• Journal of Radiation Industry
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• v.5 no.2
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• pp.159-164
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• 2011

A variety of natural polymers have been used as tissue engineering scaffolds, drug delivery system, and cosmetic materials due to their higher biocompatibility and water uptake. As a major component of extracellular matrix, hyaluronic acid consisting of D-glucuronic acid and N-acetylglucosamine has been popularly used as a hydrogel material. Even though it has good properties to be used in the tissue engineering and cosmetic industry, its higher viscosity has limited a potential use in a variety of applications; only low content should be applied in preparing above products. In the present study, we investigated the effect of electron beam irradiation on the properties of hyaluronic acid. Hyaluronic acid paste containing low contents of water changed to solution after electron beam irradiation ranging from 1 to 10 kGy, which didn't exhibit any alteration of surface properties and morphological change after freeze-drying. However, its viscosity was significantly decreased as absorbed dose increased, which was approximately one by hundred in comparison with the viscosity of original hyaluronic acid solution with same concentration. In addition, it can still interact with positive charged chitosan generating polyelectrolyte complex. Therefore, only viscosity was decreased after electron beam irradiation, whereas other properties of hyaluronic acid maintained. Consequently, these hyaluronic acids with lower viscosities can be used in a variety of applications in tissue engineering, drug delivery, and cosmetic industry.

• Animal Bioscience
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• v.34 no.6
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• pp.975-984
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• 2021

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

• Ocean and Polar Research
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• v.43 no.4
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• pp.229-243
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• 2021

Quality control (QC) to process observed time series has become more critical as the types and amount of observed data have increased along with the development of ocean observing sensors and communication technology. International ocean observing institutions have developed and operated automatic QC procedures for these observed time series. In this study, the performance of automated QC procedures proposed by U.S. IOOS (Integrated Ocean Observing System), NDBC (National Data Buy Center), and OOI (Ocean Observatory Initiative) were evaluated for observed time-series particularly from the Yellow and East China Seas by taking advantage of a confusion matrix. We focused on detecting additive outliers (AO) and temporary change outliers (TCO) based on ocean temperature observation from the Ieodo Ocean Research Station (I-ORS) in 2013. Our results present that the IOOS variability check procedure tends to classify normal data as AO or TCO. The NDBC variability check tracks outliers well but also tends to classify a lot of normal data as abnormal, particularly in the case of rapidly fluctuating time-series. The OOI procedure seems to detect the AO and TCO most effectively and the rate of classifying normal data as abnormal is also the lowest among the international checks. However, all three checks need additional scrutiny because they often fail to classify outliers when intermittent observations are performed or as a result of systematic errors, as well as tending to classify normal data as outliers in the case where there is abrupt change in the observed data due to a sensor being located within a sharp boundary between two water masses, which is a common feature in shallow water observations. Therefore, this study underlines the necessity of developing a new QC algorithm for time-series occurring in a shallow sea.

• Korean Journal of Construction Engineering and Management
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• v.20 no.4
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• pp.104-113
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• 2019

Construction dispute about Extension of Time are complexly intertwined with various issues. The duration delay analysis defines the impact the issue has on the duration variation. And The international court and the arbitrator use the results of the analysis as a basis for reasonable duration judging. However, most of the domestic Construction sites have not established a business process for Extension of Time litigation. Therefore, Analysis data are collected after the occurrence of the dispute, there is not enough basic data of analysis almost. This study sought to improve the management efficiency by organizing information requirement for delay analysis and suggesting a reasonable business process. As a result of applying the proposed process to actual construction duration extension disputes, about 33% of practitioners, 46% of contractors, and 48% of legal advisors were satisfied with the process application site And Matrix validation was 91% identical. This study suggests that it is possible to increase the efficiency of the construction duration analysis work used as a basis in the construction dispute. Finally, the Computer based system design for this process should continue in the future.

• International Area Studies Review
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• v.17 no.4
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• pp.109-130
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• 2013

This study aims to identify the priority for the Gwangyang and Qingdao hinterlands which are in the same category of benchmarking crowed paths. This study has been mainly done with comparison. There is certain limitation to use competitive factors of existing research so, this study has had proper competitive factors deriving from factors analysis and studied hinterland priority of competing factors by AHP. Major results are as follows. First, the factor analysis resulted in 20 factor that were 0.6 or higher loading level of commonality and then these 20 factors were divided into groups: operating factors, service factors, cost factors, port infrastructure factors, and hinterland conditions factors with the rotated component matrix analysis. Second, according to the result of top competitive factors, the best factor was the hinterland condition(0.256). The other factors such as infrastructure, economy, accessibility, incentive, and port traffic in hinterland were highly ranked in terms of general importance using multiple weights. Third, the result of detailed properties importance about the final alternative, Gwangyang hinterland was considered more highly than Qingdao hinterland in the port information system, the support a variety of administrative services, the efficiency of the customs, and the tax benefits.

