• Journal of Ecology and Environment
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• 제43권2호
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• pp.254-262
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• 2019

Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15-25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50-80% of the length of the seeds' development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L. insularis and L. harae germinated within 4 weeks after sowing at 15 ℃ and 20 ℃, respectively. Contrarily, L. praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 ℃, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 ℃, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 ℃). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 ℃) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 ℃, 20 ℃, 15 ℃, and 20 ℃, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.

• Mycobiology
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• 제51권2호
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• pp.67-71
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• 2023

A powdery mildew (Erysiphaceae) has been continuously collected on the leaves of Lonicera harae in the southern part of the Korean Peninsula, where this shrub is indigenous. Microscopic examination of the asexual morphs revealed that the current collections are differentiated from the all known Erysiphe species on Lonicera spp. by its longer conidiophores and longer conidia. Although the morphology of the chasmothecia is reminiscent of Erysiphe ehrenbergii and E. lonicerae, the specimens on L. harae differ from them in having smaller ascospores. A phylogenetic tree generated from a combined dataset of the internal transcribed spacer region and 28S rDNA gene sequences demonstrates that sequences obtained from three powdery mildew collections on L. harae clustered together as an independent species clade with high bootstrap values distant from other Erysiphe species on Lonicera, representing a species of its own. Based on morphological differences and molecular-phylogenetic results, the powdery mildew on L. harae is proposed as a new species, Erysiphe lonicerigena, and the holomorph of the fungus is described and illustrated in this study.

• 생약학회지
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• 제41권2호
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• pp.94-96
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• 2010

Crude drug 'InDong(忍冬)' has been used mainly to cure common cold, high fever and gonorrhea. With regard to the botanical origin of InDong, it has been considered to be Lonicera species of Caprifoliaceae, but there was no pharmacognostical conformation on it. To clarify the botanical origin of 'InDong', the anatomical characteristics of the branches of Lonicera species growing wild in Korea, Lonicera japonica and L. japonica forma. chinensis were studied. As a result, it was clarified that InDong was the branches of Lonicera japonica.

• 한국식품영양학회지
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• 제28권3호
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• pp.343-350
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• 2015

Lonicera japonica 에탄올 추출물의 처리에 의하여 LPS에 의해서 활성화된 RAW 264.7 세포에서 NO의 생성량과 TNF-${\alpha}$, IL-$1{\beta}$ 및 IL-6와 같은 염증성 사이토카인의 분비를 억제하였고, MAPK family인 ERK, p38 및 JNK의 인산화와 $I{\kappa}-B{\alpha}$의 분해를 억제하였다. LPS로 유도한 endotoxin shock 동물실험에서 Lonicera japonica 에탄올 추출물 20 mg/kg에서 LPS로 유도한 endotoxin shock에 대한 생존율을 3배 이상 증가시켰으며, 생존시간도 1.3~1.4배 증가시켰다.

• 한국자원식물학회지
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• 제28권4호
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• pp.411-418
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• 2015

Lonicera caerulea var. edulis is a rare species found in some alpine region of Korea. Genetic variation in L. caerulea var. edulis has been investigated by examining 161 individuals from six natural populations: Mt. Seorak 1, Mt. Seorak 2, Mt. Jeombong, Mt. Bangtae, Mt. Gyebang, Mt. Halla. The mean genetic diversity for all the six populations was 0.25 (S.I.). The highest genetic diversity was found in Mt. Seorak (S.I.=0.3158) and the lowest was in Mt. Gyebang (S.I.=0.1047). Comparatively low level of genetic diversity was observed (Ae=1.25, P= 64.6%, S.I.=0.25), which is a typical pattern for rare tree species. AMOVA showed exceptionally large proportion of genetic variations both for among populations (34.69%) and within populations (65.31%). Excluding Mt. Gyebang, the genetic variation among and within population was 18.71% and 81.29% respectively. The UPGMA dendrogram based on genetic distance is not suitable for geographic relationship. Genetic distance of Mt. Gyebang was most distant from the other populations. Excluding Mt. Gyebang, the genetic identities among the five populations were 0.95 to 0.97, which is very high similarity level of genetic identity. This low level of genetic variations and the lack of site in nature indicates that Lonicera caerulea var. edulis demanded a serious conservation.

• Mycobiology
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• 제50권2호
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• pp.150-154
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• 2022

Rhytisma lonicericola was identified as a tar spot fungus on Lonicera sp. in 1902, and has since been recorded on several species of Lonicera in China, Japan, and Korea. Most of the previous records of R. lonicericola have been based on a list of disease occurrences in the absence of any formal morphological identification or molecular analyses. Using six newly obtained specimens collected in the past 2 years, we confirmed the tar spot fungus found on L. japonica in Korea as R. lonicericola based on morphological examinations and molecular phylogenetic analyses. This fungus was distinguished from R. xylostei, another tar spot fungus on Lonicera, by ascospore size and geographical distributions. We present detailed mycological information and, for the first time, DNA sequence data useful for the identification of R. lonicericola.

