• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.88-88
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• 2001

• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.68-73
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• 2004

Ferritin is an iron storage protein found in most living organisms. For expression and industrial use, human light chain ferritin (L-ferritin) was cloned from human liver cDNA library and expressed in Pichia pastoris strain GS115. The recombinant L-ferritin in Pichia pastoris was glycosylated. In a fed-batch culture, the cell mass reached about 57 g/l of dry cell weight, and the L-ferritin in the cell was increased to about 95 mg/l after 150 h. In an atomic absorption spectrometry analysis, the intracellular content of iron in the L-ferritin transformant was measured as $1,694{\pm}85\;\mu\textrm{g}g/g$, which is 5.4-fold more than that of the control strain. This L-ferritin transformant could serve as iron-fortified nutrients in animal feed stock.

• Animal cells and systems
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• v.12 no.3
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• pp.143-148
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• 2008

Heterogeneous nuclear ribonucleoprotein E1(hnRNP E1) is one of the primary pre-mRNA binding proteins in human cells. It consists of 356 amino acid residues and harbors three hnRNP K homology(KH) domains that mediate RNA-binding. The hnRNP E1 protein was shown to play important roles in mRNA stabilization and translational control. In order to enhance our understanding of the cellular functions of hnRNP E1, we searched for interacting proteins through a yeast two-hybrid screening while using HeLa cDNA library as target. One of the cDNA clones was found to be human ribosomal protein L18a cDNA(GenBank accession number BC071920). We demonstrated in this study that human ribosomal protein L18a, a constituent of ribosomal protein large subunit, interacts specifically with hnRNP E1 in the yeast two-hybrid system. Such an interaction was observed for the first time in this study, and was also verified by biochemical assay.

• Journal of the Microelectronics and Packaging Society
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• v.8 no.1
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• pp.61-64
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• 2001

VCO(Voltage Contolled Oscillator) is one of the main components governing the size, performance and power consumption of telecommunication devices. As the devices become much smaller, YCO need to hove much smaller size with better characteristics. Buried type passive components of L, C, R were developed previously and the structure of these components are good for minimizing the size of VCO. Our own library of passive components is used in simulation and measurement circuit designed by ourselve. In structure of multi-layered VCO, some components governing the characteristics of VCO are selected and placed on the top of oscilltor for the good tuning process. In resonator part, the stripline structure and low loss glass/ceramic material are used to get higher Q value. In our research, a VCO oscillates in the 2.3~2.36 GHz band is developed.

• Korean Journal of Plant Tissue Culture
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• v.21 no.2
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• pp.111-115
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• 1994

Two different gravity specific cDNA, namely, GSC 13 and GSC 124 with length of 1.34 and 0.67 kilobase pairs, and transcripts of 2.0 and 1.9 kilobase pairs, respectively. were isolated by differential screening and northern hybridization of the total RNA isolated from treated and untreated cultured cells showed that maximum levels of trannscripts were achieved after 4 h of gravity stress at 450, 000 x g for both, GSC 13 and GSC 124, suggesting that these mRNA could be expressed and translated into polyeptites related to the cell to extream gravity stress.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.350-355
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• 2010

Among human antimicrobial peptides (hAMPs), DCD-1L has a broad spectrum of antimicrobial activity over a wide pH range and in high salt concentrations. It offers a promising alternative to conventional antibiotics. The 458-bp-long dermcidin cDNA was amplified by PCR using a human fetal cDNA library as a template. The 147-bp fragment of the MDCD-1L gene encoding an additional methionine residue was subcloned into the pTYB11 vector. Recombinant MDCD-1L was expressed as an intein fusion protein in E. coli, and then purified by affinity chromatography using chitin beads. A small peptide with a molecular mass of about 5 kDa was detected by tricine gel electrophoresis. The recombinant MDCD-1L peptide was purified from the gel and its amino acid sequence was determined by nanoLC-ESI-MS/MS analysis. The initiating amino acid, methionine, remained attached to the N-terminal region of recombinant MDCD-1L. Purified MDCD-1L showed antimicrobial activity against a Micrococcus luteus test strain.

• Journal of Korean Library and Information Science Society
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• v.18
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• pp.217-243
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• 1991

Authority control enables us to retrieve information accurately by coordination and cross reference of headings. It is considered that authority control is needed in machine readable catalogs as well as in conventional catalogs. Taylor, Maxwell and Frost divided automated authority control systems into major vendor and network systems then available into two groups. The first group was defined as a system that processes a library's machine readable catalogs on the tape against an automated authority file. The second group offers an online access to catalogs and authority records. This group was further subdivided into linked or integrated systems and unlinked systems. If authority work is shared with many libraries the costs can be considerably reduced and the quality of catalog data base can be controled by standardization of headings used in participating libraries. The NACO is a project for sharing of authority records started in 1977 by L.C. And LSP furthers the ability to share authority records. A project as the NACO is necessary not only in America but also in the other countries The responsible organizations for national bibliographies of the countries must take much interest in these matters.

• Journal of the Korean Society for information Management
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• v.9 no.2
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• pp.118-153
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• 1992

This study att.empts to conduct a system an;~lysis and dcsign for circt~lation control in a univcr~ity library. In this proccss, structured sysltcm analys15 tcrhn~clucs Iikr data flolv diagrnms. data dictiomiry, and entity - relationship analysis, arc. vmploycd to construct both concqitual and p h p c a l data models of current and new circulation systems. Thc whole design aims at an al~tomntctf c~r.culation system on thc [nlcro-compurcr --basis in a mid dle - sized library.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.200.2-201
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• 2000

No Abstract, See Full Text

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.4
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• pp.286-290
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• 2005

Ascorbate peroxidase (APX) plays a crucial role in the detoxification of hydrogen peroxide. APX activity is maintained significantly higher in the paraquat­treated leaves of the paraquat-tolerant Rehmannia glutinos. This study was conducted to understand structural and regulatory characteristics of APX gene in R. glutinosa. A putative APX cDNA clone (RgAPX1) was isolated from a leaf cDNA library using a partially sequenced expressed sequence tag clone. RgAPX1 is consisted of 1148 bp nucleotides and contains an open reading frame encoding a 250 amino acid-long polypeptide. Deduced RgAPX1 amino acid sequence shares higher sequence similarity to cytosolic APXs. RgAPX1. expression was higher in the leaf than in the flower and root. Southern blot result indicates the presence of one or two RgAPX1-related genes in R. glutinosa genome. RgAPX1 transcription was affected differentially by various stresses and phytohormone. The results indicate that RgAPXl is constitutively expressed in most tissues and its expression is modulated for the immediate and efficient detoxification of $H_2O_2$ under normal and stress conditions.