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Effect of aeration for the probiotic feed production from food wastes by Lactobacillus acidophilus and Saccharomyces cerevisiae (Lactobacillus acidophilus와 Saccharomyces cerevisiae를 이용한 남은 음식물의 생균 사료화에 대한 공기주입의 영향)

  • Lee, Kyung-seok;Lee, Ki-Young;Oh, Chang-seok;Lee, Dae-Gyu;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.11 no.4
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    • pp.114-119
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    • 2003
  • The fermentative conversion of food wastes into probiotic feed was investigated by seeding of mixed inoculum of Lactobacillus acidophilus and Saccharomyces cerevisiae. After grinding finely, optimal fermentation conditions for aeration was investigated at $30^{\circ}C$, The viable cell count of lactic acid bacteria and yeast during fermentation were monitored by controlling aeration rate at each different aeration degree of 0v.v.m 0rpm, 0.25v.v.m 100rpm, 0.5v.v.m 200rpm, and 1v.v.m 500rpm respectively. The most active growth of the yeast was shown at 0.5v.v.m 200rpm as $4.5{\times}10^9CFU/m{\ell}$. By controlling aeration rate, the pH of the probiotics feed could be controlled between 4-5 for the enhancement of preservation characteristics and acceptability for cattle feeding.

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EFFECT OF LIGHT IRRADIATION MODES ON THE MARGINAL LEAKAGE OF COMPOSITE RESIN RESTORATION (광조사 방식이 복합레진 수복물의 변연누출에 미치는 영향)

  • 박은숙;김기옥;김성교
    • Restorative Dentistry and Endodontics
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    • v.26 no.4
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    • pp.263-272
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    • 2001
  • The aim of this study was to investigate the influence of four different light curing modes on the marginal leakage of Class V composite resin restoration. Eighty extracted human premolars were used. Wedge-shaped class Y cavities were prepared on the buccal surface of the tooth with high-speed diamond bur without bevel. The cavities were positioned half of the cavity above and half beyond the cemento-enamel junction. The depth, height, and width of the cavity were 2 mm, 3 mm and 2 mm respectively. The specimens were divided into 4 groups of 20 teeth each. All the specimen cavities were treated with Prime & Bond$^{R}$ NT dental adhesive system (Dentsply DeTrey GmbH, Germany) according to the manufacturer's instructions and cured for 10 seconds except group VI which were cured for 3 seconds. All the cavities were restored with resin composite Spectrum$^{TM}$ TPH A2 (Dentsply DeTrey GmbH, Germany) in a bulk. Resin composites were light-cured under 4 different modes. A regular intensity group (600 mW/${cm}^2$, group I) was irradiated for 30 s, a low intensity group (300 mW/${cm}^2$, group II) for 60 s and a ultra-high intensity group (1930 mW/${cm}^2$, group IV) for 3 s. A pulse-delay group (group III) was irradiated with 400 mW/${cm}^2$ for 2 s followed by 800 mW/${cm}^2$ for 10 s after 5 minutes delay. The Spectrum$^{TM}$ 800 (Dentsply DeTrey GmbH, Germany) light-curing units were used for groups I, II and III and Apollo 95E (DMD, U.S.A.) was used for group IV. The composite resin specimens were finished and polished immediately after light curing except group III which were finished and polished during delaying time. Specimens were stored in a physiologic saline solution at 37$^{\circ}C$ for 24 hours. After thermocycling (500$\times$, 5-55$^{\circ}C$), all teeth were covered with nail varnish up to 0.5 mm from the margins of the restorations, immersed in 37$^{\circ}C$, 2% methylene blue solution for 24 hours, and rinsed with tap water for 24 hours. After embedding in clear resin, the specimens were sectioned with a water-cooled diamond saw (Isomet$^{TM}$, Buehler Co., Lake Bluff, IL, U.S.A.) along the longitudinal axis of the tooth so as to pass the center of the restorations. The cut surfaces were examined under a stereomicroscope (SZ-PT Olympus, Japan) at ${\times}$25 magnification, and the images were captured with a CCD camera (GP-KR222, Panasonic, Japan) and stored in a computer with Studio Grabber program. Dye penetration depth at the restoration/dentin and the restoration/enamel interfaces was measured as a rate of the entire depth of the restoration using a software (Scion image, Scion Corp., U.S.A.) The data were analysed statistically using One-way ANOVA and Tukey's method. The results were as follows : 1. Pulse-Delay group did not show any significant difference in dye penetration rate from other groups at enamel and dentin margins (p>0.05) 2. At dentin margin, ultra-high intensity group showed significantly higher dye penetration rate than both regular intensity group and low intensity group (p<0.05). 3. At enamel margin, there were no statistically significant difference among four groups (p>0.05). 4. Dentin margin showed significantly higher dye penetration rate than enamel margin in all groups (p<0.05).

