• Korean Chemical Engineering Research
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• v.50 no.5
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• pp.874-878
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• 2012

In the submerged fermentation of fungi, it was known pH had significant effect on the citric acid production. Various growth conditions were applied with different buffer on citric acid production by Aspergillus niger NRRL 567 grown on peat moss to find the optimum pH and most effective buffer solution. The initial pHs of different buffer solutions significantly influenced on the citric acid production and A. niger NRRL 567 produced citric acid more efficiently at high pHs. A phosphate buffer and a carbonate buffer with pH 8.6 and pH 10.0 were identified as suitable buffer solutions for citric acid production. The maximal citric acid production of 564.3 g/kg solid substrate was achieved employing carbonate buffer at pH 10.0.

• Korean Journal of Environment and Ecology
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• v.8 no.1
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• pp.74-77
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• 1994

To examine the effects of gibberelin treatment on seed germination of five Viola species - Viola variegata, Viola dissecta var, chaerophylloides, Viola collina, Viola hirtipes and Viola manshrica - in Wonju district, pretreated seeds of five species were sowed on Jiffy strips with mixed soil(sand： peat moss： perlite： vermiculite, 1:1:1:1, v/v) in 25, May 1994. Seeds were soaked in 5, 25, 50ppm gibberelin solution and ground water during 24 hours. Seed germination rate was measured and compared among species and treatments. Seed germination rate of Viola hirtipes was differed higly significant among pretreatments, but those of other species did not significant at 5of level. In general GA$_3$pretreatment raised seed germination rate of 5 Viola species, and proper concentration of GA$_3$was 25~50ppm.

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.402-406
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• 2014

The present study was carried out to evaluate the potential of lignocellulosic byproducts for the production of citric acid through solid-state fermentation by Aspergillus niger NRRL 567. A sequential optimization based on one-factor-at-a-time method was applied to optimize fermentation conditions and media constituents. The results obtained from the optimization indicated that $30^{\circ}C$, 70% moisture content, 0.5~1.0 mm particle size, pH 5.5 and 4% methanol were found to be the optimum condition at 72 hr fermentation. The application the optimization resulted in an improvement of maximum citric acid production from 74.5 to 206.0 g/kg dry material (DM) from wheat straw. The optimal condition was used to produce citric acid from A. niger grown on different lignocellulosic byproducts, including wheat straw, corn stover and peat moss. A. niger produced the highest citric acid levels of 231.8, 213.8 and 240.2 g/kg DM at 120 hr fermentation, respectively.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.46-58
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• 2017

This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation and seedling growth in Microlepia strigosa (Thunb.) C. Presl. Spore germination and prothallus development were promoted by low concentrations of Knop medium nutrient solution. The optimal medium for prothallus propagation and antheridium formation was 2X MS medium with 3% sucrose. The activated charcoal content of the medium did not affect prothallus proliferation. Among the various combinations of culture soil (bedding soil, peat moss, perlite and decomposed granite), a mixture of bedding soil, peat moss and decomposed granite at a ratio of 1 : 1 : 1 (v : v : v) had a positive effect on sporophyte formation. The most efficient conditions for promoting the growth of whole plants (sporophyte seedlings) were 50 - cell plug trays filled with a mixture of bedding soil and decomposed granite at a 2 : 1 (v : v) ratio.

• Korean Journal of Environment and Ecology
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• v.9 no.1
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• pp.56-61
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• 1995

To examine the effects of gibberelin treatment on seed germination of six wild herbaceous species -Dicentra spectabilis, Aster pekinensis, Agastache rugosa, Elsoholtzia ciliata, Boehmeria tricuspis and Pulsatillia cerrma var. chaerophyiloides - in Kangwon-do, pretreated seeds of five species were sowed on plastic pots with mixed soil(sand：peat moss：perlite： vermiculite, 1：1：1：1, v/v) in 23,April 1995. Seeds were soaked in 25,50 ppm gibberelin solution and ground water during 24 hours. Pretreated seeds of Pulsatiilia cerrma var. chaerophylloides were sowed on nursery soil in 1, June 1995. Seed pretreatments in 31, May 1995 were same method. Seed germination rate was measured and compared among species and treatments. Seed germination rate of Dicentra spectabilis, Agastache rugosa and Elsoholtzia ciliata was differed higly significant among pretreatments ,but those of that of Aster pekinensis, Boehmeria tricuspis and Pulsatillia cerrma var chaerophylloides did not significant at 5% level. Gibberelin pretreatment raised seed germination rate of Dicentra spectabilis and Agastaohe rugosa and proper concentration of GA$_3$ was 25ppm and seed germination rate of Elsoholtzia ciliata was highest at GA$_3$ 50ppm plot. The utilization of wild herbaceous species were discussed.

