• Tunnel and Underground Space
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• v.8 no.4
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• pp.307-320
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• 1998

Tunnel lining is the final support of a tunnel and reflects the results of the interaction between ground and support system. Recently it is very difficult to support and manage the tunnel because the cracks on tunnel lining cause many problems in supporting and managing tunnels. Therefore the analysis of the cracks is quite strongly required. In this study, mechanical behaviour of a tunnel lining was examined by model tests and by numerical analyses. Especially the model test was examined for double linings including shotcrete and concrete lining. The model tests were carried out under various conditions taking different loading shapes, horizontal stresses, thicknesses of linings and double lining, vault opening behind the concrete lining and rock-like medium surrounding the lining. Due to horizontal stress, compressive stress prevailed on the lining. Thus the bearing capacity of the lining increased. The existence of a vault opening behind the concrete lining reduced the bearing capacity by the similar amount of reduction of concrete lining thickness. Rock-like medium cast around the side wall of the lining restrained the deflection of the lining, and the bearing capacity for cracking and failure increased vary much. In numerical analyses a algorithm which can analysis the double lining by introduction of interface element was developed. And the results of the numerical analyses were compared with the results of the model tests.

• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.34-39
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• 2003

Corm apical meristem cultures of thirteen glasshouse freesia cultivars were tested to investigate the possibility of micropropagation using MS basal medium supplemented with 2,4-D, NAA(0.1, 0.5, 1.0mg/L, respectively) and BA (0.5∼2.0mg/L). The majority of the tested cultivars could be induced callus and shoot buds in all culture condition. The combinations of NAA and BA appeared superior to that of 2,4-D and BA depending on cultivars for callus induction and shoot formation. Among the cultivars, 'Golden Yellow' showed the highest regeneration capacity on MS media with 0.5mg/L NAA and 1.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoot from calli were obtained through successive subculture on MS medium supplimented with 0.5mg/L BA. In that condition, more than 60 ％ shoot regeneration and average of 25.1 shoots per explant was achieved. Transformed shoots on half-strength MS medium without plant growth regulators rooted easily.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.365-373
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• 2006

This study was conducted to develop the efficient propagation method of fern Phyllitis scolopendrium using In vitro culture. The influence of the origin of the donor explant sources (rhizome, stipe, three parts of blade) and the homogenization of explants was investigated. Rhizome and stipe explants showed the organogenic capacity among the five explant sources and plant regeneration was promoted by homogenization of culture material. Optimum condition for vigorous and excellent growth of multiple shoots was the half-strength MS medium with 1% sucrose concentration. Generally, addition of $NaH_2PO_4$ to media enhanced shoot multiplication. The highest rate of shoot proliferation was observed on the media containing $5{\mu}M$ NAA. Also, combination of activated charcoal $(0.1{\sim}0.2%)$ and growth regulators to growth medium prevented the formation of multiple bud primordia, 'nodule'-like bud clusters and improved the normal morphogenesis of sporopytes in P. scolopendrium.

• Earthquakes and Structures
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• v.11 no.5
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• pp.741-777
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• 2016

A simplified approach of assessing torsionally balanced (TB) and torsionally unbalanced (TU) low-medium rise buildings of up to 30 m in height is presented in this paper for regions of low-to-moderate seismicity. The Generalised Force Method of Analysis for TB buildings which is illustrated in the early part of the paper involves calculation of the deflection profile of the building in a 2D analysis in order that a capacity diagram can be constructed to intercept with the acceleration-displacement response spectrum diagram representing seismic actions. This approach of calculation on the planar model of a building which involves applying lateral forces to the building (waiving away the need of a dynamic analysis and yet obtaining similar results) has been adapted for determining the deflection behaviour of a TU building in the later part of the paper. Another key original contribution to knowledge is taking into account the strong dependence of the torsional response behaviour of the building on the periodic properties of the applied excitations in relation to the natural periods of vibration of the building. Many of the trends presented are not reflected in provisions of major codes of practices for the seismic design of buildings. The deflection behaviour of the building in response to displacement controlled (DC) excitations is in stark contrast to behaviour in acceleration controlled (AC), or velocity controlled (VC), conditions, and is much easier to generalise. Although DC conditions are rare with buildings not exceeding 30 m in height displacement estimates based on such conditions can be taken as upper bound estimates in order that a conservative prediction of the displacement profile at the edge of a TU building can be obtained conveniently by the use of a constant amplification factor to scale results from planar analysis.

