• 대한치과보철학회지
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• 제42권3호
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• pp.261-266
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• 2004

Statement of problem. The effects of surface roughness have not or insufficiently been analyzed on earlier events such as cell adhesion though cell behavior most germane to implant performance is cell adhesion. Purpose. The purpose of this study was to evaluate cell adhesion of osteoblast-like cells (MG63) onto three types of titanium disks with varying roughness using the Elisa assay. Materials and methods. Representative disks from each group (SLA, HA, machined) were subjected to surface analysis and surface roughness was measured by the optical interferometer (Accura 2000, Intekplus Co., Seoul, Korea). Following this, MG63 cells were cultured on the titanium disks and released. Cell adhesion measurements using the Elisa assay were performed specifically at three points: after 24, 48, and 72 hours of culture. Results. Among the 3 types of surface analyzed, the SLA surface was the roughest with a Ra value of $1.114{\mu}m$ followed by HA coated surface and machined surface, consecutively. The optical density values for the SLA surface group was significantly higher than that of the machined and HA coated surface groups following 24 and 48 hours of culture. The cell culture on HA coated surface showed significantly higher values compared to the machined surface following 24, 48 and 72 hours of culture. Conclusion. The results suggest that surface treatment of titanium surfaces enhanced cell adhesion of human osteoblast-like cells (MG63).

• 동아시아식생활학회지
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• 제23권1호
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• pp.39-43
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• 2013

Carthamus tinctorius L. and Eucommia umoides Oliver are often used in traditional herbal medicines for reducing damage to the liver, kidney, bone and muscle. In the present study, we investigated cell viability and alkaline phosphatase activity in the human osteoblast-like MG-63 cell line with methanol extracts of Carthami Semen (CS) and Eucommiae Cortex (EC) alone or in a mixture (CS+EC). Osteoblast cell viability was evaluated using the MTS assay and alkaline phosphatase activity assays. The cell viability and alkaline phosphatase activity significantly increased in MG-63 osteoblast cells treated with the CS+EC mixture. These findings suggest the CS+EC mixture may have beneficial effects on bone health through the proliferation of osteoblast cells.

• 치위생과학회지
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• 제14권2호
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• pp.101-106
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• 2014

본 연구는 저 출력 초음파 장치를 이용하여 osteoblastlike MG-63 세포에 1 MHz, 3 Mz 강도의 초음파 처리 후 조골세포의 변화를 관찰하였다. 3 MHz 강도의 주파수를 osteoblast-like MG-63 세포에 처리한 결과 7일 후 성장률이 대조군보다 증가하였다. 저 출력 초음파가 골의 치유에 영향을 미치는 인자를 확인하였을 때 ALP, osteocalcin, VEGF, colla 1A1의 증가를 확인하였으며, 1 MHz 주파수 처리시 integrin alpha 2의 발현이 대조군과 유사하게 발현되는 것을 확인하였다. 반면에, osteonectin, osteopontin, fibronectin, MMP 2 등은 변화가 없음을 확인하였다. 그러므로 본 연구는 저 출력 초음파 장치는 뼈 치유 촉진 단백질들의 발현을 증가시켜 구강 내 외과적 수술 시 골 치유 촉진 효과에 대한 가능성을 제시하였으며 임상에서 골 치유 촉진을 위한 장비로서의 사용 가능성을 제시하였다고 생각된다.

• Restorative Dentistry and Endodontics
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• 제35권3호
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• pp.222-228
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• 2010

