• Korean Journal of Plant Resources
• /
• v.27 no.2
• /
• pp.125-132
• /
• 2014

In this study, the chemical constituents of bulb from Lilium lancifolium and Lilium tsingtauense were investigated. The content of moisture, crude protein and fiber of bulb from L. tsingtauense was higher than that of L. lancifolium, whereas the content of crude ash and nitrogen free extract of bulb from L. lancifolium was higher than that of L. tsingtauense. HPLC analysis revealed that total free sugar and amino acid content of bulb from L. tsingtauense was higher than that of L. lancifolium. There was no remarkable difference in proximate composition between the two species. The content of p-coumaric acid and ferulic acid was 1.93 mg% and 0.25 mg% in ethyl acetate fraction of L. lancifolium, on the other hand, 2.94 mg% and 0.46 mg% in ether fraction of L. tsingtauense. These results suggest that the bulb of L. tsingtauense can be considered as promising oriental medicine resource likewise L. lancifolium.

• Korean Journal of Plant Taxonomy
• /
• v.47 no.1
• /
• pp.46-50
• /
• 2017

The genus Lilium comprises about 100 species and is a major element of the family Liliaceae in temperate region of North Hemisphere. In the light of recent studies on the genus, we tried to examine the taxonomy of three Korean species (L. hansonii, L. tsingtauense, and L. distichum) belonging to section Martagon. The four available chloroplast regions (rbcL, matK, ndhF, and atpB) in Lilium species were selected and analyzed for a maximum likelihood phylogeny. As a results, three Korean species of section Martagon formed a monophyletic group. However, they nested within paraphyletic section Sinomartagon. Further, the discussions on taxonomic affinities of Korean species have made based on comparison between topology of phylogenetic tree and morphology.

• Korean Journal of Plant Resources
• /
• v.32 no.6
• /
• pp.730-734
• /
• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

• The Plant Pathology Journal
• /
• v.18 no.2
• /
• pp.85-92
• /
• 2002

A new isolate of Cucumber mosaic virus (CMV), identified as Li-CMV was isolated from a diseased Korean native lily (Lithium tsingtauense Gilg). Biological and serological properties of Li-CMV were characterized, and reverse transcription-polymerase chain reaction (RT-PCR) analysis, restriction enzyme profiling of RT-PCR products, and nucleotide sequence analysis of RNA3 of the virus were performed in this study. Remarkable differences in symptoms between Li-CMV and ordinary CMV strains were found in tobacco plants and Datura stramonium. Li-CMV-infected tobacco plants (cv. Xanthi-nc and cv. Samsun) induced chlorotic ringspots on uninoculated upper leaves, and the symptom expression was delayed or faint whereas, ordinary CMV strains induced green mosaic symptoms on the plant. Systemic infections were observed on Nicotiana benthamiana with severe mosaic symptom. Restriction mapping analysis of RT-PCR products using MspI showed that Li-CMV belonged to CMV subgroup I. A full-length CDNA copy of RNA3 for the virus was amplified by RT-PCR, cloned, and its complete nucleotide sequence was determined. The RNA3 of Li-CMV was 2, 232 nucleotides long, and consisted of two open reading frames of 843 and 657 bases encoding 3a protein (movement protein) and coat protein, respectively. Results of this study indicate that Li-CMV is a novel strain and belongs to subgroup I of CMV in the genus Cucumovirus.

• Journal of Life Science
• /
• v.15 no.4 s.71
• /
• pp.548-552
• /
• 2005

Volatile fragrance components in 5 kinds of Korean native Lilium were investigated and compared. The volatile components were extracted by SDE (simultaneous steam distillation and extraction) and identified by CC and GC-MS. As a result of the analysis of volatile aromatic ingredient of L. leichtlinii var. tigrinum Nickels., L. concolor var. parthneion Bak., L. tsingtauense Gilg., L. hansonii Leichtl., and L. amabile Palibin., using frozen materials, 60 kinds of volatile compound were identified, which were 28 aldehydes, 9 ketones, 8 alcohols, 5 esters, 5 acids, 3 furans and 2 others. The GC patterns of the aroma components of all samples resembled but the peak areas were different according to species, though all of them are Korean native Liliums.

• FLOWER RESEARCH JOURNAL
• /
• v.18 no.1
• /
• pp.62-65
• /
• 2010

This study was carried out to estimate the size and exine pattern of pollen grains in seven species of the genus Lilium by using scanning electron microscope. Exine of pollen grains, which were all monad, took the prolate forms with a pollen aperture. However, there were various L/W ratio between length (L) and width (W) of pollen grains among selected Lilium species. The pollen of L. distichum was oblong shape which had the highest L/W ratio of 2.59. On the other hand, that of L. concolor var. partheneion was broadly elliptical shape close to circle which had the lowest L/W ratio of 1.82. The surface patterns of exine were reticulate in all Lilium species that have showed diverse forms in their lumina. Pollen length and width in L. lancifolium had the greatest value, 115.90 and $46.54{\mu}m$, respectively, in comparison with those of L. tsingtauense, 70.33 and $38.72{\mu}m$, respectively. Except L. distichum and L. leichtlinii var. maximowiczii which had a similar pollen length within standard errors, the others in Lilium species showed different sizes, respectively. Therefore, these results indicated that pollen length could be a better standard for the classification of Lilium species than exine surface patterns or pollen dissection area.

