Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Induction of Valiant of Cyrtomium caryoptideum var. coreanum Nakai by Chemical Mutagenesis In vitro and RAPD Analysis

기내에서 화학돌연변이원 처리에 의한 참쇠고비의 변이주 유기 및 RAPD 분석

  • Jeong Jin-A (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Cheol-Hee (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
  • 정진아 (충북대학교 원예과학과, 생물건강산업개발연구센터) ;
  • 이철희 (충북대학교 원예과학과, 생물건강산업개발연구센터)
  • Published : 2006.04.01
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Abstract

With the aim of inducing mutation in fern Cyrtomium caryoptideum var. coreanum, rhizome segments of In vitro-grown cultures were treated with chemical mutagens such as EMS, NMU and colchicine. Based on regeneration ratios, sensitivities for each treatments were assessed and also optimum treatment condition of each mutagens was explored. Optimum concentration for EMS treatment was considered to be 20 to 50mM and for NMU 5 to 10mM. NMU was found to be more effective in inducing chlorophyll and morphological variations than EMS. The RAPD were performed to check the genetic modification of phenotypical variants. As a result, polymorphic DNA band patterns between wild type and variants were observed by two 10-mer primers.

양치식물인 참쇠고비에서 인위적인 돌연변이를 유기하기 위하여, 기내배양한 근경조직에 EMS, NMU 등의 화학돌연변이원과 colchicine을 처리하였다. 식물체 재생률을 근거할 때, EMS의 적정 처리농도는 $20{\sim}50mM$이었으며, NMU는 $5{\sim}10mM$인 것으로 확인되었다. 또한 참쇠고비에서 엽의 색소 및 형태적 변이를 유도하는 데 있어 NMU가 EMS보다 효과적인 것으로 확인되었다. 표현형 변이 식물체의 유전적 변이를 분석하기 위하여 RAPD를 수행한 결과, 두 개의 10-mer primer에서 야생종과 변이주 사이에 다형성 DNA 밴드 패턴이 나타났다.

