• Title/Summary/Keyword: M. sinensis

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Distribution of medically important freshwater snails and larval trematodes from Parafossarulus manchouricus and Semisulcospira libertina around the Jinyang Lake in Kyongsang-Nam-Do, Korea (경상남도 남강유역의 의용패류 분포 및 흡충류 유충 감염실태 조사)

  • 조해창;정평림이근태
    • Parasites, Hosts and Diseases
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    • v.21 no.2
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    • pp.193-204
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    • 1983
  • The Jinyang Lake is dammed up Nam River, and surrounded by Jinju city and four counties; Jinyang, Sanchong, Hading, and Sachon in kyongsang-Nam-Bo, Korea. The areas around this man-made lake have been known as an endemic focus of clonorchiasis in Korea. The present study was first aimed to know the distribution of freshwater mollusks including medically important snails, and larval trematodes shed from Parafossarulus manchouricus and Semisulcospira libertina. In addition to above studies, water analyses in each snail habitat were carried out in order to figure out a part of their environmental factors. This malaco-ecological survey was done at the sirs areas around upper, middle and lower parts of the lake for 4 months, August-November, 1983. Total nine species of freshwater mollusks were collected throughout the study: 4 species of gastropods: Semisulcospira libertina, Cipangopaludina chinensis, Parafossarulus manchouricus and Radin auricularia, and 5 species of bit.alves; Unio dcuglasiae, Ancdonta woodiana, Lamprotula gottschei, Corbicula yuminea and Limncperma lucustris. Out of nine species of freshwater mollusks, three species of gastropods; S. Zibertina, p. manchouricus and R. auricularia were medically important in terms of the transmission of digenetic trematodes to humans. P. manchouricus and R. auricularia were mainly collected from the shallow ponds and the irrigation channels with the muddy basin, but S. libertine and the bivalves were only collected from the stream of Nam River where the gravels and rocks were dominant. The levels of dissolved oxygen (D.O.) and biochemical oxygen demand \(B.O.D._5)\ of the water specimens sampled from the study areas ranged from 6.0 to 9.6 ppm and from 0.4 to 1.6 ppm respectively. As a result, it is considered that water system around the Jinyang Lake might be relatively clean without any heavy pollution of aquatic microorganisms and organic materials during the period of this study. On the other hand, eight metalic constituents from the water samples were also assayed, and all metalic ions detected were remarkably low below the legal criteria. However, calcium ion in the water samples from the habitats of P. manchouricus was considerably higher than others. Infection rates of digenetic trematodes in the snails were 6.9% in P. manchouricus and 4.8% in S. libertine, respectively. P. manchouricus snails harboring with thc cercariae of Clonorchis sinensis were only 0.14% among the snails examined, and other trematode cercariae except cercaria of C. sinensis were: furcocercus cercariae, cercaria of Loxogenes liberum type I and II. S. libertine snails parasitized with the cercariae of Metagonimus yokogawai were 1, 5% out of the snails examined, and no cercaria of Paragonimus westermani was found in S. libertine snails in the present study. Digenetic trematode cercariae other than M. yokogawai observed in S. libertina snails were: Cercaria yoshidae (B type) , Cercaria cristata, Cercaria innominatum, Cercaria of Centrocestus formosanus and Cercaria nipponensis.

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Predicting the Changes in Cultivation Areas of Walnut Trees (Juglans sinensis) in Korea Due to Climate Change Impacts (기후변화 영향에 따른 호두나무 재배지역 변화 예측)

  • Lee, Sang-Hyuk;Lee, Peter Sang-Hoon;Lee, Sol Ae;Ji, Seung-Yong;Choi, Jaeyong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.4
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    • pp.399-410
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    • 2015
  • The objective of our study was to predict future cultivation areas for walnut trees (Juglans sinensis), using the cultivation suitability map provided from Korea Forest Service and MaxEnt modelling under future climate conditions. The climate conditions in 2050s and 2070s were computed using the Regional Climate Prediction (RCP) 4.5 and 8.5 scenarios with the HadGEM2-AO model. As a result, compared to the present area, the cultivation area of the western Korea including Chungcheongnamdo, Jeollabuk-do, Jeollanam-do decreased on a national scale under RCP 4.5, and those of Gyeongsangbukdo and part of Gyeongsangnam-do decreased under RCP 8.5. However, Gangwon-do which is located in higher altitude over 600 meters than other regions showed increases in cultivation areas of 18.3% under RCP 4.5 and of 56.6% under RCP 8.5 by 2070s. The predicted map showed large regional variations in the cultivation areas with climate change. From the analysis of current top ranking areas, the cultivation areas in Gimcheon-si and Yeongdong-gun dramatically decreased by 2070s under RCP 4.5 and 8.5; that of Gongju-si decreased more under RCP 4.5; and those of Muju-gun and Cheonan-si sustained the areas by 2070s under both scenarios. The results from this study can be helpful for providing a guide for minimizing the loss of walnut production and proactively improving productivity and quality of walnuts with regard to unavoidable climate change in South Korea.

