• Title/Summary/Keyword: Mixed Extracts

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Weed Control Efficacy of the Residues and its Aqueous Extract of Sorghum Shoots (수수 지상부의 부산물과 추출물의 제초활성)

  • Park, Su Hyuk;Won, Ok Jae;Le, Thi Hien;Eom, Min Yong;Hwang, Ki Seon;Hwang, Jae-Bok;Park, Kee Woong
    • Weed & Turfgrass Science
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    • v.4 no.3
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    • pp.243-248
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    • 2015
  • This study was conducted to investigate the ability for weed control of the shoot extract of sorghum (Sorghum bicolor (L.) Moench) and developing a sustainable weed management in organic farming. When the dried shoot powder was mixed with soil and treated with 2 cm above the soil surface, the germination for Echinochloa crus-galli, Digitaria cilialis, Abutilon theophrasti and Amaranthus retroflexus was inhibited maxium 40%. The growth inhibition of germinated seedlings in the A. theophrasti and A. retroflexus was maxium 30% while it was less than 30% in the E. crus-galli and D. cilialis. Shoot extracts at rates above $25mg\;ml^{-1}$ was effective to inhibit germination of D. cilialis, A. theophrasti and A. retroflexus. The shoot extract concentration required for 50% of germination inhibition was $60mg\;ml^{-1}$ in the E. crus-galli, while it was less than $10mg\;ml^{-1}$ in the D. cilialis, A. theophrasti and A. retroflexus. For the foliar application, 11 adjuvants were tested at 0.5% and DOS70, TM15 and TDE7 were most effective adjuvants for the shoot extracts. DOS70 was most effective and provided up to 60% of weed control efficacy for the tested four weed species. Though herbicidal efficacy of sorghum shoot was not enough to give a proper weed control, it can be expected that long term use of sorghum shoots can provide gradual decrease in weed seeds and weed density.

Antioxidant and α-Glucosidase Inhibitory Activities of the Extracts of Aster koraiensis Leaves (국내산 벌개미취 잎 추출물의 α-glucosidase 억제능 및 항산화 활성 평가)

  • Lee, Tae Gu;Hyun, Soo Wang;Lee, Ik Soo;Park, Bong Kyun;Kim, Jin Sook;Kim, Chan Sik
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.5
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    • pp.382-390
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    • 2018
  • Background: The plant Aster koraiensis has long been used as an ingredient in folk medicine. It has been reported that Aster koraiensis extract (AKE) prevents the progression of diabetes-induced retinopathy and nephropathy. However, although these beneficial effects of AKE on diabetes complications have been identified, the antidiabetic effects of AKE have not yet been completely investigated and quantified. In the present study, the glucose-lowering and antioxidant effects of aqueous and ethanolic AKEs were evaluated. Methods and Results: The glucose-lowering effects of aqueous and ethanolic (30%-, 50%-, and 80%-ethanol) AKEs were investigated via ${\alpha}$-glucosidase inhibitory assays. The mode of inhibition by AKEs on ${\alpha}$-glucosidase was identified through kinetic analysis. The total antioxidant capacity of each of the 4 AKEs was evaluated by assessing their conversion rate of $Cu^{2+}$ to $Cu^+$. The content of chlorogenic acid and 3,5-di-O-caffeoylquinic acid, the bioactive compounds in AKE, in each extract were analyzed by high performance liquid chromatography (HPLC). The AKEs showed potent ${\alpha}$-glucosidase inhibitory activity with mixed inhibition mode, and significant antioxidant capacity. Conclusions: These results of this study suggested that the AKEs tested had ${\alpha}$-glucosidase inhibitory and antioxidant effects. Among the extracts, the 80% ethanol extract showed the most significant ${\alpha}$-glucosidase inhibitory activity, with a half maximal inhibitory concentration ($IC_{50}$ value) of $1.65{\pm}0.36mg/m{\ell}$ and a half maximal effective concentration ($EC_{50}$ value) for its antioxidant activity of $0.42{\pm}0.10mg/m{\ell}$. It can therefore be used as a source of therapeutic agents to treat diabetes patients.