Keywords

References

  1. Avisian-Krechmer, O., Y. Eshdat, Y. Gueta-Dahan and G. Ben-Hayyim. 1999. Regulation of stress-induced phospholipid hydroperoxide glutathione peroxidase expression in Citrus. Planta 209: 469-477 https://doi.org/10.1007/s004250050750
  2. Bouman, H. and G.-J. De Klerk. 2001. Measurement of the extent of somaclonal variation in begonia plants regeneration under various condition. Comparison of three assays. Theor. Appl. Genet. 102: 111-117 https://doi.org/10.1007/s001220051625
  3. Chakraborti, S.P., K. Vijayan, B.N. Roy and S.M.H. Qadri. 1998. In vitro induction of tetraploidy in mulberry (Morus alba L.). Plant Cell Rep. 17: 799-803 https://doi.org/10.1007/s002990050486
  4. Coulibaly, L., B. Revol, M. Noirot, V. Poncet, M. Lorieux, C Carasco-Lacombe, J. Minier, M. Dufour and P. Hamon. 2003. AFLP and SSR polymorphism in a coffea interspecific backcross progeny ［(C. heterocalyx $\times$ C. canephora) $\times$ C. canephora ］. Theor. Appl. Genet. 107: 1148-1155 https://doi.org/10.1007/s00122-003-1355-4
  5. Devarumath, R.D., S. Nandy, V. Rani, S. Marimuthu, N. Muraleedharan and S.N. Raina. 2002. RAPD, ISSR, RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type) and C. assamica ssp. assamica (Assam-India type). Plant Cell Rep. 21: 166-173 https://doi.org/10.1007/s00299-002-0496-2
  6. DeVerno, L.L., Y.S. Park, J.M. Bonga and J.M. Barrett. 1999. Somaclonal variation in cryopreserved embryogenic clones of white spruce (Picea glauca (Moench) Voss.). Plant Cell Rep. 18: 948-953 https://doi.org/10.1007/s002990050689
  7. Doo, H.S., C.S. Kang and J.H. Ryu. 2001. Induction mutation by gamma-ray irradiation on crown bud of yacon (Polymnia sonchifolia Poeppig and Endlicher). Kor. J. Breed. 33: 1-6
  8. Farooqui, M.A., A.V. Rao, T. Jayasree and A. Sadanandam, 1997. Induction of atrazine resistance and somatic embryogenesis in Salanum melongena. Theor. Appl. Genet. 95: 702-705 https://doi.org/10.1007/s001220050615
  9. Fourre, J.-L., P. Berger, L. Niquet and P. Andre. 1997, Somatic embryogenesis and somaclonal variation in Norway spruce: Morphogenetic, cytogenetic and molecular approaches. Theor. Appl. Genet. 94: 159-169 https://doi.org/10.1007/s001220050395
  10. Gaj, M.D. 2002. Stimulation of somatic embryo formation by mutagens and darkness in culture of immature zygotic embryos of Arabidopsis thaliana (L.) Heynh. Plant Growth Regulation 37: 93-98 https://doi.org/10.1023/A:1020392014493
  11. Gueta-Dahan, Y., Z. Yaniv, A. zilinskas and G. Ben-Hayyim. 1997. Salt and oxidative stress: Similar and specific responses and their relation to salts tolerance in Citrus. Planta 203: 460-467 https://doi.org/10.1007/s004250050215
  12. Herring, A., D.L. Auld, M.D. Ethridge, E.F. Hequet and E. Bechere. 2002. Inheritance of fiber quality and lint yield in a chemically mutated population of cotton. Euphytica 136: 333-339 https://doi.org/10.1023/B:EUPH.0000032747.97343.54
  13. Hofmann, N.E., R. Raja, R.L. Nelson and S.S. Korban. 2004. Mutagenesis of embryogenic cultures of soybean and detecting polymorphisms using RAPD markers. Biological Plantarum 48: 173-177 https://doi.org/10.1023/B:BIOP.0000033441.46242.94
  14. Jones, D.L. 1987. Encyclopedia of ferns. Timer press, Portland
  15. Latado, R.R., A.H. Adames and A.T. Neto. 2004. In vitro mutation of chrysanthemum (Dendranthema grandiflora Tzvelev) with ethylmethane sulphonate (EMS) in immature floral pedicels. Plant Cell Tiss. Org. Cult. 77: 103-106 https://doi.org/10.1023/B:TICU.0000016481.18358.55
  16. Lee, B.C., T.S. Kwak, J.S. Park and K.W. Yu. 1997. Variation of agronomic characters and RAPD in induced boxthorn (Lycium chinense) mutants. Kor. J. Breed. 29: 453-460
  17. Lee, H.Y. and J.S. Jung and J.S. Lee. 1998. Induction of chlorophyll deficient mutant plant of Cymbidium kanran by EMS treatment. Kor. J. Plant Tiss. Cult. 25: 183-187
  18. Lee, I.S., D.S. Kim, C.P. Hong, S.Y. Kang, H.S. Song, S.J. Lee. Y.P. Lim and Y.I. Lee. 2003. Selection and characterization of S- aminoethyl-L-cysteine resistant from gamma-ray irradiated embryogenic callus in sweet potato. J. Plant Biotechnology 5: 233-238
