• 한국환경복원기술학회지
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• 제13권2호
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• pp.42-51
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• 2010

Herbs and shrubs are employed for environmental restoration purposes. Among common herbs and shrubs, few species with low germination rates were selected and studied for enhanced germination rates and decreased germination times via soaking treatment. Rhus chinensis, incubator grown samples treated with the bacterial solution for 72hrs followed by immediate seeding showed the highest germination rate of 26.7% and germination period of 5.7 days, 3 days decrease from the control. Treatment of distilled water (t=3.79, p<0.01), nutrient broth (t=4.44, p<0.00) and bacterial solution (t=4.42, p<0.00) showed highly significant difference. In the case of soil tests, treating in the nutrient broth for 72 hrs followed by immediate seeding yielded the the highest germination rate of 23.3% with 7.3 days to initial germination, a decrease of 14.7 days with respect to the control. All the samples followed by immediate seeding showed significant difference (t=2.13, p<0.05). Incubator grown samples of Lespedeza cyrtobotrya showed different results. The incubator samples suspended for 48 hrs in distilled water followed by immediate seeding and 1 day drying displayed the highest germination rate of 96.7%, surpassing that of the control by 33.4%. The incubator samples treated with the distilled water and nutrient broth showed enhanced germination. But only the samples treated with distilled water and nutrient broth for 48hrs showed the increased germination in soil tests. All the sample treated for 24 hrs followed by immediate seeding or dried for 1 day showed initial germination as early as 1 day in incubator. The initial germinations were shortened in the samples treated with distilled water and nutrient broth for 48hrs in soil tests. Lespedeza cuneata incubator sample treated with nutrient broth for 24 hrs and dried for 1 day exhibited the highest germination rate of 83.3%, a 31.1% improvement over the control. The incubator samples treated with distilled water for 48 hrs (t=4.20, p<0.01) showed effective increase of germination. The treatment of distilled water (t=2.96, p<0.05) and bacterial solution (t=2.24, p<0.05) showed significant difference. The germination rates in soil were less than those of incubator and the control. The incubator samples treated with distilled water and bacterial solution displayed 1 day germination period, shortened by 1.3 days compared to the control. For soil grown samples, the samples treated with distilled water showed delayed initial germination and those treated with nutrient broth for 48hrs and bacterial solution for 72hrs shortened initial germination.

• 대한본초학회지
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• 제28권4호
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• pp.83-92
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• 2013

Objectives : Lespedeza Cuneata has been used to treat leukorrhea, asthma, stomach pain, diarrhea, acute mastitis, in Korean traditional medicine. According to recent studies, Lespedeza Cuneata has antioxidation, hypoglycemia, cell protective, insulin secretion, whitening, corpora cavernosa smooth muscle relaxation and antimicrobial activities, but it has been rarely conducted to evaluate the immuno-biological activity. The present study was examined to evaluate the anti-inflammatory effects of the Lespedeza Cuneata MeOH extract (LCE) in vivo and in vitro. Methods : In vitro, inflammatory mediators, such as cytokines, nitric oxide and prostaglandin $E_2$ were detected after the addition of LPS with or without LCE in Raw 264.7 macrophage cell line. In vivo, anti-edema effect of LCE was determined in the carrageenan-induced paw edema model in rats. Results : In vitro assay, LCE decreased release of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) via suppression of iNOS and COX-2 expression. LCE inhibited the phosphorylation of $I{\kappa}B$ indicating the suppression of NF-${\kappa}B$ pathway. In vivo assay, LCE significantly inhibited the formation of paw edema induced by carrageenan injection in rats. LCE effectively inhibited increases of hind paw skin thickness and inflammatory cell infilterations. Conclusion : These findings demonstrate that LCE has inhibitory effect on inflammatory mediators in LPS-activated Raw 264.7 cells and on paw edema in carrageenan-stimulated rats, showing the possibility of anti-inflammatory use of Lespedeza Cuneata.

• 농업과학연구
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• 제2권2호
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• pp.399-404
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• 1975