Low-temperature Rendering Technology Applied to Extract Black Beans, Peony and Green Tea for Scalp Repair (저온용출법을 이용한 검은콩, 작약, 녹차 추출물의 두피 개선 효과)

  • Min, Dae-Jin;Park, Nok-Hyun;Hwang, Jung-Sun;Moon, Sung-Ho;Lee, Ki-Hyun;Lee, John-Hwan;Ahn, Soo-Mi;Kim, Han-Kon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.35 no.1
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    • pp.41-46
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    • 2009
  • Problems with scalp lead to hair loss, dandruff, itchiness, and other illness. No clear causes of these problems have been found and it is difficult to treat them. Therefore, the best way to prevent and treat any problems with scalp is to maintain physiological homeostasis of scalp to keep it healthy. Recently, many scalp and hair product brands have attempted to use medicinal herbs which have been extracted in hot water and mixed with other ingredients due to mass-production and standardization issues. However, many nutrients and active substances are destroyed by hot-water extraction. Therefore, this study has applied low-temperature rendering to minimize destruction of substances to extract black beans, peony, and green tea that are known to improve conditions of scalp. Then, their contribution to the improvement of scalp health was assessed. In result, it was found that low-temperature rendering retains over two times greater anti-oxidizing strengths than hot-water extraction and that the extracts from low-temperature rendering effectively strengthen follicles and hair, moisturize scalp, and prevent itchiness. Therefore, low-temperature rendered black beans, peony, and green tea extracts can be used to make effective scalp treatments.

Effect of a Mixture of Extracts from Residues of Onion Left after Onion Harvesting and Purslane (Portulaca oleracea) on Productivity and Quality Characteristics of Organic Onions (양파 수확 후 잔재물과 쇠비름 추출물이 유기농 양파의 수확량 및 품질 특성에 미치는 영향)

  • Kim, Tae-Won;Jeon, Byeong-Gyun;Lee, Sung-Ho
    • Journal of Life Science
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    • v.27 no.12
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    • pp.1430-1436
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    • 2017
  • This study assessed the effects of treatment with sap extract from onion residues postharvest and purslane on the quality and quantity of organic onions. At the bending stage, onions treated with the sap extract showed vigorous growth, with higher plant heights, more leaves, and longer sheath lengths than untreated onions. The onion yield was significantly increased when the plant was treated with extracted sap as compared with that of untreated plants (p<0.05). The bulb weight distribution of onions in the mixed onion and purslane treatment was also significantly increased (~300 g) as compared with that of the other treatment (p<0.05). Except for CaO and S, the mineral content of the onions produced from plants treated with the onion and purslane extract mixture was higher than those of onions in the other treatment. The hardness of onions produced from plants treated with the onion and purslane extract was significantly increased (8% and 20%, respectively) as compared with that of onions produced from plants treated with the onion extract only or no treatment (p<0.05). However, there was no significant difference in the sugar contents of the onions produced from extract-treated and nonextract-treated plants. Postharvest, the content of inorganic components (phosphate, calcium, sulfuric acid, and manganese) was higher in soil treated with the onion extract than in soil treated with the onion and purslane extract and non-treated soil. It can be concluded that residues left after onion harvests and purslane extract can be used as natural and environmentally friendly materials for the cultivation of organic onions.

Optimization of Extraction Conditions for Mixing Beverage Development of Black Garlic and Gaeddongssuk by Response Surface Methodology (반응표면분석을 이용한 흑마늘과 개똥쑥 혼합음료 개발을 위한 추출조건의 최적화)

  • Kang, Jae-Ran;Lee, Soo-Jung;Hwang, Cho-Rong;Shin, Jung-Hye;Kang, Min-Jung;Sung, Nak-Ju
    • Journal of agriculture & life science
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    • v.46 no.2
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    • pp.139-149
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    • 2012
  • Black garlic and Gaeddongssuk (Artemisia annua L.) were extracted using central composite design by response surface methodology (RSM) in 11 conditions (A~K) with $110{\sim}130^{\circ}C$ and 2~4 hr, and then each extracts were monitored for the total phenol, flavonoid contents and antioxidant activities. The optimum extraction conditions for black garlic and Gaeddongssuk are selected 3 conditions ($130^{\circ}C$, 4 hr; $120^{\circ}C$, 3 hr; $130^{\circ}C$, 3 hr) and 1 condition ($120^{\circ}C$, 3 hr), respectively. They were mixed according to the following ratio; 1:0.5, 1:1, 1:1.5 and 1:2 (black garlic: Gaeddongssuk extract, w/w), and then these composites were tested to the total phenol, flavonoid contents and antioxidant activities. Antioxidant activities of black garlic and Gaeddongssuk extracts by $120^{\circ}C$, 3 hr condition were higher. And the optimal mixture ratio of black garlic and Gaeddongssuk was 1:1.5 (w/w).

