• Title/Summary/Keyword: LU5

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Determination of L-Dopa by Spectrofluorimetry Using Co-fluorescence Enhancer (보조 형광증가제를 이용한 L-Dopa의 형광분광법적 정량)

  • Lee, Sang Hak;Ahn, Jung Mi
    • Journal of the Korean Chemical Society
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    • v.44 no.6
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    • pp.541-546
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    • 2000
  • Methods to determine L-dopa(L-3,4-dihydroxyphenylalanine) in aqueous solution by spectrofluorimetry based upon the ligand sensitized luminescence of Tb(III) ion L-dopa complex have been studied. Tb(III) ion and Lu(III) ion were used as ligand sensitized fluorescencer and co-fluorescence enhancer, respectively. The effects of excitation wavelength, pH, concentration of Tb(III) ion, concentration of Lu(III) ion and emission wavelength on the fluorescence intensity were investigated. The fluorescence intensity of the Tb(III) ion L-dopa complex was further increased with addition of Lu(III) ion. The calibration curve for L-dopa was linear over the range from 5.0 ${\times}$ $10^{-7}$ M to 1.0${\times}$ $10^{-4}$ M and the detection limit was 4.0 ${\times}$ $10^{-8}$ M under the optimal experimental conditions of 300 nm, 8.0, 1.0 ${\times}$ $10^{-4}$ M and 545 nm for excitation wavelength, pH, concentration of Tb(III) ion and emission wavelength, respectively. When Lu(III) ion was added to Tb(Ⅲ) ion L-dopa complex, the concentration range of linear response and detection limit were 1.0${\times}$$10^{-8}$ M to 2.0 ${\times}$ $10^{-4}$ M and 1.0 ${\times}$ $10^{-9}$ M, respectively under the optimal experimental conditions of 300 nm, 8.5, 1.0 ${\times}$ $10^{-5}$ M, 1.0 ${\times}$ $10^{-5}$ M, 545 nm for excitation wavelength, pH, concentration of Tb(III) ion, concentration of Lu(III) ion and emission wavelength, respectively.

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Effects of Acupuncture at the River Points(LU8, HT4, PC5, SP5, KI7, LR4) on the Changes of Nitric Oxide Synthase and Norepinephrine in Rats (백서(白鼠)의 경혈(經穴)(LU8, SP5, HT4, KI7, PC5, LR4) 침자(針刺)가 Nitric Oxide Synthase 및 Norepinephrine의 변화에 미치는 영향(影響))

  • Kim, Youngsun;Choi, Donghee;Jang, Hosun;Na, Changsu;Shin, Heontae;Cho, Joohyun;Lee, Kyoungin;Kim, Sunmin;Pyo, Byoungsik;Youn, Daehwan
    • Korean Journal of Acupuncture
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    • v.30 no.1
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    • pp.37-46
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    • 2013
  • Objectives : The Meridians and acupuncture points are the fundamental theories for acupuncture therapy. They have been associated with nervous system, but It is not well defined. We investigated that the effects of acupuncture at the river points(LU8, HT4, PC5, SP5, KI7, LR4) on the changes in the expression of nNOS, iNOS, eNOS, and NE in rats. Methods : The Male Sprague-Dawley rats were divided into 6 groups each non-acupuncture and acupuncture group. We inserted needle and retained for 5 minutes on both left and right sides of LU8, HT4, PC5, SP5, KI7, LR4 which were the river points of five transport points for 6 yin meridian vessels. After that, blood was drawn via cardiac puncture, and tissues for each point near meridian vessels were extracted to examine the changes in the changes of nNOS, eNOS, iNOS and NE. Results : The LU8 and HT4 group showed a significant decrease on nNOS. In terms of eNOS and iNOS, the LU8 group decreased significantly while the KI7 group increased significantly. However, the experimental groups didn't show any significant changes on the plasma and tissue norepinephrine without plama NE in SP5 group. Conclusions : The effect on the nNOS, iNOS, eNOS of acupuncture at LU8 and KI7 could be observed, and it is considered that the effect of acupuncture related with on nervous system could be studied by additional researches based on this one.

Effects of acupuncture at the $LU8{\cdot}K17$ on Anti-apoptotic cell death and neuroprotection in Rat hippocampus following focal brain ischemic injury induced by Intraluminal Filament insertion in Rats (직자(直刺), 영수(迎隨) 및 염전수기법(捻轉手技法)에 따라 시행한 경거(經渠)${\cdot}$복류(復溜) 침자(鍼刺)가 중대뇌동맥(中大腦動脈) 폐새(閉塞)에 의하여 유발(誘發)된 국소(局所) 뇌허혈(腦虛血) 백서(白鼠) hippocampus의 항세포자멸사(抗細胞自滅死) 및 복경보호(福經保護)에 미치는 영향(影響))