  19. Lee, J.H. and S.Y. Lee. 2002. Selection of stable mutants from cultured rice anthers treated with ethyl methane sulfonic acid. Plant Cell Tiss. Org. Cult. 71: 165-171 https://doi.org/10.1023/A:1019957606600
  20. Lee, S.Y., J.K. Lee, T.H. Noh, H.J. Kang and S.Y. Lee. 1993. Variation of major characters in plants regenerated from rice cells treated with ethylmethane sulfonic acid. Kor. J. Plant Tiss. Cult. 20: 307-314
  21. Lim, J.D., M.J. Kim and C.Y. Yu. 2000. Induction and RAPD analysis of mutant plants by chemical mutagens in Gentiana axillariflora Leveille. Kor. J. Plant Tiss. Cult. 27: 89-94
  22. Lim, S.H., L.K.C. Looi, B.L. Ong and Y.C. Wee. 1997. A method of DNA isolation from epiphytic CAM ferns for use in random amplified polymorphic DNA analysis. Biologia Plantarum 39: 637-639 https://doi.org/10.1023/A:1000947319165
  23. Nakajima, Y., K. Oeda and T. Yamamoto. 1998. Characteriza tion of genetic diversity of nuclear and mitochondrial genomes in Daucus varieties by RAPD and AFLP. Plant Cell Rep. 17: 848-853 https://doi.org/10.1007/s002990050496
  24. Ong, B.L., S.H. Lim and K.C. Looi. 1998b. Morphological and molecular variations in the epiphytic CAM fern Pyrrosia piloselloides. Biologia Plantarum 41: 281-285 https://doi.org/10.1023/A:1001843403536
  25. Paek, K.Y. and K.R. Han. 1988. Micropropagation of gloxinia (Sinningia specipsa) from hypocotyl and cotyledon segments and treatment of EMS and colchicine on regenerated shoot tipes. J. Kor. Soc. Hort. Sci. 29: 126-135
  26. Pak, M.K. 1961. Flora of Korean Pteridophyta. Kyohakdoso Co., Seoul
  27. Pinheiro, A.A., M.T. Pozzobon, C.V. de Valle, M.I.O. Penteado and V.T.C. Carneiro. 2000. Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine. Plant Cell Rep. 19: 274-278 https://doi.org/10.1007/s002990050011
  28. Piqueras, A., J.A. Hernandez, E. Olmos, E. Hellin and F. Selvilla. 1996. Changes in antioxidant enzymes and organic solutes associated with adaptation of citrus cells to salt stress. Plant Cell Tiss. Org. Cult. 45: 53-60 https://doi.org/10.1007/BF00043428
  29. Predieri, S. 2001. Mutation indution and tissue culture in improving fruits. Plant Cell Tiss. Org. Cult. 64: 185-210 https://doi.org/10.1023/A:1010623203554
  30. Raimondi, J.P., R.W. Masuelli and E.L. Camadro. 2001. Assesment of somaclonal variation in asparagus by RAPD fingerprinting and cytogenic analyses. Sci. Hort. 90: 19-29 https://doi.org/10.1016/S0304-4238(00)00250-8
  31. Rao A.V., M.A. Farooqui and A. Sadanandam. 1997. Induction of lincomycine and streptomycin resistance by nitrosomethylurea and ethylmethanesulphonate in Capsicum annuum L. Plant Cell Rep. 16: 865-868 https://doi.org/10.1007/s002990050335
  32. Seong, N.S., C.H. Park, K.S. Kim, S.T. Lee and Y.H. Chang. 1995. In vitro variant induction and its content of gentiopicroside of Gentiana scabra Bunge. Kor. J. Med. Crop 3: 40-44
  33. Shim, K.K., Y.M. Ha and S.K. Lee. 1994. A study on the new yellow variegated cultivar of Korean Forsythia. J. Kor. Soc. Hort. Sci. 35: 270-278
  34. Thao, N.T., P.K. Ureshino, I. Miyajima, Y. Ozaki and H. Okubo. 2003. Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tiss. Org. Cult. 72: 19-25 https://doi.org/10.1023/A:1021292928295
  35. Triboush, S.O., N.G. Danienko, I.I. Ulticheva and O.G. Davydenko. 1999. Location of induced mutation and reversion in the chloroplast genome of Helianthus annuus. Plant Growth Reg. 27: 75-81 https://doi.org/10.1023/A:1006117114857
  36. Ude, G., M. Pillay, E. Ogundiwin and A. Tenkouano. 2003. Genetic diversity in an African plantain core collection using AFLP and RAPD markers. Theor. Appl. Genet. 107: 248-255 https://doi.org/10.1007/s00122-003-1246-8
  37. Xu, M.L., A.E. Melchinger, X.C. Xia and T. Lubberstedt. 1999. High-resolution mapping of loci conferring resistance to sugarcane mosaic virus in maize using RFLP, SSR and AFLP markers. Mol. Gen. Genet. 261: 574-581 https://doi.org/10.1007/s004380051003