• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.989-998
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• 2016

Acidic endo-polygalacturonases are the major part of pectinase preparations and extensively applied in the clarification of fruits juice, vegetables extracts, and wines. However, most of the reported fungal endo-polygalacturonases are active and stable under narrow pH range and low temperatures. In this study, an acidic endo-polygalacturonase (EPG4) was purified and characterized from a mutant strain of Penicillium oxalicum. The N-terminal amino acid sequence of EPG4 (ATTCTFSGSNGAASASKSQT) was different from those of reported endo-polygalacturonases. EPG4 displayed optimal pH and temperature at 5.0 and 60-70℃ towards polygalacturonic acid (PGA), respectively, and was notably stable at pH 2.2-7.0. When tested against pectins, EPG4 showed enzyme activity over a broad acidic pH range (>15.0% activity at pH 2.2-6.0 towards citrus pectin; and >26.6% activity at pH 2.2-7.0 towards apple pectin). The K_m and V_max values were determined as 1.27 mg/ml and 5,504.6 U/mg, respectively. The enzyme hydrolyzed PGA in endo-manner, releasing oligo-galacturonates from PGA, as determined by TLC. Addition of EPG4 (3.6 U/ml) significantly reduced the viscosity (by 42.4%) and increased the light transmittance (by 29.5%) of the papaya pulp, and increased the recovery (by 24.4%) of the papaya extraction. All of these properties make the enzyme a potential application in the beverage industry.

• Journal of Applied Biological Chemistry
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• v.55 no.2
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• pp.129-133
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• 2012

To exploit plant growth promoting bacteria in the roots of Miscanthus sacchariflorus, a biomass energy crop, total 64 bacteria were isolated. For the investigation of plant growth promoting effects from the isolated bacteria, production of indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activities were tested and other cultural conditions were examined. As results, 8 isolates showed plant growth promoting effects on the M. sacchariflorus and an isolate designated Agrobacterium sp. BE516 has the highest activity by enhancing the shoot elongation over 2-fold than the control. Agrobacterium sp. BE516 produced 64 ${\mu}g$ IAA per mL and showed ACC deaminase activity which is involved in the resistance to environmental stress such as high salt and drought. It could grow at low temperature in the range from 4 to $15^{\circ}C$, at pH 4.0 and at 4% NaCl. These results indicate that the Agrobacterium sp. BE516 can be useful as a bio-fertilizer for M. sacchariflorus under the stressed conditions.

• Food Science and Preservation
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• v.12 no.4
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• pp.329-335
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• 2005

Storage quality characteristics of low fat salad dressing with spirulina($0.28\%$) was evaluated. After 2 wks of storage, viscosity decreased according to the prolonged storage time. After 8 wks storage, emulsion stability decreased to $30\%$, which was $25\%$ of freshly made dressing. The fat globule size distribution was not different from that of control until one month of storage, but after 75 days of storage, the fat globule size distribution pattern changed into the increase of larger size($15{\sim}2.0\;{\mu}m$: $11.4\%$ for control, $30.1-32.3\%$ for 75 days of storage). Hunter color of L value decreased, whereas a and b value increased according to the prolonged storage time. TBARS value at 8 wks of storage was increased upto $10\%$ for storage at $5^{\circ}C$ and $15\%$ for storage at $10^{\circ}C$. Antioxidant activity of salad dressing decreased according to the storage temperature and time: $IC_{50}$ values of DPPH radical scavenging activity of 8 wk storage was 157.4 mg/mL at $5^{\circ}C$ and 194.6 mg/mL at $10^{\circ}C$. Total microbial number of salad dressing was increase to 7.9 log(CFU/mL), but E. coli was not detected Based on present condition, low temperature storage was favorable for better quality of spirulina salad dressing.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.6 no.4
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• pp.21-28
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• 1986