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Antioxidant Activities of Citrus unshiu Extracts obtained from Different Solvents (추출용매에 따른 진피 추출물의 항산화 활성)

  • Lee, Sung-Gu;Oh, Sung-Cheon;Jang, Jae-Seon
    • The Korean Journal of Food And Nutrition
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    • v.28 no.3
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    • pp.458-464
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    • 2015
  • In this study, the total polyphenol content, electron donating ability (EDA) and inhibitory activity of glutathione S-transferase (GST) of freeze-dried Citrus unshiu extracts were examined. The Citrus unshiu extracts was obtained from four solvents such as ethyl acetate, acetone, methyl chloride and methanol, to evaluate its functional properties. Total polyphenol contents were measured in the two different extracts, and the extracts were screened for their potential antioxidant activities using tests such as electron donating ability (EDA), glutathione S-transferase (GST). The total polyphenol contents of Citrus unshiu extracts were $928.48{\pm}1.19{\mu}g\;GAE/mL$ in ethyl acetate (EA), $886.03{\pm}0.44{\mu}g\;RE/mL$ in acetone (AC), $413.08{\pm}1.39{\mu}g\;GAE/mL$ in methylene chloride (MC), $12,648.60{\pm}0.56{\mu}g\;GAE/mL$ in methanol (MeOH), respectively. Also, the total polyphenol contents of EtOH Citrus unshiu extracts were $664.64{\pm}0.74{\mu}g\;GAE/mL$ in EA, $702.67{\pm}0.85{\mu}g\;RE/mL$ in AC, $429.64{\pm}0.61{\mu}g\;GAE/mL$ in MC, $16,108{\pm}0.73{\mu}g\;GAE/mL$ in MeOH, respectively. The total polyphenol contents were significantly difference (p<0.05) between the solvents. The electron donating ability of Citrus unshiu extracts were $62.80{\pm}0.36%$ in EA, $97.43{\pm}0.51%$ in AC, $52.20{\pm}0.30%$ in MC, $97.63{\pm}0.46%$ in MeOH, respectively. Also, the electron donating ability of EtOH Citrus unshiu extracts were $51.49{\pm}0.26%$ in EA, $63.17{\pm}0.31%$ in AC, $67.68{\pm}0.55%$ in MC, $96.18{\pm}0.41%$ in MA, respectively. The electron donating ability were significantly difference (p<0.05) between the solvents. The inhibitory activity of glutathione S-transferase in Citrus unshiu extracts were $76.22{\pm}0.65%$ in EA, $31.73{\pm}0.48%$ in MC, $97.48{\pm}0.56%$ in MeOH, respectively. Also, inhibitory activity of glutathione S-transferase in EtOH Citrus unshiu extracts were $75.54{\pm}0.55%$ in EA, $73.53{\pm}0.38%$ in MC, $48.70{\pm}0.46%$ in MeOH, respectively. The inhibitory activity of glutathione S-transferase were significantly difference (p<0.05) between the solvents. These results indicated that the Citrus unshiu extracts is a high-valued food ingredient and the extraction with methanol will be useful as a nutritional source with natural antioxidant activities. Considering high consumer demand beneficial health effects, Citrus unshiu extracts can be utilized to develop functional food health- promoting and natural antioxidant agents.

The Effects of Oral Care on Children by Parents (부모의 아동에 대한 구강관리가 아동의 구강관리행위에 미치는 영향)