• Journal of Navigation and Port Research
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• v.39 no.4
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• pp.371-376
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• 2015

Port is responsible for the important role that creates a lot of value-added export and import-intensive countries, critical infrastructure, and in the national economy. Despite being an important facility for the past, awareness of the port is insufficient; In 2000s, increasing the world container traffic volumes, China's economic development, and trade volume in the Northeast Asia to generate a lot of are changing the perception of the role and importance of the port. According to the review of the master plan and the port recognition in Korean Port, this study examines determining factors which affects the port cargo volume. The target of the study is domestic small and medium-sized container port that receives a large hinterland cargo volume, excluding the impact of the Global Hub Port like Busan and Gwangyang port. Factors that affect the multiple regression analysis result of the port cargo volume are berthing capacity, degree of activation, connection number of countries, GRDP and number of manufacturers.

• Journal of Bio-Environment Control
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• v.9 no.2
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• pp.73-78
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• 2000

This study was carried out to investigate appropriate processing conditions for expanded rice hulls to be used as a medium material in nutrient cultures. The water holding capacity of expanded rice hulls produced by using a domestic grinder with 8 mm gap and 3 mm cutter height was 271.0, and the bulk density and CEC were 0.19g·m-3 and 37.0 cmol·kg-1, respectively. These values are higher than those of perilte. However, geometric mean diameter (GMD) of expanded rice hulls was 1.01mm which was smaller than that of perlite, indicating unfavorable porosity. After supplying nutrient solution, the faster water percolation in expanded rice than perlite required more frequent water supply. There was no significant difference in tomato fruit yield between expanded rice hull and perlite. The pH increase and the lack of nitrogen in early stage of culture are to be solved in the future.

• The Korean Journal of Applied Statistics
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• v.28 no.1
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• pp.103-114
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• 2015

The role of small and medium enterprises as an economic growth factor has been accentuated; consequently, the need to develop a business forecast model and indexes that accurately examine business situation of small and medium enterprises has increased. Most current business model and indexes concerning small and medium enterprises, released by public and private institutions, are based on Business Survey Index (BSI) and depend on subjective (business model and) indexes; therefore, the business model and indexes lack a capacity to grasp an accurate business situation of these enterprises. The business forecast model and indexes suggested in the study have been newly developed with Principal Component Analysis(PCA) and weight method to accurately measure a business situation based on reference dates addressed by the National Statistical Office(NSO). Empirical studies will be presented to prove that the newly proposed business model and indexes have their basis in statistical theory and their trend that resembles the existing Composite Index.

• Korean Journal of Applied Biomechanics
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• v.31 no.3
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• pp.162-167
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• 2021

Objective: This study aimed to identify the different wind speed categories and competitive level among windsurfers through GPS variables to provide the useful information on the development of training programs for enhancing windsurfers' performance. Method: Data from 69 female athletes who participated in 27 races during the 2018-2019 windsurfing season were used for the analysis. Average board speed, total race time, total distance, upwind race time, downwind race time, beam reach race time were collected through GPS. Unconfirmed data were excluded along with penalty point data. The wind conditions were classified as light, light to medium, medium, medium to heavy, and heavy wind, the competitive levels were classified as level 1, level 2, and level 3. Results: As for the average board speed, the level 1 or level 2 group showed higher board speed than the level 3 group in all wind conditions except for the light wind. The total race time and upwind race time showed less time in level 1 or level 2 group than level 3 group in all wind conditions. The total distance, downwind race time and beam reach race time showed less distance and time in level1 group than level 3 group under sufficient wind conditions. Conclusion: Our results show that the aerobic capacity to sustain pumping during upwind course in wind conditions below 15 kts effects performance. In wind conditions of 15 kts or more, indicated that the board control for the fast board speed and small distance required during up, down, beam reach courses had an effect on competition performance. This information can be provided to windsurfers and coaching as basic data for training programs to improve performance.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.299-306
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• 2021

