Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Factors Affected on Plant Regeneration of Phyllitis scolopendrium (L.) Newm. In vitro

기내에서 변산일엽의 식물체 재생에 영향을 미치는 요인들

  • Jeong Jin-A (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Cheol-Hee (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
  • 정진아 (충북대학교 원예과학과, 생물건강산업개발연구센터) ;
  • 이철희 (충북대학교 원예과학과, 생물건강산업개발연구센터)
  • Published : 2006.04.01
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Abstract

This study was conducted to develop the efficient propagation method of fern Phyllitis scolopendrium using In vitro culture. The influence of the origin of the donor explant sources (rhizome, stipe, three parts of blade) and the homogenization of explants was investigated. Rhizome and stipe explants showed the organogenic capacity among the five explant sources and plant regeneration was promoted by homogenization of culture material. Optimum condition for vigorous and excellent growth of multiple shoots was the half-strength MS medium with 1% sucrose concentration. Generally, addition of $NaH_2PO_4$ to media enhanced shoot multiplication. The highest rate of shoot proliferation was observed on the media containing $5{\mu}M$ NAA. Also, combination of activated charcoal $(0.1{\sim}0.2%)$ and growth regulators to growth medium prevented the formation of multiple bud primordia, 'nodule'-like bud clusters and improved the normal morphogenesis of sporopytes in P. scolopendrium.

본 연구는 기내배양을 통하여 양치식물인 변산일엽의 효과적인 번식방법을 개발하기 위해 실시되었다. 절편의 기원(근경, 엽병, 엽신의 세 부분)과 절편의 균질화가 식물재생에 미치는 영향을 조사한 결과, 오직 근경과 엽병에서만 기관형성능력이 관찰되었으며, 식물체 재생은 절편을 균질화함으로써 촉진되었다. 다수의 신초가 활발한 생장을 보이는 적정조건은 sucrose 1%가 함유된 1/2MS 배지였다. 전반적으로 $NaH_2PO_4$의 첨가는 신초의 증식을 촉진하였으며, 가장 높은 신초증식률을 보인 것은 NAA $5{\mu}M$이 첨가된 배지였다. 또한 $0.1{\sim}0.2%$의 활성탄과 생장조절물질을 혼용 처리한 결과, 다수의 싹이 뭉쳐있는 듯한 'nodule'-like bud clusters의 생성을 억제하고 포자체의 정상적인 형태형성을 향상시켰다.

Keywords

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