Objectives : In this study, the anti-stress effects of ethanolic extract of Opuntiaficus-indica (OF70E) were investigated. Methods : To determine the effects of OF70E on physical stress, changes in whole-body cortisol level or behavior were monitored in zebrafish. After treatment with 0.9% saline or OF70E for 6 min, all fish underwent net handling stress (NHS), which induced physical stress. And then, we conducted open field test (OFT) or sacrificed fish for collecting body fluid from whole-body. We used the cortisol enzyme-linked immunoassay kit to measure the amount of cortisol in each zebrafish sample. Results : In result, compared with normal group which were not treated by NHS, whole-body cortisol levels were significantly increased in stressed-control group. Compared with control group, pretreatment with OF70E at concentrations of 25, 50 and 100 mg/L for 6min significantly inhibited the increase of whole-body cortisol levels induced by NHS(p<0.05). To anti-stress effects of OF70E on behavior, we conducted OFT after the induction of NHS following pretreatment of OF70E. As results of OFT, compared with unstressed-normal group, distance moved was significantly decreased by induction of NHS in stressed-control group (p<0.05). OF70E-pretreatment blocked decreases of distance moved increased by NHS (p<0.05). And meandering movement, immobility and turn angle were significantly increased by NHS in stressed-control group compared with unstressed-normal group (p<0.05). OF70E-pretreatment prevented the increases of meandering movement immobility and turn angle by NHS (p<0.05). Conclusions : In conclusion these results suggest that OF70E-pretreatment may prevent stress responses.
This study investigated external symptoms, behavior characteristics and the distribution pattern of the scuticociliates in the skin, gill and blood vessel of the tiger puffer, Takifugu rubripes, infected with scuticociliates among tiger puffer cultured. The fish infected with scuticociliates did not show any external symptoms, such as change in body color or swimming behavior, in the early infection stage. However, they showed congestion, erosion, and ulcer on the skin and fin at advanced stages. They showed a turning movement, mainly stayed on the bottom, and swam with their mouths open at the surface of water. Some fish showed a sudden swimming movement of zigzag type. The scuticociliates were ovoid in shape and $20\times40{\mu}m$ in size. The scuticociliates had a contractile vesicle and a caudal cilium that ranged 10-12 ${\mu}m$, and reproduced by binary fission. Therefore the scuticociliates were identified as ciliated, belonging to class Hymenostomatia, order Scuticociliatida in morphogenetic character. The gills infiltrated with scuticociliates showed clubbed gill filament due to hypertrophy of gill lamella. Within the blood vessels, scuticociliates were observed one to ten individuals, depending on diameter of the blood vessels. Some of the scuticociliates were observed to have ingested erythrocytes.
Journal of the Korean Society of Marine Environment & Safety
/
v.23
no.2
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pp.139-145
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2017
Recently, gusts, typhoon and tsunamis have been occurring more frequently around the world. In such an emergency situation, a moored vessel can be used to predict and analyze other vessel behavior, but if the mooring system is destroyed, marine casualties can occur. Therefore, it is necessary to determine quantitatively whether a vessel should be kept in the harbour or evacuate. In this study, moored ship safety in an exclusive wharf according to swell effects on motion and mooring load have been investigated using numerical simulations. The maximum tension exerted on mooring lines exceeded the Safety Working Load for intervals 12 and 15 seconds. The maximum bollard force also exceeded 35 tons (allowable force) in all evaluation cases. The surge motion criteria result for safe working conditions exceeded 3 meters more than the wave period 12 seconds with a wind speed of 25 knots. As a result, a risk rating matrix (risk category- very high risk, high risk and moderate risk) was developed with reference to major external forces such as wind force, wave height and wave periods to provide criteria for determining the control of capabilities of mooring systems to prevent accidents.
In light of the world grappling with diverse environmental issues due to climate change, the prominence of the 'pre-cycling' movement has grown. This movement strives to curtail or eliminate waste generation even before product purchases, garnering more attention than conventional eco-friendly practices. Consequently, this study employs experiments to delve into effective message strategies for promoting pre-cycling behaviors, which serve as preventive measures against the escalating environmental challenges posed by worsening waste problems. This study investigated the effects of message framing and temporal distance on pre-cycling behavioral intention. In terms of message frame, suggestive messages increased pre-cycling behavioral intention more than coercive messages, and temporal distance had no direct effect on pre-cycling behavioral intention. Assertive messages were associated with higher pre-cycling behavioral intentions when the target was interpreted as near future and suggestive messages were interpreted as distant future. Although temporal distance did not directly affect pre-cycling behavioral intention, it positively influenced consumers' pro-environmental behavioral intention by reducing their antipathy toward assertive messages. The implications of this study extend to the realm of management, offering insights into steering consumer behavior in response to pro-environmental messages.
