Optimization of Extraction Conditions for Mixing Beverage Development of Black Garlic and Gaeddongssuk by Response Surface Methodology

반응표면분석을 이용한 흑마늘과 개똥쑥 혼합음료 개발을 위한 추출조건의 최적화

Black garlic and Gaeddongssuk (Artemisia annua L.) were extracted using central composite design by response surface methodology (RSM) in 11 conditions (A~K) with $110{\sim}130^{\circ}C$ and 2~4 hr, and then each extracts were monitored for the total phenol, flavonoid contents and antioxidant activities. The optimum extraction conditions for black garlic and Gaeddongssuk are selected 3 conditions ($130^{\circ}C$, 4 hr; $120^{\circ}C$, 3 hr; $130^{\circ}C$, 3 hr) and 1 condition ($120^{\circ}C$, 3 hr), respectively. They were mixed according to the following ratio; 1:0.5, 1:1, 1:1.5 and 1:2 (black garlic: Gaeddongssuk extract, w/w), and then these composites were tested to the total phenol, flavonoid contents and antioxidant activities. Antioxidant activities of black garlic and Gaeddongssuk extracts by $120^{\circ}C$, 3 hr condition were higher. And the optimal mixture ratio of black garlic and Gaeddongssuk was 1:1.5 (w/w).

흑마늘과 개똥쑥을 반응표면분석법에 따른 중심합성계획에 의하여 $110{\sim}130^{\circ}C$와 2~4 시간 범위의 11가지 조건(A~K)에서 각각 열수추출하여 총 페놀, 플라보노이드 함량 및 항산화 활성이 최대화되는 추출조건을 모니터링 하였다. 항산화 활성의 정도에 따라 흑마늘 추출조건 3종($130^{\circ}C$, 4 hr; $120^{\circ}C$, 3 hr; $130^{\circ}C$, 3 hr)과 개똥쑥의 최적 추출조건 1종($120^{\circ}C$, 3 hr)을 선정하였다. 흑마늘 추출물에 대한 개똥쑥 추출물의 혼합비율을 1:0.5, 1:1, 1:1.5 및 1:2(w/w)로 조절하여 총 페놀, 플라보노이드 함량 및 항산화 활성을 측정한 결과, $120^{\circ}C$, 3시간에서 추출된 흑마늘 추출물과 개똥쑥 추출물의 혼합시 활성이 우수하였으며 최적의 혼합비율은 흑마늘과 개똥쑥 추출물에서 1:1.5(w/w)인 것으로 판단된다.