• Toxicological Research
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• v.18 no.1
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• pp.59-64
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• 2002

Substantial evidences have been accumulated about the hormone-like effects of exogenous substances such as pesticides and industrial chemicals during past years. The effects of these substances on the endocrine system are believed to be either enhancing or reducing of various endocrine action. It is necessary to identify putative causal agents by the batter system and to assess their ability to disrupt the endocrine system. A variety of in vitro and In vivo approaches have been used to determine the androgenic effects of environmental chemicals. To establish the method for assessment of the putative endocrine disruptors with androgenic activity, we carried out the cell proliferation assay by MTS method after treatment with the various concentration of testosterone in LNCaP cells (human prostatic cancer cell line) and also observed the expression of androgen-related genes by quantitative RT-PCR. In the cell proliferation assay, the results showed that the grouth of LNCaP cells increased within level of at least 10pM testosterone. We measured by quantitative RT-PCR method on the effects of testosterone on mRNA expression of androgen receptor (AR), prostate-specific antigen (PSA), bone morphogenetic protein (BMP) and BMP receptor (BMPR) In LNCaP cells. The results demonstrated that mRNA expression of PSA and BMPR-IB was observed differently within level of at least 0.01 pM testosterone compared with non-treated control. These observations suggest that the detection of PSA and BMPR-IB mRNA by the quantitative RT-PCR in LNCaP cells is very sensitive method to identify the endocrine disruptors to have the androgenic effects.

• Korean Journal of Biological Psychiatry
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• v.7 no.1
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• pp.14-20
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• 2000

Recently atypical antipsychotics have been used as first line agent in the treatment of schizophrenia, and also played a significant role in the treatment of many kinds of psychiatric disorders. The pharmacokinetic and pharmacodynamic properties of these newer antipsychotics are well known through preclinical and early clinical trials. However, it is important to note the limitations of the results due to its relatively short experience. Clozapine is eliminated principally by the hepatic P450 1A2 and 3A4 cytochrome enzymes. 1A2 inducers such as carbamazepine and smoking can reduce its half-life, while 1A2 inhibitors such as SSRIs, especially fluvoxamine can increase its duration of action. Carbamazepine should be avoided in a patient on clozapine because of carbamazepine's potential effects on bone marrow. Benzodiazepines tend to increase the chances of sedation, delirium and respiratory depression. Risperidone is metabolized to 9-hydroxyriperidone by the hepatic P450 2D6 cytochrome enzymes. Fluoxetine and paroxetine, 2D6 inhibitors interfere with metabolism, but 9-hydroxyrisperidone has similar biological activity as parental drug, so it has little affect on the outcome. Olanzapine shows minimal capacity to inhibit cytochrome P450 isoenzymes and shows minimal chance of drug interaction. It is eliminated principally by the hepatic P450 1A2 and 2D6 cytochrome enzymes.

• The Korea Journal of Herbology
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• v.32 no.1
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• pp.69-74
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• 2017

Objectives : This study aimed to investigate the effects of Angelicae sinensis Radix on longitudinal bone growth rate in rats. We have screened traditional medicinal herbs to develop the longitudinal bone growth stimulator by well-established rat model. A. sinensis was identified as one of the effective herbs in the screening process. Methods : Adolescent female rats were administered A. sinensis at doses of 30 mg/kg and 300 mg/kg for 10 consecutive days. To observe the rate of longitudinal bone growth, tetracycline was injected intraperitoneally on day 8 to stain a fluorescent band on the anew formed bone. To elucidate the mode of action, we observed insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) expression after A. sinensis administration in growth plate. Results : In the 300 mg/kg A. sinensis group, the length between the proximal endpoint of the tetracycline label and the division line between growth plate and bone was significantly increased compared with vehicle-treated control group. Height of the proximal tibial growth plate was higher in the A. sinensis group compared with control group. A. sinensis also upregulated the expressions of IGF-1 and BMP-2 in the proliferative zone and hypertrophic zone of the proximal tibial growth plate. Conclusions : A. sinensis increases longitudinal bone growth rate in rats. According to immunohistochemistry, A. sinensis increases local IGF-1 and BMP-2 expressions in the growth plate which can be considered as direct stimulation of GH on the local growth plate.

• The Korean Journal of Physiology
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• v.20 no.1
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• pp.125-133
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• 1986

The effect of ethanol on the contraction of intestinal smooth muscle in Na-free solution was studied using cat ileal longitudinal muscle strips. Ethanol $(0.5{\pm}4%)$ inhibited both the spontaneous mechanical activity and base-line tension in normal physiological salt solution. However, in Na-free solution it induced a reversible contraction. The excitatory effect by ethanol in Na-free solution was increased with increasing the concentrations of ethanol and the time incubated in Na-free solution. The excitatory response by ethanol was reduced by increasing the concentrations of Na in incubated medium . Ethanol-induced contractile response was not affected by $Ca^{2+}$ removal in bathing medium. In Na-free solution, the contraction by ethanol was inhibited by Las. but was not affected by verapamil. The contraction induced by Na removal in solution was inhibited by the pretreatment of ethanol. These results suggest that ethanol may induce the contraction by increasing the release of superficially membrane-bound $Ca^{2+}$ and/or intracellular $Ca^{2+}$ in Na-free physiological salt solution.

