External Symptoms of Tiger Puffer, Takifugu rubripes Infected with Scuticociliates and Distribution of the Scuticociliates in the Skin, Gill and Blood Vessel

스쿠티카섬모충에 감염된 자주복, Takifugu rubripes 외부증상과 피부, 아가미, 혈관내 충체의 분포

  • Kang, Bub-Se (Jeju Aquacuture Place, Sajo CS Co., Ltd.) ;
  • Go, Hwan-Bong (Jeju Aquacuture Place, Sajo CS Co., Ltd.) ;
  • Kim, Sung-Jun (Marine and Environmental Research Institute, Cheju National University) ;
  • Na, Oh-Soo (Marine and Environmental Research Institute, Cheju National University) ;
  • Lee, Chi-Hoon (Marine and Environmental Research Institute, Cheju National University) ;
  • Kim, Sam-Yeon (Marine and Environmental Research Institute, Cheju National University) ;
  • Lee, Je-Hee (Faculty of Applied Marine Science, Cheju National University) ;
  • Lee, Young-Don (Marine and Environmental Research Institute, Cheju National University)
  • 강법세 (사조 CS(주) 제주양식장) ;
  • 고환봉 (사조 CS(주) 제주양식장) ;
  • 김성준 (제주대학교 해양과학연구소) ;
  • 나오수 (제주대학교 해양과학연구소) ;
  • 이치훈 (제주대학교 해양과학연구소) ;
  • 김삼연 (제주대학교 해양과학연구소) ;
  • 이제희 (제주대학교 해양과학부) ;
  • 이영돈 (제주대학교 해양과학연구소)
  • Published : 20050400

Abstract

This study investigated external symptoms, behavior characteristics and the distribution pattern of the scuticociliates in the skin, gill and blood vessel of the tiger puffer, Takifugu rubripes, infected with scuticociliates among tiger puffer cultured. The fish infected with scuticociliates did not show any external symptoms, such as change in body color or swimming behavior, in the early infection stage. However, they showed congestion, erosion, and ulcer on the skin and fin at advanced stages. They showed a turning movement, mainly stayed on the bottom, and swam with their mouths open at the surface of water. Some fish showed a sudden swimming movement of zigzag type. The scuticociliates were ovoid in shape and $20\times40{\mu}m$ in size. The scuticociliates had a contractile vesicle and a caudal cilium that ranged 10-12 ${\mu}m$, and reproduced by binary fission. Therefore the scuticociliates were identified as ciliated, belonging to class Hymenostomatia, order Scuticociliatida in morphogenetic character. The gills infiltrated with scuticociliates showed clubbed gill filament due to hypertrophy of gill lamella. Within the blood vessels, scuticociliates were observed one to ten individuals, depending on diameter of the blood vessels. Some of the scuticociliates were observed to have ingested erythrocytes.

스쿠티카섬모충이 사육중인 자주복에 침입하였을 때 감염어의 외부증상, 행동특성 및 피부, 아가미 그리고 혈관내 충체의 분포 양상을 조사하였다. 스쿠티카섬모충에 감염된 개체는 초기에 체색 변화나 유영행동 등의 외부증상을 인지할 수 없었으나, 몸체에 감염부위가 병리적인 진행에 따라 표피와 지느러미에 발적과 부식, 그리고 궤양증상을 보였다. 또한 감염어는 정상개체와 달리 무리에서 이탈하여 선회 또는 저면에 멈춰 있거나 수면 위로 입올림 하였으며, 일부 개체에서는 돌발적인 유영도 보였다. 사육중인 자주복에 침입한 스쿠티카섬모충은 타원형으로 크기가 20-40 ${\mu}m$ 범위였고, 몸체의 미부에 수축포와 10-12 ${\mu}m$되는 하나의 긴 섬모가 개재되어 있으며, 생식방법은 이분열로 증식하여 형태분류학적인 측면에서 막구충아강 채찍섬모충목의 특색을 나타내었다. 아가미에 스쿠티카섬모충이 감염된 경우에는 아가미 새판상피의 비대로 새엽이 곤봉화 되는 병리적 현상이 있었다. 혈관 속에 침입한 스쿠티카섬모충들은 혈관의 직경 크기에 따라 1-2마리에서 많게는 9-10마리씩 산재하였고, 그들 중 일부 개체에서 적혈구 세포를 섭취한 스쿠티카섬모충이 관찰되었다.

Keywords

References

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