• 한국소프트웨어감정평가학회 논문지
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• 제17권1호
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• pp.51-58
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• 2021

최근 주목받고 있는 마이크로서비스는 독립적으로 개발될 뿐만 아니라 독립적으로 실행 및 배포가 가능하다는 장점 때문에, 클라우드 컴퓨팅 환경에서 보다 유연한 확장과 효율적인 협력을 보장할 수 있다. 이러한 영향으로 최근 마이크로서비스지향 애플리케이션 환경으로의 전환이 급격히 증가하고 있다. 마이크로서비스의 도입을 위해서는 무엇보다 모노리식 아키텍처로 구축된 단일 애플리케이션의 구성요소를 마이크로서비스 단위로 식별하는 문제가 선결되어야 한다. 본 논문에서는 레거시 시스템으로부터 마이크로서비스 식별의 문제를 알고리즘 기반으로 해결하기 이한 접근방법을 제안한다. 코드의 메타정보를 이용하여 그래프를 생성하고 클러스터링 알고리즘을 적용하여 마이크로서비스 후보를 추출한다. 추출된 마이크로서비스 후보에 대해 메트릭을 이용하여, 모듈화 품질을 평가한다. 또한 제안된 식별 방법의 효과를 검증하기 위해 벤치마크를 위해 많이 사용되는 공개 소프트웨어의 코드를 이용하여 후보 서비스를 도출하고, 메트릭을 이용하여 모듈화 수준을 평가한다. 결과적으로 좀더 작은 단위의 마이크로서비스로 식별해 내면서 모듈품질을 향상시키는 결과를 확인할 수 있다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.27-28
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• 2014

Beauveria bassiana (Cordycipitaceae, Hypocreales, Ascomycota) is an anamorphic fungus having a potential to be used as a biological control agent because it parasitizes a wide range of arthropod hosts including termites, aphids, beetles and many other insects. A number of bioactive secondary metabolites (SMs) have been isolated from B. bassiana and functionally verified. Among them, beauvericin and bassianolide are cyclic depsipeptides with antibiotic and insecticidal effects belonging to the enniatin family. Non-ribosomal peptide synthetases (NRPSs) play a crucial role in the synthesis of these secondary metabolites. NRPSs are modularly organized multienzyme complexes in which each module is responsible for the elongation of proteinogenic and non-protein amino acids, as well as carboxyl and hydroxyacids. A minimum of three domains are necessary for one NRPS elongation module: an adenylation (A) domain for substrate recognition and activation; a tholation (T) domain that tethers the growing peptide chain and the incoming aminoacyl unit; and a condensation (C) domain to catalyze peptide bond formation. Some of the optional domains include epimerization (E), heterocyclization (Cy) and oxidation (Ox) domains, which may modify the enzyme-bound precursors or intermediates. In the present study, we analyzed genomes of B. bassiana and its allied species in Hypocreales to verify the distribution of NRPS-encoding genes involving biosynthesis of beauvericin and bassianolide, and to unveil the evolutionary processes of the gene clusters. Initially, we retrieved completely or partially assembled genomic sequences of fungal species belonging to Hypocreales from public databases. SM biosynthesizing genes were predicted from the selected genomes using antiSMASH program. Adenylation (A) domains were extracted from the predicted NRPS, NRPS-like and NRPS-PKS hybrid genes, and used them to construct a phylogenetic tree. Based on the preliminary results of SM biosynthetic gene prediction in B. bassiana, we analyzed the conserved gene orders of beauvericin and bassianolide biosynthetic