Development of Analytical Method for Determination of Baicalin, Eleutheroside E, and Ligustilide by LC-MS/MS in Raw Materials of Health Functional Foods (LC-MS/MS를 이용한 건강기능식품 원료 중 바이칼린, 엘레우테로사이드 E, 리구스틸라이드 동시분석법 개발)

  • Keum, Eun Hee;Chung, So Young;Lee, Jin Hee;Kim, Meehye
    • Journal of Food Hygiene and Safety
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    • v.32 no.3
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    • pp.193-198
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    • 2017
  • Scutellaria baicalensis, Eleutherococcus senticosus, and Angelica sinensis have been used as raw materials for health functional foods. This study was conducted to develop a novel method to analyze levels of baicalin (Scutellaria baicalensis), eleutheroside E (Eleutherococcus senticosus), and ligustilide (Angelica sinensis) simultaneously in health functional foods. The methanol extracted samples were analyzed and quantified via liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the positive ion mode and the negative ion mode using multiple reaction monitoring. Standard calibration curves confirmed linearity with the correlation coefficient ($r^2$) of > 0.99 at $100-2000{\mu}g/mL$ concentration range. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of $13.0{\sim}35.2{\mu}g/L$ and $39.3{\sim}106.7{\mu}g/L$, respectively. The recovery results ranged between 91.4~109.9% at 3 different concentration levels with relative standard deviations (RSDs) less than 5%. The proposed analytical method was characterized with high accuracy and acceptable precision. The new method would be an effective tool to analyze baicalin, eleutheroside E, and ligustilide simultaneously in raw materials of health functional foods.

Late season commercial mosquito trap and host seeking activity evaluation against mosquitoes in a malarious area of the Republic of Korea

  • Buekett, Douglas-A.;Lee, Won-Ja;Lee, Kwan-Woo;Kim, Heung-Chul;Lee, Hee-Il;Lee, Jong-Soo;Shin, E-Hyun;Wirtz, Robert-A.;Cho, Hae-Wol;Ckaborn, David-M.;Coleman, Russel-E.;Kim, Wan-Y;Klein, Terry-A.
    • Parasites, Hosts and Diseases
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    • v.40 no.1
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    • pp.45-54
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    • 2002
  • Field trials evaluating selected commercially available mosquito traps variously baited with light, carbon dioxide, and/or octenol were conducted from 18-27 September 2000 in a malarious area near Paekyeon-ri (Tongil-Chon) and Camp Greaves in Paju County, Kyonggi Province, Republic of Korea. The host-seeking activity for common mosquito species, including the primary vector of Japanese encephalitis, Culex tritaeniorhynchus Giles. was determined using hourly aspirator collections from a human and propane lantern-baited Shannon trap doting hours when temperatures exceeded $15^{\circ}C$. The total number of mosquitoes and number of each species captured during the test was compared using a block design. Significant differences were observed for the total number of mosquitoes collected, such that, the Mosquito MagnetTM with octenol > Shannon trap > ABC light trap with light and dry ice > Miniature Black Light trap (manufactured by John W. Hock) $\geq$ New Jersey Trap > ABC light trap with light only. Significant differences in numbers collected among trapes were noted for several species including: Aedes vexans (Meigen), Anopheles lesteri Baisas and Hu. An. sinensis Weidemann, An. sineroides Yamada, An. yatsushiroensis Miyazaki. Culex pipiens pallets Coquillett L., Cx. orientalis Edwards and Cx. tritaeniorhynchus. Host-seeking activity for most common species showed a similar bimodal pattern. Results from these field trap evaluations can significantly enhance current vector and disease surveillance efforts especially for the primary vector of Japanese encephalitis, Cx. tritaeniorhunchus.

Infection status with trematode metacercariae in the fresh-water fish from Chunamchosuchi (pond), Uichang-gun, Kyongsangnam-do, Korea (경남 의창군 주남저수지산 담수어의 흡충류 피낭유충 감염상)