Conversion of anaerobic mesophilic digestion to thermophilic range has been investigated using a synthetic sludge. When temperature was raised at a rate of 2, 1, and $0.5^{\circ}C$ per day with continuous feeding, a lower reaction rate was observed with a high rate of temperature change. Although methane fermentation ceased completely for an digesters at thermophilic temperature, acid fermentation continued. Methane fermentation was never achieved even with neutralization during 6 months of resting. The methane formers were completely inactivated by the temperature shock and accumulation of volatile acids due to continuous feeding, while the acid formers lost biological activity quickly, but gradually acclimated to a high temperature. When temperature was raised without feeding, successful thermophilic digestion was achieved with 1 day of resting at thermophilic temperature at a rate of $1^{\circ}C$ per day, and also achieved with 20 days of resting at a direct increase. Conversion to a thermophilic range is easily achieved with resting. A short period of resting is required at a low rate of temperature increase, while a long period of resting enough to balance methane formers with acid fermers makes a conversion possile when temperature is raised at a high rate. Soured thermophilic digesters were recovered after seeding of mesophilic sludges, and sludge seeding could be a good method of start-up, conversion, or recovery of a thermophilic digester. Significant amount of thermophiles seemed to be present in the mesophilic digesters.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.2
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• pp.153-160
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• 2010

A magnetocardiogram (MCG) is a recording of the biomagnetic signals generated by cardiac electrical activity. Biomagnetic instruments are based on superconducting quantum interference devices (SQUIDs). A liquid cryogenic Dewar flask was used to maintain the superconductors in a superconducting state at a very low temperature (4 K). In this study, the temperature distribution characteristics of the liquid helium in the Dewar flask was investigated. The Dewar flask used in this study has a 30 L liquid helium capacity with a hold time of 5 d. The Dewar flask has two thermal shields rated at 150 and 40 K. The temperatures measured at the end of the thermal shield and calculated from the computer model were compared. This study attempted to minimize the heat transfer rate of the cryogenic Dewar flask using an optimization method about the geometric variable to find the characteristics for the design geometric variables in terms of the stress distribution of the Dewar flask. For thermal and optimization analysis of the structure, the finite element method code ANSYS 10 was used. The computer model used for the cryogenic Dewar flask was useful to predict the temperature distribution for the area less affected by the thermal radiation.

• Korean journal of applied entomology
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• v.52 no.1
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• pp.29-33
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• 2013

The fecundity of summer generation adults of arrowhead scale, Unaspis yanonensis (Kuwana) (Hemiptera: Diaspididae), was examined at constant temperatures. The total fecundity increased up to $29^{\circ}C$ and declined thereafter. Total fecundity reached a maximum of 254.5 eggs (crawlers) per female at $29^{\circ}C$. Females showed periodic oviposition activity but the pattern differed with temperature. Three or more oviposition cycles were occurred at 25 and $29^{\circ}C$. At the other temperatures, the 2nd oviposition cycle was reduced or obscure. And oviposition cycle after the 2nd cycle was not obvious at low or high temperatures. The fecundity of the 1st cycle was significantly different according to temperature, showing higher fecundity at 25 and $29^{\circ}C$. Fecundity after 1st cycle at 13, 17 and $21^{\circ}C$ were lower than at 25 and $29^{\circ}C$. The sex ratios of hatched nymphs in terms of female : male in 13, 17, 25, 29 and $33^{\circ}C$ were 1 : 0.48, 1 : 4.79, 1 : 2.48, 1 : 1.85 and 1 : 0.56, respectively.

• Journal of the Korean Chemical Society
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• v.20 no.5
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• pp.323-332
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• 1976

The x or $x^0+x'$ values of the nonstoichiometric chemical formula $TiO_{2-x}$ or $Ti_{2-(x^0+x')}$ have been measured by a specially made magnetic quartz microbalance in a temperature range from 600 to $1300^{\circ}C$ under oxygen pressures of $1{\times}10^{-6} to 1 atm. The standard x or $x^0$ value of the rutile is 0.00148. The x values $under_xoxygen$ pressure of 1 atm decrease with temperatures and then the stoichiometric rutile (or x = 0) is formed at $1130^{\circ}C$. The x values varied between 0.00148 and 0.01719 at a temperature range from 600 to $1300^{\circ}C$ under $1{\times}10^{-9}{\sim}1{\times}10^{-2}$ atm oxygen pressures. The enthalpies of formation of the nonstoichiometric rutile, $H_f$, varied between 21.05 and 29.97 Kcal/mole under the above conditions. The 1/n values calculated from the plots of log X' vs. log $Po_2$ are -{\frac{1}{2}}{\sim}-{\frac{1}{4}} under low oxygen pressure range of $1{\times}10^{-6}\;to\;1{\times}10^{-4}$ atm. Many physical properties of the titanium dioxide, such as the stability of the rutile, Electrical conductivity, catalytic activity and defects, can be explained through the x values and the thermodynamic data calculated from the temperature and oxygen pressure dependences of the x' values.

