• 대한기계학회논문집B
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• 제34권3호
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• pp.251-258
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• 2010

본 논문은 다기능 미소유체시스템의 일체형 패키징을 위한 MSI (microfluidic system interface) 기술을 제안하고, 이를 설계, 제작, 시험 평가하였다. MSI 기술을 통해 플러그 방식의 유체 인터커넥터, 유체제어를 위한 미소밸브, 광학 인터페이스를 위한 광학창을 유체시스템에 일체형으로 쉽게 구현할 수 있었다. MSI 기술의 유용성을 보이기 위해 미소 유전자시료전처리시스템에 적용되었으며, 미소 유전자시료전처리시스템은 세포정제, 세포분리, 세포용해, DNA 고체상추출, 중합효소연쇄반응, 그리고 모세관전기영동 기능으로 구성되었다. 나아가 MSI 기술이 적용된 미소 유전자시료전처리시스템의 DNA 고체상추출 및 중합효소연쇄반응의 실험결과로부터 MSI가 미소유체시스템을 위한 실용적 패키징 기술임이 검증되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2393-2399
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• 2013

Objective: Mitochondrial DNA (mtDNA) is considered a hotspot of mutations in various tumors. However, the relationship between microsatellite instability (MSI) and mtDNA copy number alterations in lung cancer has yet to be fully clarifieds. In the current study, we investigated the copy number and MSI of mitochondrial genome in lung carcinomas, as well as their significance for cancer development. Methods: The copy number and MSI of mtDNA in 37 matched lung carcinoma/adjacent histological normal lung tissue samples were examined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) assays for sequence variation, followed by sequence analysis and fluorogenic 5'-nuclease real-time PCR. Student's t test and linear regression analyses were employed to analyze the association between mtDNA copy number alterations and mitochondrial MSI (mtMSI). Results: The mean copy number of mtDNA in lung carcinoma tissue samples was significantly lower than that of the adjacent histologically normal lung tissue samples (p<0.001). mtMSI was detected in 32.4% (12/37) of lung carcinoma samples. The average copy number of mtDNA in lung carcinoma samples containing mtMSI was significantly lower than that in the other lung carcinoma samples (P<0.05). Conclusions: Results suggest that mtMSI may be an early and important event in the progression of lung carcinogenesis, particularly in association with variation in mtDNA copy number.

• 대한종양외과학회지
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• 제9권2호
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• pp.80-86
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• 2013

Purpose: Hypermethylation of human mut L homologue 1 (hMLH1) promoter region is known to cause sporadic microsatellite instability (MSI) colorectal cancers. 5,10-methylenetetrahydrofolate reductase (MTHFR) is the key enzyme in folate metabolism, acting as a methyl donor for DNA methylation. In this study, we investigate whether the polymorphism of MTHFR 677C>T plays a role in the alteration of the promoter-specific hypermethylation, predisposing to MSI colorectal cancers. Methods: Total of 487 sporadic colorectal cancer patients in CHA Bundang Medical Center were collected. MSI was identified when two or more are positive among five microsatellite markers (BAT25, BAT26, D17S250, D5S346, D2S123). The others were classified as microsatellite stable (MSS). Polymorphism of MTHFR 677C>T was genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: MSI was observed in 65 of 487 patients (12.73%). MSI colorectal cancers showed similar clinicopathological features with previously reported; younger age onset, right-sided preponderance, mucinous and poorly differentiated histology, lower stage, fewer lymph node metastases than MSS tumors (each P<0.05). The frequency of MTHFR 677TT genotype was 17.7% in the MSI group higher than 14.6% in the MSS group (P=0.17). Although not statistically significant, compared to the MTHFR 677CC referent, MTHFR 677 CT+TT genotype was more likely to have MSI than MSS (odds ratio, 1.81; 95% confidence interval, 0.94 to 3.68; P=0.06). Conclusion: This study demonstrated higher frequency of MTHFR 677TT genotype in MSI colorectal cancers. Furthermore, individuals with MTHFR 677CT+TT variant type might potentially develop MSI rather than MSS colorectal cancers.