  • 손운목;최연순
    • Parasites, Hosts and Diseases
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    • v.35 no.3
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    • pp.165-170
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    • 1997
  • The present study was performed to analyze the infection status of trematode metacercariae in fishes caught from Chunamchosuchi (pond) located in Uichang-gun. Kyongsangnam-do. A total of 130 freshwater fish of 5 species was collected by a fish net and fish traps from November, 1995 to May, 1996. They were examined under a stereomicroscope after artificial digestion with pepsin-HCI solution. A total of 8 species or metacercaria, i. e. Clonorchis sinensis, Echinochnsnw japonicas, Cvathocotwle orientalis, Diplostomun sp.. Metorchis orientalis. Holostephcnw nipponicw, Exorchis oviformis and unidentified echinostome, was detected from them. The metacercariae of C. sinenesis were found in 8/20 (40.0%) Accnthorhodeus Qsmwsi, 20/20 (100%) Cutter breuiccudc, 31/45 (68.9) Cultriculus eigenmanni and 21/25 (84.0%) Pseunorasborc pronga, and the average number of metacercariae detected in each fish species were 1.9, 31.7. I5.3, and 73.0. From the above results, it was confirmed that fresh-water fishes from Chunamchosuchi (pond) were highly infected with metacercariae of avian trematode, i.e. C. orientolis, H. nipponicus. M. oui,entnlis, E. jcponicw and Diplostonum sp., and 4 species of fish. P. parvc, C. breuiccudn, C. eigenmnnni and A. asmussi, were infected with metacaecariae of C. sinensis.

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Phytosocialogical Study of Weed Vegetation around the Climbing Paths on the Ridge of Deogyusan National Park (국립공원 덕유산 능선부 등산로 주변 잡초군락의 식물사회학적 연구)

  • 안영희;정연택;이성기
    • Korean Journal of Environment and Ecology
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    • v.18 no.2
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    • pp.191-196
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    • 2004
  • Mt. Deogyusan, 1,614m in altitude, is located in the southwest of South Korea. Around Mt. Duckyu, many Korean endemic and rare plants are populated, so it is considered a very important biogeographical area where the temperature zones of northern and southern plants are crossed. Because it is the graceful figure of Mt. Duckyu, it is a common mountain where many tourists visit frequently. Continuous tourist's visit may cause a bad influence on vegetation around the climbing paths. Therefore, weed community around the climbing paths on Mt. Duckyu, where visitors exert a bad influence directly on its community by coming in and out, was surveyed phytosocialogically. Our surveys have been accomplished from July to August, 2003. Weed communities formed around the climbing paths on Mt. Duckyu were divided into several patterns and analysed. They have been divided into 8 communities and 4 subcommunities. Community A: Plantago asiatica community, A-a: Carex bostrychostigma subcommunity, A-b: Artemisia princeps var. orientalis subcommunity, B: Sasa borealis community, B-a: Carex siderosticta subcommunity, B-b: Veratrum parulum subcommunity, C: Rubus crataegifolius community, D: Hosta longipes community, E: Tripterygium regelii community, F: Miscanthus sinensis var. purpurascens community, G: Carex okamotoi community, H: Lespedeza maximowiczii community. The flora surveyed in these communities was constituted of 34 families, 83 genera, 12 varieties, and 92 species. Wild plants such as Plantago asiatica, Artemisia princeps var. orientalis, Rubus crataegifolius, Sasa borealis and Miscanthus sinensis var. purpurascens were mostly light loving plants and higher resistant plants against the stamping pressure.

Tree Growth and Ecosystem Conditions of Docheon Forest (Natural Monument No. 514) in Docheon-ri, Yeongdeok - Focusing on Plant Ecosystems - (천연기념물 제514호 영덕 도천리 도천숲의 생태계 현황 및 수목 생육 특성 - 식물생태계 현황을 중심으로 -)

  • Kim, Dong Wook;Hur, Bog Su;Lee, Seung Joo;Kim, Hyo Jeong
    • Korean Journal of Heritage: History & Science
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    • v.44 no.1
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    • pp.122-137
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    • 2011
  • The purpose of this study was to present the characteristics of tree growth and the status of the ecosystem in Yeongdeok Docheon-ri Docheon forest, which was designated as Natural Monument No. 514. Results showed that most of the soil properties were generally within desirable levels except for some properties. The forest was composed of 503 trees. Major trees included Zelkova serrata, Celtis sinensis, Cornus walteri, Diospyros lotus, Hemiptelea davidii, and Evodia daniellii. In particular, trees such as Zelkova serrata, Celtis sinensis, and Cornus walteri showed D.B.H ranging from 10cm to 70cm. These trees appeared to be major tree species since the beginning of Docheon forest's existence. The wild birds observed from the site were from 18 families, 20 species, and 95 individuals. Also were found 1 mammal species, 1 reptile species, and 1 amphibian species. A dead Aix galericulata (designated Natural Monument No. 327) was found in the forest. The annual tree diameter growth of the sample trees was 2.3mm per year on average. The average age of the overstory trees was approximately 101 years, and the oldest tree was estimated to be 300~400 years old.