본 연구의 목적은 현재 치근단 역충전재로 널리 사용되고 있는 MTA와 새롭게 개발된 제품인 DB를 MG63 세포를 사용하여 비교하는 것이다. 치근단 역충전재료로는 MTA, DB, IRM을 사용하였고 대조군으로는 tissue culture plastic을 사용하였다. 각 재료를 혼합하고 혼합물의 경화가 일어나도록 24시간 동안 놓아두었다. MG63 세포를 각 군에 뿌려준 후 세포가 재료에 부착될 수 있도록 4시간 배양하였다. 치근단 역충전재를 세포에 접촉시킨 후 세포수준의 초기 반응을 관찰하였다. 12시간 더 배양한 후 세포가 각 재료에 붙어 있는 정도를 관찰하고, 각 재료가 골형성에 미치는 영향을 알아보기 위해 ELISA를 이용하여 $TGF{\beta}1$, OC를 측정하였고 ALP의 양도 측정하였다. 결과는 일원배치분산분석법으로 통계처리하였다. 그 결과, 재료에 부착이 일어난 세포의 수 항목과 OC 항목에서만 MTA와 DB간에 통계적으로 차이가 없었다. 다른 항목들에서는 모든 군 간에 통계적으로 유의한 차이가 있었다. DB가 MTA와 완전히 같은 세포반응을 보이지는 않았지만 부착이 일어난 세포의 수는 재료의 생체적합성을 나타내며 OC는 골형성 정도를 나타내므로 DB가 역충전 재료로 사용된다면 MTA와 유사한 결과를 보일 것으로 예측된다.

• 생약학회지
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• 제49권1호
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• pp.28-39
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• 2018

This study investigated the effect of snail extract on the growth parameters of old female rats (27 weeks). Rats were administered orally with snail extract at a dose of 100 mg/kg, 200 mg/kg, chondroitin sulfate 10 mg/kg and 0.9% saline (control) for 8 weeks. Bone mineral density (BMD) and serum concentrations of insulin-like growth factor 1 (IGF-1) and insulinlike growth factor-binding protein 3 (IGFBP-3) were significantly higher in rats exposed to snail extract for 8 weeks. MG-63 cells (human osteoblast-like cells) were treated with snail extract for 48 h. Their differentiation and proliferation was investigated with Western blot and morphological changes observed via immunofluorescence staining of ${\beta}-catenin$. Treatment with snail extract significantly increased the levels of growth factors including ${\beta}-catenin$ and IGF-1. The snail extract affected osteoblast formation. Morphological changes in MG-63 cells were observed via immunofluorescence staining. Treatment with snail extract increased the expression of ${\beta}-catenin$ in MG-63 cells. Results suggest that the treatment of MG-63 cells with snail extract increased the longitudinal growth and growth factor levels. Snail extract may be pharmacologically effective in osteogenic differentiation in vitro and represents a potential therapeutic agent for bone formation.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.387.1-387.1
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• 2002

Aging and estrogen cleficiency after menopause induce bone loss and result in osteoporosis. This study was investigated effects of n-hexane fraction (Hx) extracted from Astragali Radix on osteoporosis with osteoblast-like cell line (MG-63 and Saos-2) and an ovariectomized (OVX) rat model. Proliferation of osteoblast-like cells. MG-63 and Saos-2. was tested with MTT and alkaline phosphatase (ALP) assays. (omitted)

• Molecular & Cellular Toxicology
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• 제4권3호
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• pp.192-198
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• 2008

A variety of titanium (Ti) and its alloys are used in the clinical procedures of bone regeneration for periodontal and dental implant therapies. This study was performed to determine the effect of different surface dental implant materials on biologic responses of a MG-63 human osteoblast-like cell line. MG-63 cells were cultured on Ti coated with hydroxyapatite (HA), calcium metaphosphate (CMP), anodized (A), which compared with non-coated Ti (control). The appearances of surface of dental implant materials and the morphology of these cells were assessed by scanning electron microscopy (SEM). The gene expression profiles of MG-63 cells cultured on Ti were examined by human cDNA microarray (1,152 elements). The expression of several genes was up- and down-regulated by different surfaces of dental implant materials. Interesting, the genes correlated with cellular adhesion and extra cellular matrix (ECM) formation were enhanced, in accordance surface morphology of the dental implant materials used.

