Journal of Ecology and Environment

The Ecological Society of Korea (한국생태학회)
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Ecophysiology of seed dormancy and germination in four Lonicera (Caprifoliaceae) species native to Korea

  • Park, HyungBin (Division of Plant Resources, Korea National Arboretum) ;
  • Ko, ChungHo (Division of Plant Resources, Korea National Arboretum) ;
  • Lee, SeungYoun (Division of Plant Resources, Korea National Arboretum) ;
  • Kim, SangYong (Division of Plant Resources, Korea National Arboretum) ;
  • Yang, JongCheol (Division of Plant Resources, Korea National Arboretum) ;
  • Lee, KiCheol (Division of Plant Resources, Korea National Arboretum)
  • Received : 2019.04.04
  • Accepted : 2019.05.20
  • Published : 2019.06.30
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Abstract

Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15-25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50-80% of the length of the seeds' development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L. insularis and L. harae germinated within 4 weeks after sowing at 15 ℃ and 20 ℃, respectively. Contrarily, L. praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 ℃, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 ℃, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 ℃). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 ℃) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 ℃, 20 ℃, 15 ℃, and 20 ℃, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.

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References

  1. Adams CA, Baskin JM, Baskin CC. Trait stasis versus adaptation in disjunct relict species: evolutionary changes in seed dormancy-breaking and germination requirements in a subclade of Aristolochia subgenus Siphisia (Piperales). Seed Sci Res. 2005;15:161-73. https://doi.org/10.1079/SSR2005207
  2. Baskin CC, Baskin JM. Seeds: ecology, biogeography, and, evolution of dormancy and germination. San Diego: Academic; 1998.
  3. Baskin CC, Baskin JM. When breaking seed dormancy is a problem try a movealong experiment. Nat Plants J. 2003;4:17-21. https://doi.org/10.3368/npj.4.1.17
  4. Baskin CC, Baskin JM. Germination seeds of wildflowers, an ecological perspective. HortTechnology. 2004a;14:467-73. https://doi.org/10.21273/HORTTECH.14.4.0467
  5. Baskin CC, Meyer SE, Baskin JM. Two types of morphophysiological dormancy in seeds of two genera (Osmorhiza and Erythronium) with an Arcto-Tertiary distribution pattern. Am J Bot. 1995;82:293-8. https://doi.org/10.1002/j.1537-2197.1995.tb12633.x
  6. Baskin JM, Baskin CC. Germination ecophysiology of the woodland herb Osmorhiza longistylis (Umbelliferae). Am J Bot. 1984;71:687-92. https://doi.org/10.1002/j.1537-2197.1984.tb14175.x
  7. Baskin JM, Baskin CC. Nondeep complex morphophysiological dormancy in seeds of Osmorhiza claytonii (Apiaceae). Am J Bot. 1991;78:588-93. https://doi.org/10.1002/j.1537-2197.1991.tb15225.x
  8. Baskin JM, Baskin CC. A classification system for seed dormancy. Seed Sci Res. 2004b;14:1-16. https://doi.org/10.1079/SSR2003150
  9. Baskin JM, Davis BH, Baskin CC, Gleason SM, Cordell S. Physical dormancy in seeds of Dodonaea viscosa (Sapindales, Sapindaceae) from Hawaii. Seed Sci Res. 2004;14:81-90. https://doi.org/10.1079/SSR2003157
  10. De Souza TV, Voltolini CH, Santos M, Paulilo MTS. Water absorption and dormancy-breaking requirements of physically dormant seeds of Schizolobium parahyba (Fabaceae-Caesalpinioideae). Seed Sci Res. 2012;22:169-76. https://doi.org/10.1017/S0960258512000013
  11. Doussi MA, Thanos CA. Ecophysiology of seed germination in Mediterranean geophytes. 1. Muscari spp. Seed Sci Res. 2002;12:193-201. https://doi.org/10.1079/SSR2002111