• Journal of Environmental Science International
• /
• v.15 no.12
• /
• pp.1103-1107
• /
• 2006

Iris odesanensis Y.N. Lee is one kind of species among 54 endangered plant species designated by the Ministry of Environment in Korea. It is very rare native plant throughout the country. Therefore, this study was conducted to investigate the native Iris odesanensis in Mt. Naeyon for their distribution and ecological characteristics. They were mostly distributed mid-slope of the mountain from 443m to 498m altitude. They were located lower part of valley and provided enough humid condition. They had proper light and humidity conditions as they faced south south west, south south east, and south. They were also located at the places deciduous woody plants were distributed for proper light conditions for spring blooming. Iris flowered to use light at the period before the trees thickly covered with foliage. However this unfavorable light condition caused low percentage as 7.04% of blooming individuals in this study. In herbaceous, Vioia chaerophylloides(Regel) W. Hocker, Carex siderosticta Hance, Erythronium japonicum Decne., Oxalis obtriangulata Max., Hepatica asiatica Nakai, Lilium tsingtauense Gilg et cetera were appeared well. We propose that proper pruning of upper branches of the deciduous trees is needed for suitable light condition of management of native sites.

• Horticultural Science & Technology
• /
• v.29 no.2
• /
• pp.87-94
• /
• 2011

This study was carried out to investigate the effects of hot solution soaking and chilling treatment on sprouting and enlargement of bulblets obtained through in vitro culture in Korean native lilies with ornamental values. In vitro bulblets of Lilium cernuum, L. hansonii, L. hansonii for. mutatum, L. leichtlinii, and L. tsingtauense were soaked in distilled water or 100 $mg{\cdot}L^{-1}$ $GA_3$ and $GA_{4+7}$ solution maintained at $35^{\circ}C$ for two hours (hot water treatment) and/or exposed at $4^{\circ}C$ for 0, 4, or 8 weeks (chilling treatment) and then planted in plastic trays filled with media and grown in a greenhouse at $20^{\circ}C$ and under 16 h photoperiod. In all species, no bulblet propagated by tissue culture sprouted without chilling or hot water treatment due to dormancy. For dormancy breaking, $GA_{4+7}$ hot solution treatment increasing sprouting by 55-96%, whereas distilled water or GA was not effective in sprouting. Chilling treatment for 4 weeks induced sprouting by 50-70% in L. cernuum and L. leichtlinii, whereas 8 weeks was needed for sprouting of L. hansonii and L. hansonii for. mutatum. Combined treatment of hot water and chilling treatments synergistically promoted sprouting. Especially, in L. cernuum and L. hansonii, $GA_{4+7}$ hot solution soaking prior to chilling for 4 weeks promoted sprouting by 35-45% compared with the reverse order. Enlargement of bulblets resulted from increase in fresh weight and diameter was promoted by the treatments that increased the sprouting percentage of bulblets. Only in L. cernuum, shoots emerged from bulblets soaked in hot $GA_{4+7}$ solution or chilled at $4^{\circ}C$ and shoot emergence rate was highest in bulblets soaked in hot $GA_{4+7}$ solution and then chilled for 8 weeks. From these results, the most effective method for bulblet sprouting and enlargement was to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. hansonii, hansonii for. mutatum, and leichtlinii, and to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. cernuum and tsingtauense.

• Journal of Plant Biotechnology
• /
• v.46 no.3
• /
• pp.158-164
• /
• 2019

Molecular characterization of different genotypes reveals accurate information about the degree of genetic diversity that helps to develop a proper breeding program. In this study, a total of 30 EST-based simple sequence repeat (EST-SSR) markers derived from trumpet lily (Lilium longiflorum) were used across 11 native lily species for their genetic relationship. Among these 30 markers, 24 SSR markers that showed polymorphism were used for evaluation of diversity spectrum. The allelic number at per locus ranged from 1 at SSR2 locus to 34 alleles at SSR15 locus, with an average of 11.25 alleles across 24 loci observed. The polymorphic information content, PIC, values ranged from 0.0523 for SSR9 to 0.9919 for SSR2 in all 24 loci with an average of 0.3827. The allelic frequency at every locus ranged from 0.81% at SSR2 locus to 99.6% at SSR14 locus. The pairwise genetic dissimilarity coefficient revealed the highest genetic distance with a value of 81.7% was in between L. dauricum and L. amabile. A relatively closer genetic distance was found between L. lancifolium and L. dauricum, L. maximowiczii and L. concolor, L. maximowiczii and L. distichum (Jeju), L. tsingtauense and L. callosum, L. cernuum and L. distichum (Jeju ecotype), of which dissimilarity coefficient was 50.0%. The molecular fingerprinting based on microsatellite marker could serve boldly to recognize genetically distant accessions and to sort morphologically close as well as duplicate accessions.

• Journal of Plant Biotechnology
• /
• v.31 no.2
• /
• pp.95-101
• /
• 2004

This study was undertaken to study the effect of interspecific pollination of L. longiflorum and L. ${\times}$formolongi as the female parent with Oriental, Martagon and Trumpet group as the male parent by cut-style pollination. In the interspecific pollination of L. longiflorum cv. Gelria and Lorina with Oriental group as the male parent, the corresponding fruits obtained immature embryo were 1, 8, and the mean number of embryo per fruit were 11.0, 3.0, respectively. In the interspecific pollination of L. ${\times}$formolongi cv. Raizan, the corresponding fruits obtained immature embryo were 17, and the mean number of embryo per fruit were 4.0. On the other hand, in the interspecific pollination between L. longiflorum and L. ${\times}$formolongi as the female parent and Martagon and Trumpet group as the male parent, the pollination of L. ${\times}$formolongi as the female parent and L. henryi of Trumpet group as the male parent were obtained only 2 fruits, however no embryo.