• Parasites, Hosts and Diseases
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• v.37 no.1
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• pp.39-46
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• 1999

As a preliminary study for the explanation of pathobiology of Neodiplostomum seoulense infection. a 54 kDa protease was purified from the crude extract of adult worms by sequential chromatographic methods. The crude extract was subjected to DEAE-Sepharose Fast Flow column, and protein was eluted using 25 mM Tris-HC1 (pH 7.4) containing 0.05. 0.1, 0.2 and 0.4 M NaC1 in stepwise elution. The 0.2 M NaCl fraction was further purified by Q-Sepharose chromatography and protein was eluted using 20 mM sodium acetate (pH 6.4) containing 0.05, 0.1. 0.2 and 0.3 M NaCl, respectively. The 0.1M NaCl fraction showed a single protein band on SDS-PAGE carried out on a 7.5-15% gradient gel. The proteolytic activities of the purified enzyme were specifically inhibited by L-trans-epoxy-succinylleucylamide (4-guanidino) butane (E-64) and iodoacetic acid. The enzyme, cysteine protease. showed the maximum proteolytic activity at pH 6.0 in 0.1 M buffer, and degraded extracellular matrix proteins such as collagen and fibronectin with different activities. It is suggested that the cysteine protease may playa role in the nutrient uptake of N. seoulense from the host intestine.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.8
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• pp.61-70
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• 2009

A 10b 30MS/s pipelined ADC operating under 1V power supply is presented. It utilizes a switched-RC based input sampling circuit and a resistive loop to reset the feedback capacitor in the multiplying digital-to-analog converter (MDAC) for the low-voltage operation. Cascaded switched-RC branches are used to achieve accurate grain of the MDAC for the first stage and separate switched-RC circuits are used in the sub-ADC to suppress the switching noise coupling to the MDAC input The measured differential and integral non-linearities of the prototype ADC fabricated in a 0.13${\mu}m$, CMOS process are less than 0.54LSB and 1.75LSB, respectively. The prototype ADC achieves 54.1dB SNDR and 70.4dB SFDR with 1V supply and 30MHz sampling frequency while consuming 17mW power.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2008.10a
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• pp.54-54
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• 2008

• Bulletin of the Korean Space Science Society
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• 1994.09a
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• pp.54-54
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• 1994

No Abstract. See Full-text

• Kyungpook Mathematical Journal
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• v.54 no.4
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• pp.577-586
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• 2014

In this paper, we introduce two new types of irresolute functions namely completely D-irresolute functions and weakly D-irresolute functions. We obtain their characterizations and their basic properties.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2005.11a
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• pp.54-54
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• 2005

• Proceedings of the Materials Research Society of Korea Conference
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• 1998.08a
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• pp.54.2-54
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• 1998

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.54.1-54.1
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• 2008

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.54.2-54.2
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• 2008

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.2
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• pp.103-118
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• 2012

In this study, the cellular protective effect of isoquercitrin against $H_2O_2$ and rose bengal-indued HaCaT cell damage was investigated. The ethosome and elastic liposome for enhanced transdermal delivery were prepared. Particle size, loading efficiency and cumulative permeated amounts of them were evaluated. Isoquercitrin didn't show any characteristic cytotoxicity at 50 ${\mu}M$. When HaCaT cells were treated with 5 mM $H_2O_2$ and 25 ${\mu}M$ rose bengal, isoquercitrin protected the cells against the oxidative damage in a concentration dependent manner (6.25 ~ 50 ${\mu}M$). The size of 0.03 % isoquercitrin loaded ethosome was 222.85 nm and the loading efficiency was 82.26 %. The ethosome loaded with 0.03 % isoquercitrin was stable and maintained the constant particle size for 2 weeks after being prepared. The ethosome exhibited more enhanced skin permeability than general liposome and ethanol solution. The optimal ratio of lipid to surfactant of 0.1 % isoquercitrin loaded elastic liposomes was observed to be 89 : 5 through evaluating particle size (341.2 nm), deformability index (59.89), loading efficiency (54.3 %), and skin permeability (54.4 %).