KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Regulation of Ascorbate Peroxidase Gene Expression in Response to Stresses and Phytohormone in Rehmannia glutinosa

  • Park Myoung Ryoul (Division of Biological Resources Sciences, Chonbuk Nat'l Univ.) ;
  • Park Moon Hee (Division of Biological Resources Sciences, Chonbuk Nat'l Univ.) ;
  • Yoo Nam Hee (Institute of Agricultural Science and Technology, Chonbuk Nat'l Univ.) ;
  • Yu Chang Yeon (Division of Applied Plant Sciences, Kangwon Nat'l Univ.) ;
  • Yun Song Joong (Division of Biological Resources Sciences, Chonbuk Nat'l Univ., Institute of Agricultural Science and Technology, Chonbuk Nat'l Univ.)
  • Published : 2005.09.01
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Abstract

Ascorbate peroxidase (APX) plays a crucial role in the detoxification of hydrogen peroxide. APX activity is maintained significantly higher in the paraquat­treated leaves of the paraquat-tolerant Rehmannia glutinos. This study was conducted to understand structural and regulatory characteristics of APX gene in R. glutinosa. A putative APX cDNA clone (RgAPX1) was isolated from a leaf cDNA library using a partially sequenced expressed sequence tag clone. RgAPX1 is consisted of 1148 bp nucleotides and contains an open reading frame encoding a 250 amino acid-long polypeptide. Deduced RgAPX1 amino acid sequence shares higher sequence similarity to cytosolic APXs. RgAPX1. expression was higher in the leaf than in the flower and root. Southern blot result indicates the presence of one or two RgAPX1-related genes in R. glutinosa genome. RgAPX1 transcription was affected differentially by various stresses and phytohormone. The results indicate that RgAPXl is constitutively expressed in most tissues and its expression is modulated for the immediate and efficient detoxification of $H_2O_2$ under normal and stress conditions.

Keywords

References

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