두과수엽(荳科樹葉)의 사료적(飼料的) 이용성(利用性)을 검토(檢討)하기 위하여 싸리(Lespedeza spp.), 쪽제비싸리(Amorpha fruticosa) 및 아카시아 (Robinia pseudo acacia)를 대전시(大田市) 및 대덕군(大德郡) 각(各) 지역(地域)에서 생육시기별(生育時期別)로 수집(蒐集)하고 수엽(樹葉)의 생산성(生産性), 생육시기별(生育時期別) 성분함량(成分含量)의 변화(變化), 기호성등(嗜好性等)을 시험(試驗) 고찰(考察)한 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 수엽(樹葉)의 생산성(生産性)은 싸리에 비하여 쪽제비싸리 및 아카시아가 높았으며 쪽제비싸리는 8월에, 아카시아는 9월에 보다 높은 수량(收量)을 나타내었다. 2. 조단백질(粗蛋白質) 함량(含量)은 싸리가 12.60%, 쪽제비싸리가 18.63%, 아카시아는 15.87%를 함유(含有)하여 쪽제비싸리가 가장 높았으며 생육시기(生育時期)가 진행(進行)됨에 따라 함유량(含有量)은 점차 감소(減少)되었다. 3. 조지방(粗脂肪) 함량(含量)은 싸리가 9.00%, 쪽제비싸리가 5.85%, 아카시아가 4.70%를 나타내고 생육기간(生育期間)이 진행됨에 따라 점차 증가(增加)되었다. 4. 조직유(組織維) 함량(含量)은 싸리가 14.79%, 쪽제비싸리가 12.61%, 아카시아는 9.80%를 나타내어 아카시아가 가장 적었으며 생육시기(生育時期)가 진행됨에 따라 점차 증가 되었다. 5. 가용무질소물(可溶無窒素物) 함량(含量)은 아카시아가 53.01%로서 가장 높았으며 생육이 진행됨에 따라 점차 감소되었다. 6. 조회분(粗灰分) 함량(含量)은 싸리가 4.40%, 쪽제비싸리가 4.98%, 아카시아가 5.27%로서 아카시아가 가장 높았으며 생육시기(生育時期)에 따른 함량변동(含量變動)은 일정하지 않았다. 7. 두과수엽(荳科樹葉)의 기호성(嗜好性)은 싸리, 아카시아, 쪽제비싸리의 순으로 좋았고 생엽(生葉)에 비하여 건엽(乾葉)이 더 우수한 기호성(嗜好性)을 나타내었다.

• 한국식품영양과학회지
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• 제43권4호
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• pp.516-521
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• 2014

야관문 추출물의 피부재생능력을 알아보고, 창상을 유발한 실험동물 모델에서 창상 치유 효과를 검증하고자 6개 군(NO, CO, PC, LCL, LCM, LCH)으로 나누어 5주간 실험하였다. Elastase 저해 활성을 평가한 결과 LCM군에서 항산화제인 BHA보다 2.7%($52.39{\pm}4.52$ vs $53.88{\pm}1.85$) 낮은 수치를 나타내었으며, collagenase 저해 활성을 평가한 결과 LCM군에서 BHA보다 7%($37.68{\pm}2.91$ vs $35.19{\pm}7.80$) 더 높은 활성을 나타내었다. 창상유발 21일에 LCL군, LCM군 및 LCH군의 왼쪽 창상 면적은 CO군과 비교하여 각각 54.2%, 53.5% 및 48.7% 현저한 창상면적 감소를 보였다. 이로서 야관문 추출물은 피부상피조직의 재생과 교원질의 합성을 도와 외과적 창상에 치료 효과가 있는 것으로 판단된다.

• 대한한의학회지
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• 제31권4호
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• pp.79-100
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• 2010

Objective: Production of ROS from glucose toxicity results in injury of pancreatic $\beta$-cells in diabetes models. This study was undertaken to examine the influence of Lespedeza Cuneata extract (LCE) on cytoprotective effects on glucose toxicity, insulin secretion and gene expression in RIN-m5F cells. Methods: First, we measured LCE's antioxidant activity by DPPH free radical-scavenging activity and SOD activity. After the various concentrations of LCE were added to the RIN-m5F cells, we measured cell viability with glucose stimulation by MTT assay and glucose-stimulated insulin secretion. We analyzed gene expression with Agilent whole mouse genome 44K oligo DNA microarray and searched for related pathways in KEGG (Kyoto Encyclopedia of Genes and Genomes). Lastly we measured INS-1, INS-2, INS-R, IRS-1, IRS-2, IRS-3, GLP-1R, and GLP-2R mRNA expression by real time RT-PCR. Results: Free radical-scavenging activity, SOD activity and insulin secretion increased dependent on LCE concentration, but LCE did not show considerable cytoprotective effect on RIN-m5F cells. More than twice expressed gene was 6362 in Oligo DNA chip. In KEGG, the most related pathway was the metabolic pathway. In the insulin signaling pathway, up expressed genes were Irs1, Mapk8, Akt1, and Lipe and down expressed genes were Rhoq, Fbp2, Prkar2b, Gck, and Prkag1. In real time RT-PCR, IRS-2, and IRS-3 expression increased significantly compared to the control group on LCE $12{\mu}g/m{\ell}$ concentration and GCK expression decreased significantly compared to the control group. Conclusions: These results show that LCE encourages insulin secretion and insulin metabolism by complicated gene mechanisms. Further mechanism study and clinical study seem to be necessary about Lespedeza Cuneata.

