Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

An Efficient in vitro Micropropagation for Production of Disease-free Bulbs in Korean Native Lilium

  • Received : 2019.11.05
  • Accepted : 2019.12.10
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

Keywords

References

  1. Askari, N., Y.G. Wang and G.J. De Klerk. 2014. In tissue culture of Lilium explants may become heavily contaminated by the standard initiation procedure. Propa. Ornam. Plants 14:49-56.
  2. Bouman, H., A. Tiekstra, E. Petutschnig, M. Homan and R. Schreurs. 2003. Cryopreservation of Lilium species and cultivars. Acta Horti. 612:147-154. https://doi.org/10.17660/actahortic.2003.612.18
  3. Chen, H., X.L. Chen, L.Q. Chen and X.X. Lu. 2007. Cryopreservation of shoot tips from in vitro plants of our flower of lily (Lilium L.) by vitrification method. Journal of Plant Genetic Resources 2:170-173.
  4. Chen, X.L., J.H. Li, X. Xin, Z.E. Zhang, P.P. Xin and X.X. Lu. 2011. Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification. S. Afr. J. Bot. 77(2):397-403. https://doi.org/10.1016/j.sajb.2010.10.005
  5. Goo, D.H., B.W. Yae and B.H. Han. 2004. In vitro propagation and enlargement of Lilium hansonii. Kor. J. Hort. Sci. Technol. 22:473-476.
  6. Guri, A.Z. and K.N. Patel. 1998. Compositions and methods to prevent microbial contamination of plant tissue culture media. U.S. Patent 5(750):402.
  7. Hirai, D. and A. Sakai. 1999. Cryopreservation of in vitrogrown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification. Plant Cell Rep. 19:150-155. https://doi.org/10.1007/s002990050725
  8. Hwang, Y.J., C.M. Song, A. Younis, C.K. Kim, Y.I. Kang and K.B. Lim. 2015. Morphological characterization under different ecological habitats and physical mapping of 5S and 45S rDNA in Lilium distichum with fluorescence in situ hybridization. Rev. Chil. Hist. Nat. 88(1):8. https://doi.org/10.1186/s40693-015-0037-3
  9. Jin, Y.H., S.H. Park, H.H. Kim and C.H. Lee. 2001. Several factors on in vitro bulblet production from native Lilium cernum and L. callosum leaf explants. Korean J. Plant Res. 14:104-105.
  10. Kaviani, B. 2011. Conservation of plant genetic resources by cryopreservation. Australian J. Crop Sci. 5: 778-800.
  11. Kim, H.H., J.Y. Lee, N.Y. Noh, G.T. Cho, M.S. Yoon, H.J. Baek and J.K. Kim. 2010. Development of cryopreservation protocols through droplet-vitrification and its application to vegetatively propagated crop germplasm. 2010 Annual Conference of The Plant Resources Society of Korea. Hwaseong, Korea. p.12.
  12. Kim, K.W., J.D. Choi, K.I. Park and B.Y. Choi. 1995. Multiplication and growth of bulblets regenerated from scale of Lilium lancifolium Thunb. in vitro. J. Kor. Flower Res. 4:19-26.
  13. Kim, T.J. 2008. Wild flowers and resources plants in Korea. SNU Press, Seoul, Korea. p. 1-5.
  14. Leunufna, S. and E.R.J. Keller. 2003. Investigating a new cryopreservation protocol for yams (Dioscorea spp.). Plant Cell Rep. 21:1159-1166. https://doi.org/10.1007/s00299-003-0652-3
  15. Matsumoto, T., A. Sakai and K. Yamada. 1995. Cryopreservation of in vitro-grown apical meristems of lily by vitrification. Plant Cell Tiss. Org. 41:237-241. https://doi.org/10.1007/BF00045087
  16. Matsumoto, T. 2017. Cryopreservation of plant genetic resources: conventional and new methods. Rev. Agric. Sci. 5:13-20. https://doi.org/10.7831/ras.5.13
  17. Niedz, R.P. 1998. Using isothiazolone biocides to control microbial and fungal contaminants in plant tissue cultures. Hort. Technology 8:598-601. https://doi.org/10.21273/HORTTECH.8.4.598
  18. Paek, K.Y. and S.H. Shin. 1983. Factors affecting regeneration ability and physiology of dormancy in the mature bulbil segments of Lilium lancifolium in vitro. J. Kor. Soc. Hort. Sci. 24:149-157.
  19. Park, J.Y., Y.K. Yoo, J.H. Jeong and K.S. Kim. 1998. Effect of light and scale explant conditions on propagation efficiency in Lilium callosum scale culture. Kor. J. Hort. Sci. Technol. 10:358-360.
  20. Thakur, R. and A. Sood. 2006. An efficient method for explant sterilization for reduced contamination. Plant Cell Tiss. Org. 84:369-371. https://doi.org/10.1007/s11240-005-9034-6
  21. Wang, Q.C. and A. Perl. 2006. Cryopreservation in floricultural plants: In Teixeira de Silva, J.A. (ed.), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Global Science Book, London, UK. pp. 524-539.
  22. Wang, Q.C. and J.P.T. Valkonen. 2009. Cryotherapy of shoot tips: novel pathogen eradication method. Trends Plant Sci. 14:119-122. https://doi.org/10.1016/j.tplants.2008.11.010
  23. Xia, Y., X. Deng, P. Zhou, K. Shima and J. Teixeira da Silva. 2006. The world floriculture industry: dynamics of production and markets: In Teixeira da Silva, J.A. (ed.), Floriculture, Ornamental and Plant Biotechnology, Advances and Topical Issues, Volume IV, Global Science Books London, UK. pp. 336-347.
  24. Yi, J.Y., K. Balaraju, H.J. Baek and M.S. Yoon. 2018. Cryopreservation of Citrus limon (L.) Burm. F shoot tips using a droplet-vitrification method. Korean J. Plant Res. 31(6): 684-694. https://doi.org/10.7732/kjpr.2018.31.6.684
  25. Zhang, Y.Q., X.X. Li, Q. Ma, H.P. Wang and E. Shen. 2004. Preliminary study on vitrification cryopreservation technology of edible lily germplasm. China Vegetables 4:11-13.

Cited by

  1. Enhancing in vitro Growth of Bulbs for Mass Propagation of Lily Germplasm vol.34, pp.1, 2021, https://doi.org/10.7732/kjpr.2020.34.1.017