The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization and Sequence Analysis of a Lily Isolate of Cucumber mosaic virus from Lithium tsingtauense

  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University) ;
  • Park, Hye-Won (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University) ;
  • Park, Jang-Kyung (Division of Biological Environment, Kangwon National University)
  • Published : 2002.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

A new isolate of Cucumber mosaic virus (CMV), identified as Li-CMV was isolated from a diseased Korean native lily (Lithium tsingtauense Gilg). Biological and serological properties of Li-CMV were characterized, and reverse transcription-polymerase chain reaction (RT-PCR) analysis, restriction enzyme profiling of RT-PCR products, and nucleotide sequence analysis of RNA3 of the virus were performed in this study. Remarkable differences in symptoms between Li-CMV and ordinary CMV strains were found in tobacco plants and Datura stramonium. Li-CMV-infected tobacco plants (cv. Xanthi-nc and cv. Samsun) induced chlorotic ringspots on uninoculated upper leaves, and the symptom expression was delayed or faint whereas, ordinary CMV strains induced green mosaic symptoms on the plant. Systemic infections were observed on Nicotiana benthamiana with severe mosaic symptom. Restriction mapping analysis of RT-PCR products using MspI showed that Li-CMV belonged to CMV subgroup I. A full-length CDNA copy of RNA3 for the virus was amplified by RT-PCR, cloned, and its complete nucleotide sequence was determined. The RNA3 of Li-CMV was 2, 232 nucleotides long, and consisted of two open reading frames of 843 and 657 bases encoding 3a protein (movement protein) and coat protein, respectively. Results of this study indicate that Li-CMV is a novel strain and belongs to subgroup I of CMV in the genus Cucumovirus.

Keywords

References

  1. Allen, T. C. 1972. Lily symptomless virus. CMI/AAB Descriptions of Plant Viruses No. 96, Kew, United Kingdom
  2. Anonymous, 1998. Detection and biodiversity of cucumber mosaic cucumovirus. J. Plant Pathol. 80:133-149
  3. Beattie, R. B. and Whittle, M. J. 1993. Doppler and fetal growth retardation. Arch. Dis. Child 69:271-273 https://doi.org/10.1136/adc.69.3_Spec_No.271
  4. Chen, Y. K., Derks, A. F. L. M., Langeveld, S., Goldbach, R. and Prins, M. 2001. High sequence conservation among cucumber mosaic virus isolates from lily. Arch. Virol. 146:1631-1636 https://doi.org/10.1007/s007050170085
  5. Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. J. Virol. Methods 83:67-73 https://doi.org/10.1016/S0166-0934(99)00106-8
  6. Ding, S. W., Li, W. X. and Symons, R. H. 1995. A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J. 14:5762-5772
  7. Gonda, T. J. and Symons, R. H. 1978. The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88:361-370 https://doi.org/10.1016/0042-6822(78)90292-1
  8. Jung, H. J., Ueda, S., Ryu, K. H., Lee, S. Y. and Choi, J. K. 2000. A novel strain of cucumber mosaic virus isolated from Lilium longiflorum. Plant Pathol. J. 16:306-311
  9. Kaper, J. M. and Waterworth, H. E. 1981. Cucumoviruses, In: Plant Virus Infections and Comparative Diagnosis, pp. 257-332. Ed. E. Karstak, New York and Amsterdam
  10. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680-685 https://doi.org/10.1038/227680a0
  11. Li, Q., Ryu, K. H. and Palukaitis, P. 2000. Cucumber mosaic virus-plant interactions: Identification of 3a protein sequences affecting infectivity, cell-to-cell movement, and long-distance movement. Mol. Plant-Microbe Interact. 14:378-385 https://doi.org/10.1094/MPMI.2001.14.3.378
  12. Mayers, C. N., Palukaitis, P. and Carr, J. P. 2000. Subcellular distribution analysis of the cucumber mosaic virus 2b protein. J. Gen. Virol. 81:219-226 https://doi.org/10.1099/0022-1317-81-1-219
  13. Owen, J. and Palukaitis, P. 1988. Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 166:495-502 https://doi.org/10.1016/0042-6822(88)90520-X
  14. Owen, J., Shintaku. M., Aeschleman, P.. Ben Tahar, S. and Palukaitis, P. 1990. Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA3. J. Gen. Virol. 71:2243-2249 https://doi.org/10.1099/0022-1317-71-10-2243
  15. Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41:281-348 https://doi.org/10.1016/S0065-3527(08)60039-1
  16. Rizos, H., Gunn, L. V., Pares, R. D. and Gillings, M. R. 1992. Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. J. Gen. Virol. 73:2099-2113 https://doi.org/10.1099/0022-1317-73-8-2099
  17. Roossinck, M. J. 1998. Plant virology protocols, pp. 189-196, Humana Press, New Jersey
  18. Roossinck, M. J., Zhang, L. and Hellwald, K. H. 1999. Rearrangements in the 5' nontranslated region and phylogenetic analysis of cucumber mosaic vims RNA3 indicate radial evolution of three subgroups. J. Virol. 73:6752-6758
  19. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory Press, New York
  20. Sanger, F., Niklen, S. and Colson, A. R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Nat. Acad. Sci. USA 74:5463-5467 https://doi.org/10.1073/pnas.74.12.5463

Cited by

  1. Cloning of the 5'-end and Amplification of Full-Length cDNA of Genomic RNA of Lily symptomless virus vol.18, pp.4, 2002, https://doi.org/10.5423/PPJ.2002.18.4.187
  2. Cucumber mosaic virus groups IA and II are represented among isolates from naturally infected lilies vol.127, pp.3, 2010, https://doi.org/10.1007/s10658-010-9600-6
  3. Mapping the sequence domain for systemic infection in edible lily on the viral genome of Cucumber mosaic virus vol.71, pp.5, 2005, https://doi.org/10.1007/s10327-005-0213-4
  4. Influence of virus infection in quality of production of Lilium x fomolongi as cut-flowers vol.9, pp.4, 2003, https://doi.org/10.5423/RPD.2003.9.4.237
  5. A genetically novel, narrow-host-range isolate of cucumber mosaic virus (CMV) from rosemary vol.161, pp.7, 2016, https://doi.org/10.1007/s00705-016-2874-z
  6. A rapid immunochromatographic test to detect the lily mottle virus vol.220, 2015, https://doi.org/10.1016/j.jviromet.2015.04.010
  7. Simultaneous detection of three lily viruses using Triplex IC-RT-PCR vol.249, 2017, https://doi.org/10.1016/j.jviromet.2017.08.018
  8. Plant virus cDNA chip hybridization for detection and differentiation of four cucurbit-infecting Tobamoviruses vol.110, pp.1, 2003, https://doi.org/10.1016/S0166-0934(03)00082-X
  9. Involvement of RNA2 for systemic infection of Cucumber mosaic virus isolated from lily on zucchini squash vol.97, pp.1, 2003, https://doi.org/10.1016/S0168-1702(03)00215-6
  10. Occurrence of Mosaic Disease of Hosta Plane Caused by Hosta virus X vol.18, pp.6, 2002, https://doi.org/10.5423/PPJ.2002.18.6.313
  11. Characterization of Cucumber mosaic virus Isolated from Water Chickweed(Stellaria aquatica) vol.20, pp.2, 2004, https://doi.org/10.5423/PPJ.2004.20.2.131
  12. Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method vol.163, pp.2, 2018, https://doi.org/10.1007/s00705-017-3618-4
  13. pp.00320862, 2018, https://doi.org/10.1111/ppa.12940