• Journal of the Korean Society of Safety
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• v.30 no.1
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• pp.94-103
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• 2015

This study analyzes the influence factors on elderly pedestrian accident. Elderly people are easy to be badly injured by car accidents compared to younger people. Therefore, various plans and measures are required to protect elderly pedestrian from accidents. However, pedestrian accidents studies only focused on microscopic factors such as attribute of driver, pedestrian, road design. In order to prevent pedestrian accident and reduce the severity of the accident, not only microscopic factors but macroscopic variables such as urban planning and facility should be considered. In this regard, this study develops an ordered probit model introduced the characteristics of urban facility which were not considered in the previous studies. The result shows that there is higher level of accident severity in such areas as large commercial area, well-developed area with transportation infrastructure service and non-pedestrian safety zone. Thus, various and appropriate countermeasures should be prepared in order that pedestrian accident can be prevented in the areas mentioned above. In addition to the aforementioned variables, it is revealed that other variables including vehicle speed, gender and age of pedestrian, weather condition, type of vehicle, etc. partly affect the severity of pedestrian accident.

• Applied Microscopy
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• v.20 no.2
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• pp.57-70
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• 1990

Characterization and electron microscopic visualization of the plasmid and the gene expression of Escherichia coli were carried out. Transcriptional units of active structural genes were observed after lysis of Escherichia coli cells. The ribosomes attached to the E. coli genome on mRNA molecule as polyribosomes. From this gradient of polyribosome length, we estimated location of mRNA synthesis initiation site. In this experiment, a granule is ofen present which may correspond to a RNA polymerase at the promoter site. pOX1, pOX7, pOX7A, $pOX7{\Delta}1$, pSTP36, pSTP21, pBR322, and pJH12 were visualized by way of electron microscope, and their estimated sizes were determined to be $5.70{\pm}0.08{\mu}m,\;2.15{\pm}0.10{\mu}m,\;2.14{\pm}0.12{\mu}m,\;7.39{\pm}0.08{\mu}m,\;4.03{\pm}0.04{\mu}m,\;1.50{\pm}0.03{\mu}m\;and\;1.25{\pm}0.09{\mu}m$ respectively. One micrometer of measured length corresponded to about 3.0 Kb. Mica-press adsorption method that allows selectivs visualization of the plasmid DNA released in situ from the bacterial cell is rapid and useful for visualization of plasmids. The released plasmid DNA was adsorbed preferently on mica in a divalent cation-free solution. Miller chromatin-spreading method was useful to observe the plasmid and transcripts. BAC method and cytochrome C monolayer were useful to observe the plasmid DNA. Our ability to visualize ultrastructural aspects of the expression of E. coli has given us a unique tool with which to study the regulation the level of an individual gene.

• Journal of Korean Society of Transportation
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• v.22 no.2 s.73
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• pp.95-102
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• 2004

The COSMOS is an adaptive traffic control systems that can adjust signal timing parameters in response to various traffic conditions. To evaluate the performance of the COSMOS systems, the field study is only practical option because any evaluation tools are not available. To overcome this limitation, a newly integrated interfacing simulator between a microscopic simulation program and COSMOS was developed. In this paper, a detector module and a signal timing module as well as general feature of the simulator were described. A validation test was performed to verify the accuracy of the data flow within the simulator. It was shown that the accuracy level of information from the simulator was high enough for real application. Several practical comments on further studies were also included to enhance the functional specifications of the simulator.

• Spatial Information Research
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• v.23 no.4
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• pp.79-89
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• 2015

This study aims to design and implement a traffic model that can simulate the traffic behavior on the microscopic level by using the GIS. In the design of the model, the vehicle in the simulation environment recognizes the GIS road centerline data as road network data reflecting number of lanes, speed limit and so on. In addition, the behavior model was designed by dividing functions into the environmental perception model, time headway distribution model, car following model, and lane changing model. The implemented model was applied to Jahamun-road of Jongno-gu district to verify the accuracy of the model. As a result, the simulation results on the Jahamun-road had no great error compared with the actual observation data. In the aspect of usability of model, it is judged that this model will be able to effectively contribute to analysis of amount of carbon emission by traffic, evaluation of traffic flow, plans for location of urban infrastructure and so on.