• 한국통신학회논문지
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• 제25권10A호
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• pp.1492-1498
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• 2000

Sine 보간법을 이용한 PSAM은 Cavers의 최적 Wiener 필터를 사용한 알고리즘보다 구현은 간단하면서 채널추정 성능은 거의 동일하다. 그러나 이 알고리즘은 window 함수와 같이 사용해야만 우수한 채널추정 성능을 갖는다. 본 논문에서는 이러한 기존의 sine 보간법 (conventional sine interpolation : CSI) 의 단점을 보완한 수정된 sine 보간법 (modified sine interpolation : MSI) 을 제안한다. MSI 알고리즘의 성능평가를 위해 다중반송파 QAM 시스템과 주파수 선택성 페이딩을 고려한 최적 프레엄구조를 사용하였다. 성능평가에서 window 함수를 사용하지 않은 MSI 알고리즘은 더 적은 수의 파일럿 심볼을 사용하면서도, window 함수를 사용한 CSI 알고리즘과 거의 동일한 BER 성능을 보였다. 아울러, window 함수를 사용한 MSI 알고리즘의 성능은 PSAM을 적용한 이론적인 16QAM의 성능과 거의 일치하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권4호
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• pp.337-344
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• 2000

본 연구에서는 MSI와 구강암과의 상관관계를 규명하기 위하여 17례의 구강암에 대하여 12종류의 marker를 이용하여 MSI 빈도를 조사하였으며, 동시에 p53단백의 과발현과 유전자 돌연변이 양상에 대해서도 알아보았다. 그 결과 4종류 이상의 marker에 대해서 MSI가 나타나는 widespread MSI의 경우 임상병리학적으로 뚜렷한 특징이 없었다. 또한 흡연과 MSI 빈도간에도 연관성이 없었으나 흡연은 p53 유전자의 돌연변이를 증가시켜 암화과정을 촉진하는 작용을 하는 것으로 나타났다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권9호
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• pp.4259-4265
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• 2016

Background: Colorectal malignancies with high microsatellite instability (MSI-H), either hereditary (Lynch syndrome) or sporadic, demonstrate better prognosis and altered response to 5FU chemotherapy. It is now recommended to perform MSI testing for all new cases of colorectal cancer regardless of being categorized as hereditary or sporadic. For MSI detection, immunohistochemistry or PCR-based protocols using a cohort of various sets of STR markers are recommended. Here we aimed to evaluate a simplified protocol using just a single STR marker, MT1XT20 mononucleotide repeat, for detection of MSI in Lynch syndrome patients. A Promega five-marker MSI testing panel and immunohistochemistry (IHC) were used as the gold standard in conjunction with MT1XT20. Materials and Methods: Colorectal patients with a positive history of familial cancers were selected by evaluating medical records. Based on Amsterdam II criteria for Lynch syndrome 20 families were short listed. DNA was extracted from formalin fixed paraffin embedded tumour and adjacent normal tissues resected from the index case in each family. Extracted DNA was subjected to MT1XT20 mononucleotide marker analysis and assessment with a commercially available five marker MSI testing kit (Promega, USA). IHC also was performed on tissue sections and the results were compared with PCR based data. Results: Eight (40%), seven (35%) and five (25%) cases were MSI positive using with the Promega kit, IHC and MT1XT20, respectively. Among the markers included in Promega kit, BAT26 marker showed instability in all 8 samples. NR24 and NR21 markers showed instability in 7 (87.5%), and BAT25 and MONO 27 in 6 (75%) and 5 (62.5%). Conclusions: Although MT1XT20 was earlier reported as a valid standalone marker for MSI testing in CRC patients, we could not verify this in our Iranian patients. Instead BAT26 among the markers included in Promega MSI testing kit showed instability in all 8 MSI-H CRC samples. Therefore, it seems BAT26 could act well as a single marker for MSI testing in Iranian CRC patients.