  • Youn, Dae-Hwan;Byun, Jeng-Yun;Choi, Chan-Hun;Baek, Jin-Ung;Jeong, Ji-Yeon;Jung, Yeon-Jin;Na, Chang-Su
    • Korean Journal of Acupuncture
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    • v.24 no.3
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    • pp.205-221
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    • 2007
  • Objectives : Aims of this study is to investigate the effects of $LU8{\cdot}KI7$ in rat induced by experimental focal ischemia. Materials and methods : The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The groups divided into 6groups, control; no therapy group after ischemia-induced, AT1; acupuncture therapy group at $LU8{\cdot}KI7$ after ischemia-induced, AT2; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 9times, AT3; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 9times, AT4; acupuncture therapy at$LU8{\cdot}KI7$ bilaterally and the needle was inserted to the direction following the flowing route of the meridian(digital direction), AT5; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally, the needle was inserted to the direction following the flowing route of the meridian(digital direction) and the needle was twirled and rotated forward with the thumb of the right hand 9times. Acupuncture therapy was carried out 7times during 2weeks after focal ischemia-induced. The anti-apoptotic and neuroprotective effects of acupuncture are observed by Bax, Bcl-2, mGluR5, cytochrome c, Cresyl violet and ChAT-stain. Results : The intensity of Bax was decreased in AC1, AC4, AC5 group, was increased in AC2, AC3 group. The intensity of Bcl-2 was increased in AC2, AC3, AC4, AC5 group. The intensity of mGluR5 was decreased in AC1 group, was increased in AC4, AC5 group. The intensity of Cytochrome c was increased in ACI, AC2 group, was decreased in AC4, AC5 group. The density of neurons stained by Cresyl violet was increased in all group without control group. The density of ChAT was increased in AC2, ACS group. Conclusions : Our study suggests that AC5 group show anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.

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Creatine Kinase and its Isoenzymes in Jindo Dogs (진도견의 creatine kinase 활성치와 isoenzyme)

  • 최석화
    • Journal of Veterinary Clinics
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    • v.15 no.1
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    • pp.41-45
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    • 1998
  • This study was performed to investigate the serum creative kinase(CK) activity and CK isoenzyme in Jindo dog. Serum CK activity and CK-isoenzyme were analyzed in 53 Jindo dogs of both sexes. The mean value and normal range of serum CK activity were 24.1 lu/$\ell$ and 7-91 lu/$\ell$, respectively, in 29 female dogs, 24.8 lu/$\ell$ and 8-89 lu/$\ell$ in 24 male dogs. The CK activity of the Puppy showed a tendency to be higher than that of the adult. There was no significance between Puppy and adult. Three isoenzymes (CK-MM, CK- BB, and CK-MB) were recognized in serum. The mean percentages of female and male were as follows: 48.31fp and 48.1% for CK-MM, 35.49) and 33.61fp for CK-BB, and 8.2% and 10.1% for CK-MB in the puppy and 46.21% and 46.1 % for CK-MM, 36.3% and 37.6% for CK-BB and 10.5% and 9.5% for CK-MB in the adult.

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Hypersonic Chemical Nonequilibrium Flow Analysis with HLLE+ and LU-AF (HLLE+와 LU-AF를 이용한 극초음속 화학적 비평형 유동장 해석)

  • Park Soo-Hyung;Kwon Jang Hyuk
    • Journal of computational fluids engineering
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    • v.5 no.2
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    • pp.47-54
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    • 2000
  • A robust Navier-Stokes code has been developed to efficiently predict hypersonic flows in chemical nonequilibrium. The HLLE+ flux discretization scheme is used to improve accuracy and robustness of hypersonic flow analysis. An efficient LU approximate factorization method is also used to solve the flow equations and species continuity equations in fully coupled fashion to implicitly treat stiff source terms of chemical reactions. The HLLE+ scheme shows lower grid dependency for the wall heating rates than other schemes. The developed code has been used to compute chemical nonequilibrium air flow through expanding hypersonic nozzle and past two and three dimensional blunt-nosed bodies. The results are in good agreement with existing numerical and experimental results.

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The modern Chinese literature and Freudian psychoanalysis - Focuing acceptance of the Freud's theory psychoanalysis on Lu xun and Guo Mo ruo (중국 신문학 초기의 프로이드 정신분석학 수용 - 루쉰(魯迅)과 궈모뤄(郭沫若)의 프로이드 정신분석학 이론 수용을 중심으로)

  • Ko, hae-kyung
    • Cross-Cultural Studies
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    • v.37
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    • pp.81-107
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    • 2014
  • Freud's' discovery of the unconscious' is a great revolution. Because of this, human beings were able to get the opportunity to look into their inner than honest. Lu Xun and Guo Mo Ruo a pair of realism and romanticism literature representative of Chinese modern literature writers. They then absorb the psychoanalytic theory o Freud along with other Western zeitgeist, introduce them widely inliterary theory and creative practice, which was again. Lu Xun was reflected in the human world of the unconscious Freud uncovered on the basis of strict realism literary spirit, Guo Mo Ruo is in accordance with the romantic literary time for the purpose of 'art for art' depicting a man's inner psychological well did. Although Freud spirit they claim to social and literary artistry of literature based on different yarns in the Acceptance of analytical theory, has the characteristics of a common sentiment analysis method to express and describe the unconscious human potential. 5.4 When Lu Xun and Guo Mo Ruo China Journalism writers, including Freud's psychoanalytic theory has embraced the Enlightenment and the old feudal society was a major contribution to want to read exactly what the human inner hearing. Chinese modern literature writers have to accept sometimes positive, sometimes accepting the psychoanalytic theory was intellectually sharp criticism, which could be a great instrument in time to the 5.4 Enlightenment and psychological fiction novel further development.