• Korean Journal of Veterinary Research
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• v.42 no.2
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• pp.153-162
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• 2002

For the induction of arthropathy, 5% hydrogen peroxide($H_2O_2$) was injected for 5 weeks into the intraarticular space of the New Zealand white rabbits to damage articular cartilage. Alginic acid of low molecular weight (2%) made from macromolecular alginate treated with enzyme was administered into articular space at the dose of 5 mg/kg twice a week for 3 and 6 weeks using 1 ml syringe and 26 G needle. Saline was injected for the control. Tissues surrounding the articulation were obtained for the measurements of superoxide dismutase(SOD) activity as a major antioxidant enzyme and malondialdehyde (MDA) as a lipid peroxidation level. Histopathologic examination on the surface of articular cartilage was carried out. Data showed that injection of hydrogen peroxide for 5 weeks had led to the induction of free radical damage and of articular cartilage change as confirmed by microscopic observation. The application of hydrogen peroxide caused a gradual increase in the SODs and MDA. These patterns were similar after 3 and 6 weeks of alginate treatment. Furthermore, microscopic examinations revealed that hydrogen peroxide caused flaking, fibrillation, fissuring, denudation, and hypocellularity in the articular surfaces. In conclusion, lipid peroxidation was demonstrated in the articular cartilage by the administration of hydrogen peroxide in the rabbit model. This lipid peroxidation could be caused by oxygen free radicals. The histologic and enzymatic correlations on lipid peroxidation in the articulation have provided a better understanding of arthropathy. It is possible to take advantage of these findings to evaluate effective alginate dosage more efficiently.

• Journal of Korean Society on Water Environment
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• v.20 no.5
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• pp.506-511
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• 2004

Maintaining dissolved oxygen (DO) at sufficiently low concentration in the aeration tank at a wastewater treatment plant (WWTP) is essential for reduction of the costs of operation and maintenance. On the other hand, the low DO level may result in adverse effect on the integrity of the activated sludge, A typical and disastrous outcome frequently experienced is the outgrowth of filamentous microorganisms, which is called as filamentous bulking, In addition to the traditional methods such as sludge settleability and microscopic observation of the culture, molecular techniques including polymerase chain reaction (PCR) amplification followed by 16S rRNA sequencing were applied to identify filamentous bacteria present in bulking sludge under a condition of low DO concentration, Two morphologically distinct groups, presumably consisting of Sphaerofilus nafans, and Eikelboom Type 1701 or Type 1851, were identified through microscopic observation. They were further confirmed by subsequent 16S rRNA sequencing. Dominant filamentous bacteria identified by the molecular techniques were consisted of three major groups. Sequences of partial 16S rRNA cloned showed that the filamentous bulking organisms were closely related to Eikelboom Type 021N and Eikelboom Type 1701, and Sphaerotilus natans, respectively. Molecular methods were found to possess a strong potential of direct examination of the microbial community of an activated sludge system.

• Economic and Environmental Geology
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• v.30 no.4
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• pp.353-362
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• 1997

P wave velocities of core samples from the Pocheon granite were measured before and after applications of cyclic loading. Then. distribution of the pre-existing microcracks and microcracks developed due to the cyclic loading was investigated by analyzing P wave velocity anisotropies and microscopic observations from thin sections. Anisotropy constants were calculated with three different ways: (1) $C_A$ between the maximum and the minimum velocities, (2) $C_AI$ between velocities measured along the axial direction and the average of six velocities measured in the planes perpendicular to the loading axis (rift plane) and (3) $C_AII$ between the maximum and the minimum velocities measured in the plane perpendicular to the loading axis. Among anisotropy constants. $C_AI$ was the most effective anisotropy constant to identify the rift plane whose orientation is parallel to the pre-existing microcracks as well as the distribution of stress induced microcracks. $C_AI$ decreased after cyclic loading and the relationship between $C_AI$ and number of cycles shows comparatively coherent negative trends. indicating that stress induced microcracks are aligned perpendicular to the orientation of pre-existing microcracks and that the amounts are proportional to the number of loading cycles. The difference of anisotropy constants before and after cyclic loading was effective in delineating the level of cracks and we called it Induced Crack Index. Velocity measurements and microscopic observations show that anisotropy was caused mainly due to microcracks aligned to a particular direction.

