rate of CoNS has been increasing, and antibiotic resistance has also been occurring. This review aimed to investigate the incidence of CoNS infection and to use the results as basic data for the management of CoNS, with a focus on the isolation rate and antibiotic resistance in clinical surgery.

• The Plant Pathology Journal
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• v.27 no.4
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• pp.310-314
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• 2011

Nonstructural protein 3 (NS3) encoded by RNA3 of Rice stripe virus (RSV), known to be a suppressor of gene silencing, was cloned and sequenced. The cloned NS3 gene is composed of 636 nucleotides encoding 211 deduced amino acids, and showed a high degree of similarity with the equivalent genes isolated from Korea, Japan and China. The NS3 gene promoted the enhancement of transient gene expression and suppressed transgene co-silencing. In the transient GFP expression via agroinfiltration, GFP expression was dramatically enhanced in terms of both protein yield and expression period in the presence of NS3. The highest accumulation of GFP protein reached to 6.8% of total soluble proteins, which corresponded to a two-fold increase compared to that obtained in the absence of NS3. In addition, NS3 significantly suppressed the initiation of GFP co-silencing induced by the additive GFP infiltration in GFP-transgenic Nicotiana benthamiana. The NS3 gene was also found to be a stronger suppressor than Cucumber mosaic virus 2b. These observations are believed to be derived from the strong suppressive effect of NS3 on gene silencing, and indicate that NS3 could be used as an effective enhancer for the rapid production of foreign proteins in plants.

• Journal of IKEEE
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• v.20 no.3
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• pp.295-298
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• 2016

In this paper, we analysed the properties of the conventional ESD protection devices such as SCR, MVSCR, LVTSCR. The electrical characteristics and the turn-on time properties are simulated by Synopsys T-CAD simulator. As the results, the devices have the holding voltages between 2V and 3V, and the trigger voltage of about 20V with SCR, of about 12V with MVSCR, of about 9V with LVTSCR. The results of the simulation for the turn-on time properties are 2.8ns of SCR, 2.2ns of MVSCR, 2.0ns of LVTSCR. Thus, we prove that LVTSCR has the shortest turn-on time. However, the second breakdown currents(It2) of the devices are 7.7A of SCR, 5.5A of MVSCR, 4A of LVTSCR. This different properties have to be adapted by the operation voltages for I/O Clamps.

• Korean Journal of Optics and Photonics
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• v.6 no.1
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• pp.26-32
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• 1995

A Kerr cell was utilized as an optical shutter to generate a shortened pulse and as an isolator between amplifiers in an iodine laser system. By rotating the polarization of incident laser pulse only during the timing window of high voltage applied to the Kerr cell, shortened pulses of 5 ns and 1 ns, corresponding to the difference in propagation time of two coaxial cables, were obtained. It was also noticed that more than one timing window of Kerr cell was produced with a long incident laser pulse from the oscillator. The measured transmittance of Kerr cell with respect to applied voltage was compared with the theoretical estimation using the electro-optic Kerr effect theory. Through the amplification of the shortened pulse in iodine amplifiers. a pulse of 0.5 GW(2 J in 4 ns) was obtained. ained.

• Journal of Life Science
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• v.29 no.4
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• pp.436-441
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• 2019

Nelumbo nucifera, also known as sacred lotus, has mainly been used as a food throughout the Asian countries. In the present study, we prepared ethanol extracts from leaf (NL), seed (NS), and seedpod (NSP) of Nelumbo nucifera and investigated their anti-inflammatory activities in mouse macrophage RAW 264.7 cells. To evaluate the anti-inflammatory activities of NL, NS, and NSP, nitric oxide (NO) production was measured in LPS-stimulated RAW 264.7 cells. NL, NS, and NSP significantly reduced NO production in a dose-dependent manner without affecting cell viabilities. NL, NS, and NSP dramatically decreased the protein expression of pro-inflammatory genes such as iNOS and COX-2. NL, NS, and NSP also suppressed phosphorylation of MAPKs and the nuclear translocation of $NF-{\kappa}B$ p65 indicating they have their anti-inflammatory activities via regulating mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B ($NF-{\kappa}B$) pathways. In addition, we analyzed the production of reactive oxygen species (ROS) by the treatment of NL, NS, and NSP. All extracts reduced ROS production in a dose-dependent manner. And also, they increased heme oxygenase-1 (HO-1) protein expression and the nuclear translocation of nuclear respiratory factor 2 (Nrf2). In conclusion, our results suggest that Nelumbo nucifera has its anti-inflammatory activity via regulating MAPKs, $NF-{\kappa}B$, and Nrf2/HO-1 pathways.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.5
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• pp.162-167
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• 2009