gene clusters among the hypocrealean fungi. Reciprocal best blast hit (RBH) approach was performed to identify the regions orthologous to the biosynthetic gene cluster in the selected fungal genomes. A clear recombination pattern was recognized in the inferred A-domain tree in which A-domains in the 1st and 2nd modules of beauvericin and bassianolide synthetases were grouped in CYCLO and EAS clades, respectively, suggesting that two modules of each synthetase have evolved independently. In addition, inferred topologies were congruent with the species phylogeny of Cordycipitaceae, indicating that the gene fusion event have occurred before the species divergence. Beauvericin and bassianolide synthetases turned out to possess identical domain organization as C-A-T-C-A-NM-T-T-C. We also predicted precursors of beauvericin and bassianolide synthetases based on the extracted signature residues in A-domain core motifs. The result showed that the A-domains in the 1st module of both synthetases select D-2-hydroxyisovalerate (D-Hiv), while A-domains in the 2nd modules specifically activate L-phenylalanine (Phe) in beauvericin synthetase and leucine (Leu) in bassianolide synthetase. antiSMASH ver. 2.0 predicted 15 genes in the beauvericin biosynthetic gene cluster of the B. bassiana genome dispersed across a total length of approximately 50kb. The beauvericin biosynthetic gene cluster contains beauvericin synthetase as well as kivr gene encoding NADPH-dependent ketoisovalerate reductase which is necessary to convert 2-ketoisovalarate to D-Hiv and a gene encoding a putative Gal4-like transcriptional regulator. Our syntenic comparison showed that species in Cordycipitaceae have almost conserved beauvericin biosynthetic gene cluster although the gene order and direction were sometimes variable. It is intriguing that there is no region orthologous to beauvericin synthetase gene in Cordyceps militaris genome. It is likely that beauvericin synthetase was present in common ancestor of Cordycipitaceae but selective gene loss has occurred in several species including C. militaris. Putative bassianolide biosynthetic gene cluster consisted of 16 genes including bassianolide synthetase, cytochrome P450 monooxygenase, and putative Gal4-like transcriptional regulator genes. Our synteny analysis found that only B. bassiana possessed a bassianolide synthetase gene among the studied fungi. This result is consistent with the groupings in A-domain tree in which bassianolide synthetase gene found in B. bassiana was not grouped with NRPS genes predicted in other species. We hypothesized that bassianolide biosynthesizing cluster genes in B. bassiana are possibly acquired by horizontal gene transfer (HGT) from distantly related fungi. The present study showed that B. bassiana is the only species capable of producing both beauvericin and bassianolide. This property led to B. bassiana infect multiple hosts and to be a potential biological control agent against agricultural pests.