• Journal of Convergence Korean Medicine
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• v.5 no.1
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• pp.45-54
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• 2023

Objectives : Alcohol-induced liver disease advances as to reactive oxygen species (ROS) and cellular lipid peroxidation increase. We examined the hepatoprotective effects of Zingiber officinale Roscoe rhizome extract (ZR), Pueraria lobata Ohwi flower extracts (PF), and a newly developed herbal juice (HJ), which was a combination of ZR and PF extracts, against ethanol-induced hepatotoxicity. Methods: The study utilized the human hepatoma cell line HepG2 cells to validate the hepatoprotective effect of HJ (50~200 ㎍/mL) against ethanol (EtOH, 700 mM)-induced liver damage. Results: HJ effectively reduced the protein expression of sterol regulatory element-binding transcription factor 1, adiponectin, and AMP-activated protein kinase in EtOH-induced HepG2 cells. The levels of ROS, total cholesterol, and triglycerides, which are the result of various synthesis and lipogenesis processes induced by EtOH in the liver, were reduced by HJ. Furthermore, the activities of alcohol dehydrogenase and aldehyde dehydrogenase, enzymes linked to alcohol degradation, were more effectively downregulated by HJ treatment compared to treatment with ZR and PF alone, all without causing cytotoxic effects. Conclusions: HJ protects the liver by inhibiting EtOH-induced lipogenesis, lowering ROS generation, and improving alcohol degradation, which is more effective than ZR and PF alone. Further, in vivo experiments can offer additional evidence regarding the effectiveness, safety, and underlying mechanism of action of HJ.

• Ocean Systems Engineering
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• v.13 no.4
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• pp.349-365
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• 2023

Targeting a floating wave and offshore wind hybrid power generation system (FWWHybrid) designed in the Republic of Korea, this study examines the impact of the interaction, with multiple wave energy converters (WECs) placed on the platform, on platform motion. To investigate how the motion of WECs affects the behavior of the FWWHybrid platform, it was numerically compared with a scenario involving a 'single-body' system, where multiple WECs are constrained to the platform. In the case of FWWHybrid, because the platform and multiple WECs move in response to waves simultaneously as a 'multi-body' system, hydrodynamic interactions between these entities come into play. Additionally, the power take-off (PTO) mechanism between the platform and individual WECs is introduced for power production. First, the hydrostatic/dynamic coefficients required for numerical analysis were calculated in the frequency domain and then used in the time domain analysis. These simulations are performed using the extended HARP/CHARM3D code developed from previous studies. By conducting regular wave simulations, the response amplitude operator (RAO) for the platform of both single-body and multi-body scenarios was derived and subsequently compared. Next, to ascertain the difference in response in the real sea environment, this study also includes an analysis of irregular waves. As the floating body maintains its position through connection to a catenary mooring line, the impact of the slowly varying wave drift load cannot be disregarded. To assess the influence of the 2^nd-order wave exciting load, irregular wave simulations were conducted, dividing them into cases where it was not considered and cases where it was included. The analysis of multi-degree-of-freedom behavior confirmed that the action of multiple WECs had a substantial impact on the platform's response.

• Journal of Photoscience
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• v.9 no.2
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• pp.451-453
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• 2002

A new concept of "photo" -antisense method has been evaluated, where the inhibition of gene expression by the conventional antisense method is enhanced by photochemical binding between antisense oligonucleotides conjugated with photo-reactive compound and target mRNA or DNA. Fluorescein labeled oligodeoxyribonucleotides (F-DNA) was delivered to cell nuclei in the encapsulated form in multilamellar lecithin liposomes with neutral charge. F-DNA was previously shown to photo-bind to the complementary stranded DNA, and the delivery system using neutral liposome to be effective in normal human keratinocytes. In the present study, we used human kidney cancer G401.2/6TG.1 cell line to be advantageous in reproducible experiments. p53 was adopted as a target gene since antisense sequence information has been accumulated. The nuclear localization ofF-DNA was identified by comparing the fluorescence ofF-DNA with that of Hoechst 33258 under fluorescence microscope. After 7hr incubation to accumulate p53 protein induced by UV -B, p53 protein was quantified by Western blot. After 2hrs from F-DNA application, about 30% of cell population incorporated F-DNA in their nuclei with some morphological change possibly due to liposomal toxicity. Irradiation of visible light longer than 400nm from solar simulator at this time enhanced the inhibitory action of antisense F-DNA. The present results suggest that photo-antisense method is promising to control gene expression in time and space dependent manner. Further improvement of F-DNA delivery to cancer cells in the stability and toxicity is in progress. progress.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.39 no.3
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• pp.10-17
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• 2016

