• Microbiology and Biotechnology Letters
• /
• v.32 no.3
• /
• pp.206-211
• /
• 2004

Levansucrase gene(lscA) from Pseudomonas aurantiaca was subcloned downstream of GAL1 promoter in pYES 2.0 and pYInu-AT [GAL10 promoter ＋ exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-lscA and p YInu-lscA, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Sacchromayces cerevisiae SEY2102, and transformants with high activity of levansucrase were selected. When each yeast transform ants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 8.62 U/ml with the strain harboring pYES-lscA and 5.43 U/ml with the strain harboring pYInu-lscA. The levansucrase activity of 80% was detected in the periplasmic space and cytoplasm. The levansucrase activity in the medium of SEY2102/pYInu-lscA was 0.87 U/ml whereas that of SEY2102/pYES-lscA was 0.47 U/ml, which implying the exoinulinase signal sequence didn't enhance the secretion efficiency of levansucrase. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.

• Journal of Life Science
• /
• v.12 no.6
• /
• pp.745-751
• /
• 2002

Several bacterial strains producing biosurfactants were isolated from polluted marine and soil by oil. One of the strains named LSC11 showed strong production activity of biosurfactants. This strain was identified as a Bacillus sp. LSC11 based on the morphological, biochemical, and physiological characteristics. The biosurfactant, produced by the strain, emulsified crude oil, vegetable oil, and hydrocarbons. The surface tension of the culture broth of Bacillus sp. LSC11 decreased to 32 mN/m. The crude biosurfactant was obtained from the culture broth by acid precipitation, freeze drying, solvent extraction, and evaporation. The emulsifying activity of the biosurfactant showed better than the chemically synthesized surfactant (SDS, Span40, Span 85). The biosurfactants had strong properties as an emulsifying agent and as an emulsion-stabilizing agent.

• Journal of Korean Society of Forest Science
• /
• v.88 no.1
• /
• pp.18-30
• /
• 1999

This study was carried out to compare the characteristics of hydraulic architecture by measurement of leaf specific conductivity(LSC) in the twenty-one deciduous broad-leaved species. The LSC's of stems were higher than in branches except Acer mono, A. triflorum, Betula schmidtii, Fraxinus rhynchophylla and Zelkova serrata, and lowest in junction parts as compared with stems and branches. In diffuse-porous species, the LSC was much higher in B. plalyphylla var. iaponica, B. schmidtii, Cornus controversa, Tilia amurensis than those of other species and especially lowest in A. triflorum. In ring-porous species, the LSC was much higher in Maackia amurensis, Paulownia tomentasa, Quercus aliens, Q. serrata than those of other species and lowest in Ulmus davidiana var. japonica. The LSC's of 1-year-old terminal shoots of stem(the leader and adjacent laterals) in A. triflorum, C. controversa. F. mandshurica, Q. mongolica and Z. serrata ranged from 22 to $139{\mu}{\ell}/g$ in the leaders, and 11 to $73{\mu}{\ell}/g$ in the adjacent laterals. The LSC's of leaders were usually greater than the adjacent laterals because of the hydraulic dominance of the leader shoot.

• Journal of Korean Society of Rural Planning
• /
• v.2 no.2
• /
• pp.45-56
• /
• 1996

Land suitability classification(LSC) is an appraisal and grouping(or the process of appraisal anti grouping) of specific tracts(of land) in terms of their relative land suitability for a definEd use, and is one of the land use planning techniques. This paper reviews the selected studies on LSC whose purposes are to especially contribute land use planning in case of Japan, So, this study examines the LSC's application to Korea based on Japanes LSC studies with a view to development of the methods on LSC for rational land use planning in our rural area. The result resolves itself into Table 2. However, it is undesirable to borrow from Japanes LSC studies like that, because of the difference of administrative,geographical,traditional,social and economical conditions. Therefore, it is necessary that the many case studies and examinations should be carried out in order to develop the methods on LSC suitable to Korean actual circumstances.

• Journal of the Korean Society for Precision Engineering
• /
• v.8 no.3
• /
• pp.18-26
• /
• 1991

A study on the measuring and evaluation of LSC(Least Square Center) roundness was carried out. The experimental set-up was made by index head and indicator, and the measuring data were compensated by a developed computer program. The results obtained are as follows : 1) An index head can conveniently be used to measure LSC roundness. 2) A program for calculating LSC roundness is developed. 3) Without a high quality roundnes measuring apparatus, LSC roundness can be measured and calculated by using index head and the developed program in machine shop as well as in a measuring room

• The Korean Journal of Nuclear Medicine Technology
• /
• v.27 no.2
• /
• pp.95-98
• /
• 2023