• 한국응용곤충학회지
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• 제58권3호
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• pp.203-207
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• 2019

노린재목(Hemiptera) 가루이과(Aleyrodidae)에 속하는 담배가루이(Bemisia tabaci Gennadius)는 전 세계적으로 900종 이상의 다양한 작물에 경제적 피해를 주고 있는 주요 해충이다. 특히 이 중 담배가루이 JpL (Lonicera japonica)은 현재까지 일본과 한국에서만 서식하는 종으로 보고되어 있으며, 주로 인동덩굴(Lonicera japonica) 및 일부 식물 종에만 서식하는 것으로 알려져 있다. 본 연구는 2019년 제주도 지역 담배가루이 JpL의 발생 현황 및 확산 정도를 확인하기 위하여 수행되었으며, 총 25개 채집지역 중 24개 지역의 인동덩굴 및 1개 지역의 큰망초(Conyza sumatrensis)에서 담배가루이 JpL 성충 1,003개체를 채집하였다. 채집된 담배가루이 성충의 유전자 염기서열을 분석한 결과, 분석된 모든 담배가 루이는 JpL로 확인되었으며, 본 연구의 결과 담배가루이 JpL의 개체수는 이전에 국내에서 보고된 이후 현재까지 매우 빠른 속도로 증가하고 있음을 확인하였다.

• 한국환경복원기술학회지
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• 제2권1호
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• pp.72-82
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• 1999

The objective of this study was to covering of vine the traffic noise barrier and analyse of their growth characteristics. For the purpose of the experiments, chosen were 28 plants. In January 1996 to December 1998, the nursery seedbeds on the Chonju Arboretum in Korea Highway Corporation were seeding and cutting with those species, and a few species were carried out Honam Highway field experiments. The results are summarized as follows ; The germination ratio of seedbed experiments were Wisteria floribunda(88%), Lonicera japonica(86%), Parthenocissus tricuspidata, Paederia scandens(85%), Celastrus orbiculatus(76%), Clematis terniflora var. denticulata(52%), Clematis mandshurica(44%) respectively. The rooting ratio of cutting experiments were Trachelospermum asiaticum var. intermedium(88%), Parthenocissus quinquefolia(87%), Trachelospermum asiaticum var. asiatica(85%), Kadsura japonica(82%), Hedera rhombea(81%), Euonymus fortunei var. radicans(83%), Trachelospermum jasminoides var. pubescens(80%), Lonicera japonica cv. Aureo-reticulata(80%), Trachelospermum asiaticum var. majus(78%), Euonymus radicans cv. Aureo-marginata(66%), in descending order. As a conclusion, this study shows that the traffic noise barrier was effected by Paederia scandens, Lonicera japonica, Parthenocissus quinquefolia, Wisteria floribunda, Parthenocissus tricuspidata in the field experiments and the others were necessary to maintainable management.

• 식물분류학회지
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• 제44권3호
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• pp.202-207
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• 2014

섬괴불나무(Lonicera insularis Nakai)는 인동과(Caprifoliaceae) 인동속(Lonicera L.) 에 속하는 관목으로 울릉도와 독도의 해안가 지역에 분포하는 한국특산식물이다. 섬괴불나무와 인동속의 근연종인 괴불나무, 청괴불나무, 각시괴불나무, 길마가지나무, L. morrowii, 병꽃나무 7분류군과의 유연관계를 밝히고, 섬괴불나무의 종내 변이를 확인하기 위해 엽록체 DNA 5개 영역의 염기서열을 분석하였다. 분석결과 전체 3.2kb의 염기서열이 결정되었고, 섬괴불나무와 L. morrowii 에서 2개의 엽록체 DNA haplotype(CP01-02)이 결정되었다. 섬괴불나무는 한 개의 염기치환으로 CP01 type과 CP02 type으로 구별되었으며, 일본의 L. morrowii와는 CP02 type을 공유하였다. 섬괴불나무에서 확인 된 두 개의 CP type은 1) 진화적으로 뚜렷한 두 개 이상의 계통을 가지며, 이는 하나 이상의 유입경로를 통해 울릉도로 유입되었을 가능성을 높게 지지한다. 그리고, 2) 형태학적으로 구별되는 섬괴불나무와 L. morrowii의 CP type의 공유는 두 종의 지리적인 장벽에 의한 이소적종분화의 결과로 추론할 수 있다. 본 연구에서 확인된 울릉도와 독도의 섬괴불나무의 종내 변이 및 다양성에 관한 결과는 울릉도 및 독도 식물의 분자생물지리학적 연구로 대양섬의 생물학적 진화양상과 종 분화 과정에 중요한 기초 자료로 활용될 것이다.

• 한국약용작물학회지
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• 제20권1호
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• pp.20-26
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• 2012

This study compared the differences between the Dendranthema boreale and Dendranthema indicum in their habitat, soil adaptability, species composition and community structure in Korea. More D. boreale distributed than D. indicum to in the place where high elevation and on the surface of low degree slope. Both D. boreale and D. indicum growed well in south-east direction of the slope. The soil pH of D. boreale and D. indicum was 6.1 and 7.1, respectively. Ca, Mg, Na and organic matter content of the soil of D. boreale habitat was significantly lower than that of the D. indicum habitat. There were 102 and 88 taxa, in D. boreale and D. indicum habitat, respectively. Both species generally distributed along with herbs than along with trees. The important species found in D. boreale habitat were Artemisia princeps (57.1%) and Humulus japonicus (33.3%), and the D. indicum habitat were Miscanthus sinensis (42.9%) and Lonicera japonica (38.1%). The D. boreale group was classified into Artemisia princeps, Crepidiastrum denticulatum, Miscanthus sinensis, Humulus japonicus, Pueraria lobata, Lespedeza bicolor, Lonicera japonica and Rubus crataegifolius community. The D. indicum group was classified into Artemisia capillaris, Peucedanum japonicum, Boehmeria pannosa, Pinus thunbergii, Lonicera japonica, Quercus acutissima and Robinia pseudoacacia community. There is a large difference bewteen D. boreale and D. indicum in their habitat, soil adaptability, species composition and community structure.