The Ichthyofauna and Community Structure of Fish at Wondong Marsh in the Kyeongnam Province, Korea (원동습지의 어류상과 군집구조)

  • Yang, Hong-Jun;Kum, Ji-Don;Lee, Yong-Ho
    • Korean Journal of Ichthyology
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    • v.13 no.4
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    • pp.261-266
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    • 2001
  • The ichthyofauna and the structure of fish community were surveyed from June to August in 2000 at four stations of the Wondong marsh, Yangsan city in Kyungnam province. During the study period, 24 species belonging to eight families were collected. Cyprinid fish occupied 62.5% (15 species) and cobitid and centrarchid fish 8.3% (each 2 species). According to relative abundance in the whole marsh, Z. platypus (23.2%) was dominant species and S. gracilis majimae (14.2%) was subdominant species. M. salmoides (9.6%), C. auratus (8.3%), P. herzi (8.3%), M. yaluensis (6.5%), M. anguillicaudatus (4.0%), L. macrochirus (3.4%), P. parva (2.8%), R. ocellatus (2.5%), C. brevicauda (2.5%), C. sinensis (2.5%) and O. platycephala (2.2%) were common species and C. carpio, A. rhombeus, H. labeo, S. variegatus wakiyae, S. chankaensis tsuchigae, Z. temmincki, O. bidens, S. asotus, C. herzi, R. brunneus, C. argus were rare specieswhich occupied less than 2.0% in relative abundance. Six species of S. variegatus wakiyae, S. chankaensis tsuchigae, S. gracilis majimae, M. yaluensis, C. herzi and O. platycephala in observed species were known as endemic species. By analyzing of fish community in the surveyed area, the species diversity and evenness of the fishes were high but dominance was low. And the similarity of the fish species among the sites was mid-grade as more than 0.55 in index.

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Protective effect of Juglans sinensis Dode extract (JS) on oxidant-induced apoptosis in renal epithelial cells (신세뇨관(腎細尿管) 상피세포(上皮細胞)에서 산화(酸化)로 유발(誘發)된 apoptosis에 대한 호도약침액(胡桃藥鍼液)의 방어효과(防禦效果))

  • Park, In-bum;Ahn, Chang-beohm;Jang, Kyung-jeon;Song, Choon-ho;Yoon, Hyoun-min;Kim, Cheol-hong
    • Journal of Acupuncture Research
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    • v.21 no.3
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    • pp.1-12
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    • 2004
  • Objective: This study was undertaken to evaluate the role of lipid peroxidation in oxidant-induced apoptosis and effect of JS on the apoptosis in opossum kidney (OK) cells, an established renal proximal tubular cells. Methods : Exposure of cells to 0.1mM tBHP for 2hr did not induce apoptosis, but subsequent incubation in normal culture medium for 18hr after tBHP treatment induced apoptotic cell death which is dependent of tBHP concentration. Results : JS decreased tBHP-induced apoptotic cell death in a dose-dependent fashion and at concentrations higher than 0.01 mg/ml completely prevented the apoptosis. tBHP-induced apoptosis was prevented by the lipid soluble antioxidant N,N'-diphenyl-p-phenylenediamine (DPPD) and water-soluble antioxidant Trolox. tBHP increased lipid peroxidation, which was inhibited by JS and DPPD. tBHP-induced DNA damage was prevented by JS and DPPD. Conclusion : These results indicate that tBHP induces apoptosis through a lipid peroxidation-dependent mechanism and JS exerts the protective effect against the apoptosis by preventing peroxidation of membrane lipids.

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Occurrence of Downy Leaf Spot on Juglans regia Caused by Microstroma juglandis in Korea (Microstroma juglandis에 의한 호두나무 흰곰팡이병 발생)

  • Lee, Sang-Hyun;Lee, Seung-Kyu;Park, Ji-Hyun;Cho, Sung-Eun;Shin, Hyeon-Dong
    • Research in Plant Disease
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    • v.17 no.3
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    • pp.386-390
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    • 2011
  • In June 2011, walnuts (Juglans regia) from orchards in Kimcheon and Muan, in southern Korea, were found to exhibit downy mildew-like symptoms of a foliar disease. Whitish polygonal efflorescence was produced on the abaxial surface of affected leaves and discolored light green blotches on the corresponding adaxial surfaces. In the later stage of disease development, diseased tissues collapsed and became necrotic. Based on morphological and cultural characteristics, the causal fungus was identified as Microstroma juglandis. The sequence of ITS rDNA of the present isolate showed 100% similarity with those of M. juglandis obtained from GenBank databases, thus confirming its identity. Pathogenicity tests were conducted on leaves of walnut seedlings, fulfilling Koch's postulates. The disease has been previously reported in North America, Europe, Oceania and some western Asia. This is the first report of downy leaf spot on walnuts in East Asia.