Purpose: The precision error of a bone density meter reflects the equipment and reproducibility of results by an examiner. Precision error values can be expressed as coefficient of variation (CV), CV%, and root mean square-SD (RMS-SD). The International Society for Clinical Densitometry (ISCD) currently recommends using RMS-SD as the precision error value. When a 95% confidence interval is applied, the least significant change (LSC) value is calculated by multiplying the precision error value by 2.77. Exceeding the LSC value reflects a significant difference in measured bone density. Therefore, the LSC value of a bone density equipment is an essential factor for accurately determining a patient's bone density. Accordingly, we aimed to calculate the LSC value of a bone density meter (Lunar iDXA, GE) and compare it with the value recommended by the ISCD. We also assessed whether the value measured by the iDXA equipment was below the LSC value recommended by ISCD. Material and Methods: The bone densities of the lumbar spine and thighs of 30 participants were measured twice, and the LSC values were calculated using the precision calculation tool provided by the ISCD (http://www.iscd.org). To check the reproducibility of the measurement, patients were asked to completely dismount from the equipment after the first measurement; the patient was then repositioned before proceeding with the second measurement. Results: The LSC values derived using the CV% values recommended by the ISCD were 5.3% for the lumbar spine and 5.0% for the thigh. The LSC values measured using our bone density equipment were 2.47% for the lumbar spine and 1.61% for the thigh. The LSC value using RMS-SD was 0.031 g/cm² for the lumbar spine and 0.017 g/cm² for the thigh. Conclusion: that the findings confirm that the CV% value measured using our bone density meter and the LSC value using RMS-SD were maintained very stably. This can be helpful for obtaining accurate measurements during bone density follow-up examinations.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.31 no.2D
• /
• pp.323-334
• /
• 2011

In this study, we performed the residual geoid modeling using the FFT and LSC methods in context of application of R-R (Remove and Restore) technique as a general technique for gravimetric geoid model in order to propose the effective way of geoid determination in Korea. For this, a number of data compiled for residual geoid modeling by the multi-band spherical FFT method with Stoke's formula and LSC method as known as statistical method. The geometric geoidal heights obtained from 503 GPS/Levelling data were used for inducing the various elements and proper computation process which should be considered for improving the accuracy of residual geoid modeling. Finally, we statistically compared the results of residual geoid heights between FFT and LSC methods and reviewed then the proper way of residual geoid modeling to the region of Korea. As the results of comparison, LSC method is not suitable for residual geoid modeling in Korea due to the noise and lack of gravity observations and the effects of local characteristics, while FFT method by applying Stokes' integral with proper cap size and modified kernel which provides the better accuracy of residual geoid heights up to 10 cm more than those of LSC method.

• Annals of Hepato-Biliary-Pancreatic Surgery
• /
• v.28 no.2
• /
• pp.214-219
• /
• 2024

Backgrounds/Aims: Open cholecystectomy is becoming obsolete and laparoscopic cholecystectomy has become the treatment of choice in gallstone diseases. Difficult gallbladders are encountered whenever there is a frozen calot's triangle, obliterated cystic plate, or both. Rather than converting to open procedure, there has been a growing preference for laparoscopic subtotal cholecystectomy (LSC) during difficult gallbladders. This study aimed to assess the advantages, indications, and viability of LSC in difficult gallbladders. Methods: The study included patients undergoing laparoscopic cholecystectomy in NIMS Hospital, Jaipur, from January 2021 to January 2023. Data of the patients who underwent LSC for difficult gallbladders included demographics, comorbidities, operative time, conversion to open cholecystectomy, length of hospital stay, and complications. LSC was classified into three types depending on the part of the gallbladder remnant. Results: A total of 728 patients underwent laparoscopic cholecystectomy. Among them, 41 patients (5.6%) were attempted for LSC. However, one patient was converted to an open procedure and the rest 40 underwent LSC. LSC was divided into 3 types, 4 patients underwent LSC type I, 34 patients underwent type II, and 2 patients type III. The average operating time and postoperative length of hospital stay were 86.2 minutes and 2.1 days, respectively. Two patients had surgical site infection. No patient had a bile leak and none required intensive care unit care. Conclusions: LSC is a safe and feasible option for use in difficult gallbladders.

• Korean Chemical Engineering Research
• /
• v.46 no.6
• /
• pp.1135-1141
• /
• 2008

The $La_{1-x}(Ca\;or\;Sr)xCrO_3$(x=0 and 0.25) interconnect materials for high temperature electrolysis of steam were investigated in views of sinterability and electrical conductivity. $LaCrO_3$, $La_{0.75}Ca_{0.25}CrO_3$ (LCC), and $La_{0.75}Sr_{0.25}CrO_3$ (LSC) powders were synthesized by coprecipitation method. Crystal structure was confirmed by X-ray diffraction (XRD). The sintering characteristics were analyzed by relative density and scanning electron microscopy. The electrical conductivity was measured by a DC four probe method. From the analyses of relative densities, it was found that the doped $LaCrO_3$ showed better sinterability than $LaCrO_3$ and the those sinterability increased with decrease of those particle sizes. The XRD results at different sintering temperatures for LCC and LSC revealed that the sinterability is closely related to the second phase transformation, that is, the second phase melting above $1,300^{\circ}C$ for LCC and $1,400^{\circ}C$ for LSC significantly promotes the sinterability. In case of electrical conductivities of LCC and LSC, which had a similar relative density, LCC showed better electrical conductivity than LSC.

• Journal of the Korea Society of Computer and Information
• /
• v.11 no.6 s.44
• /
• pp.49-57
• /
• 2006

While developing a complex reactive software system. it is very important to analyze the user requirement and reflect it to the developed system. Therefore understanding the need of users precisely and promptly is the key to the successful software system development. Among several requirement specification languages, message sequence charts (MSCs), also known as sequence diagrams in UML are the most widely used scenario notation. Live Sequence Charts (LSCs) are a variant ot MSCs, characterized by its message abstraction facility and the modality of scenarios. In this paper, I define the formal semantics of LSC specification including the essential language constructs such as pre-charts, variables, assignment and conditions. The range of the formalized LSC language has been broadened, and the scope of the formalized semantics is much closer to the complete LSC specification.