• Bulletin of the Korean Chemical Society
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• v.30 no.1
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• pp.214-218
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• 2009

Second-order rate constants ($k_N$) have been measured for nucleophilic substitution reactions of S-4-nitrophenyl thiobenzoate with a series of alicyclic secondary amines in $H_2O$ containing 20 mol % DMSO at 25.0 ${\pm}$ 0.1 ${^{\circ}C}$. The Br$\phi$nsted-type plot exhibits a downward curvature, i.e., $\beta_{nuc}$ decreases from 0.94 to 0.34 as the amine basicity increases. The reactions in the aqueous DMSO have also been suggested to proceed through a zwitterionic tetrahedral intermediate (T${\pm}$) with change in the RDS on the basis of the curved Br$\phi$nsted-type plot. The reactions in the aqueous DMSO exhibit larger $k_N$ values than those in the aqueous EtOH. The macroscopic rate constants ($k_N$) for the reactions in the two solvent systems have been dissected into the microscopic rate constants ($k_1\;and\;k_2/k_{-1}$ ratio) to investigate effect of medium on reactivity in the microscopic level. It has been found that the $k_2/k_{-1}$ ratios are similar for the reactions in the two solvent systems, while $k_1$ values are larger for the reactions in 20 mol % DMSO than for those in 44 wt % EtOH, indicating that the larger $k_1$ is mainly responsible for the larger $k_N$. It has been suggested that the transition state is more stabilized in 20 mol % DMSO through mutual polarizability interaction than in 44 wt % EtOH through H-bonding interaction.

• Journal of Ocean Engineering and Technology
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• v.26 no.5
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• pp.73-80
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• 2012

It is well known that a considerable amount of scatter is shown in experimental results relating to fatigue crack growth even under identical and constant amplitude cyclic loading conditions. Moreover, flux cored arc welding (FCAW) is a common method used to join thick plates such as the structural members of large scale offshore structures and very large container ships. The objective of this study was to investigate the macro- and microscopic observations of the fatigue crack growth (FCG) behavior of the FCAWed API 2W Gr. 50 steel joints typically applied for offshore structures. In order to clearly understand the randomness of the fatigue crack growth behavior in the materials of three different zones, the weld metal (WM), heat affected zone (HAZ), and base metal (BM), experimental fatigue crack growth tests for each of five specimens were performed on ASTM standard compact tension (CT) specimens under constant amplitude cyclic loading. Special focus was placed on the fatigued fracture surfaces. As a result, a different behavior was observed at the macro-level, depending on the type of material property: BM, HAZ, or WM. The variability in the fatigue crack growth rate for WM was higher than that of BM and HAZ.

• Korean Security Journal
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• no.16
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• pp.283-304
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• 2008

The development of IT technology help citizens demand information and public services through internet that they want, and the changes also help the government converge public opinions and become important measures for citizens to participate in deciding governmental policies. So, this thesis makes an overture of a possibility for utilizing PCRM, a way for citizens to participate in Police Administration, by utilizing IT technologies in which everybody can participate without difficulty. The utilization ways are, on a large scale, classified with a macroscopic aspect and microscopic aspect. One of the macroscopic aspects is, first, the consideration of access to services and convenience in utilization. Second, services should have a both-sidedproperty. Ultimately, citizens'participation should have a positive mutual participation level. One of the microscopic aspects is, first, that the police organizations have to establish a strategy to analyze and fractionate customers. Second, the police organizations have to make an effort to develop policy services and P.R strategies. Third, police organizations have to make an effort to manage customers. Fourth, police organizations have to execute customer management in general. So, all of the police organizations have to maintain unity, to some extent, about citizen participation plazas. In addition, at the same time, consciousness aspects have to be accompanied for the reasonable utilization of PCRM. Citizens have to cultivate citizen consciousness, ownership consciousness for society, and positive participation consciousness to solve social problems.