The luminescence properties of Eu3+ or Tb 3+ doped Lu2Gd1Ga2Al3O12 phosphors for X-ray imaging

  • M.J. Oh;Sudipta Saha;H.J. Kim
    • Nuclear Engineering and Technology
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    • v.55 no.12
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    • pp.4642-4646
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    • 2023
  • The Tb3+ or Eu3+-doped Lu2Gd1Ga2Al3O12 phosphor were fabricated by funace at 1500 ℃ for 12 h using a solid state reaction. The XRD (X-ray diffraction_Panalytical X'Pert Pro) and FE-SEM (field emission scanning electron microscope) are measured to confirm the crystalline structure and surface morphology of the phosphor. The Tb3+-doped Lu2Gd1Ga2Al3O12 phosphor emits the lights in 470~650 nm wavelength range due to transitions from 5D4 to 7Fj. Therefore, it shows the green region in the CIE chromaticity diagram under both UV and X-rays excitations. The Eu3+-doped Lu2Gd1Ga2Al3O12 phosphor emits the lights in 550~750 nm wavelength range because of 5Di to 7Fj. The emission is confirmed to be in the red region using the CIE chromaticity diagram. The Tb3+ or Eu3+-doped Lu2Gd1Ga2Al3O12 phosphor shows the characteristic f-f transition with a long decay time, which is about several milliseconds. They have the high efficiency of light emission for X-ray because of their high effective Z number (Zeff = 58.5) and density. Therefore, they are very much promising phosphors for X-ray imaging application in medical fields.

BAG5 regulates PTEN stability in MCF-7 cell line

  • Zhang, Ying;Gao, Haiyan;Gao, Haidong
    • BMB Reports
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    • v.46 no.10
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    • pp.490-494
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    • 2013
  • The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a tumor-suppressing lipid phosphatase that is frequently absent in breast tumors. Thus, the stability of PTEN is essential for tumor prevention and therapy. The ubiquitin-proteasome pathway has an important role in regulating the functions of PTEN. Specifically, carboxyl terminus Hsp70-interacting protein (CHIP), the E3 ubiquitin ligase of PTEN, can regulate PTEN levels. In this study, we report that BCL-2-associated athanogene 5 (BAG5), a known inhibitor of CHIP activity, reduces the degradation of PTEN and maintains its levels via an ubiquitylation-dependent pathway. BAG5 is identified as an antagonist of cell tumorigenicity.

Crystal Structure Refinement and Persistent Luminescence Properties of Lu3Al5-xGaxO12:Ce3+,Cr3+ Phosphors (Lu3Al5-xGaxO12:Ce3+,Cr3+ 형광체의 결정구조 분석 및 잔광성 발광 특성)

  • Kim, Ji-Won;Kim, Yeong-Jin
    • Korean Journal of Materials Research
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    • v.30 no.8
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    • pp.413-420
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    • 2020
  • Lu3Al5-xGaxO12:Ce3+,Cr3+ powders are prepared using a solid-state reaction method. To determine the crystal structure, Rietveld refinement is performed. The results indicate that Ga3+ ions preferentially occupied tetrahedral rather than octahedral sites. The lattice constant linearly increases, obeying Vegard's law, despite the strong preference of Ga3+ for the tetrahedral sites. Increasing x led to a blue-shift of the Ce3+ emission band in the green region and a change in the emission intensity. Persistent luminescence is observed from the powders prepared with x = 2-3, occurring through a trapping and detrapping process between Ce3+ and Cr3+ ions. The longest persistent luminescence is achieved for x = 2; its lifetime is at least 30 min. The findings are explained using crystal structure refinement, crystal field splitting, optical band gap, and electron trapping mechanism.

Advanced Program-to-Program Communication (APPC) 기능 분석

  • Lee, Mi-Seon;Nam, Sang-Woo;Son, Jin-Woo
    • Electronics and Telecommunications Trends
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    • v.5 no.4
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    • pp.120-133
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    • 1990
  • APPC는 프로그램과 프로그램 사이의 통신을 위해 1982년 IBM에 의해 소개되었다. APPC의 구조적인 기초는 logical unit 6.2이다. 두 사용자가 서로 통신을 하려면 먼저 LU 사이의 세션(session)이 이루어져야 한다. LU 6.2에서의 기본은 conversation 개념이다. conversation은 통신하는 두 프로그램 사이의 연결로 두개의 통신하는 프로그램을 대표하는 두 LU 사이의 세션을 이용한다. conversation은 세션의 serial time slice이다.