• Journal of the Korean Electrochemical Society
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• v.22 no.3
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• pp.87-103
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• 2019

Nonaqueous organic electrolyte solution in commercially available lithium-ion batteries, due to its flammability, corrosiveness, high volatility, and thermal instability, is demanding to be substituted by safer solid electrolyte with higher cycle stability, which will be utilized effectively in large-scale power sources such as electric vehicles and energy storage system. Of various types of solid electrolytes, composite solid electrolytes with polymer matrix and active inorganic fillers are now most promising in achieving higher ionic conductivity and excellent interface contact. In this review, some kinds and brief history of solid electrolyte are at first introduced and consequent explanations of polymer solid electrolytes and inorganic solid electrolytes (including active and inactive fillers) are comprehensively carried out. Composite solid electrolytes including these polymer and inorganic materials are also described with their electrochemical properties in terms of filler shapes, such as particle (0D), fiber (1D), plane (2D), and solid body (3D). In particular, in all-solid-state lithium batteries using lithium metal anode, the interface characteristics are discussed in terms of cathode-electrolyte interface, anode-electrolyte interface, and interparticle interface. Finally, current requisites and future perspectives for the composite solid electrolytes are suggested by help of some decent reviews recently reported.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.62-62
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• 2019

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1 and K2. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 pairedend read was used for GBS sequencing. GWAS was conducted using TASSEL 5.0. The TASSEL program uses a mixed linear model (MLM). The results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race and K2 resistant germplasm proportion remained between 66.67. The genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than 5 (K1 and K2) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. These SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races. The significant loci detected have also been illustrated and make the CPAS markers for NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.6
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• pp.55-69
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• 2018

As disaster risk and climate change volatility increase, there are more efforts to adapt to disasters such as forest fires, floods, and landslides. Most of the research, however, is about influence of human activities on disaster and there is few research on disaster adaptation for species. Previous studies focusing on biodiversity in selecting conservation areas have not addressed threats of disaster in the habitats for species. The natural disasters sometimes play role of drivers of ecological successions in the long run, but they might cause serious problems for the conservation of vulnerable species which are endangered. The purpose of this study is to determine whether soil loss (SL) is effective in selecting habitat management areas for amphibians and reptiles. RUSLE model was used to calculate soil loss (SL) and the distribution of each species (SD) was computed with MaxEnt model to find out the biodiversity index. In order to select the habitat management area, we estimated the different results depending if value of soil loss was applied or not by using MARXAN, a conservation priority selection tool. With using MARXAN, conservation goals can be achieved according to the scenario objectives, and the study has been made to meet the minimum habitat area. Finally, the results are expressed in two; 1) the result of soil loss and biodiversity with MATRIX method and 2) the result of regional difference calculated with MARXAN conservation prioritization considering soil loss. The first result indicates that the area with high soil loss and low species diversity have lower conservation values and thus can be managed as natural disturbances. In the area where soil loss is high and species diversity is also high, it becomes where a disaster mitigation action should be taken for the species. According to the conservation priorities of the second result, higher effectiveness of conservation was obtained with fewer area when it considered SL in addition to SD, compared to when considered only biodiversity. When the SL was not taken into consideration, forest area with high distribution of species were important, but when SL considered, the agricultural area or downstream of the river were represented to be a major part of habitats. If more species data or disaster parameters other than soil loss are added as variables later, it could contribute as a reference material for decision-making to achieve various purposes.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.21 no.3
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• pp.183-191
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• 2021

Non-intrusive load monitoring is a technology that can be used for predicting and classifying the type of appliances through real-time monitoring of user power consumption, and it has recently got interested as a means of energy-saving. In this paper, we propose a system for classifying appliances from user consumption data by combining GAF(Gramian angular field) technique that can be used for converting one-dimensional data to the two-dimensional matrix with convolutional neural networks. We use REDD(residential energy disaggregation dataset) that is the public appliances power data and confirm the classification accuracy of the GASF(Gramian angular summation field) and GADF(Gramian angular difference field). Simulation results show that both models showed 94% accuracy on appliances with binary-state(on/off) and that GASF showed 93.5% accuracy that is 3% higher than GADF on appliances with multi-state. In later studies, we plan to increase the dataset and optimize the model to improve accuracy and speed.

• Clinical and Experimental Reproductive Medicine
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• v.48 no.2
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• pp.111-123
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• 2021

Objective: Using domestic cats as a biomedical research model for fertility preservation, the present study aimed to characterize the influences of ovarian tissue encapsulation in biodegradable hydrogel matrix (fibrinogen/thrombin) on resilience to cryopreservation, and static versus non-static culture systems following ovarian tissue encapsulation and cryopreservation on follicle quality. Methods: In experiment I, ovarian tissues (n=21 animals; 567 ovarian fragments) were assigned to controls or hydrogel encapsulation with 5 or 10 mg/mL fibrinogen (5 or 10 FG). Following cryopreservation (slow freezing or vitrification), follicle viability, morphology, density, and key protein phosphorylation were assessed. In experiment II (based on the findings from experiment I), ovarian tissues (n=10 animals; 270 ovarian fragments) were encapsulated with 10 FG, cryopreserved, and in vitro cultured under static or non-static systems for 7 days followed by similar follicle quality assessments. Results: In experiment I, the combination of 10 FG encapsulation/slow freezing led to greater post-thawed follicle quality than in the control group, as shown by follicle viability (66.9%±2.2% vs. 61.5%±3.1%), normal follicle morphology (62.2% ±2.1% vs. 55.2%±3.5%), and the relative band intensity of vascular endothelial growth factor protein phosphorylation (0.58±0.06 vs. 0.42±0.09). Experiment II demonstrated that hydrogel encapsulation promoted follicle survival and maintenance of follicle development regardless of the culture system when compared to fresh controls. Conclusion: These results provide a better understanding of the role of hydrogel encapsulation and culture systems in ovarian tissue cryopreservation and follicle quality outcomes using an animal model, paving the way for optimized approaches to human fertility preservation.