Based on theoretical background of Situational Theory of Problem Solving(STOPS), we have examined the role of STOPS variables on a group of online public in their issue recognition and communicative behavior on Korea's nationwide civil boycott movement of Japanese goods. Results from 524 survey cases from a Korea's major online community show that two independent variables(Problem Recognition, Referent Criterion) revealed positive in their effect on mediating variable(Situational Motivation in Problem Solving). Situational Motivation also revealed positive in its effect on six dependent variables(Information Forefending, Information Permitting, Information Forwarding, Information Sharing, Information Seeking, Information Attending) of Communicative Behavior. Involvement Recognition and Constraint Recognition revealed positive without proper statistical significance. As a result, study on the case of online public in Korea supports STOPS theory as high-level of Problem Recognition and Referent Criterion effects on Communicative Behavior in positive way via Situational Motivation. Implications from the findings have discussed and proposed suggestions for government public relations and further studies.
Proceedings of the Korean Geotechical Society Conference
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1999.10a
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pp.449-456
/
1999
The concrete wall is the most useful of retaining structure which can obtain the engineering stability, but has problems that is not friendly with nature environment in a fine view, such as poor rear drainage, and shrinkage crack by temperature difference, etc. Because of this problems, the research for a segmental crib retaining wall has been performed. A segmental crib retaining wall is quickly and easily erected because is possible to be erected as the individual members, and is not sensitive to differential settlement and earthquakes. Also, it shows effective drainage and has a friendly advantage with nature environment because of being able to be planted with vines and shrubs in retaining walls The design of crib retaining walls has traditionally been based on classical soil mechanics theories. These theories, originally derived by Rankine(1857) and Coulomb(1776), assume that the wall acts as a rigid body. This assumption results in failure being predicted by either monolithic overturning or base sliding mechanisms. However, the wall consists of individual members which have been created a three dimensional grid. This grid confines an fill mass which becomes part of the wall. The filled wall resists the earth pressure with the same mechanism of classical gravity walls. Because of the flexibility of the individual segment, it allows relative movement between the individual members within the wall. The three dimensional flexible grid leads to stress redistribution when the wall is subjected to external or fill loads. Due to the flexibility and the stress redistribution, the failure of segmental crib wall consists of not only overturing and base sliding but the local deformation and the failure between the segmental members. It has been researched in the field that due to this flexibility and load redistribution, serviceability failure of segmental crib walls is unlikely to be due to overturning or base sliding. Therefore, in this study, the relative displacement appearance of retaining wall due to variation of inclination is measured to examine this behavior characteristics. Also, the behavior characteristics of retaining walls by surcharge load, and location of acting point of retaining wall rear, and the displacement characteristics and deflections are estimated about the existence and nonexistence of Rear Stretcher performing an role in transmitting earth pressure of Header and Stretcher organizing retaining walls. This research focuses on the characteristics due to the behavior of retaining walls. This research focuses on the characteristics due to the behavior of retaining walls.
The ussur brown katydid, Paratlanticus ussuriensis (Orthoptera: Tettigoniidae), causes substantial damage in orchard areas in South Korea. Daily rhythm and mating behaviors were investigated in last instar nymphs, immature adults and mature adults of P. ussuriensis. The daily rhythm was repeated upon resting, moving, and feeding. The daily rhythm of the movement behavior similar in both last instar nymphs and immature adults. Last instar nymphs becoming active at 10:06 and ceasing activity at 21:12 in average. Immature adults becoming active at 11:30 and ceasing activity at 20:08 in average of totally 10 individuals. However, mature adults showed a different active time of 15:30. Generally, behavior was affected by the conditions of their environment, especially temperature. Mature adults activated later than nymphs and immature adults. Mature adults display mating behavior at a particular time of day (approximately 20:00). P. ussuriensis showed a mating behavioral sequence which was similar to related species in completely retaining the spermatophylax. Females took 270 min to 360 min to completely retain the spermatophylax. The daily rhythm of P. ussuriensis, indicating its occurrence and ecology, could be useful as basic data for managing field populations of P. ussuriensis.