Effect of Ethanolic Extracts Mixed with Grains and Fallopia multiflora on Melanogenesis (곡물과 적하수오를 혼합한 에탄올 추출물이 멜라닌 합성에 미치는 영향)

  • Lee, Eunbeen;Kim, Moon-Moo
    • Journal of Life Science
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    • v.29 no.4
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    • pp.461-469
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    • 2019
  • The aim of this study was to investigate the effect of a mixture of ethanol extracts of Black oryzasativa, Sesamum indicum, Oryza sativa, Rhynchosia Nulubilis, and Polygoni multiflori radix (MIXEE) on melanogenesis to develop a natural product for black hair growth. An accumulation of hydrogen peroxide ($H_2O_2$) in hair follicles, which reduces melanin synthesis, is responsible for hair graying. In the present study, MIXEE showed scavenging activity against DPPH radicals and reducing power. In addition, it reduced the cellular $H_2O_2$ level, indicating that it could inhibit oxidation and promote melanin synthesis, which was decreased by $H_2O_2$. On the other hand, it did not affect tyrosinase activity in vitro but promoted the turnover of L-DOPA into melanin. MIXEE promoted melanin synthesis at the cellular level in B16F1 cells. Furthermore, MIXEE increased the expression levels of superoxide dismutase 2 (SOD2) and SOD3 in western blot analysis. In addition, MIXEE increased the expression levels of tyrosinase-related protein (TRP)-2, which promoted melanin synthesis from L-DOPA. The results suggested that MIXEE could promote melanogenesis. Therefore, MIXEE may have potential as a natural product for promotion of melanin production and reversal of gray hair to black hair.

Evaluation of Cytotoxicity by Extracting Ginseng, Processed Aconitum, Ginger, and Licorice (인삼, 숙부자, 생강, 감초의 배합에 의한 세포독성 평가)

  • Kim, D.H.;Kim, Y.B.;Koo, H.J.;Baek, H.J.;Lee, S.B.;Chang, K.J.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.23 no.1
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    • pp.31-36
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    • 2021
  • Cytotoxicity was evaluated in A549 lung cancer cells and RAW264.7 macrophage cells with processed aconitum, ginseng, ginger and licorice extracts. The first experiment began to affect toxicity from 100 ㎍/ml concentrations in extracts mixed with processed aconitum and ginseng. Cytotoxicity began at 1000 ㎍/ml concentrations in the second experimental extract with additional ginger, both in the first and second groups affected greater cytotoxicity in lung cancer cells than in macrophage cells, and in the third experimental extract with additional ginger and licorice. In conclusion, when using aconitum, ginseng, ginger, and licorice work in combination, which resulted in less impact on macrophage cells toxicity and more cytotoxicity in certain lung cancer cells.

Optimization of mixing ratio of Polygala tenuifolia, Angelica dahurica and Elsholtzia splendens extracts for cosmetic material development (화장품 소재 개발을 위한 원지 (Polygala tenuifolia), 백지(Angelica dahurica) 및 꽃향유 (Elsholtzia splendens) 추출물의 혼합 비율 최적화)