In this paper, we present a compact Ultra-Wideband band-pass filter using a high-pass filter and low-pass filter. The structure of our proposed band-pass filter is very simple and the DGS(Defected Ground Structure) structure is used to get the low-pass filter characteristic. Our proposed band-pass filter can be much smaller than a cascaded filter. As a result of measurement the insertion loss is less than 0.5dB throughout the pass-band of $2.1GHz{\sim}10.56GHz$, the return loss is more than 20dB and the group delay maximum variation is 0.23ns from 0.12ns to 0.35ns.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.7A
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• pp.666-673
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• 2006

In this paper, we evaluated the performance of 4 types of ARQ algorithms that have been adopted to offer reliable transmission between MAC layers. First of all, we implemented IEEE 802.16 ARQ algorithms with NS-2 network simulator and then evaluated the performances in various channel states. We used two performance measures to evaluate those performances. First, we used the total number of ARQ block in bytes that have been received without error. Second, we used ACK_Efficiency that is newly defined in this paper. From the results of simulation, we could be aware that each ARQ algorithm has different performance in a various channel states. For improving the transmission data rate in a specific channel state, we have to choose the right ACK interval as well as a suitable ARQ algorithm.

• Journal of information and communication convergence engineering
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• v.10 no.4
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• pp.359-364
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• 2012

Harvesting energy from the environment is essential for many applications to slow down the deterioration of energy of the devices in sensor networks and in general, the network itself. Energy from the environment is an inexhaustible supply which, if properly managed and harvested from the sources, can allow the system to last for a longer period - more than the expected lifetime at the time of deployment, or even last indefinitely. The goal of this study is to develop a simple algorithm for ns-2 to simulate energy harvesting in wireless sensor network simulations. The algorithm is implemented in the energy module of the simulator. Energy harvesting algorithms have not yet been developed for ns-2. This study will greatly contribute to the existing knowledge of simulating wireless sensor networks with energy harvesting capabilities in ns-2. This paper will also serve as a basis for future research papers that make use of energy harvesting.

• Childhood Kidney Diseases
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• v.19 no.2
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• pp.171-175
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• 2015

Nephrotic syndrome (NS) is a common chronic disease in children; in 90 percent of cases, the condition is primary (idiopathic). Toxic nephropathy can be induced by herbal medicines, and is mainly manifested as tubulointerstitial nephritis and rarely, as glomerulopathy. Here in, we describe two cases of steroid-sensitive NS, which developed after the patients received herbal medicines. A 5-year-old boy and an 8-year-old girl were separately admitted within a short time period with acute onset of generalized edema, proteinuria, hypoalbuminemia, and hypercholesterolemia. Each patient had previously taken herbal medicine, which had been prescribed by different oriental medical clinics for different conditions. The patients were diagnosed with herbal medicine-induced NS and were treated empirically by a standard steroid therapy, with subsequent resolution of their NS. One patient relapsed, but her NS again responded to steroid therapy. We described two unusual cases of prototypical pediatric, steroid-sensitive NS, which was presumed to be minimal-change disease that developed after the administration of herbal medicines. We also reviewed the literature.

• Bulletin of the Korean Chemical Society
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• v.26 no.2
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• pp.285-291
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• 2005

The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is the viral RNA-dependent RNA polymerase (RdRp), which is the essential catalytic enzyme for the viral replication and is an appealing target for the development of new therapeutic agents against HCV infection. A small amount of serum from a single patient with hepatitis C was used to get the genome of a Korean HCV isolate. Sequence analysis of NS5B 1701 nucleotides showed the genotype of a Korean isolate to be subtype 1b. The soluble recombinant HCV NS5B polymerase lacking the C-terminal 24 amino acids was expressed and purified to homogeneity. With the highly purified NS5B protein, we established in vitro systems for RdRp activity to identify potential polymerase inhibitors. The rhodanine family compounds were found to be potent and specific inhibitors of NS5B from high throughput screening (HTS) assay utilizing the scintillation proximity assay (SPA) system. The binding mode of an inhibitor was analyzed by measuring various kinetic parameters. Lineweaver-Burk plots of the inhibitor suggested it binds not to the active site of NS5B polymerase, but to an allosteric site of the enzyme. The activity of NS5B in in vitro polymerase reactions with homopolymeric RNA requires interaction with multiple substrates that include a template/primer and ribonucleotide triphosphate. Steady-state kinetic parameter, such as Km, was determined for the ribonucleotide triphosphate. One of compounds found interacts directly with the viral polymerase and inhibits RNA synthesis in a manner noncompetitively with respect to UTP. Furthermore, we also investigated the ability of the compound to inhibit NS5B-directed viral RNA replication using the Huh7 cell-based HCV replicon system. The investigation is potentially very useful for the utility of such compounds as anti-hepatitic agents.