• 한국환경교육학회지:환경교육
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• 제7권1호
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• pp.46-87
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• 1994

Our country has been consuming a huge amount of energy in the course of industrialization and its demand is expected to increase enormously in the future. However, the deposits of energy resources are so limited that the settlement of energy problem comes up the essential subject. To solve the energy problem, it is requested that new resources to gain energy stably should be developed and also energy should be economized and used effectively. The effective use of energy and an the wisdom of economy in energy are requested to everybody and these things should be habitualized from very young age through education. Nevertheless, almost every school in our country hasn’t been concerned about energy education. Even though they have a concern, they are very short of the energy education materials and the quality of the materials is not so good. Therefore it is very meaningful to the settlement of energy problem of the country to make the students who will lead our country to make the students who will lead our country in the future realize the seriousness of energy problem and to provide them the necessary knowledge and methods to solve this problem so that they practice those things in everyday life. Having these necessities, this research, supported by The Korea Energy Management Corporation(KEMCO), was performed for 8 months from April 17, 1994 to December 17, 1994. Many peoples participated in this study such as 30 staffs of researchers and authors, 5 staffs of photographers and illustrators, and 3 VCR program producers developing an energy education material set for middle school students that includes a printed material for student, a diskette for computer simulation, a teacher's guidebook, VCR material and its guidebook. The following main development direction was established : First, the material for student should be consisted of units that let students know the seriousness of energy problem. Second, the focus should be put on the necessary method and practice to economize energy actually in real life based on the basic knowledge learned in elementary school. Third, material for student should be consisted of modules to be student activity-oriented teaching-learning rather than lecture-oriented one. The activity, to maximize student's interests, should be presented in various forms such as experiments, investigation, play, data interpretation, computer simulation, visits, expression and appreciation, etc. To develop the energy education materials for middle school students, a research plan was made first. After literature review about domestic and foreign energy education materials, several research trips home and abroad, and discussion meetings, the basic theory of energy education such as the principle, objective, contents, teaching-learning method, and evaluation method was established. Material for student was developed through the following procedures : The activities in the existing energy education materials were analysed and were divided into four categories related to energy using places of home, school, community, and country, and which were again divided into three categories related to time of past, present, and future, Considering these division, nine modules which are structure units of material for student were chosen, Each module comprises 2-4 activities. Totally 31 activities were designed in this way. The syllabi were made out for each activity and writing was asked for to experts related to each activity after several discussions and revision. To complement the draft, another several discussions and revision were also made on it and then pictures and illustrations were asked for. All these procedures complete the material for student, titled ; Energy Inquiry of Middle School Students', which totals 129 pages and is all in color. As the manuscript of material for student was fixed, writing for teacher's guidebook was asked for to the same writers. The draft of teacher's guidebook was also complemented through the several concentrated works and discussions. Teacher's guidebook focused on the teaching-learning principle and methods of energy education and on the concrete instruction cases for effective instruction of material for student. It is organized with two parts : the one is 'general outline' which introduces theoretical contents and the other is 'details' which are practically helpful to teaching-learning. It is totally 131 pages including both 'general outline' and 'details'. The VCR material and its guidebook consist of contents that cultivate the good attitude trying to economize energy and raise student's interests with a purpose of strong motivation to recognize the necessity of economy and practice it. After establishing development direction of VCR material through discussion meetings and research trips, its script was made by relevant experts. Then the script was also reviewed two times. The drafted VCR material made by a video material developing expert was examined and modified by previews twice. After completion of VCR material, the VCR guidebook was made. All these procedures led to the development of VCR material which runs 20 minutes in VHS type. The VCR guidebook shows a production purpose of the program, structure of contents, evaluation methods, and contents of the program in detail to give help to instructors when they use this VCR material, When these energy education materials are used, it is desirable that the VCR material should be presented first to induce student's motive, and then material for student is introduced Since the material for student is composed of activity-oriented modules and each module is independent one another in general, and each activity is, too. the necessary module or activity can be chosen and utilized in any order according to school or class conditions. This energy education materials will contribute to the development of student's ability to solve energy problem in everyday life and teacher's ability to teach the fundamental knowledge and method in solving energy problem.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제20권2호
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• pp.82-89
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• 2009

Objectives: Children with attention-deficit hyperactivity disorder(ADHD) often have difficulties in social behavior. The aim of this study was to evaluate the effectiveness of a short-term training program for improving social skills, self-perception and attention deficits. Methods: The subjects were nine children diagnosed with ADHD with(or without) other mental disorders using the Diagnostic Interview Schedule for Children(DISC-ADHD) module. Children were given eight sessions of a social skills training program. Parents of children simultaneously participated in their own training which was designed to support their children's generalization of skills. Assessments included child, parent and teacher ratings of social skills, self-perception and attention deficit at baseline and post-treatment. Results: Social skills training led to significant improvements in child-reported measures of self-esteem, in teacher reported measures of social skills, and in parent-reported measures of attention deficit. Conclusion: This study suggests that short-term social skills training programs for children with ADHD may improve their social skills, self-perception and attention deficits.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2002년도 창립10주년기념 및 국립독성연구원 의약품동등성평가부서 신설기념 국재학술대회:생물학적 동등성과 의약품 개발 전략을 위한 국제심포지움
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• pp.113-113
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• 2002