• 한국산림과학회지
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• 제94권3호통권160호
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• pp.146-152
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• 2005

훼손된 비탈면의 조림에 균근균의 이용가능성을 타진하기 위하여, 땅찌만가닥버섯(Lyophyllum shimeji)의 배양학적 특성 및 인공접종에 의한 참싸리(Lespedeza cyrtobotrya)에서의 균근 형성 특성을 조사하였다. L. shimeji는 MP (1% malt extract, 0.1% peptone, 1% glucose, 1.5% agar)배지에서 균사 생장이 가장 왕성하였으며, 균사 최적 배양 온도 및 pH는 각각 $25^{\circ}C$ 및 pH 6 이었다. 인공접종에 의한 참싸리에서의 균근 형성 특성을 조사한 결과, L. shimeji는 Hartig net을 형성하는 외생균근의 특성을 나타내었으며, 균근 접종 실생 묘목 뿌리의 생장율은 대조구에 비하여 우수하였다. 균근 접종 실생 묘목을 비탈면에 이식하였을 때의 생존율 및 건중량은 각각 62% 및 850 mg/본 이었으나, 균근을 접종하지 않은 실생 묘목의 생존율 및 건중량은 각각 11% 및 430 mg/본 이었다.

• 한국방재학회 논문집
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• 제9권3호
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• pp.81-87
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• 2009

본 연구에서는 산림 내 가연물의 산불위험성을 판단하기 위하여 영동지역에서 자생하는 관목류(생강나무, 조록싸리나무, 초피나무, 산초나무, 개암나무)의 생엽을 대상으로 발화점시험기, 콘칼로리미터, 연기밀도시험기를 이용하여 연소특성을 고찰하였다. 조록싸리나무는 발화온도가 가장 낮고 착화시간이 가장 빨랐으며, CO와 $CO_2$의 평균방출농도가 가장 높은 것으로 나타났다. 초피나무는 가장 많은 열량을 방출하였으며, 산초나무는 가장 많은 연기를 발생한 것으로 나타났다. 따라서 산불발생 시 조록싸리나무는 다른 수종에 비하여 착화가 가장 용이하고 초피나무는 확산 및 화재강도가 가장 클 것으로 판단되며, 산초나무와 조록싸리나무는 다량의 연기발생에 따라 피난이 어려울 것으로 사료된다.

• 한국산림과학회지
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• 제30권1호
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• pp.42-49
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• 1976

참싸리 부위별 과산화동위효소형의 변이를 알고저 전기영동법에 의하여 효소형을 조사한 바, 동일 개체에서 동시에 채취한 동일부위의 과산화동위효소형의 변이는 없었으며 공동출현 "밴드"는 부위별로 차이가 있어 잎, 인피, 세근에서는 7번"밴드"자방에서는 35번"밴드"가 나타났으며 부위별 개체별 과산화동위효소형의 변이가 있었고 성숙된 잎을 제외하고는 개체간 과산화동위효소형의 색정농도에 차이가 있었다.

• 한국약용작물학회지
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• 제19권5호
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• pp.368-372
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• 2011

In this study, we investigated on antioxidative activity and nitric oxide production inhibitory activity of various solvent extracts of Lespedeza bicolor. The total polyphenol content of the methanol extract was 192.6 mg/g and flavonoid content of the acetone extract was 40.6 mg/g, as the highest content. In DPPH radical scavenging ability, $SC_{50}$ values of the ethanol and methanol extract were exhibited $0.69mg/m{\ell}$ and $0.89mg/m{\ell}$, respectively. However, in nitric oxide(NO) scavenging ability, $SC_{50}$ values of the acetone was exhibited $0.72mg/m{\ell}$ as the highest activity. Moreover, the acetone extract showed strong NO production inhibitory effect in lipopolysaccharide(LPS)-stimulated Raw 264.7 cell. In the cytotoxicity measurement by MTT assay, the extracts were exhibited Raw 264.7 cell viabilities of 92.57~129.04% as nontoxic result in concentration of $65{\sim}650{\mu}g/m{\ell}$. As a result, the acetone extract of L. bicolor could be applicable to functional materials for anti-inflammatory related fields.

• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.988-994
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• 2010

The EtOAc fraction of Lespedeza cyrtobotrya showed mushroom tyrosinase inhibitory and radical scavenging activities. Four active compounds were isolated based on Sephadex LH-20 chromatography and HPLC, and the structures were elucidated, on the basis of their LC-MS and NMR spectral data, as 2-(2,4-dihydroxyphenyl)-6-hydroxybenzofuran (1), eriodictyol-7-O-glucopyranoside (2), haginin A (3), and dalbergioidin (4), respectively. Compound (1) showed mushroom tyrosinase inhibitory activity with an $IC_{50}$ value of $5.2\;{\mu}M$ and acted as a competitive inhibitor. Furthermore, $37.3\;{\mu}M$ of compound 1 reduced 50% of the melanin content on human melanoma (MNT-1) cells. The radical scavenging activities of compounds 1, 2, 3, and 4 were shown to have $IC_{50}$ values of 11.0, 24.5, 9.0, and $36.5\;{\mu}M$, respectively, in an ABTS system and $IC_{50}$ values of 42.7, 36.0, 37.7, and $61.7\;{\mu}M$, respectively, in a DPPH system. The mushroom tyrosinase inhibitory activity of the EtOAc fraction of Lespedeza cyrtobotrya was contributed by compounds 1, 3, and 4, and its radical scavenging activity was contributed by compounds 1-4.