• Title/Summary/Keyword: Colchicine

Search Result 105, Processing Time 0.131 seconds

Long-term Colchicine Prophylaxis on Operative Adhesion Formation in Embryo Transfer Donor Ewes and the Cytogenetic Evalution of Therapy (Embryo Transfer Donor Ewe에 생기는 수술상의 Adhesion 형성에 대한 장기간의 Colchicine 치료와 그에 따른 세포유전학적 분석)

  • 박석천
    • Korean Journal of Animal Reproduction
    • /
    • v.18 no.1
    • /
    • pp.63-70
    • /
    • 1994
  • Thirty ewes received typical trauma to their oviducts and uterine horns from surgical embryo collection procedures. Ten percent Dexamethasone was used as an irrigant on the exposed abdominal tissue prior to closing the incision. The treatment group received 17mg colchicine Om! lewe) and the control group was administered a 1.0ml placebo(PSS). Fifteen ewes that were initially treated with 17mg /im colchicine showed acute colchicine toxicity within 2-5 days after initial treatment and were removed from the study. Due to acute colchicine toxicity at 17mg, the colchicine level was lowered to 8, 4 and 2mg(4 ewes/group). Treatments consisted of daily injections of colchicine. One ewe in the 8mg group developed toxicity on day 5. Therefore, ewes were then administered colchicine every other day from day 6 to day 14 postsurgeryat 4 and 2 mg. the second laparotomy was performed 9 weeks after first treatment. Following second laparotomy, the treatment group(n=5) received 4 mg colchicine every day for 14 days and there was no clinical symptoms of colchicine toxicity. The third laparotomy was performed by the same operators 5 weeks after final treatment and the adhesions scored. Adhesion grading was based on a scale of 0-4, with 4 being the most severe. The results of adhesion grading(> 3) at second laparotomy were not significantly different(P>0.05)between the two groups. Adhesion formation observed at third laparotomy showed a reduced, but not significant reduction (P>0.05) in the colchicine-treated ewes when compared with the controls. Ten ewes(5 control and 5 treatment)were examined cytogenetically by bone marrow analysis five days post-treatment. There was no difference(P>0.05)in the incidence of numerical or structural aberrations between the two groups.

  • PDF

Effect of Colchicine on the Growth and Gravitropic Response via Ethylene Production in Arabidopsis Roots

  • Kim, Seon Woong;Park, Arom;Ahn, Dong Gyu;Kim, Soon Young
    • Korean Journal of Plant Resources
    • /
    • v.31 no.6
    • /
    • pp.597-603
    • /
    • 2018
  • Inhibitory effect of colchicine on growth and gravitropic responses in Arabidopsis root was explored to find whether there was an involvement of ethylene production. It has been known that cytoskeleton components are implicated in sedimentation of statoliths to respond to gravitropism and growth. The root growth was inhibited by 25% and 40% over control for 8 hr treatment of colchicine at a concentration of $10^{-5}M$ and $10^{-7}M$, respectively. The roots treated with colchicine at the concentration of $10^{-7}M$ showed the same pattern as control in 3 hr, however, gravitropic response was decreased in the next 5 hr. The colchicine treatment at the concentration of $10^{-5}M$ inhibited the gravitropic response resulting in $60^{\circ}$ of curvature. In order to better understand the role of colchicine, the production of ethylene was measured with and without the treatment of colchicine. Colchicine increased the ethylene production by 20% when compared to control via the activation of ACC oxidase and ACC synthase activity. These results suggest that the inhibition of the growth and gravitropic responses of Arabidopsis roots by the treatment of colchicine could be attributed to the rearrangement of microtubule, and increase of ethylene production.

A Fatal Case of Colchicine Poisoning (콜히친(Colchicine) 음독 후 사망한 환자 1례)

  • Ko, Seung Hyun;Lee, Gun Woo
    • Journal of The Korean Society of Clinical Toxicology
    • /
    • v.15 no.1
    • /
    • pp.51-55
    • /
    • 2017
  • Colchicine is a drug that has long been used to treat a variety of illnesses; however, it reportedly has adverse effects at apparent toxic doses as well as at lower and therapeutically recommended doses. The typical therapeutic dose of colchicine is up to 2.4 mg daily, although it is sometimes as high as 8-10 mg daily. Here, we describe a case in which the patient showed sudden deterioration and died because of unintentional colchicine poisoning with a relatively small dose. When a colchicine poisoned patient visits the hospital, the physician should identify the patient's colchicine poisoning dose and concomitant drugs. Moreover, the patients should be monitored intensively for 24 to 72 hours and managed with various supportive treatment methods early and actively.