Phylogenetically conserved Bcl-2 family proteins play a pivotal role in the regulation of apoptosis from virus to human. Members of the Bcl-2 family consist of antiapoptotic proteins such as Bcl-2, Bcl-xL, and Bcl-w, and proapoptotic proteins such as BAD, Bax, BOD, and Bok. It has been proposed that anti- and proapoptotic Bcl-2 proteins regulate cell death by binding to each other and forming heterodimers. A delicate balance between anti- and proapoptotic Bcl-2 family members exists in each cell and the relative concentration of these two groups of proteins determines whether the cell survives or undergoes apoptosis. Mcl-1 (Myeloid cell :leukemia-1) is a member of the Bcl-2 family proteins and was originally cloned as a differentiation-induced early gene that was activated in the human myeloblastic leukemia cell line, ML-1 . Mcl-1 is expressed in a wide variety of tissues and cells including neoplastic ones. We recently identified a short splicing variant of Mcl-1 short (Mcl-IS) and designated the known Mcl-1 as Mcl-1 long (Mcl-lL). Mcl-lL protein exhibits antiapoptotic activity and possesses the BH (Bcl-2 homology) 1, BH2, BH3, and transmembrane (TM) domains found in related Bcl-2 proteins. In contrast, Mcl-1 S is a BH3 domain-only proapoptotic protein that heterodimerizes with Mcl-lL. Although both Mc1-lL and Mcl-lS proteins contain BH domains fecund in other Bcl-2 family proteins, they are distinguished by their unusually long N-terminal sequences containing PEST (proline, glutamic acid, serine, and threonine) motifs, four pairs of arginine residues, and alanine- and glycine-rich regions. In addition, the expression pattern of Mcl-1 protein is different from that of Bcl-2 suggesting a unique role (or Mcl-1 in apoptosis regulation. Tankyrasel (TRF1-interacting, ankyrin-related ADP-related polymerasel) was originally isolated based on its binding to TRF 1 (telomeric repeat binding factor-1) and contains the sterile alpha motif (SAM) module, 24 ankyrin (ANK) repeats, and the catalytic domain of poly(adenosine diphosphate-ribose) polymerase (PARP). Previous studies showed that tankyrasel promotes telomere elongation in human cells presumably by inhibiting TRFI though its poly(ADP-ribosyl)action by tankyrasel . In addition, tankyrasel poly(ADP-ribosyl)ates Insulin-responsive amino peptidase (IRAP), a resident protein of GLUT4 vesicles, and insulin stimulates the PARP activity of tankyrase1 through its phosphorylation by mitogen-activated protein kinase (MAPK). ADP-ribosylation is a posttranslational modification that usually results in a loss of protein activity presumably by enhancing protein turnover. However, little information is available regarding the physiological function(s) of tankyrase1 other than as a PARP enzyme. In the present study, we found tankyrasel as a specific-binding protein of Mcl-1 Overexpression of tankyrasel led to the inhibition of both the apoptotic activity of Mel-lS and the survival action of Mcl-lL in mammalian cells. Unlike other known tankyrasel-interacting proteins, tankyrasel did not poly(ADP-ribosyl)ate either of the Mcl-1 proteins despite its ability to decrease Mcl-1 proteins expression following coexpression. Therefore, this study provides a novel mechanism to regulate Mcl-1-modulated apoptosis in which tankyrasel downregulates the expression of Mcl-1 proteins without the involvement of its ADP-ribosylation activity.

• 한국수자원학회논문집
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• 제43권11호
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• pp.995-1009
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• 2010