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.1
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• pp.43-48
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• 2010

This study was designed to evaluate whether the addition of sediment improvements with natural materials to water tanks influenced the hematologic properties and muscle glycogen content of the blood cockle, Tegillarca granosa under laboratory conditions. Following a 10-d matured period of sediment improvements, blood cockles were subjected to 4 groups (0, 100, 300, $800\;g/m^2$) of sediment improvements for 14-d Only one individual died at $800g/m^2$ after 48-h Whole blood, hematocrit value and hemoglobin concentration of blood cockle were changed little by sediment improvements, however, both glucose and glycogen were increased more compared with those of the control groups.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.E2
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• pp.74-81
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• 2007

Feasibility test for a biofilter was performed to treat VOCs. The applied loading rate to the biofilter was calculated between 60 to $3,700\;kg\;COD/m^3$. Trimethyl-pentene and trimethyl-hexene were the two most dominant compounds and they occupy about 85 percent. During the acclimation period, it is desirable for a biofilter to receive relatively lower VOCs concentration and flow rate, until it can adjust to new substrate and operational environment. Temperature at various points inside the biofilter reactor was observed with more than 23 temperature sensors. With steam heating, temperatures of the top sections of the media were greater than those of bottom sections. Without steam heating, intermediate stages generally had higher temperature measurement than those of bottom and top stages. Because the pH values for different biofilter materials vary significantly, measurement of the pH for the mixture of different combinations of biofilter materials is necessary. Based on the types and brands of media, the measured pH ranged from 5.38 to 9.10. The range of measured pH of different mixtures with perlite, compost, saw dust, peat moss, limestone, vermiculite was 7.05 to 8.62.

• Korean Journal of Medicinal Crop Science
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• v.10 no.4
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• pp.249-252
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• 2002

Several plant growth regulators were examined for on their effect on the in vitro propagation of Kalopanax pictus$(T_{HUNB}.)$ $(N_{AKAI}.)$ on WPM medium. Among the cytokinins tested, BA at $13.32\;{\mu}M$ appeared to be the most effective for multiple axillary shoot formation. Although the addition of $2.89\;{\mu}M\;GA_3$ promoted stem elongation, it produced morphologically abnormal leaves and stems. For rooting of the shoots, $4.9\;{\mu}M$ IBA seemed to be more effective than $2.69\;{\mu}M$ NAA. When the regenerated plants were transferred on artificial mixture containing vermiculite and peat-moss (1 : 1, v/v), 81% of them survived and grew normally.

• Ocean and Polar Research
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• v.26 no.3
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• pp.531-537
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• 2004

In this study we discuss some problems that emerged from paleolimnological and paleontological investigations of terrestrial Holocene ecosystems on King George Island (South Shetland Islands) conducted by an Argentine-Polish research group. Biological and geochemical markers commonly used in standard analytical procedures are considered insufficient in tracing overlapping records of past environmental changes preserved in peat banks, lake sediments and ornithogenic remnants. Records that might be explained by predictable natural events (related to glacio-isostatic uplift of land), roughly predictable events (ecological succession), or unpredictable events (volcanic eruptions or accidental destruction of aquatic moss) may overlap or interfinger one with another providing that signals of regional and/or global climatic changes, are hardly identifiable. A more sophisticated and more selective methods are recommended to do discrimination between records of local and regional/golbal processes in studies on Holocene climatic history of the South Shetland Islands.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2002.04a
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• pp.54-56
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• 2002

고추는 국내 재배면적이 노지재배가 75,000 ha 정도로서 해마다 조금씩 감소하고 있고, 시설재배가 5,000 ha 정도로서 해마다 증가하고 있다. 따라서 약 80,000 ha 의 재배 면적을 매년 심어야 하기 때문에 원예작물 가운데 가장 많은 모종을 필요로 한다. 이를 뒷받침하기 위하여 공정육묘에서 차지하는 비율이 60%를 넘고 있다. 고추종자는 발아적온이 28-3$0^{\circ}C$ 정도로 알려져 있으며 발아소요일수가 2-3일 정도로서 발아적산온도는 1,300~1,450$^{\circ}$ 정도로 알려져 있다. (중략)