• Journal of Applied Biological Chemistry
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• v.59 no.2
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• pp.103-106
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• 2016

The leaves of eggplant (Solanum melongena L.) were extracted with 80 % aqueous MeOH, and the concentrated extract was partitioned with n-hexane, EtOAc, n-BuOH, and water fractions. From the n-BuOH fraction, five compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies. On the basis of physic-chemical and spectroscopic data including mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance, they were identified to be caffeic acid (1), chlorogenic acid (2), cryptochlorogenic acid (3), panasenoside (4), and (6R,7E,9R)-4,7-megastigmadien-3-one-9-${\beta}$-${\small{D}}$-glucopyranoside (5). Compounds 3 and 4 were isolated for the first time from the leaves of S. melongena L. in this study.

• Korean Journal of Pharmacognosy
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• v.7 no.3
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• pp.195-198
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• 1976

Esculin, esculetin-6-glucoside, m.p. $206^{\circ}C$ was isolated from the fresh bark ok Fraxinus chiisanensis $N_{AKAI}$, one of the indigenous plant of the oleaceous family in Korea, in yield 0.09%.

• Korean Journal of Pharmacognosy
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• v.24 no.1
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• pp.32-37
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• 1993

Phytochemical studies of the root of Aristolochia contorta afforded an unidentified N-glycoside rarely found in natural products. It's structure was elucidated as 8-desmethoxy-aristolactam $N-{\beta}-D-glucopyranoside$ by spectral data and chemical analysis. 6-Hydroxy-8-desmethoxy-aristolactam $N-{\beta}-D-glucopyranoside$, aristolactam I and aristolactam AII were also isolated from the same source. $^{13}C-NMR$ spectra were first assigned and the result confirmed N-C-O-glycosidic linkages in the glycosides.

• Applied Biological Chemistry
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• v.40 no.2
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• pp.163-166
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• 1997

A yeast, Torulopsis bombicola, was cultured in the media fortified with soybean oil as a additional carbon source for 7 days with reciprocal shaking. From the culture broth, sophorose-lipid was isolated and treated with alkali to afford sophorose. The sophorose contained in the medium was acetylated and isolated through silica gel column chromatography. The aceylated sophorose was hydrolyzed with 5% KOH at room temperature to give rise to sophorose. Meanwhile, the MeOH extracts obtained from the pod of Sophora japonica was solvent-fractionated with n-BuOH and $H_2O$, and butanolic layer was chromatographed on silica gel column to afford a flavonoide-glycoside. The glycoside was hydrolyzed with 0.02 N $H_2SO_4$ to yield sophorose.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.242-247
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• 1997

A flavone glycoside was separated from the aerial part of Circium japonicum var ussuriense Kitamura and the effect on cardiovascular system was investigated. The cadiovascular contractility of this compound was observed in the artria and aortae from normal rats. It increased the spontancous beat in right atria and the contractile force in left atria, and caused the contraction of thoracic aortae. For the blood pressure, it had ascending effect. The effective component, which acted on stimulation and contraction in the artria and aortae of rats was fractionated with n-BuOH, separated and identified by column chromatography, UV, IR, $^1H-NMR$ and $^{13}C-NMR$. The chemical structure for this component was determined to be $hispidulin-7-{\alpha}-rhamnopyranosyl(1{\rightarrow}2)-{\beta}-D-glucopyranoside$.

• Korean Journal of Pharmacognosy
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• v.22 no.4
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• pp.219-224
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• 1991

Liver protective effects of ginsenosides as well as fractions of dammarane glycosides of Panax ginseng were studied using galactosamine (GalN)-induced cytotoxicity in primary cultured rat hepatocytes. Preventing effects on GalN-induced hepatotoxicity were found both microscopic observation and determination of GPT level with total dammarane glycosides fraction and $20(S)-ginsenoside-Rb_1$ as well as $20(S)-ginsenoside-Rg_1$ at the concentration of $50{\mu}g/ml$. The syntheses of both protein and RNA were significantly increased by the treatment of $50{\mu}g/ml$ of total dammarane glycoside fraction, $20(S)-ginsenoside-Rb_1$, -Rc, -Re and $-Rg_1$, respectively in both normal and GalN-induced cytotoxic hepatocytes.

• Korean Journal of Pharmacognosy
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• v.46 no.2
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• pp.99-104
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• 2015

Three triterpenoids, one sterol glycoside and a phenylpropanoid glycoside were isolated from the n-BuOH soulble fraction of Paulownia coreana twigs. On the basis of spectral data, the structure of isolated compounds were identified as pomolic acid (1), euscaphic acid (2), arjunic acid (3), daucosterol (4), and syringin (5), respectively. All compounds are isolated from this plant for the first time.

• Archives of Pharmacal Research
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• v.26 no.2
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• pp.132-137
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• 2003

Three cerebrosides 2, 3, and 5 and two terpene glycosides 1 and 4 have been isolated from the methanol extract of the root of Aster scaber. Their structures were determined as 3-Ο-$\beta$-D-glucuronopyranosyl-oleanolic acid methyl ester (1), (2S, 3S, 4R, 2 R, 8Z, 15 Z)-N-2 -hydroxy-15 -tetracosenoyl-1-Ο-$\beta$-D-glucopyranosyl-4-hydroxy-8-sphingenine (2), (2S, 3S, 4R, 8Z)-N-octadecanoyl-1-Ο-$\beta$-D-glucopyranosyl-4-hydroxy-8-sphingenine (3), 1$\alpha$-hydroxy-6$\beta$-Ο-$\beta$-D-glucosyl-eudesm-3-ene (4), and (2S, 3S, 4R, 2 R, 8Z)-N-2 -hydroxy-hexadecanoyl-1-Ο-$\beta$-D-glucopyranosyl-4-hydroxy-8-sphingenine (5) on the basis of spectroscopic methods.

• Applied Biological Chemistry
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• v.42 no.2
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• pp.166-169
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• 1999

The repeated silica gel colum chromatographies of n-BuOH fraction obtained from MeOH extracts of Eucommia ulmoides leaves led to isolation of a flavonoid-glycoside inhibiting monoamine oxidase B activity. Its chemical structure was determined to be $3-O-[{\beta}-D-glucopyranosyl\;(1{\rightarrow}2)\;{\beta}-D-xylopyranosyl]$ quercetin through interpretation of spectral data and adaptation of acid hydrolysis. The $IC_{50}$ value of this compound in rat brain mitochondrial MAO-B inhibitory activity was evaluated to be $8.05\;{\mu}mol/l$.

• Applied Biological Chemistry
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• v.40 no.6
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• pp.583-587
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• 1997

The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and $H_2O$, respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, ${\beta}-sitosterol$, and two kinds of unsaturated fatty acids.