기후변화에 의해 집중호우의 빈도 및 강도가 증가하고 지속적인 유역개발에 따른 토지이용의 증가는 토양침식 및 토사유출로 인한 재해 및 환경문제를 야기한다. 현재 광범위하게 사용되고 있는 토양침식량 산정기법은 대부분 대상유역내의 평균 토양침식량을 산출하는 총량적 개념의 경험식이므로 호우기간동안의 침식 및 퇴적의 시 공간적 변화양상을 모의할 수 없다는 한계를 지니고 있다. 따라서 보다 합리적인 유역규모의 강우-유사-유출 메카니즘 해석을 위해서는 기존의 집중형 모의기법을 대체하고 다양한 기상학적/지형학적 정보를 활용할 수 있는 물리적 기반의 분포형 모형이 요구된다. 본 연구에서는 사면의 지표 및 지표하 흐름을 고려한 유출모의 모듈과 단위수류력 이론을 기반으로 하는 유사유출 모의모듈을 결합한 분포형 강우-유사-유출 모형을 확장개발하고, 용담댐 상류부의 천천유역에 적용하여 모형의 재현성 평가를 수행하였다. 수문곡선의 모의 결과 모형의 재현성은 우수하였으며, 유사량곡선의 경우 첨두부분에서 과소선정되는 경향이 나타났다. 또한, 지표면 유동거리 및 국부경사에 따른 침식 및 퇴적의 공간분포를 분석한 결과, 침식된 토사는 비교적 경사가 완만한 하천주변에 퇴적되었으며, 강우량과 침식 및 퇴적의 공간분포의 분석결과, 강우량의 증가에 따라 침식량이 증가하였으며, Thiessen망 유역별로 침식 및 퇴적 분포는 상이하게 나타났다.

• 한국통신학회논문지
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• 제36권12B호
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• pp.1556-1564
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• 2011

의료 기술의 발달로 인한 평균 수명의 연장은 인구 고령화, 의료비 증가, 전문 의료 인력 부족 등 다양한 사회 문제를 야기 시켰다. 이에 따라 고도화 된 IT 기술을 의료 서비스 시장에 융합하여 최소한의 비용으로 삶의 질을 향상시킬 수 있는 유헬스 연구가 진행되고 있다. 하지만 대부분의 관련 연구들이 의료 표준 기술을 지원하지 않아 기존 의료 기기 간 호환성 및 확장성이 없고, 의료 기관 마다 다른 메시지 포맷을 사용하여 상호운용성을 보장 받을 수 없다. 따라서 본 논문에서는 현재 상용화된 비표준 개인 건강 기기에 의료 기기간 표준 전송 기술인 IEEE 11073을 지원할 수 있는 확장 모듈을 개발하고 IEEE 11073을 이용하여 개인 건강 기기로부터 수집된 정보를 전송받아 쉽고 간편하게 통합 관리 할 수 있는 스마트폰 기반의 통합 매니저를 개발하였다. 또한 매니저에 저장된 정보를 표준 데이터 포맷인 HL7을 이용하여 의료 센터로 전송함으로써 표준 기술이 지원되는 모든 의료 기관으로부터 실시간 의료 서비스를 제공 받을 수 있는 유헬스 시스템을 설계/구현하였다.

• 한국수학교육학회지시리즈C:초등수학교육
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• 제24권4호
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• pp.217-231
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• 2021

'거꾸로 수업'으로 번역되는 플립드 러닝은 여러 연구와 매체들을 통하여 그 효과성을 인정받고 있으나 활용 시 교사에게 주어지는 지나친 수업 준비 부담의 한계를 지니고 있다. 그러나 온라인 러닝이 활성화됨에 따라 한국교육방송공사(EBS)가 제공하는 플랫폼(EBSmath)을 활용하면, 거꾸로 수업에 대한 교사의 수업 부담을 극복할 수 있다. 본 연구에서는 거꾸로 수업 모델로 EBSmath를 활용하여 '비와 비율'의 학습하였을 때, 초등학교 6학년 학생들의 수학 학습 성취도와 수학적 성향에 미치는 영향을 분석하였다. 이 중 수학 학습 성취도는 '개념 이해'와 '문제해결력'으로 구분하여 분석하였다. 연구 결과 EBSmath를 활용한 거꾸로 수업은 학생들의 비와 비율 문제 해결에 긍정적인 영향을 미쳤으며, 수학적 성향 중 '의지' 면에서 통계적으로 유의한 변화가 확인되었다.