Effect of Antimitotic Agent Colchicine on In Vitro Regeneration of Watermelon

  • Jaskani Muhammad J.;Raza H.;Khan M. M.;Kwon Sung W.
    • Journal of Plant Biotechnology
    • /
    • v.6 no.4
    • /
    • pp.247-252
    • /
    • 2004
  • In vitro cultures of watermelon were treated with antimitotic agent colchicine to induce ploidy alterations, particularly the induction of tetraploids. Explants cotyledon, embryonic end of seed, transverse sections of epicotyl and hypocotyl were cultured on MS media supplemented with BA ($1{\mu}M$) and colchicine ($0.01\%,\;0.05\%\;and\;0.1\%$). Explants were subcultured on colchicine free media after 4 and 7 days. Colchicine had negative effect on in vitro regeneration but this exhibited explants related response. However, hypocotyl section of seedlings induced maximum callus on $0.01\%$ colchicine. Shoot proliferation was more in cotyledon explants cultured on colchicine ($0.01\%$) for four days. Maximum root induction and root number were recorded in embryonic end explants. Overall, cotyledon and embryonic end explants, and low colchicine concentration ($0.01\%$) was found optimal in watermelon regeneration.

A Case of Colchicine-Induced Neutropenia in Liver Cirrhosis Patient (간경변 환자에서 콜히친 사용에 의한 호중구성 백혈구감소증 1예)

  • Lee, Ho-Chan;Lee, Heon-Ju;Kim, Yong-Gil;Park, Jae-Hyun;Kim, Sung-Joon;Park, Jun-Seok
    • Yeungnam University Journal of Medicine
    • /
    • v.26 no.2
    • /
    • pp.125-129
    • /
    • 2009
  • Colchicine is an alkaloid that has been used for treating acute gouty arthritis, psoriasis, scleroderma and Behcet's syndrome. Colchicine decreased liver fibrosis in rats with carbon tetrachloride induced cirrhosis and in patients with many liver diseases. Therapeutic oral doses of colchicine may cause nausea, vomiting, abdominal pain and diarrhea. The adverse effect of colchicine associated with the dose is bone marrow suppression, and especially neutopenia. Neutropenia has often been reported in patients have taken an overdose of colchicine. We describe a 64-year-old female liver cirrhosis patient with neutropenia that was induced by a therapeutic dose of colchicine.

  • PDF

Colchicine-Induced Polyploidy and It's Agronomic Characters in Bupleurum falcatum (배수체 작성에 따른 시호 작물 특성)

  • Son, Tae-Kwon;Lee, Sang-Chul;Chung, Il-Kyung
    • Korean Journal of Medicinal Crop Science
    • /
    • v.16 no.1
    • /
    • pp.39-43
    • /
    • 2008
  • The effect of colchicine treatment on the agronomic performance and polyploid formation of Bupleurum falcatum using flow cytometry technique was investigated. The roots of 4-leaf stage plants were treated with colchicine (0.5%) for 3, 6, 12, and 24 hours and then transplanted in the field. Agronomic characters (survival rate, plant height, chlorophyll content, bolting rate) were recorded at 4 weeks and 6 months after transplanting while flow cytometry technique was conducted for determination of polyploid formation. Flow cytometry technique revealed polyploid nuclear DNA formation in colchicine treated plants. The highest number of polyploids was obtained at the shortest colchicine treatment time indicating an inverse relationship between colchicine treatment time and polyploid formation. Results also showed that survival and bolting rates were inversely related with the treatment time while plant height and chlorophyll were not significantly affected by the treatment. This study showed a convenient method for determination of colchicine-induced polyploid in B. falcatum and its superior agronomic performance at shorter treatment time.

Improvement of Chromosome Doubling Efficiency in Cymbidium Hybrids by Colchicine and Oryzalin Treatment (Colchicine과 oryzalin 처리에 의한 Cymbidium hybrids의 기내 배수화 효율 증진)

  • Hwang, Sook-Hyun;Kim, Mi-Seon;Park, So-Young
    • Horticultural Science & Technology
    • /
    • v.33 no.6
    • /
    • pp.900-910
    • /
    • 2015
  • This study aimed to investigate the efficiency of colchicine and oryzalin in inducing polyploidy in two Cymbidium hybrids [Showgirl 'Silky' and Mystery Island 'Silk Road' (Silk Road-4)]. Colchicine was used at concentrations ranging from 50 to $500mg{\cdot}L^{-1}$, with treatments lasting 1 to 3 weeks. Oryzalin was used at concentrations ranging from 3 to $20mg{\cdot}L^{-1}$, with treatments lasting 3 to 6 days or 1 to 3 weeks. The survival rate of PLBs was better in colchicine than in oryzalin solutions. The ploidy levels were screened using flow cytometry. In C. Showgirl 'Silky', the highest chromosome doubling efficiencies were obtained with the 1-week treatment in $50mg{\cdot}L^{-1}$ colchicine (60%) and the 2-week treatment in $5mg{\cdot}L^{-1}$ oryzalin (46.7%). In C. Mystery Island 'Silk Road' (Silk Road-4), the highest chromosome doubling efficiencies were obtained with the 1-week treatment in $50mg{\cdot}L^{-1}$ colchicine (16.7%) and the 3-day treatment in $10mg{\cdot}L^{-1}$ oryzalin (6.7%). Colchicine was more efficient than oryzalin in terms of polyploidy induction. Furthermore, pre-treatment, which entailed poking 10 times with forceps, improved the efficiency of chromosome doubling.