  • Jung Seo A;Song, Ga Hyeon;Su In Park;Jung, Youn Ok
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.6
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    • pp.993-1000
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    • 2023
  • Recently, enviromentally friendly natural substances derived from plants have been attracting attention as cosmetic materials, and research on various physiological activities of natural substances is being actively conducted. This study investigated the antioxidant, anti-inflammatory, moisturizing, and antibacterial effects of three types of extracts of mixtures containing different mixing ratios, Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens, known to have various physiological activities. The mixing ratio is 7 conditions (M1, 1:1:1; M2, 0.5:1.5:1; M3, 1.5:0.5:1; M4, 0.1:0.95:0.95; M5, 0.5:0.5:2; M6, 0.95 :1.95:0.1; M7, 1.45:0.1:1.45), and the optimal mixing ratio was confirmed for use as a cosmetic material. DPPH and ABTS radical scavenging activities showed scavenging abilities of 75.37% and 99.19%, respectively, at 1,000 ㎍/mL of M6. At a concentration of 200 ㎍/mL of M6, it showed 50% of nitric oxide production inhibition compared to the lipopolysaccharide-treated that induced an inflammatory response. It was confirmed that M3 and M6 produced hyaluronic acid 1.47 and 1.49 times higher than the control at a concentration of 50 ㎍/mL, respectively. Through the disc diffiusion test, the clear zone was 9.75 mm at 8 ㎍/mL of M6, confirming the inhibition of growth of staplylococcus aureus strain. Based on the above results, it is believed that the mixed extract of Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens can be used as a functional natural material for cosmetics.

Anti-inflammatory and anti-bacterial effects of a mixture of Syzygium aromaticum and Coptis japonica (정향과 황련 혼합물의 항균 및 항염증 활성 연구)

  • Eunhong Lee;Eun-mi Jung;Hyun-Ji Kwon;Jihye Lee;BongHyun Woo;Sungmin Park;Jinhan Park;Ji Wook Jung
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.6
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    • pp.1547-1556
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    • 2023
  • This study was conducted to check the anti-bacterial ability against Malassezia furfur by mixing Szygium aromaticum and Coptis japonica extracts and to evaluate the antioxidant and anti-inflammatory ability by creating an optimal mixture. Szygium aromaticum and Coptis japonica were extracted with 70% ethanol, 100% methanol, and water to evaluate the antibacterial ability, and it was confirmed that 100% methanol extract of Szygium aromaticum and Coptis japonica water extract had the highest anti-bacterial ability. In addition, when the two extracts were mixed and the anti-bacterial ability was evaluated by ratio, the ratio of 9:1 showed the best activity, and it was confirmed that the antioxidant activity of the mixture was excellent. In Raw 264.7 cells, LPS was used to induce inflammatory responses and confirmed anti-inflammatory activity at 1, 10, 50, and 100 ㎍/mL that did not affect survival, and it was confirmed that NO-production inhibition and IL-6 expression inhibition and COX2 and iNOS protein expression inhibition activity were excellent at 10, 50, and 100 ㎍/mL. Through this study, it is thought that the mixture of Szygium aromaticum100% methanol extract and Coptis japonica water extract can be used as a natural ingredient in functional cosmetics because of its excellent antibacterial and anti-inflammatory effects.

Effect of Dietary Supplementation of Extracts of Mushroom Mycelium on Survival and Growth of juvenile Flounder, Paralichthys olivacceus (버섯균사체 배양액 첨가사료가 넙치 치어의 생존 및 성장에 미치는 영향)

  • Kim, Min-Ju;Kim, Man-Chul;Kim, Taeg;Kim, Ki-Young;Song, Chun-Bok;Jeon, Yu-Jin;Heo, Moon-Soo
    • Journal of Aquaculture
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    • v.19 no.4
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    • pp.231-235
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    • 2006
  • The effects of mycelium cultural extract supplemented diet, hematology and disease resistance against Vibrio anguillarum in juvenile flounder, Paralichthys olivaceus were evaluated. Fish were fed the Phellinus linteus with Coriolus militaris versicolor mixed mycelium cultural extract supplemented diet, Phellinus linteus mycelium cultural extract supplemented diet and Coriolus militaris tmycelium cultural extract supplemented diet a commercial diet for 12 week. The body weight and length gain from the fish fed on daily the phellinus with coriolus versicolor mixed mycelium cultural extract supplemented diet, phellinus linteus mycelium cultural extract supplemented diet and Coriolus versicolor mycelium cultural extract supplemented diet of each mycelium cultural extract were higher than the control and the Glutamic oxalacetic transaminase (GPT) were of lower than the control. The relative precent survival rate (RPS) after an artificial challenge with $7{\times}10^5\;CFU$ of Vibrio anguillarum per fish was higher than the control.