• 한국산학기술학회논문지
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• 제22권5호
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• pp.162-170
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• 2021

스마트시티 리빙랩은 지역주민을 포함한 다양한 이해관계자와의 창의적 소통으로 도시의 솔루션을 만들어가는 지역혁신 플랫폼으로서 점차 중요성이 증대하고 있다. 특히, 2018년 1월 대통령 직속 산하 기구인 4차산업혁명위원회는 「도시혁신 및 미래성장동력 창출을 위한 스마트시티 추진전략」 발표를 통해 스마트시티에서의 시민참여와 역할에 대한 중요성을 강조함으로써 다양한 공공사업 분야에 리빙랩이 적용되는 계기가 되었다. 이에 중앙부처와 지자체는 시민참여형 스마트시티 건설을 위해 다양한 유형의 리빙랩을 추진하고 있지만 체계적인 단계별 리빙랩 수행을 위한 구조화된 절차와 방법론 부재로 인해 선행 사례를 참조하여 임시방편적으로 추진하고 있는 실정이다. 이에 본 연구는 공공주도형 스마트시티 리빙랩의 체계적인 접근 및 실행 방안을 마련하기 위해 혁신 디자인 방법론인 더블디자인 다이아몬드 프레임워크와 디자인 씽킹 프로세스를 비롯해 국토계획 표준품셈에서 제시하고 있는 스마트도시 시민 리빙랩의 업무 프로세스를 융복합하여 전주기형 스마트시티 리빙랩 모델을 개발하였다. 본 연구에서 개발된 전주기형 스마트시티 리빙랩 모델은 프레임워크, 모듈, 프로세스, 방법론 등 리빙랩을 접근하고 실행하기 위해 필요한 4개의 구성요소를 다루고 있다. 향후 스마트시티 리빙랩을 추진하는데 있어 본 모델을 참고 자료로서 활용되길 기대한다.

• 국제학술발표논문집
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• The 9th International Conference on Construction Engineering and Project Management
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• pp.1243-1244
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• 2022

In recent years, the growing interest in off-site construction has led to factories scaling up their manufacturing and production processes in the construction sector. Consequently, continuous large-scale site monitoring in low-variability environments, such as prefabricated components production plants (precast concrete production), has gained increasing importance. Although many studies on computer vision-based site monitoring have been conducted, challenges for deploying this technology for large-scale field applications still remain. One of the issues is collecting and transmitting vast amounts of video data. Continuous site monitoring systems are based on real-time video data collection and analysis, which requires excessive computational resources and network traffic. In addition, it is difficult to integrate various object information with different sizes and scales into a single scene. Various sizes and types of objects (e.g., workers, heavy equipment, and materials) exist in a plant production environment, and these objects should be detected simultaneously for effective site monitoring. However, with the existing object detection algorithms, it is difficult to simultaneously detect objects with significant differences in size because collecting and training massive amounts of object image data with various scales is necessary. This study thus developed a large-scale site monitoring system using edge computing and a small-object detection system to solve these problems. Edge computing is a distributed information technology architecture wherein the image or video data is processed near the originating source, not on a centralized server or cloud. By inferring information from the AI computing module equipped with CCTVs and communicating only the processed information with the server, it is possible to reduce excessive network traffic. Small-object detection is an innovative method to detect different-sized objects by cropping the raw image and setting the appropriate number of rows and columns for image splitting based on the target object size. This enables the detection of small objects from cropped and magnified images. The detected small objects can then be expressed in the original image. In the inference process, this study used the YOLO-v5 algorithm, known for its fast processing speed and widely used for real-time object detection. This method could effectively detect large and even small objects that were difficult to detect with the existing object detection algorithms. When the large-scale site monitoring system was tested, it performed well in detecting small objects, such as workers in a large-scale view of construction sites, which were inaccurately detected by the existing algorithms. Our next goal is to incorporate various safety monitoring and risk analysis algorithms into this system, such as collision risk estimation, based on the time-to-collision concept, enabling the optimization of safety routes by accumulating workers' paths and inferring the risky areas based on workers' trajectory patterns. Through such developments, this continuous large-scale site monitoring system can guide a construction plant's safety management system more effectively.