• 대한치과교정학회지
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• 제34권6호
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• pp.514-525
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• 2004

흡연이 골형성 세포의 기능을 억제한다는 것은 이미 잘 알려져 있으나 대다수의 연구가 주로 역학조사에 기초하여 이루어져 왔다. 본 연구의 목적은 흠연과 골형성 세포의 상관관계를 실험적으로 평가하기 위한 것이다. 본 연구를 위해 인간 골육종 세포인 MG63 조골제포주를 이용하였으며, 니코틴이 조골세포의 증식, 염기성 인산분해효소의 활성, 오스테오칼신곽 오스테오프로테제린의 생성 그리고 mRNA발현에 미치는 영향을 관찰하였다 MG63조골세포를 니코틴 농도가 각각 $1.0{\mu}M$,\;1.0mM, 2.5mM, 5.0mM, 7.5mM, 그리고 10.0mM,이 함유된 배지에서 1일, 2일, 3일 그리고 6일 동안 배양 실험을 하여 다음과 같은 결과를 얻었다. MG63 조골세포의 증식이 저농도의 니코틴에서는 일시적으로 활성화되었지만 5.0mM 이상의 고농도에서는 억제되었다. 염기성 인산분해효소의 활성은 니코틴 농도가 증가함에 따라 감소하였다. 오스테오칼신 생성양은, 배양 1일 후, 5.0mM 이하의 농도에서 증가하였으나 7.5mM 이상의 고농도에서는 감소하였다. MG63 조골세포를 3일 배양한 경우, 오스테오칼신 생성양은 1.0mM의 저농도에서도 감소하였다 니코틴이 오스테오칼신 단백질 생성과 오스테오칼신 mRNA생성에 미치는 영향은 1일과 3일에서 다소 차이가 있었다. 오스테오프로테제린의 생성양은 모든 실험군에서 니코틴을 함유하지 않은 대조군보다 감소하였다. 하지만 mRNA 수치는 단백질 생성과 상반되게 7.5mM (3일), 5.0mM (6일) 이상의 고농도에서 증가하였다.

• 동의생리병리학회지
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• 제18권6호
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• pp.1821-1824
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• 2004

In order to investigate the effects of Radix Achyranthis Bidentatae (RAB) on the growth and differentiation of human osteoblast-like cells, we supplemented the culture medium of MG-63 cells with various concentrations of RAB water extracts. RAB extracts significantly stimulated cell growth, as confirmed by the colorimetric MTT (3-dimethylthiazol-2,5-diphenyltetrazolium bromide) assay. RAB extracts also increased the alkaline phosphatase (ALP) activity, which is a osteoblast differentiation marker. These results suggest that RAB can stimulate osteoblastic activity and may represent new pharmacological tools for the treatment of osteoporosis.

• 약학회지
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• 제58권5호
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• pp.307-313
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• 2014

Rumex crispus (curled dock), which is a perennial wild plant, has long been used as a laxative, astringent, and medicine to treat blood and skin diseases. We recently reported that the roots of R. crispus are an effective nutraceutical for bone. This study prepared ethanol extracts of the leaves and roots of R. crispus, and analyzed the major constituents using liquid chromatography and mass spectrometry. In addition, their effects on the proliferation and differentiation of human osteoblast-like MG-63 cells, such as cell viability, alkaline phosphatase (ALP) activity, collagen content, and mineralization, were compared. The chromatograms of the chemical constituents of the two extracts exhibited quite different profiles: quercetin and quercitrin were identified as major peaks in the leaf extract, whereas cinnamtannin B1 and procyanidin isomers were the major peaks for the root extract. Neither extract was cytotoxic at concentrations of < $25{\mu}g/ml$. ALP activity and collagen synthesis-which are markers of the early stage of osteogenesis-in MG-63 cells were significantly increased upon the addition of the root extract compared with the addition of the leaf extract. In contrast, the leaf extract had a more stimulatory effect on mineralization-which is marker of the late stage of osteogenesis-in MG-63 cells than did the root extract. In conclusion, extracts of both leaves and roots of R. crispus stimulated the bone-forming activity of osteoblasts; in particular, the root extract was more effective in the early stage of osteoblast differentiation, while the leaf extract was more effective in the late stage. This difference in anabolic activity may be due to differences in the constituents of the leaves and roots. The leaves and roots of R. crispus appear to complement each other as stimulators of bone formation.