  • Kang, Hyun Suk;Kim, Kyung Hee;Chung, Hae Kyung
    • Journal of the Korean Society of School Health
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    • v.13 no.2
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    • pp.319-329
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    • 2000
  • This study suggestes that the improvement of oral care through on investigation of the effects of oral care on children by parents. This investigation was done on Sep. 29 1999 through Oct. 8 examining 376 of elementary, 1grade, 2grade, 3grade students in Kyunggi-Do. The content of the questions was analzed with a t-test and a one-way ANOVA; the relation to the oral care on children by parents with oral care behavior was analyzed by a Pearson's Correlation Coefficient. The results are as follow; 1) Statistically women were higher(F=-2.675, p<0.01). The average of the oral care of children was 2.34, man averaged 2.29, woman are raged 2.32. Statistically women were also higher(F=4.682, p<0.052). 2) The more educatior the mother had higher, the higher the grade. Statistically there was a difference(F=6.756, p<0.01. The oral care of children above Uni graduate(M=2.38), high (M=2:23), middle graduate(M=2.Z3). Statistically, the higher the academic degree, the more optimistic(F=4.114, p<0.05). 3) In the oral care on children by parents, when the conversation with the family was often 2.17, there was little difference(F=7.343, p<0.01). In the oral care of children was 2.45, there was little difference(F=7.343. p<0.01). As a result, the conversation with family affects the behavior. 4) In the oral care of children, if the food that they like was fruits, vegetables, and fresh food, the average was 2.37, very high. There was little difference in other cases(F=-3.179, p<0.05). We can conclude that fresh food affects the oral care of children. 5) In the oral care on children by parents, when the course of knowledge on the oral care was viewed by a grandfather(M=2.02), grandmother(M=2.05), father(M=2.14), mother(M=2.08). There was little difference(F=2.666, p<0.05). 6) In oral care, if the level of income was viewed high level(M=2.42), middle(M=2.34), 10w(M=1.88), there was a little difference(F=3.300, p<0.05). 7) The relation of the oral care on children by parents and the oral care was normal. In other words, the more management by parents, the more management by children.(p<0.01) Therefore in this study, the oral care on children by parents effect the oral care behavior of children. To improve the mouth health of children, the parents's interest in their children must be constant. Also, it is needed to develope an education program where parents and children can be educated.

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Non-traditional Straws: Alternate Feedstuffs for Ruminants

  • Kaushal, S.;Wadhwa, M.;Bakshi, M.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.12
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    • pp.1722-1727
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    • 2006
  • The nutritive value of 4 straws, obtained after thrashing of seeds from fodder crops, was assessed as complete feed for ruminants. Sixteen male Murrah buffaloes (liveweight 365.8${\pm}$19.5 kg), were divided into 4 equal groups and offered ad lib. straw of either Trifolium resupinatum, Trifolium alexandrium, Medicago sativa or Lolium perenne, supplemented with minerals and vitamin A, for 40 days in a completely randomized design. Simultaneously, each straw was offered to 3 rumen fistulated male buffaloes in order to assess the biochemical changes in the rumen. Compared to other straws M. sativa straw had higher (p<0.05) organic matter (OM), crude protein (CP), acid-detergent fiber (ADF) and cellulose content. L .perenne had the highest (p<0.05) hemicellulose and lowest (p<0.05) CP and acid-detergent lignin (ADL) content. T. resupinatum had the lowest concentration of cell wall constituents (CWC). The digestibility of nutrients of T. resupinatum and L. perenne straw was similar, but higher (p<0.05) than that of other straws. M.sativa straw showed highest (p<0.05) digestibility of CP. The highest OM digestibility of T. resupinatum and CP digestibility of M. sativa were responsible for highest (p<0.05) total volatile fatty acids and trichloroacetic acid precipitable nitrogen in the strained rumen liquor. The digestible crude protein (DCP) was highest (p<0.05) in M. sativa followed by that in T. alexandrium. The total purine derivatives excreted in urine varied from 0.22-0.32 mmol/kg $W^{.75}/d$. The efficiency of microbial protein synthesis indicated that OM of straws of M. sativa and that of T. alexandrium was used more (p<0.05) efficiently. The microbial protein synthesized was highest in T. resupinatum, but statistically similar to other groups. The values for N-retention and apparent biological value were highest for L. perenne, though comparable with that of M. sativa and T. alexandrium. The available metabolizable energy (ME) was highest (p<0.05) in T. resupinatum followed by that in L. perenne and lowest in M. sativa. It was concluded that all the straws, supplemented with minerals and vitamin A, could be fed exclusively to adult ruminants with no adverse affect, as animals were able to maintain body weight (372${\pm}$20.1 kg).

ON ERDŐS CHAINS IN THE PLANE

  • Passant, Jonathan
    • Bulletin of the Korean Mathematical Society
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    • v.58 no.5
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    • pp.1279-1300
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    • 2021
  • Let P be a finite point set in ℝ2 with the set of distance n-chains defined as ∆n(P) = {(|p1 - p2|, |p2 - p3|, …, |pn - pn+1|) : pi ∈ P}. We show that for 2 ⩽ n = O|P|(1) we have ${\mid}{\Delta}_n(P){\mid}{\gtrsim}{\frac{{\mid}P{\mid}^n}{{\log}^{\frac{13}{2}(n-1)}{\mid}P{\mid}}}$. Our argument uses the energy construction of Elekes and a general version of Rudnev's rich-line bound implicit in [28], which allows one to iterate efficiently on intersecting nested subsets of Guth-Katz lines. Let G is a simple connected graph on m = O(1) vertices with m ⩾ 2. Define the graph-distance set ∆G(P) as ∆G(P) = {(|pi - pj|){i,j}∈E(G) : pi, pj ∈ P}. Combining with results of Guth and Katz [17] and Rudnev [28] with the above, if G has a Hamiltonian path we have ${\mid}{\Delta}_G(P){\mid}{\gtrsim}{\frac{{\mid}P{\mid}^{m-1}}{\text{polylog}{\mid}P{\mid}}}$.