• Clean Technology
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• v.18 no.1
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• pp.95-101
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• 2012

The Co and Ni catalysts supported on $Al_2O_3$ for partial oxidation of methane producing hydrogen were synthesized using impregnation to incipient wetness. And the promotion effects of metals such as Mg, Ce, La and Sr in partial oxidation of methane over these $Co/Al_2O_3$ and $Ni/Al_2O_3$ were investigated. Reaction activity of these catalysts for the partial oxidation of methane was investigated in the temperature range of 450~$650^{\circ}C$ at 1 atm and $CH_2/O_2$ = 2.0. The catalysts were characterized by BET, XRD and SEM/EDX. The results indicated that the catalytic performance of these catalysts was improved with the addition of 0.2 wt% metal promoter. The Mg promoted $Co/Al_2O_3$ catalyst showed the highest $CH_4$ conversion and hydrogen selectivity at higher temperature than $500^{\circ}C$. The Ce and Sr promoted Ni catalysts superior to Co-based catalysts in the low temperature range. The addition of metal promoter to $Co/Al_2O_3$ and $Ni/Al_2O_3$ catalysts increased the surface area.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.3
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• pp.623-628
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• 2009

The emergence of compact and low-power wireless communication sensors and actuators in the technology supporting the ongoing miniaturization of processing and storage allows for entirely the new kinds of embedded systems. These systems are distributed and deployed in environments where they may have been designed into a particular control method, and are often very dynamic. Collection of devices can communicate to achieve a higher level of coordinated behavior. Wireless sensor nodes deposited in various places provide light, temperature, and activity measurements. Wireless sensor nodes attached to circuits or appliances sense the current or control the usage. Together they form a dynamic and multi-hop routing network connecting each node to more powerful networks and processing resources. Wireless sensor networks are a specific-application and therefore they have to involve both software and hardware. They also use protocols that relate to both applications and the wireless network. Wireless sensor networks are consumer devices supporting multimedia applications such as personal digital assistants, network computers, and mobile communication devices. Wireless sensor networks are becoming an important part of industrial and military applications. The characteristics of modem embedded systems are the capable of communicating adapting the different operating environments. In this paper, We designed and implemented sensor network system which shows through host PC sensing temperature and humidity data transmitted for wireless sensor nodes composed wireless temperature and humidity sensor and designs sensor nodes using embedded system with the intention of studying USN.

• Safety and Health at Work
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• v.8 no.4
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• pp.386-392
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• 2017

Background: Fire service workers often implement multiple duties in the emergency conditions, with such duties being mostly conducted in various ambient temperatures. Methods: The aim of the current study was to assess the firefighters' physiological responses, information processing, and working memory prior to and following simulated firefighting activities in three different hot environments. Seventeen healthy male firefighters performed simulated firefighting tasks in three separate conditions, namely (1) low heat (LH; $29-31^{\circ}C$, 55-60% relative humidity), (2) moderate heat (MH; $32-34^{\circ}C$, 55-60% relative humidity), and (3) severe heat (SH; $35-37^{\circ}C$, 55-60% relative humidity). It took about 45-50 minutes for each firefighter to finish all defined firefighting activities and the paced auditory serial addition test (PASAT). Results: At the end of all the three experimental conditions, heart rate (HR) and tympanic temperature (TT) increased, while PASAT scores as a measure of information processing performance decreased relative to baseline. HR and TT were significantly higher at the end of the experiment in the SH ($159.41{\pm}4.25beats/min$; $38.22{\pm}0.10^{\circ}C$) compared with the MH ($156.59{\pm}3.77beats/min$; $38.20{\pm}0.10^{\circ}C$) and LH ($154.24{\pm}4.67beats/min$; $38.17{\pm}0.10^{\circ}C$) conditions (p < 0.05). There was no significant difference in PASAT scores between LH and MH (p > 0.05). Nonetheless, there was a measurable difference in PASAT scores between LH and SH (p < 0.05). Conclusion: These consequences demonstrate that ambient temperature is effective in raising the physiological responses following firefighting activities. It is therefore argued that further increase of ambient temperature can impact firefighters' information processing and working memory during firefighting activity.