Effects of Colchicine and EMS on Induction of Variants in Wasabia japonica MATSUM (Colchicine과 EMS 처리에 의한 고추냉이의 변이체 유도)

  • Park, Kie-In;Yoon, Myung-Ja;Kwon, Sung-Whan;Choi, Sun-Yong;Lee, Kang-Soo
    • Korean Journal of Medicinal Crop Science
    • /
    • v.4 no.1
    • /
    • pp.12-18
    • /
    • 1996
  • This study was investigated to improve the rate of the seed germination of Wasabia japonica with $GA_3$ and BAP. The germination rate was 36% with 100ppm $GA_3$ only and 32% with 10ppm BAP only, while that was 65% with combination of 100ppm $GA_3$ and 10ppm BAP compared to that of 7.5% in control. This results show us that the most effect of germination rate was with 100ppm $GA_3$ and 10ppm BAP. Mutagenesis was induced with mutagen, EMS and colchicine. Before the germination, the survival rate was 62% with 0.5% colchicine compared to that of 7% in control. After the germination, the 85% of developed seed was alive with treatment of 0.5% colchicine and 55% of those was alive with 0.06% EMS compared to that of 8% in control. We analyzed the karyotype and isozyme pattern of Wasabia japonica induced with colchicine and EMS. The tetratype of Wasabia japonica was observed from Wasabia japonica treated with 0.5% colchicine. whereas it was not observed with 0.06% EMS. The peroxidase pattern of colchicine treated Wasabia japonica was different from nontreatment. but that of EMS treated Wasabia japonica was the same with normal one.

  • PDF

Effects of Colchicine on Pulmonary Injury Induced by Paraquat (Colchicine이 Paraquat에 의한 폐상해에 미치는 영향)

  • Kim, Joong-Kil;Chang, Moo-Ung;Kwak, Jyung-Sik
    • Applied Microscopy
    • /
    • v.33 no.4
    • /
    • pp.275-282
    • /
    • 2003
  • This study was undertaken to investigate paraquat-induced pulmonary injuries and effects of colchicine on pulmonary fibrosis by paraquat. Fifteen Sprague-Dawley rats were intraperitoneally injected 10 mg/kg of paraquat and repeatedly with 2 days interval. Another 15 rats were injected paraquat as same manner and simultaneously injected 10 mg/kg of colchicine in a week. Five rats in each group were sacrificed 1, 2, and 4 weeks after initial injections, and lungs extracted were observed by light and electron microscopes. On light microscopy, there was mild infiltration of neutrophils, macrophages, and lymphocytes in alveolar spaces and walls at 1 week after paraquat injection. The cellularity of alveolar wall was increased with time. However, the cellularity was not so prominent in paraquat and colchicine simultaneously injected group. On electron microscopy, there was marked swelling or excoriation of type I epithelial cells and alveolar capillary endothelium with infiltration of neutrophils, macrophages and monocytes, and lymphocytes in alveolar walls. Such findings were persisted with time. In addition, fibroblastic proliferation and deposition of collagen fibers were prominent at 4 weeks after paraquat injection. Fibrosis also occurred at 4 weeks after paraquat and colchicine simultaneous injection. It was not proninent than that of paraquat injected group. According to the above result, it would be concluded that the type I pneumocytes and alveolar capillary endothelial cells are most vulnerable on paraquat poisoning, and that the colchicine is effective on inhibition of paraquat-induced pulmonary fibrosis.

The Modification of X-ray induced Mutation in Paramecium by Pre-and Post-irradiation Treatment with Colchicine. (X-선에 의한 돌연변이 빈도에 미치는 Colchicine의 영향)

  • 강영선;강현삼
    • The Korean Journal of Zoology
    • /
    • v.8 no.2
    • /
    • pp.33-36
    • /
    • 1965
  • We have carried out a study on the modification of the frequency of X-ray induced lethal and slow growing mutations by colchincine treatment before and after X-ray irradiation in Paramecium aurelia. 1. Lethal and slow-growing mutation induced by X-ray in Paramecium aurelia were reduced by colchicine treatment. 2. The effects of colchicine on the X-ray induced mutations were remarkable in the radiosensitive stages of cell division. 3. The pre-irradiation treatment with colchicine showed no significant influence on the frequency of mutations. 4. It is believed that the reduction of mutation induced by X-ray after treatment with colchicine was due to the fact that the delay of the cell division allowed more time for the pre-mutational damage to recover.

  • PDF