Journal of the Korea Academia-Industrial cooperation Society
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v.18
no.10
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pp.739-744
/
2017
Elastic deformation and fatigue damage can cause the permanent deformation of a kart's frame during turning, affecting the kart's driving performance. A kart's frame does not contain any suspension or differential devices and, therefore, the dynamic behavior caused by torsional deformation when driving along a curve can strongly affect these two kinds of deformations. To analyze the dynamic behavior of a kart along a curved section, the GPS trajectory of the kart is obtained and the torsional stress acting on the kart-frame is measured in real time. The mechanical properties of leisure and racing karts are investigated by analyzing their material properties and conducting a tensile test. The torsional stress concentration and frame distortion are investigated through a stress analysis of the frame on the basis of the obtained results. Leisure and racing karts are tested in each driving condition using driving analysis equipment. The behavior of a kart when being driven along a curved section is investigated through this test. Because load movement occurs owing to centrifugal force when driving along a curve, torsional stress acts on the kart's steel frame. In the case of a leisure kart, the maximum torsional stress derived from the torsional fatigue limit was found to be 230 MPa, and the torsional fatigue limit coefficient was 0.65 when driving at a speed of 40 km/h. Furthermore, the driving elements during the cornering of a kart were measured based on an actual auto-test after installing a driving measurement system, and the driving behavior of the kart was analyzed by measuring its vertical displacement.
Park, Mi-Koung;Kim, Yun-Hee;Jung, Sang-Hyuk;Beak, Kwang-Woo
Journal of The Korean Dental Society of Anesthesiology
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v.6
no.2
s.11
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pp.103-112
/
2006
Background: The purpose of this study was to compare the clinical safety and effect with and without additional submucosal midazolam to oral chloral hydrate and hydroxyzine when used for pediatric conscious sedation in a clinical dental environment. Methods: Thirty one cases of pediatric conscious sedations were performed in this study. Selection criteria included good health (ASA I), under 6 years old, 20 kg of body weight, uncooperative behavior and the need for sedation to receive dental treatment including anesthesia and restorative or surgical procedure for at least two teeth. In each visit, patients were randomly assigned into one of two groups; CH group: chloral hydrate (60 mg/kg), hydroxyzine (1 mg/kg), CH-M group: chloral hydrate (60 mg/kg). hydroxyzine (1 mg/kg) and submucoal midazolam (0.1 mg/kg). 50% nitrous oxide-oxygen was maintained during the sedation period Sedations were monitored using a pulse oximeter for estimating pulse rate (PR) and percutaneous oxygen saturation ($SpO_2$). Behavior response rated using Houpt's scale and need of restraint was assessed every 2 minutes through 30 minutes of operative procedure reviewing the videotape recording. Evaluation of overall behavior success was performed using modified overall behavior rate of Houpt's scale. Data was analyzed using t-test. Results: PR and $SpO_2$ for both groups remained within the normal values. The mean scores for sleep and movement of CH-M group were higher than those of CH group (P < 0.05). There were no significant difference in mean score for crying between two groups. The mean scores of overall behavior of CH-M group was higher than those of CH group (P < 0.01). Reinstraint of CH-M group was less required than that of CH group (P < 0.05). Conclusions: Oral chloral hydrate (60 mg/kg) and hydroxyzine (1 mg/kg) combined with submucosal injection of midazolam was safer and showed more improved sedation effect than oral chloral hydrate (60 mg/kg) and hydroxyzine (1 mg/kg) without midazolam for sedation of pediatric dental patients.
Yong-Ki Lee;Seungbeom Choi;Kyung-Woo Park;Jin-Seop Kim;Taehyun Kim
Tunnel and Underground Space
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v.33
no.1
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pp.42-56
/
2023
In deep geological disposal of high-level radioactive waste (HLW), the natural barrier must physically support the disposal facility and delay the movement of radionuclides for at least hundreds of thousands of years. To evaluate the long-term geological evolution of the natural barriers, it is essential to analyze the long-term behavior of rock joints, including the frictional healing behavior. This study aimed to experimentally analyze the frictional healing behavior of rock joints under hydro-mechanical (H-M) conditions through the slide-hold-slide (SHS) test. The SHS tests were performed under mechanical and H-M conditions for joint specimens of different roughness. In the H-M conditions, the frictional healing rate tended to increase, which was more evident in the specimens with large roughness. In addition, it was confirmed that the effect of the hydro-mechanical conditions was more significant when the effective normal stress acting on the joint surface was small. These results are expected to be used as fundamental data to understand the frictional healing behavior of rock joints in the natural barriers.
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