Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Gametophyte Propagation and Sporophyte Formation of Asian Chain Fern [Woodwardia japonica (L. f.) Sm.] Under Various Medium Conditions In Vitro and Ex Vitro

  • Cho, Ju Sung (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Jang, Bo Kook (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Park, Kyungtae (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Lee, Ha Min (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University)
  • Received : 2019.10.16
  • Accepted : 2019.12.03
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated a suitable method that could be applied for Asian chain fern [Woodwardia japonica (L. f.) Sm.] to propagate gametophytes and promote sporophyte formation. The gametophytes used in all experiments were obtained from germinated spores in vitro and were subcultured at 8-week intervals. The most appropriate media for gametophyte propagation was identified by culturing 300 mg of gametophyte in Murashige and Skoog (MS) basal medium (1/8, 1/4, 1/2, 1, 2), and Knop medium for 8 weeks. As a result, fresh weight of the gametophyte was increased by 56.7-fold on MS medium. Moreover, antheridium formation as well as gametophyte growth was improved on MS medium, especially. To improve the sporophyte formation ex vitro, 1.0 g of gametophyte was ground with distilled water and spread on eight combinations onto four different culture mediums, such as bed soil, peat moss, perlite and decomposed granite. Then generation and growth of sporophytes were investigated after cultivation for 10 weeks. As a result of this experiment, peat moss had a promotive effect of sporophyte formation at single-use and mixed culture soils. In particular, a mixture of bed soil, peat moss and perlite in a 1:1:1 ratio (v/v/v) led to the accelerated formation (782.5 ea/pot) and the frond growth of sporophytes. This included increases in length and width of fronds. However, promotive effect of gametophyte growth and sporophyte formation was not found at single-use and treatment with high ratio of bed soil.

Keywords

References

  1. Biodiversity of Korea (BK) 2019. National Institute of Biological Resources, Incheon, Korea. Available via https://species.nibr.go.kr/index.do (accessed data: 8 Oct. 2019).
  2. Carafa, A.M. 1990. Gametophyte development of Woodwardia radicans (L.) SM.: effect of population density and antheridiogen on sex expression. Giorn. Bot. Ital. 124:571-580. https://doi.org/10.1080/11263509009430257
  3. Cho, J.S. and C.H. Lee. 2017. Several factors affecting mass production of Microlepia strigosa (Thunb.) C. Presl sporophytes. Korean J. Hortic. Sci. 35:46-58 (in Korean).
  4. Cho, J.S., J.H. Han and C.H. Lee. 2017. Effects of medium components and composition on mass propagation of Arachniodes aristata (G. Forst.) Tindale. Korean J. Hortic. Sci. 35:131-141 (in Korean).
  5. Choi, J.M., J.W. Ahn, J.H. Ku and Y.B. Lee. 1997. Effect of medium composition on physical properties of soil and seedling growth of red-pepper in plug system. J. Korean Soc. Hort. Sci. 38:618-624 (in Korean).
  6. Fernandez, H., A.M. Bertrand and R. Sanchez-Tames. 1996. Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis. Plant Cell Tiss. Org. Cult. 45:93-97. https://doi.org/10.1007/BF00043434
  7. Fernandez, H. and M.A. Revilla. 2003. In vitro culture of ornamental ferns. Plant Cell Tiss. Org. Cult. 73:1-13. https://doi.org/10.1023/A:1022650701341
  8. Jang, B.K., J.S. Cho and C.H. Lee. 2019a. Propagation methods for gametophyte proliferation and sporophyte formation in silver cloak fern (Cheilanthes argentea). Hortic. Environ. Biotechnol. 60:435-442. https://doi.org/10.1007/s13580-019-00128-6
  9. Jang, B.K., J.S. Cho, K.T. Park and C.H. Lee. 2019b. A methodology for large-scale Athyrium sheareri gametophyte proliferation and sporophyte production using tissue culture. In Vitro Cell. Dev. Biol. Plant. 55:519-526. https://doi.org/10.1007/s11627-019-09991-5
  10. Korea Biodiversity Information System (KBIS) 2019. Korea National Arboretum. Pocheon, Korea. Available via http://nature.go.kr/kbi/plant/pilbk/selectPlantPilbkDtl.do (accessed data: 8 Oct. 2019).
  11. Kwon, H.J., J.H. Han, C.H. Lee and S.Y. Kim. 2017. Conditions of in vitro spore germination and prothallium culture for sporophyte propagation of Polystichum braunii (Spenn.) Fee. J. Plant Biol. 44:454-461 (in Korean).
  12. Lee, J.S. 2001. Several factors affecting sporophyte formation of three species in Pteridophyta. MS Thesis, Chungbuk National University, Cheongju, Korea.
  13. Lee, S.I., B.K. Jang and C.H. Lee. 2019. Effect of medium and soil conditions on propagation of gametophyte and sporophyte in Leptogramma pozoi (Lag.) Ching subsp. mollissima (Fisch. ex Kunze) Nakaike. Korean J. Plant Res. 32:290-295 (in Korean).
  14. Menendez, V., M.A. Revilla, P. Bernard, V. Gotor and H, Fernandez. 2006. Gibberellins and antheridiogen on sex in Blechnum spicant L. Plant Cell Rep. 25:1104-1110. https://doi.org/10.1007/s00299-006-0149-y
  15. Miller, J.H. 1968. Fern gametophytes as experimental material. Bot. Rev. 34:361-440. https://doi.org/10.1007/BF02859133
  16. Shin, S.L. and C.H. Lee. 2009. In vitro medium composition and culture method affecting mass propagation of Osmunda japonica Thunb. gametophytes. Korean J. Hortic. Sci. 27: 299-304.
  17. Shin, S.L., M.Y. Lee, J.S. Choi and C.H. Lee. 2009. Effect of medium components and culture methods on gametophyte propagation of Pteridium aquilinum var. latiusculum (Desv.) Underw. ex Hell. Korean J. Plant Res. 22:337-342.
  18. Shorina, N.I. 2001. Population biology of gametophytes in homosporous Polypodiophyta. Russ. J. Ecol. 32:164-169. https://doi.org/10.1023/A:1011306010154
  19. Yamane, H. 1998. Fern antheridiogens. Int. Rev. Cytol. 184:1-32. https://doi.org/10.1016/S0074-7696(08)62177-4