Vitrification methods are commonly used for mammalian reproduction through the long-term storage of blastocyst produced in vitro. However, the survival and quality of embryos following vitrification are significantly low compared with blastocyst from in vitro production (IVP). This study evaluates that the survival of frozen-thawed bovine embryos was relevant to mitochondrial superoxide derived mitochondrial activity. Here we present supplementation of the cryopreservation medium with Mito-TEMPO (0.1 µM) induced a significant (p < 0.001; non-treated group: 56.8 ± 8.7%, reexpanded at 24 h vs Mito-TEMPO treated group: 77.5 ± 8.9%, re-expanded at 24 h) improvement in survival rate of cryopreserved-thawed bovine blastocyst. To confirm the quality of vitrified blastocyst after thawing, DNA fragmentation of survived embryos was examined by TUNEL assay. As a result, TUNEL positive cells rates of frozen-thawed embryos were lower in the Mito-TEMPO treated group (4.2 ± 1.4%) than the non-treated group (7.1 ± 3.5%). In addition, we investigated the intracellular ROS and mitochondrial specific superoxide production using DCF-DA and Mito-SOX staining in survived bovine embryos following vitrification depending on Mito-TEMPO treatment. As expected, intracellular ROS levels and superoxide production of vitrified blastocysts after cryopreservation were significantly reduced (p < 0.05) according to Mito-TEMPO supplement in freezing medium. Also, mitochondrial activity measured by MitoTracker Orange staining increased in the frozen-thawed embryos with Mito-TEMPO compared with non-treated group. These results indicate that the treatment of Mito-TEMPO during cryopreservation might induce reduction in DNA fragmentation and apoptosis-related ROS production, consequently increasing mitochondrial activation for developmental capacity of frozen-thawed embryos.

• Clinical and Experimental Vaccine Research
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• v.12 no.4
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• pp.328-336
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• 2023

Purpose: Human peripheral blood mononuclear cell (PBMC)-based in vitro systems can be of great value in the development and assessment of vaccines but require the right medium for optimal performance of the different cell types present. Here, we compare three commonly used media for their capacity to support innate and adaptive immune responses evoked in PBMCs by Toll-like receptor (TLR) ligands and whole inactivated virus (WIV) influenza vaccine. Materials and Methods: Human PBMCs were cultured for different periods of time in Roswell Park Memorial Institute (RPMI), Dulbecco's minimal essential medium (DMEM), or Iscove's modified DMEM (IMDM) supplemented with 10% fetal calf serum. The viability of the cells was monitored and their responses to TLR ligands and WIV were assessed. Results: With increasing days of incubation, the viability of PBMCs cultured in RPMI or IMDM was slightly higher than that of cells cultured in DMEM. Upon exposure of the PBMCs to TLR ligands and WIV, RPMI was superior to the other two media in terms of supporting the expression of genes related to innate immunity, such as the TLR adaptor protein gene MyD88 (myeloid differentiation factor 88), the interferon (IFN)-stimulated genes MxA (myxovirus resistance protein 1) and ISG56 (interferon-stimulated gene 56), and the leukocyte recruitment chemokine gene MCP1 (monocyte chemoattractant protein-1). RPMI also performed best with regard to the activation of antigen-presenting cells. As for adaptive immunity, when stimulated with WIV, PBMCs cultured in RPMI or IMDM contained higher numbers of IFNγ-producing T cells and secreted more immunoglobulin G than PBMCs cultured in DMEM. Conclusion: Taken together, among the different media assessed, RPMI was identified as the optimal medium for a human PBMC-based in vitro vaccine evaluation system.