• Analytical Science and Technology
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• v.32 no.2
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• pp.35-47
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• 2019

In this study, high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to detect 26 antidiabetic compounds in adulterated dietary supplements using a simple, selective method. The work presented herein may help prevent incidents related to food adulteration and restrict the illegal food market. The best separation was obtained on a Shiseido Capcell Pak(R) C18 MG-II ($2.0mm{\times}100mm$, $3{\mu}m$), which improved the peak shape and MS detection sensitivity of the target compounds. A gradient elution system composed of 0.1 % (v/v) formic acid in distilled water and methanol at a flow rate of 0.3 mL/min for 18 min was utilized. A triple quadrupole mass spectrometer with an electrospray ionization source operated in the positive or negative mode was employed as the detector. The developed method was validated as follows: specificity was confirmed in the multiple reaction monitoring mode using the precursor and product ion pairs. For solid samples, LOD ranged from 0.16 to 20.00 ng/mL and LOQ ranged from 0.50 to 60.00 ng/mL, and for liquid samples, LOD ranged from 0.16 to 20.00 ng/mL and LOQ ranged from 0.50 to 60.00 ng/mL. Satisfactory linearity was obtained from calibration curves, with $R^2$ > 0.99. Both intra and inter-day precision were less than 13.19 %. Accuracies ranged from 80.69 to 118.81 % (intra/inter-day), with a stability of less than 14.88 %. Mean recovery was found to be 80.6-119.0 % and less than 13.4 % RSD. Using the validated method, glibenclamide and pioglitazone were simultaneously determined in one capsule at concentrations of 1.52 and 0.53 mg (per capsule), respectively.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.478-490
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• 2021

We aimed to generate basic breeding data for melon (Cucumis melo L.). A total of 219 melon accessions conserved at the National Agrobiodiversity Center (NAC) in Rural Development Administration (RDA) were used in this study, of which 72 (33%) were collected from India. The majority of accessions showed orange (42%) and white (36%) flesh color. In addition to phenotypic evaluations, the accessions were genotyped using a molecular marker for the carotenoid biosynthesis gene CmOr. DNA fragments of the expected size were amplified in 205 out of 219 accessions. Digestion of the PCR products with HinfI restriction endonuclease showed 100% concordance between phenotype and genotype in green-fleshed accessions, but 98%, 97%, and 80% concordance in orange-, white-, and creamy-fleshed accessions, respectively. Sequence analysis revealed single nucleotide changes in the three positions of SNP1, SNP2 and SNP1int in the CmOr gene among accessions. These newly found alleles suggest that there are multiple mechanisms in determining fruit flesh color in melon. Also, the phenotype data of diverse accessions obtained in this study will be a valuable source for melon breeding.

• Journal of Life Science
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• v.31 no.8
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• pp.778-785
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• 2021

The germination of cucumber seeds begins with the degradation of reserved oil to fatty acids within the lipid body, which are then further metabolized to acyl-CoA. The acyl-CoA moves from the lipid body to the glyoxysome following β-oxidation for the production of acetyl-CoA. As an initial carbon source supplier, acetyl-CoA is an essential molecule in the glyoxylate cycle within the glyoxysome, which produces the metabolic intermediates of citrate and malate, among others. The glyoxylate cycle is a necessary metabolic pathway for oil seed plant germination because it produces the metabolic intermediates for the tricarboxylic acid (TCA) cycle and for gluconeogenesis, such as the oxaloacetate, which moves to the cytosol for the initiation of gluconeogenesis by phophoenolpyruvate carboxykinase (PEPCK). Following reserved oil mobilization, the production and transport of various metabolic intermediates are involved in the coordinated operation and activation of multiple metabolic pathways to supply directly usable carbohydrate in the form of glucose. Furthermore, corresponding gene expression regulation compatibly transforms the microbody to glyoxysome, which contains the organelle-specific malate synthase (MS) and isocitrate lyase (ICL) enzymes during oil seed germination. Together with glyoxylate cycle, carnitine, which mediates the supplementary route of the acetyl-CoA transport mechanism via the mitochondrial BOU (A BOUT DE SOUFFLE) system, possibly plays a secondary role in lipid metabolism for enhanced plant development.

• Economic and Environmental Geology
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• v.54 no.4
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• pp.475-482
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• 2021

This study proposed a strategy with literature review on effective monitoring of dispersion of the particulate matters (PM) emitted from domestic open pit lime mines. The mines generally produced a large amount of PM through the mine processes such as crushing and transportation of raw or crushed ores. The PM emission from mine facilities or transportation can be assessed using empirical equations which was prepared through the experimental tests to produce PM from ores. For effective monitoring of mine PM dispersion, this study showed a preliminary application of the monitoring network with multiple low-cost sensors around a main PM emission source for each mine site. Therefore, two domestic limestone mine sites were selected for this study, and install the monitoring network with low-cost PM sensors and LTE (Long-term evolution) data communication. Then, preliminary resultant PM data plotted according to monitoring duration showed typical PM dispersion patterns. The quantification of the PM dispersion patterns should be roughly prepared by a PM size-dependent dispersion modeling.