Control Chart is a graph which dots the characteristic values of a process. It is the tool of statistical technique to keep a process in controlled condition. It is also used for investigating the state of a process. Therefore many companies have used Control Chart as the tool of statistical process control (SPC). Products from a production process represent accidental dispersion values around a certain reference value. Fluctuations cause of quality dispersion is classified as a chance cause and a assignable cause. Chance cause refers unmanageable practical cause such as operator proficiency differences, differences in work environment, etc. Assignable cause refers manageable cause which is possible to take actions to remove such as operator inattention, error of production equipment, etc. Traditionally ${\bar{x}}-R$ control chart or ${\bar{x}}-s$ control chart is used to find and remove the error cause. Traditional control chart is to determine whether the measured data are in control or not, and lets us to take action. On the other hand, RNELCC (Reflected Normal Expected Loss Control Chart) is a control chart which, even in controlled state, indicates the information of economic loss if a product is in inconsistent state with process target value. However, contaminated process can cause control line sensitive and cause problems with the detection capabilities of chart. Many studies on robust estimation using trimmed parameters have been conducted. We suggest robust RNELCC which used the idea of trimmed parameters with RNEL control chart. And we demonstrate effectiveness of new control chart by comparing with ARL value among traditional control chart, RNELCC and robust RNELCC.

• Journal of Ginseng Research
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• v.41 no.3
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• pp.277-283
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• 2017

Background: The ginseng berry has various bioactivities, including antidiabetic, anticancer, antiinflammatory, and antioxidative properties. Moreover, we have revealed that the active antiaging component of the ginseng berry, syringaresinol, has the ability to stimulate longevity via gene activation. Despite the many known beneficial effects of ginseng, its effects on skin aging are poorly understood. In this study, we investigated the effects of ginseng and the ginseng berry on one of the skin aging processes, melanogenesis, and age-related pigment lipofuscin accumulation, to elucidate the mechanism of action with respect to antiaging. Methods: The human melanoma MNT1 cell line was treated with ginseng root extract, ginseng berry extract, or syringaresinol. Then, the cells were analyzed using a melanin assay, and the tyrosinase activity was estimated. The Caenorhabditis elegans wild type N2 strain was used for the life span assay to analyze the antiaging effects of the samples. A lipofuscin fluorescence assay was performed during 10 passages with the syringaresinol treatment. Results: A 7-d treatment with ginseng berry extract reduced melanin accumulation and tyrosinase activity more than ginseng root extract. These results may be due to the active compound of the ginseng berry, syringaresinol. The antimelanogenic activity was strongly coordinated with the activation of the longevity gene foxo3a. Moreover, the ginseng berry extract had more potent antiaging effects, caused a life span extension, and reduced lipofuscin accumulation. Conclusion: Taken together, our results suggest that these antimelanogenic effects and antiaging effects of ginseng berry mediate the activation of antioxidation-FoxO3a signaling.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1991.10a
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• pp.133-138
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• 1991

The aim of the present paper is to develop a computer program predicting ultimate fracture strength of initially cracked structure under monotonically increasing external loads. For this purpose, two kinds of 3-D isoparametric solid elements, one 6-node wedge element and another 8-node brick element are formulated along the small deformation theory. Plasticity in the element is checked using von Mises' yield criterion. Elasto-plastic stiffness matrix of the element is calculated taking account of strain hardening effect. If the principal strain at crack tip which is one nodal point exceeds the critical strain dependin on the material property, crack tip is supposed to be opened and the crack tip node which was previously constrained in the direction perpendicular to the crack line is released. After that, the crack lay be propagated to the adjacent node. Once a crack tip node is fractured, the energy of the newly fractured node should be released which is to be absorbed by the remaining part. The accumulated reaction force which was carried by the newly fractured node so far is then applied in the opposite direction. During the action of crack tip relief force, since unloading may be occured in the plastic element, unloading check should be made. If a plastic element unloads, elastic stress-strain equation is used in the calculation of the stiffness matrix of the element, while for a loading element, elasto-plastic stress-strain equation is continuously used. Verification of the computer program is made comparing with the experimental results for center cracked panel subjected to uniform tensile load. Also some factors affecting ultimate fracture strength of initially cracked plate are investigated. It is concluded that the computer program developed here gives an accurate solution and becomes useful tool for predicting ultimate fracture load of initially cracked structural system under monotonically increasing external loads.