Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of Monoamine Oxidase B Inhibitory Compound from the Leaves of Eucommia ulmoides Oliv.

두충(Eucommia ulmoides Oliv.)잎으로부터 Monoamine Oxidase B 억제활성물질의 분리

  • Ahn, Eun-Mi (Department of Life Sciences & Institute of Life Sciences, Kyung Hee University) ;
  • Hahn, Jae-Taek (Department of Life Sciences & Institute of Life Sciences, Kyung Hee University) ;
  • Lee, Dong-Wook (Korea Ginseng & Tabacco Research Institute) ;
  • Sohn, Hyung-Ok (Korea Ginseng & Tabacco Research Institute) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Baek, Nam-In (Department of Life Sciences & Institute of Life Sciences, Kyung Hee University)
  • 안은미 (경희대학교 생명과학부 및 생명과학연구원) ;
  • 한재택 (경희대학교 생명과학부 및 생명과학연구원) ;
  • 이동욱 (한국인삼연초연구원) ;
  • 손형옥 (한국인삼연초연구원) ;
  • 권병목 (한국과학기술연구원 생명공학연구소) ;
  • 백남인 (경희대학교 생명과학부 및 생명과학연구원)
  • Published : 1999.05.31
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Abstract

The repeated silica gel colum chromatographies of n-BuOH fraction obtained from MeOH extracts of Eucommia ulmoides leaves led to isolation of a flavonoid-glycoside inhibiting monoamine oxidase B activity. Its chemical structure was determined to be $3-O-[{\beta}-D-glucopyranosyl\;(1{\rightarrow}2)\;{\beta}-D-xylopyranosyl]$ quercetin through interpretation of spectral data and adaptation of acid hydrolysis. The $IC_{50}$ value of this compound in rat brain mitochondrial MAO-B inhibitory activity was evaluated to be $8.05\;{\mu}mol/l$.

두충잎으로부터 얻은 MeOH 추출물의 n-BuOH 분획으로부터 silica gel column chromatography를 반복하여 monoamine oxidase B (MAO-B) 억제 활성을 갖는 flavonoid 배당체를 분리하였다. 화합물의 화학구조를 스펙트럼 데이터의 해석과 산가수분해반응을 이용하여 $3-O-[{\beta}-D-glucopyranosyl\;(1{\rightarrow}2)\;{\beta}-D-xylopyranosyl]\;quercetin$으로 결정하였다. 이 화합물의 MAO-B 억제활성에 있어서의 $IC_{50}$ 값은 $8.05\;{\mu}mol/l$로 측정되었다.

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