An Optimal P$_{\lambda}^{M}$-Service Policy for an M/G/1 Queueing System

  • Bae, Jong-Ho;Kim, Jong-Woo;Lee, Eui-Yong
    • Proceedings of the Korean Statistical Society Conference
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    • 2003.10a
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    • pp.189-194
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    • 2003
  • We consider an M/G/1 queueing system under P$_{\lambda}^{M}$-service policy. As soon as the workload exceeds threshold ${\lambda}$ > 0, the service rate is increased from 1 to M ${\geq}$ 1 and is kept until the system becomes empty. After assigning several costs, we show that there exists a unique M minimizing the long-run average cost per unit time.

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Continuous dialysis of selected salts of sulphuric acid

  • Bendova, Helena;Snejdrla, Pavel;Palaty, Zdenek
    • Membrane and Water Treatment
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    • v.1 no.3
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    • pp.171-179
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    • 2010
  • The transport of selected salts of sulphuric acid (cobalt, copper, iron(II), manganese, nickel and zinc sulphate) through an anion-exchange membrane Neosepta-AFN was investigated in a counter-current continuous dialyzer at various salt concentrations and volumetric liquid flow rates. The basic transport characteristics - the rejection coefficient of salt and the permeability of the membrane - were calculated from measurements at steady state. The salt concentration in model mixtures was changed in the limits from 0.1 to 1.0 kmol $m^{-3}$ and the volumetric liquid flow rate of the inlet streams was in the limits from $8{\times}10^{-9}$ to $24{\times}10^{-9}m^3\;s^{-1}$. Under the experimental conditions given, the rejection coefficient of salts tested was in the range from 65% to 94%. The lowest values were obtained for iron(II) sulphate, while the highest for copper sulphate. The maximum rejection of salt was reached at the highest volumetric liquid flow rate and the highest salt concentration in the feed. The permeability ($P_A$) of the Neosepta-AFN membrane for the individual salts was in the range from $0.49{\times}10^{-7}m\;s^{-1}$ to $1.8{\times}10^{-7}m\;s^{-1}$ and it can be described by the following series: $P_{FeSO_4}$ < $P_{NiSO_4}$ < $P_{ZnSO_4}$ < $P_{CoSO_4}$ < $P_{MnSO_4}$ < $P_{CuSO_4}$. The permeability of the membrane was strongly affected by the salt concentration in the feed - it decreased with an increasing salt concentration.

Consideration for Setting Reference Range for Adrenocorticotropic Hormone Test according to Blood Collection Time (채혈 시간에 따른 부신피질 자극 호르몬 검사의 참고치 설정에 관한 고찰)

  • Ji-Hye Park;Jin-Ju Choi;Soo-Yeon Lim;Seon-Hee Yoo;Sun-Ho Lee
    • The Korean Journal of Nuclear Medicine Technology
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    • v.27 no.1
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    • pp.42-46
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    • 2023
  • Purpose The reference range described in Adrenocorticotropic Hormone reagent used in our laboratory is 10-60 pg/mL at 8 a.m. to 10 a.m., and 6-30 pg/mL at 8 p.m. to 10 p.m. However, in the case of outpatients, blood is mainly collected between 10 a.m. and 6 p.m., accounting for 57.8% of the total. Therefore, This study is intended to help make a more accurate diagnosis by reevaluating the reference range provided by the manufacturer of the Adrenocorticotropic Hormone reagent and setting split-timed reference range. Materials and Methods The patients collected blood before 10 a.m. were group A (68 people), and the patients collected blood after 10 a.m. were set to group B (80 people). A T-test was performed between groups to test their significance. And it was confirmed whether it was necessary to set the gender classification as a subgroup. The method of setting the reference range was calculated by the Bayesian's method and the Hoffmann's method. Results The reference range of Group A was 8.6 to 60.6 pg/mL by the Bayesian's method, and the Hoffmann's method was 3.6 to 61.3 pg/mL. The reference range of Group B was 6.9 to 50.5 pg/mL when applying the Bayesian's method, and the Hoffmann method's was 2.3 to 48.9 pg/mL. Conclusion This study was concluded that it was necessary to set the split-timed reference range. Through this study, the later the blood collection time, the lower the level of Adrenocorticotropic Hormone, indicating that blood collection time is important for patients with clinical significance. If a large number of subjects are selected and supplemented in the future, it is believed that systematic and accurate reference range can be set.

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