• The Journal of Asian Finance, Economics and Business
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• 제8권3호
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• pp.307-318
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• 2021

The objective of this study is to analyze the performance of Islamic banks with the Maqashid Shariah approach. The analysis technique used is the Simple Additive Weighting Method (SAW) to solve multi-attribute decision problems. The sampling technique used was purposive sampling while the data came from the annual report of each bank. The results showed that the BTPN Shariah (BTPNS) and Bank Muamalat Indonesia (BMI) are ranked first and second respectively on the Maqashid Shariah Index (MSI) with values of 0.265429 and 0.237110 respectively. Panin Dubai Shariah Bank (PDSB) ranked third with an MSI value of 0.180733, followed by BCA Shariah which ranked fourth with an MSI value of 0.151299. BRI Shariah ranked fifth with an MSI value of 0.128606, followed by BNI Shariah which ranked sixth with an MSI value of 0.124661. Bank Mega Shariah ranked last with an MSI value of 0.087068. Furthermore, there is a relationship (correlation) between ROE, ROA, and OEOI and MSI since each data has a value of 0.000, 0.000, 0.050, and 0.001 respectively, which is smaller than the significance value of 0.05. On the other hand, NPF, TPF, and Asset Growth Rates do not correlate with the MSI since each data has a value of 0.051, 0.252, and 0.215 respectively which is greater than the significance value of 0.05.

• Mass Spectrometry Letters
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• 제11권3호
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• pp.41-51
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• 2020

Microbes influence many aspects of human life from the environment to health, yet evaluating their biological processes at the chemical level can be problematic. Mass spectrometry imaging (MSI) enables direct evaluation of microbial chemical processes at the atomic to molecular levels without destruction of valuable two-dimensional information. MSI is a label-free method that allows multiplex spatiotemporal visualization of atomic- or molecular-level information of microbial and microberelated samples. As a result, microbial MSI has become an important field for both mass spectrometrists and microbiologists. In this review, basic techniques for microbial MSI, such as ionization methods and analyzers, are explored. In addition, we discuss practical applications of microbial MSI and various data-processing techniques.

• Mass Spectrometry Letters
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• 제14권3호
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• pp.57-78
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• 2023

Understanding the chemical composition of the brain helps researchers comprehend various neurological processes effectively. Understanding of the fundamental pathological processes that underpin many neurodegenerative disorders has recently advanced thanks to the advent of innovative bioanalytical techniques that allow high sensitivity and specificity with chemical imaging at high resolution in tissues and cells. Mass spectrometry imaging [MSI] has become more common in biomedical research to map the spatial distribution of biomolecules in situ. The technique enables complete and untargeted delineation of the in-situ distribution characteristics of proteins, metabolites, lipids, and peptides. MSI's superior molecular specificity gives it a significant edge over traditional histochemical methods. Recent years have seen a significant increase in MSI, which is capable of simultaneously mapping the distribution of thousands of biomolecules in the tissue specimen at a high resolution and is otherwise beyond the scope of other molecular imaging techniques. This review aims to acquaint the reader with the MSI experimental workflow, significant recent advancements, and implementations of MSI techniques in visualizing the anatomical distribution of neurochemicals in the human brain in relation to various neurogenerative diseases.

• 대한전자공학회논문지TC
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• 제44권10호
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• pp.99-109
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• 2007

무선랜에서 QoS를 지원하기 위해 제안된 IEEE 802.l1e의 HCCA (HCF Controlled Channel Access)는 폴링에 의한 채널 제어 방식이다. 즉 HCCA는 중앙 제어 방식이기 때문에 자원을 분배하기 위한 스케줄링 알고리즘이 필요하며 IEEE 802.11e 표준에는 Reference 스케줄러가 제시되어 있다. Reference 스케줄러븐 폴링 리스트에 있는 모든 스테이션들을 동일한 주기로 폴링하기 때문에 잦은 폴링으로 인해 오버헤드 면에서 비효율적이며, TXOP (Transmission Opportunity)의 활용률 면에서도 낮다. 본 논문에서는 이러한 단점을 극복하고자 스테이션들의 MSI (Maximum Service Interval)에 따라 폴링 주기를 단계별로 나누어서 스케줄링하기 위한 트래픽 스케줄링 및 수락 제어 (admission control) 알고리즘을 제안한다. 제안하는 알고리즘에서는 스테이션들이 자시의 MSI와 가능한 비슷한 주기로 폴링되기 때문에 Reference 스케줄러에 비해 폴링 오버헤드가 감소하게 되며, TXOP 활용률도 증가하게 된다. 시뮬레이션을 통한 Reference 스케줄러와의 성능 비교는 제안된 알고리즘이 전체적인 전송률 면에서 더 높은 성능을 보이고 더 많은 수의 스테이션이 서비스가 가능함을 보여준다.