  12. Finch-Savage WE, Leubner-Metzger G. Seed dormancy and the control of germination. New Phytol. 2006;171:501-23. https://doi.org/10.1111/j.1469-8137.2006.01787.x
  13. Geneve RL. Impact of temperature on seed dormancy. HortScience. 2003;38:336-40. https://doi.org/10.21273/HORTSCI.38.3.336
  14. Hidayati SN, Baskin JM, Baskin CC. Dormancy-breaking and germination requirements for seeds of Diervilla lonicera (Caprifoliaceae), a species with underdeveloped linear embryos. Can J Bot. 2000a;78:1199-205. https://doi.org/10.1139/b00-094
  15. Hidayati SN, Baskin JM, Baskin CC. Dormancy-breaking and germination requirements of seeds of four Lonicera species (Caprifoliaceae) with underdeveloped spatulate embryos. Seed Sci Res. 2000b;10:459-69. https://doi.org/10.1017/S0960258500000507
  16. Jeong KS, Kim MS, Lee W, Park JH. Intraspecific variation and geographic study of Lonicera insularis (Caprifoliaceae) based on chloroplast DNA sequences. Kor J Pl Taxon. 2014;44:202-7. https://doi.org/10.11110/kjpt.2014.44.3.202
  17. Kim SM, Won YH, Jeong K, Kim MJ, Chun WJ, Yang HJ, Kwon YS. Chemical constituents of Lonicera maackii leaves. Kor J Pharmacogn. 2016;47:117-21.
  18. Korea National Arboretum. 2019. http://www.nature.go.kr. Accessed 25 Jan 2019.
  19. Lee TB. Coloured flora of Korea. Hyangmunsa, Seoul; 2003.
  20. Martin AC. The comparative internal morphology of seeds. Am Midl Naturalist. 1946;36:513-660. https://doi.org/10.2307/2421457
  21. Naugzemys D, Zilinskaitė S, Denkovskij J, Patamsyte J, Literskis J, Zvingila D. RAPD based study of genetic variation and relationships among Lonicera germplasm accessions. Biologija. 2007;53:34-9.
  22. Nikolaeva MG. Factors controlling the seed dormancy pattern. In: The physiology and biochemistry of seed dormancy and germination (ed. A. A. Khan). Amsterdam: North-Holland Publishing. 1977. p. 51-74.
  23. Phartyal SS, Kondo T, Baskin JM, Baskin CC. Temperature requirements differ for the two stages of seed dormancy break in Aegopodium podagraria (Apiaceae), a species with deep complex morphophysiological dormancy. Am J Bot. 2009;96:1086-95. https://doi.org/10.3732/ajb.0800379
  24. Ryuk JA, Lee HW, Ko BS. Discrimination of Lonicera japonica and Lonicera confusa using chemical analysis and genetic marker. Kor J Herbology. 2012;27:15-21. https://doi.org/10.6116/kjh.2012.27.6.15
  25. Santiago A, Herranz JM, Copete E, Ferrandis P. Species-specific environmental requirements to break seed dormancy: implications for selection of regeneration niches in three Lonicera (Caprifoliaceae) species. Bot. 2012;91:225-33. https://doi.org/10.1139/cjb-2012-0169
  26. Theis N, Donoghue MJ, Li J. Phylogenetics of the Caprifolieae and Lonicera (Dipsacales) based on nuclear and chloroplast DNA sequences. Syst Bot. 2008;33:776-83. https://doi.org/10.1600/036364408786500163
  27. Walck JL, Hidayati SN, Okagami N. Seed germination ecophysiology of the Asian species Osmorhiza aristata (Apiaceae): comparison with its North American congeners and implications for evolution of types of dormancy. Am J Bot. 2002;89:829-35. https://doi.org/10.3732/ajb.89.5.829
  28. Walck JL, Karlsson LM, Milberg P, Hidayati SN, Kondo T. Seed germination and seedling development ecology in world-wide populations of a circumboreal Tertiary relict. AoB plants. 2012;2012:pls007.
  29. Yuan Y, Song L, Li M, Liu G, Chu Y, Ma L, Zhou Y, Wang X, Gao W, Qin S, Yu J, Wang X, Huang L. Genetic variation and metabolic pathway intricacy govern the active compound content and quality of the Chinese medicinal plant Lonicera japonica thunb. BMC Genomics. 2012;13:195. https://doi.org/10.1186/1471-2164-13-195

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