• Journal of Life Science
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• v.32 no.7
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• pp.550-566
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• 2022

Stroke is among the leading causes of death and long-term physical and cognitive disabilities worldwide, affecting an estimated 15 million people annually. The pathophysiological process of stroke is complicated by multiple and coordinated events. The breakdown of the blood-brain barrier (BBB) in people with stroke can significantly contribute to the development of ischemic brain injury. Therefore, BBB disruption is recognized as a hallmark of stroke; thus, it is important to develop novel therapeutic strategies that can protect against BBB dysfunction in ischemic stroke. Traditional medicines are composed of natural products, which represent a promising source of new ingredients for the development of conventional medicines. Indeed, several studies have shown the effectiveness of Korean medicine on stroke, highlighting the value of Korean medicinal treatment for ischemic stroke. This review summarizes the current information and underlying mechanisms regarding the ameliorating effects of the formula, decoction, herbs, and active components of traditional Korean medicine on cerebral ischemia-induced BBB disruption. These traditional medicines were shown to have protective effects on the BBB in many cellular and animal ischemia models of stroke, and experiments in various animal species, such as mice and rats. In addition, they showed brain-protective effects by protecting the BBB through the regulation of tight junction proteins and matrix metalloproteinase-9, reducing edema, neuroinflammation, and neuronal cell death. We hope that this review will help promote further investigation into the neuroprotective effects of traditional Korean medicines and stimulate the performance of clinical trials on Korean herbal medicine-derived drugs in patients with stroke.

• Applied Chemistry for Engineering
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• v.33 no.6
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• pp.563-573
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• 2022

This work aimed to develop a new equation for turbidity (Turb) simulation and prediction using statistical methods based on principal component analysis (PCA) and multiple linear regression (MLR). For this purpose, water samples were collected monthly over a five year period from Cheurfa dam, an important reservoir in Northwestern Algeria, and analyzed for 12 parameters, including temperature (T°), pH, electrical conductivity (EC), turbidity (Turb), dissolved oxygen (DO), ammonium (NH₄⁺), nitrate (NO₃^-), nitrite (NO₂^-), phosphate (PO₄^3-), total suspended solids (TSS), biochemical oxygen demand (BOD₅) and chemical oxygen demand (COD). The results revealed a strong mineralization of the water and low dissolved oxygen (DO) content during the summer period. High levels of TSS and Turb were recorded during rainy periods. In addition, water was charged with phosphate (PO₄^3-) in the whole period of study. The PCA results revealed ten factors, three of which were significant (eigenvalues >1) and explained 75.5% of the total variance. The F1 and F2 factors explained 36.5% and 26.7% of the total variance, respectively and indicated anthropogenic pollution of domestic agricultural and industrial origin. The MLR turbidity simulation model exhibited a high coefficient of determination (R² = 92.20%), indicating that 92.20% of the data variability can be explained by the model. TSS, DO, EC, NO₃^-, NO₂^-, and COD were the most significant contributing parameters (p values << 0.05) in turbidity prediction. The present study can help with decision-making on the management and monitoring of the water quality of the dam, which is the primary source of drinking water in this region.

• Korean Journal of Community Nutrition
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• v.26 no.6
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• pp.495-507
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• 2021

Objectives: This study examined regional differences in the intake of dietary total fat and saturated fatty acid (SFA) and their food sources among Korean adults. We also investigated the associations of SFA intake with metabolic diseases by region. Methods: This study included 13,926 adults (≥ 19y) who participated in the 2016 ~ 2019 Korea National Health and Nutrition Examination Surveys. The regions were divided into urban and rural areas according to the administrative districts where the participants lived. Using dietary data obtained from a 24-h recall, intake of total fat and SFA and their food sources were assessed by region. Metabolic diseases included obesity, abdominal obesity, and elevated total cholesterol and their association with SFA intake by region were examined using multiple logistic regression. Results: Of the participants, 19.6% lived in rural areas. In urban areas, the total fat and SFA intakes were higher than in rural areas: 21.2% of energy (%E) came from total fat and 6.9%E from SFA in urban areas, whereas 18.0%E came from total fat and 5.8%E from SFA in rural areas. The percentage of participants who exceeded the dietary reference intakes for total fat and SFA in urban areas was 16.5% and 41.9%, respectively, but 43.4% of participants in rural areas showed lower intake levels for total fat compared to the reference level. Young adults did not show regional differences in fat intake, and the percentage of subjects who exceeded the reference for SFA was high both in urban (58.5%) and rural (55.7%) areas. Among middle-aged and older adults, intake of fatty acids except for n-3 fatty acid was significantly higher in urban areas than in rural areas. About 69% of older adults in rural areas showed a lower intake of total fat compared to the reference level. The food sources for total fat and SFA were meat, soybean oil, eggs, and milk in both areas. The intake of fat from eggs, milk, mayonnaise, and bread was higher in urban areas, but the intake of fat from white rice and coffee mix was higher in rural areas. The SFA intake was positively associated with elevated serum total cholesterol in urban areas (4th quartile vs. 1st quartile, OR: 1.22, 95% CI: 1.06-1.40, P for trend: 0.043), but not in rural areas. Conclusions: Regional differences in total fat and SFA intakes and their food sources were observed among Korean adults. Our findings may help plan nutritional strategies to ameliorate regional health disparities.

