Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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MICROSATELLITE INSTABILITY AND p53 GENE MUTATION IN ORAL SQUAMOUS CELL CARCINOMA

구강편평상피세포암 조직에서의 Microsatellite 불안정성 및 p53 유전자 돌연변이 분석

  • Choi, Tae-Ho (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University) ;
  • Chung, Woon-Bok (Department of Dental Microbiology, College of Dentistry, Kyungpook National University) ;
  • Hong, Su-Hyung (Department of Dental Microbiology, College of Dentistry, Kyungpook National University) ;
  • Kim, Jin-A (Department of Dental Microbiology, College of Dentistry, Kyungpook National University) ;
  • Na, Sun-Oung (Department of Dental Microbiology, College of Dentistry, Kyungpook National University) ;
  • Jang, Hyun-Jung (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University) ;
  • Sohn, Yoon-Kyung (Department of Pathology, College of Medicine, Kyungpook National University) ;
  • Kim, Chin-Soo (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University) ;
  • Kim, Jung-Wan (Department of Dental Microbiology, College of Dentistry, Kyungpook National University)
  • 최태호 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 정운복 (경북대학교 치과대학 구강미생물교실) ;
  • 홍수형 (경북대학교 치과대학 구강미생물교실) ;
  • 김진아 (경북대학교 치과대학 구강미생물교실) ;
  • 나선영 (경북대학교 치과대학 구강미생물교실) ;
  • 장현중 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 손윤경 (경북대학교 의과대학 병리학교실) ;
  • 김진수 (경북대학교 치과대학 구강악안면외과학교실) ;
  • 김정완 (경북대학교 치과대학 구강미생물교실)
  • Published : 2000.08.31
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Abstract

Germ-line mutations at DNA repair loci confer susceptibility to colon cancer in hereditary non-polypopsis colorectal cancer. Somatic loss of DNA mismatch repair gene has been reported in a large variety of other tumor types. Replication errors(RERs) judged by microsatellite instability(MSI) and its associated mutations have been recognized as an important mechanism in various tumor types. To investigate associations between MSI and oral squamous cell carcinoma, the frequency of MSI using 12 microsatellite markers were analyzed for the series of oral tumors. Of 17 tumors, 8 cases(47%) did not show instability at any of the 12 loci; 5(29%) showed instability at $2{\sim}3$ loci; and 4(24%) showed instability above 4 loci. The 4 cases showing widespread MSI did not differ from those without evidence of instability in terms of age at diagnosis, degree of differentiation, metastasis to lymph node, tumor location or the presence of mutations in the p53 tumor suppressor gene. DCC and D17S 796 were the most frequently detected in MSI analysis. There were no correlation between smoking and MSI frequency, instead, smoking was suggested to increase the mutation rate of p53 and development of oral carcinomas.

본 연구에서는 MSI와 구강암과의 상관관계를 규명하기 위하여 17례의 구강암에 대하여 12종류의 marker를 이용하여 MSI 빈도를 조사하였으며, 동시에 p53단백의 과발현과 유전자 돌연변이 양상에 대해서도 알아보았다. 그 결과 4종류 이상의 marker에 대해서 MSI가 나타나는 widespread MSI의 경우 임상병리학적으로 뚜렷한 특징이 없었다. 또한 흡연과 MSI 빈도간에도 연관성이 없었으나 흡연은 p53 유전자의 돌연변이를 증가시켜 암화과정을 촉진하는 작용을 하는 것으로 나타났다.

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