• Geophysics and Geophysical Exploration
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• v.25 no.1
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• pp.26-37
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• 2022

Recently, multiple methods using small aircraft for geophysical exploration have been suggested as a result of the development of information and communication technology. In this study, we introduce the hybrid unmanned aircraft electromagnetic system of the Korea Institute of Geosciences and Mineral resources, which is under development. Additionally, data processing and interpretation methods are suggested via the analysis of datasets obtained using the system under development to verify the system. Because the system uses a three-component receiver hanging from a drone, the effects of rotation on the obtained data are significant and were therefore corrected using a rotation matrix. During the survey, the heights of the source and the receiver and their offsets vary in real time and the measured data are contaminated with noise. The noise makes it difficult to interpret the data using the conventional method. Therefore, we developed a recurrent neural network (RNN) model to enable rapid predictions of the apparent resistivity using magnetic field data. Field data noise is included in the training datasets of the RNN model to improve its performance on noise-contaminated field data. Compared with the results of the electrical resistivity survey, the trained RNN model predicted similar apparent resistivities for the test field dataset.

• The Korean Journal of Franchise Management
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• v.13 no.3
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• pp.1-15
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• 2022

Purpose: In the restaurant sector, it has been known that consumers' positive perception of brands influences their positive WOM intention, and information sources play an important role in increasing credibility by enhancing consumer awareness and developing differentiated brands. This study examines the effects of information sources (e.g., advertisement, WOM, SNS) on trust (cognitive and affective) and, WOM and eWOM intention in the restaurant context. In the model, cognitive and affective trust play mediating roles in the relationships between information sources (e.g., advertisement, WOM, SNS) WOM and eWOM intention. Research design, data, and methodology: Research models and hypotheses were developed according to the research direction. The survey questionnaire items were developed and used appropriately according to the contents of this paper based on prior studies. All constructs were measured with multiple items developed and validated in prior studies. A total of 502 responses were collected from an online survey. The research model was evaluated using SmartPLS 4.0. Frequency analysis was performed to understand the demographic characteristics of the survey respondents. The reliability, convergent validity, and discriminant validity were assessed using measurement model analysis. The proposed model was verified using the structural equation model. Results: Advertisement, WOM, and SNS information sources all had a positive effect on affective trust, whereas only WOM had a significant effect on cognitive trust. In addition, affective trust had a positive effect on cognitive trust and eWOM intention but did not affect WOM intention. Finally, cognitive trust was found to have a positive effect on both WOM intention and eWOM intention. Conclusions: This study redefines the concept of where restaurant service companies should focus when providing consumers with information about their products and services. As a result, the conceptual framework of positive word of mouth intention to increase new customer visits to the restaurant brand has been expanded. In addition, this study not only presents an information source management strategy for restaurant brands, but also presents practical implications for resource allocation guidelines for customer management in the restaurant sector.

• Journal of the Korean Society for Library and Information Science
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• v.57 no.3
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• pp.27-48
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• 2023

This study aims to investigate the impact of job stress on the job satisfaction and life satisfaction of local government officials who participated in the reading academy from January 2023 to March 2023. The research involved collecting 300 questionnaires and conducting multiple regression analysis to analyze the obtained data. The results of the study are presented below. Impact of Job Stress on Job Satisfaction: The analysis revealed that among the sub-factors of job stress, inappropriate compensation significantly increases job stress among local government officials in the reading academy. Moreover, the study found that turnover intention was particularly high when compensation was not recognized as appropriate, indicating it as the most significant source of job stress. Impact of Job Stress on Life Satisfaction: The findings demonstrate that several factors negatively influence job stress, leading to reduced life satisfaction among local government officials in the reading academy. These factors include undesirable changes in working conditions or situations, anxiety about reorganization, inconsistent work orders, gender discrimination, and an authoritative organizational atmosphere. Effect of Job Satisfaction on Life Satisfaction: The analysis of the relationship between job satisfaction and life satisfaction indicated that turnover intention and remuneration level in job satisfaction positively influenced emotional satisfaction. Conversely, the job itself and interpersonal relationships had a significant negative effect on emotional satisfaction. Furthermore, the job itself and the level of remuneration in job satisfaction showed a significant positive effect on physical satisfaction. Similarly, the job itself and the level of remuneration in job satisfaction positively affected social satisfaction, while the intention to change jobs had a significant negative impact.